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The map label for this gene is recA [H]

Identifier: 29350018

GI number: 29350018

Start: 6035657

End: 6036691

Strand: Reverse

Name: recA [H]

Synonym: BT_4610

Alternate gene names: 29350018

Gene position: 6036691-6035657 (Counterclockwise)

Preceding gene: 29350019

Following gene: 29350009

Centisome position: 96.43

GC content: 43.29

Gene sequence:

>1035_bases
ATGGCAAAGAAAGACGAACTTAATTTTGAAACAGATAATAAAATGGCATCAAGCGAAAAACTGAAAGCCTTACAGGCTAC
CATGGAAAAGATAGAAAAGAACTTCGGTAAAGGTTCTATCATGAAAATGGGTGATGATAGTGTTGAACAAGTGGAAGTGA
TTCCTACCGGCTCCATTGCTTTAAACGTGGCACTTGGCGTAGGAGGATATCCTAGGGGAAGAATCATCGAAATCTATGGT
CCGGAATCTTCCGGTAAAACGACTCTGGCTATTCACGCCATTGCCGAAGCACAAAAAGCCGGTGGTATCGCCGCTTTTAT
TGATGCCGAACATGCCTTCGACCGTTTTTATGCAGCGAAATTAGGCGTGGATGTAGACAATCTCTGGATTTCTCAACCGG
ACAACGGAGAGCAGGCCTTGGAAATAGCCGAACAGTTAATCCGTTCTTCCGCTATTGATATTATTGTTATTGACTCCGTA
GCAGCATTAACTCCGAAAGCAGAAATTGAAGGCGACATGGGTGACAATAAGGTAGGTTTGCAGGCACGTCTGATGTCACA
GGCTTTGCGTAAATTGACCGGTACAGTCAGCAAAACACGTACAACTTGTATCTTTATCAACCAGCTCCGTGAAAAGATTG
GCGTCATGTTCGGTAATCCGGAAACAACGACAGGTGGTAACGCATTGAAGTTCTATGCTTCTGTACGTATAGATATCCGT
GGCAGCCAGCCAATCAAAGACGGTGAAGAAGTTCTTGGTAAACTGACTAAGGTGAAAGTGGTGAAGAACAAAGTAGCACC
TCCTTTCCGCAAAGCAGAATTTGATATCATGTTTGGTGAAGGTATTTCTCACTCCGGAGAAATCATCGATTTGGGCGCAG
AACTGGGAATCATCAAAAAGAGCGGTTCATGGTATAGCTACAATGACACTAAACTGGGTCAAGGACGTGATGCAGCCAAG
GTATGTATCAAAGATAATCCCGAACTTGCTGAAGAATTAGAAGGATTGATCTTCGAAGCCTTGAATAAAAAATAA

Upstream 100 bases:

>100_bases
AGGAATCATAGAACGGATTATCACTCCCAAAGAAGTGAAAACCAAAGAGCATGCCTCACAGATTTTAAATCAATAAATCC
ATTAATTATAAGAATAAGGT

Downstream 100 bases:

>100_bases
GTTGTCTCAACCGATTCAACTATAAATAATATAAGCTGTCTCATTCGATGTTTTGAGACAGCTTATTTTTAGCTTAAACA
TAAGAAATGTCCCCCATCTG

Product: recombinase A

Products: NA

Alternate protein names: Recombinase A [H]

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MAKKDELNFETDNKMASSEKLKALQATMEKIEKNFGKGSIMKMGDDSVEQVEVIPTGSIALNVALGVGGYPRGRIIEIYG
PESSGKTTLAIHAIAEAQKAGGIAAFIDAEHAFDRFYAAKLGVDVDNLWISQPDNGEQALEIAEQLIRSSAIDIIVIDSV
AALTPKAEIEGDMGDNKVGLQARLMSQALRKLTGTVSKTRTTCIFINQLREKIGVMFGNPETTTGGNALKFYASVRIDIR
GSQPIKDGEEVLGKLTKVKVVKNKVAPPFRKAEFDIMFGEGISHSGEIIDLGAELGIIKKSGSWYSYNDTKLGQGRDAAK
VCIKDNPELAEELEGLIFEALNKK

Sequences:

>Translated_344_residues
MAKKDELNFETDNKMASSEKLKALQATMEKIEKNFGKGSIMKMGDDSVEQVEVIPTGSIALNVALGVGGYPRGRIIEIYG
PESSGKTTLAIHAIAEAQKAGGIAAFIDAEHAFDRFYAAKLGVDVDNLWISQPDNGEQALEIAEQLIRSSAIDIIVIDSV
AALTPKAEIEGDMGDNKVGLQARLMSQALRKLTGTVSKTRTTCIFINQLREKIGVMFGNPETTTGGNALKFYASVRIDIR
GSQPIKDGEEVLGKLTKVKVVKNKVAPPFRKAEFDIMFGEGISHSGEIIDLGAELGIIKKSGSWYSYNDTKLGQGRDAAK
VCIKDNPELAEELEGLIFEALNKK
>Mature_343_residues
AKKDELNFETDNKMASSEKLKALQATMEKIEKNFGKGSIMKMGDDSVEQVEVIPTGSIALNVALGVGGYPRGRIIEIYGP
ESSGKTTLAIHAIAEAQKAGGIAAFIDAEHAFDRFYAAKLGVDVDNLWISQPDNGEQALEIAEQLIRSSAIDIIVIDSVA
ALTPKAEIEGDMGDNKVGLQARLMSQALRKLTGTVSKTRTTCIFINQLREKIGVMFGNPETTTGGNALKFYASVRIDIRG
SQPIKDGEEVLGKLTKVKVVKNKVAPPFRKAEFDIMFGEGISHSGEIIDLGAELGIIKKSGSWYSYNDTKLGQGRDAAKV
CIKDNPELAEELEGLIFEALNKK

Specific function: Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation

COG id: COG0468

COG function: function code L; RecA/RadA recombinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family [H]

Homologues:

Organism=Escherichia coli, GI1789051, Length=322, Percent_Identity=62.7329192546584, Blast_Score=418, Evalue=1e-118,

Paralogues:

None

Copy number: 1548 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800-1200 (L-broth) 40,000-60,000 (L-broth + Nalidixate) 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013765
- InterPro:   IPR020584
- InterPro:   IPR020588
- InterPro:   IPR020587 [H]

Pfam domain/function: PF00154 RecA [H]

EC number: NA

Molecular weight: Translated: 37210; Mature: 37079

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00321 RECA_1 ; PS50162 RECA_2 ; PS50163 RECA_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKDELNFETDNKMASSEKLKALQATMEKIEKNFGKGSIMKMGDDSVEQVEVIPTGSIA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCEEEEEECCCEE
LNVALGVGGYPRGRIIEIYGPESSGKTTLAIHAIAEAQKAGGIAAFIDAEHAFDRFYAAK
EEEEEECCCCCCCCEEEEECCCCCCCEEEEEEEEHHHHHHCCEEEEEECHHHHHHHHHHH
LGVDVDNLWISQPDNGEQALEIAEQLIRSSAIDIIVIDSVAALTPKAEIEGDMGDNKVGL
HCCCHHHEEECCCCCHHHHHHHHHHHHHHCCCCEEEECCHHHCCCCHHCCCCCCCCCHHH
QARLMSQALRKLTGTVSKTRTTCIFINQLREKIGVMFGNPETTTGGNALKFYASVRIDIR
HHHHHHHHHHHHHCCHHHHHHHEEEHHHHHHHHCEEECCCCCCCCCCEEEEEEEEEEEEC
GSQPIKDGEEVLGKLTKVKVVKNKVAPPFRKAEFDIMFGEGISHSGEIIDLGAELGIIKK
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEEECCCEEEEEE
SGSWYSYNDTKLGQGRDAAKVCIKDNPELAEELEGLIFEALNKK
CCCEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKKDELNFETDNKMASSEKLKALQATMEKIEKNFGKGSIMKMGDDSVEQVEVIPTGSIA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCEEEEEECCCEE
LNVALGVGGYPRGRIIEIYGPESSGKTTLAIHAIAEAQKAGGIAAFIDAEHAFDRFYAAK
EEEEEECCCCCCCCEEEEECCCCCCCEEEEEEEEHHHHHHCCEEEEEECHHHHHHHHHHH
LGVDVDNLWISQPDNGEQALEIAEQLIRSSAIDIIVIDSVAALTPKAEIEGDMGDNKVGL
HCCCHHHEEECCCCCHHHHHHHHHHHHHHCCCCEEEECCHHHCCCCHHCCCCCCCCCHHH
QARLMSQALRKLTGTVSKTRTTCIFINQLREKIGVMFGNPETTTGGNALKFYASVRIDIR
HHHHHHHHHHHHHCCHHHHHHHEEEHHHHHHHHCEEECCCCCCCCCCEEEEEEEEEEEEC
GSQPIKDGEEVLGKLTKVKVVKNKVAPPFRKAEFDIMFGEGISHSGEIIDLGAELGIIKK
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEEECCCEEEEEE
SGSWYSYNDTKLGQGRDAAKVCIKDNPELAEELEGLIFEALNKK
CCCEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928; 9335266 [H]