| Definition | Bacteroides thetaiotaomicron VPI-5482 chromosome, complete genome. |
|---|---|
| Accession | NC_004663 |
| Length | 6,260,361 |
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The map label for this gene is pulA [H]
Identifier: 29350097
GI number: 29350097
Start: 6150497
End: 6152464
Strand: Reverse
Name: pulA [H]
Synonym: BT_4689
Alternate gene names: 29350097
Gene position: 6152464-6150497 (Counterclockwise)
Preceding gene: 29350113
Following gene: 29350096
Centisome position: 98.28
GC content: 43.24
Gene sequence:
>1968_bases ATGAGAGAGTTGAAAAATCATCCGTCCCCGGACCAGTTGGATTCGTATGACAAGTATCCGTGCTATTATGAGAACGATTT GGAATTAGTCTATACACCCCAGCAATCCATATTTACGCTATGGGCACCAAGTGCCGATAATGTCCGTTTAAACCTGTATT CATCCGGCGAAGCGGGAGATCCGGAAGAACAACTGGAAATGGACATAGCCGACAATGGGACCTGGTGTATCCATATAGAC CGTGATCTGAAAGGTTCTTTCTACACCTTCCAGATAGAAAAAGATGGAAAATGGCTGGACGAAACACCCGGTATCTGGGC AAAAGCAGTCGGCATCAATGGCAATCGCGCTGCTGTAATCGACTGGAACGAGACAAATCCCGAAGGTTGGGAATCAGACC AGTCACCCGAACTGAAAATGTATTCCGATATCATTCTTTATGAACTTCATCACCGCGATTTTAGTATTGATCCAAACTCA GGAATTGAACATAAGGGGAAGTTTCTTGCTCTGACAGAGACCGGAACTAAAACTCCCGAAGGAGAATCTTCCGGACTGGA TCATTTAAAAGAATTGGGAGTAACGCATATACACATCCTTCCTTCATTCGATTTCGCCACAATCGATGAAAGGAAATTGG AGAAAAACAAATACAATTGGGGATATGACCCGAAGAATTATAATGTACCAGATGGAAGTTACTCCACCGATCCTGTCACC CCCACCACCCGTATCCGCGAATTTAAAGAGATGGTAAAAAGCCTCCATCAAAATGGTTTTCGTATTGTACTCGATGTGGT TTATAACCATACAGCCTCAACCGAGCATTCTAATTTTGACCTAACGGTTCCGGGATATTTTTACCGCCAGAATCCCGACG GTTCCTATTCAAATGCTTCCGGGTGTGGAAATGAGACAGCCTCAGAACGCGAAATGGTTCGCCATTATATCATTGAGTCG GTCAAATTCTGGGCGAAAGAATATCACATTGACGGTTTTAGATTCGATCTGATGGGGATACATGACATCGACACCATGAA CCAGTTAAGAGAGGAATTATTAAAAATTGACCCCACCATTTTTGTATACGGAGAAGGTTGGGTAGCAGCAGATTCTCCAT TACCTTTCGAGCAACGGGCTGTAAAGGAGAATGTACGAAAAATGAAAGGTATTTCCGTATTTAATGATGAATTTCGTGAC GGTCTGAAAGGAAGTACCTTTGACGAACAGGAACCAGGGTATGCCTCCGGAAATATCAACGGGCATTTTGAACCTGTCAA ATATGGAATCGTTGGCGGAACACAACACCCACAGGTAGATTATAGCGGGTTACTTTACTGTGACGGTCCATATGCCGCTG CTCCCTCTCAAATGATTAACTTTGTTTCCTGTCATGACGGATATACGCTGGTAGATAAGCTGAAATTATCTGTTCAAGGC GAACATTCTGAAGAAGAACTGGTTCCTATTGACAAATTAATACACACCGTGCTTTTAACCTCGCAAGGTATTCCTTTTAT TCGTGGCGGTGAAGAAATCATGCAGGACAAACAGGGAGAGCCAAACAGCTACAAGTCTTCTGACGCCGTCAACCAAATAG ACTGGGCGTTAAAAGCTAAAAACCGTGATATATTCAATTATATCAGAACATTAATCGCACTCCGCAAAAAACATCCCGCC TTCCGTATTCCTACAGCAGAGGGCTTAGAGAAATGGCTTCATTTTCTTGATACAGGCGATTCCGGTGTCATCGCTTATAC TCTAGGCGAGTATGCTAACGGTGATGAATGGAAAGAAATTCTGGTTGCATACAACGGTAACCGGAATCAGGCAGATATCA ATATCCCGGAACAAGATTGGATTATAGTCTGCCATAACGGACAAATTGATCTAGACAGTCAGGAGCATTTATCGGGAGGG GATATTTCTATCACAGCATCTTCAGCTCTTATCTTGTATCGTCAATAA
Upstream 100 bases:
>100_bases TCACATTTGTTTTCAGTCATACCATAACGGAATAATCCGGTTCATTTATTCCACAACATGATTATTTTTTTTATATAGTA TTAATATAAAATTAGAAAAT
Downstream 100 bases:
>100_bases AAAGGAGATCAACTTGATATTATTAAATAAAAGTACAATTATAAATCAGTTAGACTATGTTAGAAAATGAATTATGGGGA AATACTATTGAAAACTGGGG
Product: pullulanase
Products: NA
Alternate protein names: Alpha-dextrin endo-1,6-alpha-glucosidase; Pullulan 6-glucanohydrolase [H]
Number of amino acids: Translated: 655; Mature: 655
Protein sequence:
>655_residues MRELKNHPSPDQLDSYDKYPCYYENDLELVYTPQQSIFTLWAPSADNVRLNLYSSGEAGDPEEQLEMDIADNGTWCIHID RDLKGSFYTFQIEKDGKWLDETPGIWAKAVGINGNRAAVIDWNETNPEGWESDQSPELKMYSDIILYELHHRDFSIDPNS GIEHKGKFLALTETGTKTPEGESSGLDHLKELGVTHIHILPSFDFATIDERKLEKNKYNWGYDPKNYNVPDGSYSTDPVT PTTRIREFKEMVKSLHQNGFRIVLDVVYNHTASTEHSNFDLTVPGYFYRQNPDGSYSNASGCGNETASEREMVRHYIIES VKFWAKEYHIDGFRFDLMGIHDIDTMNQLREELLKIDPTIFVYGEGWVAADSPLPFEQRAVKENVRKMKGISVFNDEFRD GLKGSTFDEQEPGYASGNINGHFEPVKYGIVGGTQHPQVDYSGLLYCDGPYAAAPSQMINFVSCHDGYTLVDKLKLSVQG EHSEEELVPIDKLIHTVLLTSQGIPFIRGGEEIMQDKQGEPNSYKSSDAVNQIDWALKAKNRDIFNYIRTLIALRKKHPA FRIPTAEGLEKWLHFLDTGDSGVIAYTLGEYANGDEWKEILVAYNGNRNQADINIPEQDWIIVCHNGQIDLDSQEHLSGG DISITASSALILYRQ
Sequences:
>Translated_655_residues MRELKNHPSPDQLDSYDKYPCYYENDLELVYTPQQSIFTLWAPSADNVRLNLYSSGEAGDPEEQLEMDIADNGTWCIHID RDLKGSFYTFQIEKDGKWLDETPGIWAKAVGINGNRAAVIDWNETNPEGWESDQSPELKMYSDIILYELHHRDFSIDPNS GIEHKGKFLALTETGTKTPEGESSGLDHLKELGVTHIHILPSFDFATIDERKLEKNKYNWGYDPKNYNVPDGSYSTDPVT PTTRIREFKEMVKSLHQNGFRIVLDVVYNHTASTEHSNFDLTVPGYFYRQNPDGSYSNASGCGNETASEREMVRHYIIES VKFWAKEYHIDGFRFDLMGIHDIDTMNQLREELLKIDPTIFVYGEGWVAADSPLPFEQRAVKENVRKMKGISVFNDEFRD GLKGSTFDEQEPGYASGNINGHFEPVKYGIVGGTQHPQVDYSGLLYCDGPYAAAPSQMINFVSCHDGYTLVDKLKLSVQG EHSEEELVPIDKLIHTVLLTSQGIPFIRGGEEIMQDKQGEPNSYKSSDAVNQIDWALKAKNRDIFNYIRTLIALRKKHPA FRIPTAEGLEKWLHFLDTGDSGVIAYTLGEYANGDEWKEILVAYNGNRNQADINIPEQDWIIVCHNGQIDLDSQEHLSGG DISITASSALILYRQ >Mature_655_residues MRELKNHPSPDQLDSYDKYPCYYENDLELVYTPQQSIFTLWAPSADNVRLNLYSSGEAGDPEEQLEMDIADNGTWCIHID RDLKGSFYTFQIEKDGKWLDETPGIWAKAVGINGNRAAVIDWNETNPEGWESDQSPELKMYSDIILYELHHRDFSIDPNS GIEHKGKFLALTETGTKTPEGESSGLDHLKELGVTHIHILPSFDFATIDERKLEKNKYNWGYDPKNYNVPDGSYSTDPVT PTTRIREFKEMVKSLHQNGFRIVLDVVYNHTASTEHSNFDLTVPGYFYRQNPDGSYSNASGCGNETASEREMVRHYIIES VKFWAKEYHIDGFRFDLMGIHDIDTMNQLREELLKIDPTIFVYGEGWVAADSPLPFEQRAVKENVRKMKGISVFNDEFRD GLKGSTFDEQEPGYASGNINGHFEPVKYGIVGGTQHPQVDYSGLLYCDGPYAAAPSQMINFVSCHDGYTLVDKLKLSVQG EHSEEELVPIDKLIHTVLLTSQGIPFIRGGEEIMQDKQGEPNSYKSSDAVNQIDWALKAKNRDIFNYIRTLIALRKKHPA FRIPTAEGLEKWLHFLDTGDSGVIAYTLGEYANGDEWKEILVAYNGNRNQADINIPEQDWIIVCHNGQIDLDSQEHLSGG DISITASSALILYRQ
Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]
COG id: COG1523
COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the glycosyl hydrolase 13 family [H]
Homologues:
Organism=Escherichia coli, GI2367229, Length=546, Percent_Identity=24.9084249084249, Blast_Score=134, Evalue=2e-32,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR006047 - InterPro: IPR004193 - InterPro: IPR017853 - InterPro: IPR013781 - InterPro: IPR013783 - InterPro: IPR014756 - InterPro: IPR005323 - InterPro: IPR011840 [H]
Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48; PF03714 PUD [H]
EC number: =3.2.1.41 [H]
Molecular weight: Translated: 74319; Mature: 74319
Theoretical pI: Translated: 4.60; Mature: 4.60
Prosite motif: PS00228 TUBULIN_B_AUTOREG
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.9 %Cys (Translated Protein) 1.5 %Met (Translated Protein) 2.4 %Cys+Met (Translated Protein) 0.9 %Cys (Mature Protein) 1.5 %Met (Mature Protein) 2.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MRELKNHPSPDQLDSYDKYPCYYENDLELVYTPQQSIFTLWAPSADNVRLNLYSSGEAGD CCCCCCCCCCCCCCCCCCCCCEECCCCEEEECCCCCEEEEECCCCCCEEEEEEECCCCCC PEEQLEMDIADNGTWCIHIDRDLKGSFYTFQIEKDGKWLDETPGIWAKAVGINGNRAAVI CHHHEEEEECCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEE DWNETNPEGWESDQSPELKMYSDIILYELHHRDFSIDPNSGIEHKGKFLALTETGTKTPE ECCCCCCCCCCCCCCCCCEEHHEEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCC GESSGLDHLKELGVTHIHILPSFDFATIDERKLEKNKYNWGYDPKNYNVPDGSYSTDPVT CCCCCHHHHHHCCCEEEEEEECCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC PTTRIREFKEMVKSLHQNGFRIVLDVVYNHTASTEHSNFDLTVPGYFYRQNPDGSYSNAS CHHHHHHHHHHHHHHHCCCCEEEEEEHHCCCCCCCCCCEEEECCCEEEEECCCCCCCCCC GCGNETASEREMVRHYIIESVKFWAKEYHIDGFRFDLMGIHDIDTMNQLREELLKIDPTI CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHHHHHCCCEE FVYGEGWVAADSPLPFEQRAVKENVRKMKGISVFNDEFRDGLKGSTFDEQEPGYASGNIN EEEECCEEECCCCCCHHHHHHHHHHHHHCCCCEECHHHHCCCCCCCCCCCCCCEECCCCC GHFEPVKYGIVGGTQHPQVDYSGLLYCDGPYAAAPSQMINFVSCHDGYTLVDKLKLSVQG CCCCEEEEEEECCCCCCCCCCCEEEEECCCCCCCHHHHHEEEEECCCCEEEEEEEEEECC EHSEEELVPIDKLIHTVLLTSQGIPFIRGGEEIMQDKQGEPNSYKSSDAVNQIDWALKAK CCCCCCCCCHHHHHHHHHHHCCCCCEEECCHHHHHCCCCCCCCCCCCCHHHHHHHEEECC NRDIFNYIRTLIALRKKHPAFRIPTAEGLEKWLHFLDTGDSGVIAYTLGEYANGDEWKEI CCHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEE LVAYNGNRNQADINIPEQDWIIVCHNGQIDLDSQEHLSGGDISITASSALILYRQ EEEECCCCCEEEECCCCCCEEEEEECCEECCCCHHCCCCCCEEEEECCEEEEEEC >Mature Secondary Structure MRELKNHPSPDQLDSYDKYPCYYENDLELVYTPQQSIFTLWAPSADNVRLNLYSSGEAGD CCCCCCCCCCCCCCCCCCCCCEECCCCEEEECCCCCEEEEECCCCCCEEEEEEECCCCCC PEEQLEMDIADNGTWCIHIDRDLKGSFYTFQIEKDGKWLDETPGIWAKAVGINGNRAAVI CHHHEEEEECCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEE DWNETNPEGWESDQSPELKMYSDIILYELHHRDFSIDPNSGIEHKGKFLALTETGTKTPE ECCCCCCCCCCCCCCCCCEEHHEEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCC GESSGLDHLKELGVTHIHILPSFDFATIDERKLEKNKYNWGYDPKNYNVPDGSYSTDPVT CCCCCHHHHHHCCCEEEEEEECCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC PTTRIREFKEMVKSLHQNGFRIVLDVVYNHTASTEHSNFDLTVPGYFYRQNPDGSYSNAS CHHHHHHHHHHHHHHHCCCCEEEEEEHHCCCCCCCCCCEEEECCCEEEEECCCCCCCCCC GCGNETASEREMVRHYIIESVKFWAKEYHIDGFRFDLMGIHDIDTMNQLREELLKIDPTI CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHHHHHCCCEE FVYGEGWVAADSPLPFEQRAVKENVRKMKGISVFNDEFRDGLKGSTFDEQEPGYASGNIN EEEECCEEECCCCCCHHHHHHHHHHHHHCCCCEECHHHHCCCCCCCCCCCCCCEECCCCC GHFEPVKYGIVGGTQHPQVDYSGLLYCDGPYAAAPSQMINFVSCHDGYTLVDKLKLSVQG CCCCEEEEEEECCCCCCCCCCCEEEEECCCCCCCHHHHHEEEEECCCCEEEEEEEEEECC EHSEEELVPIDKLIHTVLLTSQGIPFIRGGEEIMQDKQGEPNSYKSSDAVNQIDWALKAK CCCCCCCCCHHHHHHHHHHHCCCCCEEECCHHHHHCCCCCCCCCCCCCHHHHHHHEEECC NRDIFNYIRTLIALRKKHPAFRIPTAEGLEKWLHFLDTGDSGVIAYTLGEYANGDEWKEI CCHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEE LVAYNGNRNQADINIPEQDWIIVCHNGQIDLDSQEHLSGGDISITASSALILYRQ EEEECCCCCEEEECCCCCCEEEEEECCEECCCCHHCCCCCCEEEEECCEEEEEEC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9453151; 10360571 [H]