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The map label for this gene is dnaG [H]

Identifier: 29349340

GI number: 29349340

Start: 5104729

End: 5106921

Strand: Reverse

Name: dnaG [H]

Synonym: BT_3932

Alternate gene names: 29349340

Gene position: 5106921-5104729 (Counterclockwise)

Preceding gene: 29349341

Following gene: 29349325

Centisome position: 81.58

GC content: 49.11

Gene sequence:

>2193_bases
ATGATAGATCAAGTTACCATAGACCGAATTTTAGATGCCGCACAGATTGTAGATGTAGTGTCTGAGTTTGTCACCCTGCG
CAAACGCGGAGTGAACTATGTAGGTCTGTGCCCGTTCCACAACGAAAAGACTCCGTCATTCAGCGTCTCCCCTGCCAAAG
GACTTTGCAAGTGTTTCAGCTGCGGTAAGGGCGGAAACTCGGTCCACTTCATCATGGAGCACGAACAGATGTCGTATTAC
GAGGCACTGAAATATCTCGCCAAGAAATACAACATCGAAATCAAAGAACGGGAGCTGACCAATGAAGAGAAACAGGCGCA
GACAACGCGCGAAAGTATGTTCATTGTCAACAACTTCGCCCGCGATTACTTTCAGAATATCTTAAAGAATCATGTGGACG
GGCGCAGCATCGGTCTGGCTTATTTCCGTCAGCGTGGATTTCGTGATGACATCATTGAGAAATTCCAGTTGGGTTACTGT
ACGGAAAGCCATGATGCCATGTCACAAGAAGCATTGCGCAAAGGCTACAAGAAAGAATTCCTGGTGAAAACGGGAATCTG
CTACGAAACGGATGACCACCGTCTTCGTGACCGCTTCTGGGGACGTGTGATTTTCCCCGTCCATACCCTTTCCGGAAAGG
TGGTCGCCTTCGGCGGACGTGTGCTCAGCACCGCCACCAAAGGGGTAAAGGTGAAATATGTCAACTCTCCCGAGTCGGAA
ATTTACCACAAGAGTAACGAGCTATACGGAATCTATTTTGCCAAACAGGCGATTGTCAGGCAGGACAGATGTTTCCTTGT
GGAAGGATACACGGACGTTATCTCCATGCACCAGTCGGGCATAGAAAACGTAGTCGCTTCATCGGGCACCGCATTGACGC
CGGGACAGATTCGTCTGATCCACCGTTTCACCAATAACATTACCGTACTCTATGACGGTGACGTAGCCGGTATCAAAGCC
TCTATCCGCGGAATCGATATGCTGCTGGAAGAAGGCATGAACATCAAAGTATGCCTGCTTCCGGACGGAGACGACCCGGA
CTCCTTCGCCCGCAAACATAATTCGGAAGAATTCCAGGCATTCATCCGTGAGCACGAGAAAGATTTCATCCGCTTCAAGA
CCGACCTTCTGATGGAAGACGCGGGAAGAGATCCGATCAAACGCGCGGAACTGATTTCGAATATCGTACGAAGCATTTCC
GTCATACCGGAAGCCATTATACGGGATGTCTACATCAAAGAATGCAGCCAACATCTGCGCATCGAAGAAAAACTGCTGGT
AGCGGAAGTCGCCAAATTAAGAGAGGCGCAGGCTGAAAAAGCCAATCGTCCCAGCTATAATCATTCATCTTCAACAACCG
GAGATACTTCCGGTACAGCTGCCGCTTCCGGCACACCGGCTTATTCCGGTGCTCCGACTTATTCTACTCCATCAAGTCCC
TACGCCAATGCAGGAATGCCACCGGAACCCGAATATGATGACGATGGCAATATCGTATCTTTCGCAGATCCGATGTCCGC
TGCTACAGGAGGTGCAGCAGGATTTCCTCCCGGCAATGCACCTGCCTCCACTACGGATACCACTGTTCCCGGAGATTCTT
ATACCTCCTTTATTCCGCAGGAAGGAAAAGAAGGTCAGGAGTTCTATAAGTTTGAGCGATTGATCCTGCAGGCAGTAGTC
CGTTATGGTGAAAAAGTGATGTGCAACCTTACCGACGAAGAAGGAAATGAAATTCCGGTAACCGTTATCGAATATGTGGT
GAATGATCTGAAGGAAGACGACCTGGCTTTCCATAATCCGTTGCACCGACAGATTCTGACAGAAGCCGCCACGCATATAC
ACGATGCCGGTTTTATCGCGGAACGCTATTTCCTGGCACATCCCGACCAGACCATCAGCCGGTTGAGCGTGGACTTGATC
AATGTACGTTATCAGCTAAGTAAATATCACTCGAAATCACAGAAGATCGTGACAGATGAAGAACGGCTGTACGAACTCGT
CCCTATGTTGATGATCAACTTCAAGTATGCGATTGTCACGGAAGAACTGAAACATATGCTTTATGCCCTGCAAGATCCGG
CACTCGCTCATGACAATGAGAAATGTAATTCGCTGATGCAGCGTTATAATGAGATGCGGGAAGTGCAAAGTCTGATGGCG
AAGCGCCTGGGCGACCGCGTTGTATTACGCTAA

Upstream 100 bases:

>100_bases
CGATAAGATTTAACCATCCCCTGCAAGAAAGTTCTTTGCAGGGGATTTTTTTATATGTACCTTTGTATCCGCAAAAACGG
AATCACAGGAGAAAAGAAGA

Downstream 100 bases:

>100_bases
ATTTCTCATCGGTGCCGAGACACCCCTTCATCGGTACCGGAGGCAATCCTCATTGACGGGGAACTACATCGTCCACATTT
TGTAAAGCGTATTACACCCT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 730; Mature: 730

Protein sequence:

>730_residues
MIDQVTIDRILDAAQIVDVVSEFVTLRKRGVNYVGLCPFHNEKTPSFSVSPAKGLCKCFSCGKGGNSVHFIMEHEQMSYY
EALKYLAKKYNIEIKERELTNEEKQAQTTRESMFIVNNFARDYFQNILKNHVDGRSIGLAYFRQRGFRDDIIEKFQLGYC
TESHDAMSQEALRKGYKKEFLVKTGICYETDDHRLRDRFWGRVIFPVHTLSGKVVAFGGRVLSTATKGVKVKYVNSPESE
IYHKSNELYGIYFAKQAIVRQDRCFLVEGYTDVISMHQSGIENVVASSGTALTPGQIRLIHRFTNNITVLYDGDVAGIKA
SIRGIDMLLEEGMNIKVCLLPDGDDPDSFARKHNSEEFQAFIREHEKDFIRFKTDLLMEDAGRDPIKRAELISNIVRSIS
VIPEAIIRDVYIKECSQHLRIEEKLLVAEVAKLREAQAEKANRPSYNHSSSTTGDTSGTAAASGTPAYSGAPTYSTPSSP
YANAGMPPEPEYDDDGNIVSFADPMSAATGGAAGFPPGNAPASTTDTTVPGDSYTSFIPQEGKEGQEFYKFERLILQAVV
RYGEKVMCNLTDEEGNEIPVTVIEYVVNDLKEDDLAFHNPLHRQILTEAATHIHDAGFIAERYFLAHPDQTISRLSVDLI
NVRYQLSKYHSKSQKIVTDEERLYELVPMLMINFKYAIVTEELKHMLYALQDPALAHDNEKCNSLMQRYNEMREVQSLMA
KRLGDRVVLR

Sequences:

>Translated_730_residues
MIDQVTIDRILDAAQIVDVVSEFVTLRKRGVNYVGLCPFHNEKTPSFSVSPAKGLCKCFSCGKGGNSVHFIMEHEQMSYY
EALKYLAKKYNIEIKERELTNEEKQAQTTRESMFIVNNFARDYFQNILKNHVDGRSIGLAYFRQRGFRDDIIEKFQLGYC
TESHDAMSQEALRKGYKKEFLVKTGICYETDDHRLRDRFWGRVIFPVHTLSGKVVAFGGRVLSTATKGVKVKYVNSPESE
IYHKSNELYGIYFAKQAIVRQDRCFLVEGYTDVISMHQSGIENVVASSGTALTPGQIRLIHRFTNNITVLYDGDVAGIKA
SIRGIDMLLEEGMNIKVCLLPDGDDPDSFARKHNSEEFQAFIREHEKDFIRFKTDLLMEDAGRDPIKRAELISNIVRSIS
VIPEAIIRDVYIKECSQHLRIEEKLLVAEVAKLREAQAEKANRPSYNHSSSTTGDTSGTAAASGTPAYSGAPTYSTPSSP
YANAGMPPEPEYDDDGNIVSFADPMSAATGGAAGFPPGNAPASTTDTTVPGDSYTSFIPQEGKEGQEFYKFERLILQAVV
RYGEKVMCNLTDEEGNEIPVTVIEYVVNDLKEDDLAFHNPLHRQILTEAATHIHDAGFIAERYFLAHPDQTISRLSVDLI
NVRYQLSKYHSKSQKIVTDEERLYELVPMLMINFKYAIVTEELKHMLYALQDPALAHDNEKCNSLMQRYNEMREVQSLMA
KRLGDRVVLR
>Mature_730_residues
MIDQVTIDRILDAAQIVDVVSEFVTLRKRGVNYVGLCPFHNEKTPSFSVSPAKGLCKCFSCGKGGNSVHFIMEHEQMSYY
EALKYLAKKYNIEIKERELTNEEKQAQTTRESMFIVNNFARDYFQNILKNHVDGRSIGLAYFRQRGFRDDIIEKFQLGYC
TESHDAMSQEALRKGYKKEFLVKTGICYETDDHRLRDRFWGRVIFPVHTLSGKVVAFGGRVLSTATKGVKVKYVNSPESE
IYHKSNELYGIYFAKQAIVRQDRCFLVEGYTDVISMHQSGIENVVASSGTALTPGQIRLIHRFTNNITVLYDGDVAGIKA
SIRGIDMLLEEGMNIKVCLLPDGDDPDSFARKHNSEEFQAFIREHEKDFIRFKTDLLMEDAGRDPIKRAELISNIVRSIS
VIPEAIIRDVYIKECSQHLRIEEKLLVAEVAKLREAQAEKANRPSYNHSSSTTGDTSGTAAASGTPAYSGAPTYSTPSSP
YANAGMPPEPEYDDDGNIVSFADPMSAATGGAAGFPPGNAPASTTDTTVPGDSYTSFIPQEGKEGQEFYKFERLILQAVV
RYGEKVMCNLTDEEGNEIPVTVIEYVVNDLKEDDLAFHNPLHRQILTEAATHIHDAGFIAERYFLAHPDQTISRLSVDLI
NVRYQLSKYHSKSQKIVTDEERLYELVPMLMINFKYAIVTEELKHMLYALQDPALAHDNEKCNSLMQRYNEMREVQSLMA
KRLGDRVVLR

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=417, Percent_Identity=33.8129496402878, Blast_Score=254, Evalue=1e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 82404; Mature: 82404

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDQVTIDRILDAAQIVDVVSEFVTLRKRGVNYVGLCPFHNEKTPSFSVSPAKGLCKCFS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHH
CGKGGNSVHFIMEHEQMSYYEALKYLAKKYNIEIKERELTNEEKQAQTTRESMFIVNNFA
CCCCCCEEEEEEECHHHHHHHHHHHHHHHHCCEEEHHHHCCHHHHHHHHHHHHHHHHHHH
RDYFQNILKNHVDGRSIGLAYFRQRGFRDDIIEKFQLGYCTESHDAMSQEALRKGYKKEF
HHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHH
LVKTGICYETDDHRLRDRFWGRVIFPVHTLSGKVVAFGGRVLSTATKGVKVKYVNSPESE
HHHCCCEEECCCHHHHHHHCCEEEEEEEECCCCEEEECCCHHHHCCCCEEEEEECCCHHH
IYHKSNELYGIYFAKQAIVRQDRCFLVEGYTDVISMHQSGIENVVASSGTALTPGQIRLI
HHHCCCCEEEEEEHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEE
HRFTNNITVLYDGDVAGIKASIRGIDMLLEEGMNIKVCLLPDGDDPDSFARKHNSEEFQA
EEECCCEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHCCHHHHHH
FIREHEKDFIRFKTDLLMEDAGRDPIKRAELISNIVRSISVIPEAIIRDVYIKECSQHLR
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEEKLLVAEVAKLREAQAEKANRPSYNHSSSTTGDTSGTAAASGTPAYSGAPTYSTPSSP
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
YANAGMPPEPEYDDDGNIVSFADPMSAATGGAAGFPPGNAPASTTDTTVPGDSYTSFIPQ
CCCCCCCCCCCCCCCCCEEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCC
EGKEGQEFYKFERLILQAVVRYGEKVMCNLTDEEGNEIPVTVIEYVVNDLKEDDLAFHNP
CCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHCCH
LHRQILTEAATHIHDAGFIAERYFLAHPDQTISRLSVDLINVRYQLSKYHSKSQKIVTDE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
ERLYELVPMLMINFKYAIVTEELKHMLYALQDPALAHDNEKCNSLMQRYNEMREVQSLMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
KRLGDRVVLR
HHCCCCCCCC
>Mature Secondary Structure
MIDQVTIDRILDAAQIVDVVSEFVTLRKRGVNYVGLCPFHNEKTPSFSVSPAKGLCKCFS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHH
CGKGGNSVHFIMEHEQMSYYEALKYLAKKYNIEIKERELTNEEKQAQTTRESMFIVNNFA
CCCCCCEEEEEEECHHHHHHHHHHHHHHHHCCEEEHHHHCCHHHHHHHHHHHHHHHHHHH
RDYFQNILKNHVDGRSIGLAYFRQRGFRDDIIEKFQLGYCTESHDAMSQEALRKGYKKEF
HHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHH
LVKTGICYETDDHRLRDRFWGRVIFPVHTLSGKVVAFGGRVLSTATKGVKVKYVNSPESE
HHHCCCEEECCCHHHHHHHCCEEEEEEEECCCCEEEECCCHHHHCCCCEEEEEECCCHHH
IYHKSNELYGIYFAKQAIVRQDRCFLVEGYTDVISMHQSGIENVVASSGTALTPGQIRLI
HHHCCCCEEEEEEHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEE
HRFTNNITVLYDGDVAGIKASIRGIDMLLEEGMNIKVCLLPDGDDPDSFARKHNSEEFQA
EEECCCEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHCCHHHHHH
FIREHEKDFIRFKTDLLMEDAGRDPIKRAELISNIVRSISVIPEAIIRDVYIKECSQHLR
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEEKLLVAEVAKLREAQAEKANRPSYNHSSSTTGDTSGTAAASGTPAYSGAPTYSTPSSP
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
YANAGMPPEPEYDDDGNIVSFADPMSAATGGAAGFPPGNAPASTTDTTVPGDSYTSFIPQ
CCCCCCCCCCCCCCCCCEEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCC
EGKEGQEFYKFERLILQAVVRYGEKVMCNLTDEEGNEIPVTVIEYVVNDLKEDDLAFHNP
CCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHCCH
LHRQILTEAATHIHDAGFIAERYFLAHPDQTISRLSVDLINVRYQLSKYHSKSQKIVTDE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
ERLYELVPMLMINFKYAIVTEELKHMLYALQDPALAHDNEKCNSLMQRYNEMREVQSLMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
KRLGDRVVLR
HHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7961408; 11466286 [H]