Click here to switch to the map view.

The map label for this gene is eno [H]

Identifier: 29349980

GI number: 29349980

Start: 5993128

End: 5994408

Strand: Reverse

Name: eno [H]

Synonym: BT_4572

Alternate gene names: 29349980

Gene position: 5994408-5993128 (Counterclockwise)

Preceding gene: 29349981

Following gene: 29349979

Centisome position: 95.75

GC content: 48.09

Gene sequence:

>1281_bases
ATGAAAATAGAAAAAATTGTAGCTCGAGAAATTCTCGATTCAAGAGGTAACCCCACAGTAGAAGTTGACGTAGTATTGGA
ATCAGGTATCATGGGACGTGCGTCTGTTCCGTCAGGTGCTTCCACAGGTGAACATGAAGCACTCGAACTTCGTGATGGTG
ACAAGCAACGTTACGGTGGCAAAGGCGTACAAAAGGCGGTGGACAATGTAAACAAGATCATTGCTCCGAAACTGATCGGT
ATGTCTTCTCTCAACCAAAGAGGAATCGACTACGCAATGTTGGCACTCGACGGTACTAAAACCAAGTCCAATCTAGGTGC
TAACGCTATTCTTGGCGTATCTCTCGCTGTAGCCAAAGCAGCAGCCAGCTATCTTGATCTCCCTCTCTATCGCTATATCG
GCGGAACAAATACATACGTAATGCCTGTACCGATGATGAATATCATCAATGGCGGTTCACACAGTGACGCTCCTATCGCA
TTCCAGGAATTCATGATTCGTCCGGTAGGTGCACCCTCATTCAGAGAAGGTTTGAGAATGGGCGCCGAAGTATTCCACGC
TTTGAAGAAAGTACTGAAAGATCGTGGCCTCAGCACTGCCGTAGGCGACGAAGGTGGTTTCGCTCCTAACCTCGAAGGTA
CGGAAGATGCTCTGAACTCTATCATCGCAGCCATCAAAGCTGCCGGATACGAACCAGGTAAAGACGTAATGATCGGTATG
GACTGCGCTTCTTCCGAATTCTACCATGACGGTATCTACGACTATACCAAGTTTGAAGGTGCCAAAGGCAAGAAACGTAC
CGCCGAAGAACAGATCGACTACCTGGAAGAACTGATCAACAAATTCCCAATCGACTCCATCGAAGACGGTATGAGCGAAA
ACGACTGGGAAGGCTGGAAGAAACTGACTGAACGTATCGGCGACCGCTGCCAGTTGGTAGGTGATGACCTGTTCGTTACG
AACGTTGACTTCCTCGCAATGGGTATCGAGAAGGGATGTGCAAACTCTATCCTGATCAAAGTAAACCAAATCGGTTCGCT
GACCGAAACTCTGAACGCTATCGAAATGGCTCACCGTCATGGCTATACGACTGTCACTTCCCACCGCTCCGGCGAAACGG
AAGACGCAACGATTGCAGACATCGCAGTAGCTACGAATAGCGGACAGATCAAGACCGGTTCATTAAGCCGTTCGGACCGT
ATGGCTAAATATAACCAACTGCTCCGCATCGAAGAAGAACTCGGTGACTTGGCTGTATACGGATATAAGAGAATCAAATA
A

Upstream 100 bases:

>100_bases
TCTAGGGAGTATGATTCGAAAGAATACCTATTTATAGTGATTTTCCACTCTCTGTCAAGTCCGTACTTTTGCAAAAATAT
TATTCAAAACAATAGAAACG

Downstream 100 bases:

>100_bases
TCTTTATTCTCTTTTCAGATATCTTTCCCCCGGGCAATGTGTTGTCCGGGGATTTTTTTATATATTTGATGACTTACAAA
CGACCTGAAGCATTTTGCTA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MKIEKIVAREILDSRGNPTVEVDVVLESGIMGRASVPSGASTGEHEALELRDGDKQRYGGKGVQKAVDNVNKIIAPKLIG
MSSLNQRGIDYAMLALDGTKTKSNLGANAILGVSLAVAKAAASYLDLPLYRYIGGTNTYVMPVPMMNIINGGSHSDAPIA
FQEFMIRPVGAPSFREGLRMGAEVFHALKKVLKDRGLSTAVGDEGGFAPNLEGTEDALNSIIAAIKAAGYEPGKDVMIGM
DCASSEFYHDGIYDYTKFEGAKGKKRTAEEQIDYLEELINKFPIDSIEDGMSENDWEGWKKLTERIGDRCQLVGDDLFVT
NVDFLAMGIEKGCANSILIKVNQIGSLTETLNAIEMAHRHGYTTVTSHRSGETEDATIADIAVATNSGQIKTGSLSRSDR
MAKYNQLLRIEEELGDLAVYGYKRIK

Sequences:

>Translated_426_residues
MKIEKIVAREILDSRGNPTVEVDVVLESGIMGRASVPSGASTGEHEALELRDGDKQRYGGKGVQKAVDNVNKIIAPKLIG
MSSLNQRGIDYAMLALDGTKTKSNLGANAILGVSLAVAKAAASYLDLPLYRYIGGTNTYVMPVPMMNIINGGSHSDAPIA
FQEFMIRPVGAPSFREGLRMGAEVFHALKKVLKDRGLSTAVGDEGGFAPNLEGTEDALNSIIAAIKAAGYEPGKDVMIGM
DCASSEFYHDGIYDYTKFEGAKGKKRTAEEQIDYLEELINKFPIDSIEDGMSENDWEGWKKLTERIGDRCQLVGDDLFVT
NVDFLAMGIEKGCANSILIKVNQIGSLTETLNAIEMAHRHGYTTVTSHRSGETEDATIADIAVATNSGQIKTGSLSRSDR
MAKYNQLLRIEEELGDLAVYGYKRIK
>Mature_426_residues
MKIEKIVAREILDSRGNPTVEVDVVLESGIMGRASVPSGASTGEHEALELRDGDKQRYGGKGVQKAVDNVNKIIAPKLIG
MSSLNQRGIDYAMLALDGTKTKSNLGANAILGVSLAVAKAAASYLDLPLYRYIGGTNTYVMPVPMMNIINGGSHSDAPIA
FQEFMIRPVGAPSFREGLRMGAEVFHALKKVLKDRGLSTAVGDEGGFAPNLEGTEDALNSIIAAIKAAGYEPGKDVMIGM
DCASSEFYHDGIYDYTKFEGAKGKKRTAEEQIDYLEELINKFPIDSIEDGMSENDWEGWKKLTERIGDRCQLVGDDLFVT
NVDFLAMGIEKGCANSILIKVNQIGSLTETLNAIEMAHRHGYTTVTSHRSGETEDATIADIAVATNSGQIKTGSLSRSDR
MAKYNQLLRIEEELGDLAVYGYKRIK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=428, Percent_Identity=54.2056074766355, Blast_Score=436, Evalue=1e-122,
Organism=Homo sapiens, GI301897477, Length=435, Percent_Identity=52.183908045977, Blast_Score=417, Evalue=1e-116,
Organism=Homo sapiens, GI301897469, Length=435, Percent_Identity=52.183908045977, Blast_Score=417, Evalue=1e-116,
Organism=Homo sapiens, GI4503571, Length=435, Percent_Identity=51.2643678160919, Blast_Score=411, Evalue=1e-115,
Organism=Homo sapiens, GI301897479, Length=433, Percent_Identity=48.0369515011547, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI169201331, Length=347, Percent_Identity=27.6657060518732, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI169201757, Length=347, Percent_Identity=27.6657060518732, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI239744207, Length=347, Percent_Identity=27.6657060518732, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI1789141, Length=430, Percent_Identity=63.0232558139535, Blast_Score=506, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI17536383, Length=429, Percent_Identity=55.4778554778555, Blast_Score=426, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI71995829, Length=429, Percent_Identity=55.4778554778555, Blast_Score=426, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI32563855, Length=187, Percent_Identity=48.6631016042781, Blast_Score=172, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6321693, Length=431, Percent_Identity=51.0440835266821, Blast_Score=412, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6324974, Length=436, Percent_Identity=50.2293577981651, Blast_Score=409, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6324969, Length=436, Percent_Identity=50.2293577981651, Blast_Score=409, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6323985, Length=436, Percent_Identity=50.2293577981651, Blast_Score=409, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6321968, Length=432, Percent_Identity=51.1574074074074, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580918, Length=424, Percent_Identity=53.3018867924528, Blast_Score=403, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580916, Length=424, Percent_Identity=53.3018867924528, Blast_Score=403, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580920, Length=424, Percent_Identity=53.3018867924528, Blast_Score=403, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580914, Length=424, Percent_Identity=53.3018867924528, Blast_Score=403, Evalue=1e-112,
Organism=Drosophila melanogaster, GI281360527, Length=424, Percent_Identity=53.3018867924528, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI17137654, Length=424, Percent_Identity=53.3018867924528, Blast_Score=402, Evalue=1e-112,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 46159; Mature: 46159

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIEKIVAREILDSRGNPTVEVDVVLESGIMGRASVPSGASTGEHEALELRDGDKQRYGG
CCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEECCCCCHHHCCC
KGVQKAVDNVNKIIAPKLIGMSSLNQRGIDYAMLALDGTKTKSNLGANAILGVSLAVAKA
CHHHHHHHHHHHHHHHHHHCHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
AASYLDLPLYRYIGGTNTYVMPVPMMNIINGGSHSDAPIAFQEFMIRPVGAPSFREGLRM
HHHHHCCHHHHHHCCCCCEEECCCHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHH
GAEVFHALKKVLKDRGLSTAVGDEGGFAPNLEGTEDALNSIIAAIKAAGYEPGKDVMIGM
HHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEC
DCASSEFYHDGIYDYTKFEGAKGKKRTAEEQIDYLEELINKFPIDSIEDGMSENDWEGWK
CCCCCCHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHH
KLTERIGDRCQLVGDDLFVTNVDFLAMGIEKGCANSILIKVNQIGSLTETLNAIEMAHRH
HHHHHHCCHHEEECCCEEEECHHHHHHHHHHCCCCCEEEEEHHHCCHHHHHHHHHHHHHC
GYTTVTSHRSGETEDATIADIAVATNSGQIKTGSLSRSDRMAKYNQLLRIEEELGDLAVY
CCCEEECCCCCCCCCCEEEEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHCCEEEC
GYKRIK
CHHCCC
>Mature Secondary Structure
MKIEKIVAREILDSRGNPTVEVDVVLESGIMGRASVPSGASTGEHEALELRDGDKQRYGG
CCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEECCCCCHHHCCC
KGVQKAVDNVNKIIAPKLIGMSSLNQRGIDYAMLALDGTKTKSNLGANAILGVSLAVAKA
CHHHHHHHHHHHHHHHHHHCHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
AASYLDLPLYRYIGGTNTYVMPVPMMNIINGGSHSDAPIAFQEFMIRPVGAPSFREGLRM
HHHHHCCHHHHHHCCCCCEEECCCHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHH
GAEVFHALKKVLKDRGLSTAVGDEGGFAPNLEGTEDALNSIIAAIKAAGYEPGKDVMIGM
HHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEC
DCASSEFYHDGIYDYTKFEGAKGKKRTAEEQIDYLEELINKFPIDSIEDGMSENDWEGWK
CCCCCCHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHH
KLTERIGDRCQLVGDDLFVTNVDFLAMGIEKGCANSILIKVNQIGSLTETLNAIEMAHRH
HHHHHHCCHHEEECCCEEEECHHHHHHHHHHCCCCCEEEEEHHHCCHHHHHHHHHHHHHC
GYTTVTSHRSGETEDATIADIAVATNSGQIKTGSLSRSDRMAKYNQLLRIEEELGDLAVY
CCCEEECCCCCCCCCCEEEEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHCCEEEC
GYKRIK
CHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA