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The map label for this gene is algU [H]

Identifier: 29349979

GI number: 29349979

Start: 5992436

End: 5992996

Strand: Reverse

Name: algU [H]

Synonym: BT_4571

Alternate gene names: 29349979

Gene position: 5992996-5992436 (Counterclockwise)

Preceding gene: 29349980

Following gene: 29349978

Centisome position: 95.73

GC content: 42.78

Gene sequence:

>561_bases
ATGAATCAGGAAAAAATACAGGAACTGGTCAGCCGAAGTCAGCAGGACGACAAAAGGGCCTTCGCCCTACTGGTTTCAGA
ATTCCAGCCACTGGTATTCCGACTAGCTTTCCGTTTACTTTGTGATGAAGAGGAAGCCAGAGACATAACACAAGAAACAT
TTGTAAAAGTATGGTTATCCCTGAAAACGTACAATCAGGAAAACCGCCTGTCAACCTGGCTTTACAAAATCACCTGTAAC
ACCTGTTACGACCGTTTGCGTTCGTTACGCCATTCTCCGCTGGATAATGAATCCGCCTTTTCCGATTCGGTAAATATTCC
TTCTGACGATCATATTGAGATATCTCTTTCCAACAAGCAGTTGAAAGAACTGATATTGCGATATACCAACGAACTGCCTC
CCCAGCAGAAACTCGTTTTCACGCTTCGGGATGTCGAAGAGTTGGAGGTAGCAGAAGTACAGATTATCACAGGATTATCG
CCCGAAAAGATAAAAAGTACCTTGTACCTTGCCAGAAAGAACATACGTAATAAAATGAATCAAATAGATCCCGACTTATG
A

Upstream 100 bases:

>100_bases
GGGCAATGTGTTGTCCGGGGATTTTTTTATATATTTGATGACTTACAAACGACCTGAAGCATTTTGCTACGTATCATATA
GTAAACAGGAATAAATAATG

Downstream 100 bases:

>100_bases
AACAAGAAGATAAACAGTATGAAAAATGGCTGACCGAAGTCAAAAGTAACCAGCCGATATTGGAAAATCCGGAGGAACTG
ACCGCTACCATCCTCAACAG

Product: RNA polymerase ECF-type sigma factor

Products: NA

Alternate protein names: Sigma-30 [H]

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MNQEKIQELVSRSQQDDKRAFALLVSEFQPLVFRLAFRLLCDEEEARDITQETFVKVWLSLKTYNQENRLSTWLYKITCN
TCYDRLRSLRHSPLDNESAFSDSVNIPSDDHIEISLSNKQLKELILRYTNELPPQQKLVFTLRDVEELEVAEVQIITGLS
PEKIKSTLYLARKNIRNKMNQIDPDL

Sequences:

>Translated_186_residues
MNQEKIQELVSRSQQDDKRAFALLVSEFQPLVFRLAFRLLCDEEEARDITQETFVKVWLSLKTYNQENRLSTWLYKITCN
TCYDRLRSLRHSPLDNESAFSDSVNIPSDDHIEISLSNKQLKELILRYTNELPPQQKLVFTLRDVEELEVAEVQIITGLS
PEKIKSTLYLARKNIRNKMNQIDPDL
>Mature_186_residues
MNQEKIQELVSRSQQDDKRAFALLVSEFQPLVFRLAFRLLCDEEEARDITQETFVKVWLSLKTYNQENRLSTWLYKITCN
TCYDRLRSLRHSPLDNESAFSDSVNIPSDDHIEISLSNKQLKELILRYTNELPPQQKLVFTLRDVEELEVAEVQIITGLS
PEKIKSTLYLARKNIRNKMNQIDPDL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates genes such as algD, involved in alginate biosynthesis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=179, Percent_Identity=28.4916201117318, Blast_Score=79, Evalue=1e-16,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 21863; Mature: 21863

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQEKIQELVSRSQQDDKRAFALLVSEFQPLVFRLAFRLLCDEEEARDITQETFVKVWLS
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LKTYNQENRLSTWLYKITCNTCYDRLRSLRHSPLDNESAFSDSVNIPSDDHIEISLSNKQ
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECHHH
LKELILRYTNELPPQQKLVFTLRDVEELEVAEVQIITGLSPEKIKSTLYLARKNIRNKMN
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHH
QIDPDL
HCCCCC
>Mature Secondary Structure
MNQEKIQELVSRSQQDDKRAFALLVSEFQPLVFRLAFRLLCDEEEARDITQETFVKVWLS
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LKTYNQENRLSTWLYKITCNTCYDRLRSLRHSPLDNESAFSDSVNIPSDDHIEISLSNKQ
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECHHH
LKELILRYTNELPPQQKLVFTLRDVEELEVAEVQIITGLSPEKIKSTLYLARKNIRNKMN
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHH
QIDPDL
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432708; 8378309; 7961421; 10984043; 7737518 [H]