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The map label for this gene is ppnK

Identifier: 29349325

GI number: 29349325

Start: 5091961

End: 5092869

Strand: Reverse

Name: ppnK

Synonym: BT_3917

Alternate gene names: 29349325

Gene position: 5092869-5091961 (Counterclockwise)

Preceding gene: 29349340

Following gene: 29349319

Centisome position: 81.35

GC content: 50.72

Gene sequence:

>909_bases
ATGCCAATTTTGCAACCGAAACTAATGAAACAGCTTATGAAATTTGCCATTTTCGGAAATACGTATCAGCCTAAAAAGTC
TTTGCATGCACTCAGACTGTTCGAGCTTCTCAAGAAGCAGGGAGCGGAAATCTGTATGTGCCGGGAGTTTTACCAGTTCC
TGACGGCAGATTTAAAAATGGAAGTGCCTGTCGACGCCCTGCTCGAAGGCAACGACTTCACAGCCGATATGGTTATCAGC
ATCGGAGGTGACGGCACATTTCTGAAAGCTGCCCGCCGGGTGGGTAGAAAACAAATCCCCATTCTAGGCATCAACACAGG
GCGGCTGGGATTTCTTGCCGATGTTTCACCCGAAGAGATGGAGGTGACTTTCGAAGAGATTCAGGCTGGCAGATACAGTG
TGGAGGAACGAAGTGTATTGCAGCTTATCTGCAATGACAGAAATCTCCAGGAGTCGCCTTATGCCCTCAACGAAATTGCG
GTGCTCAAGCGGGACAGTTCTTCCATGATCAGCATCCGCACTGCGATCAACGGTGCTTATCTGAATACTTATCAGGCAGA
CGGACTGGTGATTGCCACTCCTACCGGTTCTACTGCGTATTCATTAAGCGTAGGCGGCCCGATCATCGTTCCTCATTCCA
ATACGATCGCCATTACCCCGGTAGCTCCGCACAGCCTCAACGTCCGTCCTATCGTCATTCGTGACGACTGGGAAATCACG
CTGGACGTGGAAAGCCGCAGCCATAACTTCCTCGTTGCCATCGACGGCAGCAGCGAAACGTGCAAGGAGACAACTCAGCT
GACCATTCGCCGGGCAGACTACAGTATAAAAGTAGTGAAACGGTTCAATCATATTTTCTTCGATACCCTCCGCAGCAAGA
TGATGTGGGGAGCGGACGGAAGGCGCTGA

Upstream 100 bases:

>100_bases
TATTTGCCGCAAAAATACAAATATTATTATTAGTTGCCTAATAATACGAAACGCATTCTCAGTTCTTTTTTGGTTATTTA
AAGTAGAAATCCAAGAAAAA

Downstream 100 bases:

>100_bases
TCTTCCGACCGGACCTTCGTAACTATCTGATTCCGGACCTTCGGGCATAAAAAAGTACATCCTTCCTCACCAGGACAGAA
TGTACTTAAACAAAAACAAA

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MPILQPKLMKQLMKFAIFGNTYQPKKSLHALRLFELLKKQGAEICMCREFYQFLTADLKMEVPVDALLEGNDFTADMVIS
IGGDGTFLKAARRVGRKQIPILGINTGRLGFLADVSPEEMEVTFEEIQAGRYSVEERSVLQLICNDRNLQESPYALNEIA
VLKRDSSSMISIRTAINGAYLNTYQADGLVIATPTGSTAYSLSVGGPIIVPHSNTIAITPVAPHSLNVRPIVIRDDWEIT
LDVESRSHNFLVAIDGSSETCKETTQLTIRRADYSIKVVKRFNHIFFDTLRSKMMWGADGRR

Sequences:

>Translated_302_residues
MPILQPKLMKQLMKFAIFGNTYQPKKSLHALRLFELLKKQGAEICMCREFYQFLTADLKMEVPVDALLEGNDFTADMVIS
IGGDGTFLKAARRVGRKQIPILGINTGRLGFLADVSPEEMEVTFEEIQAGRYSVEERSVLQLICNDRNLQESPYALNEIA
VLKRDSSSMISIRTAINGAYLNTYQADGLVIATPTGSTAYSLSVGGPIIVPHSNTIAITPVAPHSLNVRPIVIRDDWEIT
LDVESRSHNFLVAIDGSSETCKETTQLTIRRADYSIKVVKRFNHIFFDTLRSKMMWGADGRR
>Mature_301_residues
PILQPKLMKQLMKFAIFGNTYQPKKSLHALRLFELLKKQGAEICMCREFYQFLTADLKMEVPVDALLEGNDFTADMVISI
GGDGTFLKAARRVGRKQIPILGINTGRLGFLADVSPEEMEVTFEEIQAGRYSVEERSVLQLICNDRNLQESPYALNEIAV
LKRDSSSMISIRTAINGAYLNTYQADGLVIATPTGSTAYSLSVGGPIIVPHSNTIAITPVAPHSLNVRPIVIRDDWEITL
DVESRSHNFLVAIDGSSETCKETTQLTIRRADYSIKVVKRFNHIFFDTLRSKMMWGADGRR

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Homo sapiens, GI55743112, Length=253, Percent_Identity=28.0632411067194, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1788968, Length=224, Percent_Identity=34.8214285714286, Blast_Score=155, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6320794, Length=250, Percent_Identity=30, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6325068, Length=175, Percent_Identity=34.8571428571429, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6322509, Length=282, Percent_Identity=27.6595744680851, Blast_Score=105, Evalue=6e-24,
Organism=Drosophila melanogaster, GI28573832, Length=250, Percent_Identity=27.2, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI28573826, Length=250, Percent_Identity=27.2, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI28573828, Length=250, Percent_Identity=27.2, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI28573830, Length=250, Percent_Identity=27.2, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI161077047, Length=250, Percent_Identity=27.2, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI281363323, Length=275, Percent_Identity=25.0909090909091, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24653424, Length=275, Percent_Identity=25.0909090909091, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281363321, Length=275, Percent_Identity=25.0909090909091, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI20129957, Length=275, Percent_Identity=25.0909090909091, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24653422, Length=275, Percent_Identity=25.0909090909091, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK_BACTN (Q8A0V4)

Other databases:

- EMBL:   AE015928
- RefSeq:   NP_812828.1
- GeneID:   1075328
- GenomeReviews:   AE015928_GR
- KEGG:   bth:BT_3917
- NMPDR:   fig|226186.1.peg.3915
- HOGENOM:   HBG713904
- OMA:   YLMASSS
- PhylomeDB:   Q8A0V4
- ProtClustDB:   PRK01911
- BioCyc:   BTHE226186:BT_3917-MONOMER
- BRENDA:   2.7.1.23
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 33778; Mature: 33646

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPILQPKLMKQLMKFAIFGNTYQPKKSLHALRLFELLKKQGAEICMCREFYQFLTADLKM
CCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCEE
EVPVDALLEGNDFTADMVISIGGDGTFLKAARRVGRKQIPILGINTGRLGFLADVSPEEM
ECCHHHEECCCCCCEEEEEEECCCCHHHHHHHHCCCCCCCEEEECCCCEEEEECCCCHHH
EVTFEEIQAGRYSVEERSVLQLICNDRNLQESPYALNEIAVLKRDSSSMISIRTAINGAY
EEEHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCEE
LNTYQADGLVIATPTGSTAYSLSVGGPIIVPHSNTIAITPVAPHSLNVRPIVIRDDWEIT
EEEEECCCEEEECCCCCCEEEEECCCEEEEECCCEEEEEECCCCCCCEEEEEEECCCEEE
LDVESRSHNFLVAIDGSSETCKETTQLTIRRADYSIKVVKRFNHIFFDTLRSKMMWGADG
EEEECCCCEEEEEECCCCHHHHHHHEEEEEECCCEEEHHHHHHHHHHHHHHHHHCCCCCC
RR
CC
>Mature Secondary Structure 
PILQPKLMKQLMKFAIFGNTYQPKKSLHALRLFELLKKQGAEICMCREFYQFLTADLKM
CCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCEE
EVPVDALLEGNDFTADMVISIGGDGTFLKAARRVGRKQIPILGINTGRLGFLADVSPEEM
ECCHHHEECCCCCCEEEEEEECCCCHHHHHHHHCCCCCCCEEEECCCCEEEEECCCCHHH
EVTFEEIQAGRYSVEERSVLQLICNDRNLQESPYALNEIAVLKRDSSSMISIRTAINGAY
EEEHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCEE
LNTYQADGLVIATPTGSTAYSLSVGGPIIVPHSNTIAITPVAPHSLNVRPIVIRDDWEIT
EEEEECCCEEEECCCCCCEEEEECCCEEEEECCCEEEEEECCCCCCCEEEEEEECCCEEE
LDVESRSHNFLVAIDGSSETCKETTQLTIRRADYSIKVVKRFNHIFFDTLRSKMMWGADG
EEEECCCCEEEEEECCCCHHHHHHHEEEEEECCCEEEHHHHHHHHHHHHHHHHHCCCCCC
RR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928