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The map label for this gene is mdh [H]

Identifier: 29349319

GI number: 29349319

Start: 5085583

End: 5086524

Strand: Reverse

Name: mdh [H]

Synonym: BT_3911

Alternate gene names: 29349319

Gene position: 5086524-5085583 (Counterclockwise)

Preceding gene: 29349325

Following gene: 29349313

Centisome position: 81.25

GC content: 45.22

Gene sequence:

>942_bases
ATGTCAAAAGTAACCGTAGTAGGCGCAGGTAACGTAGGTGCTACATGTGCAAATGTACTTGCTTTCAATGAAGTAGCAGA
CGAAGTAGTAATGCTGGACGTTAAAGAAGGCGTTTCAGAAGGTAAAGCAATGGATATGATGCAGACAGCTCAACTGTTGG
GTTTCGACACAACTGTAGTAGGCTGCACTAACGACTATGCTCAGACTGCTAACTCTGACGTTGTTGTAATTACTTCAGGT
ATTCCTCGTAAACCGGGTATGACTCGCGAAGAACTGATCGGTGTAAACGCTGGTATCGTTAAATCAGTGGCAGAAAACAT
CTTGAAATATTCTCCCAATGCAATCCTTGTTGTTATCTCTAACCCGATGGATACAATGACTTACCTGTCATTGAAAGCTC
TGGGCTTGCCGAAGAACCGCATCATCGGTATGGGTGGCGCACTGGACAGCTCTCGTTTCAAATATTTCTTGTCTCAGGCT
TTGGGCTGCAACGCCAACGAAGTAGAAGGTATGGTTATCGGTGGTCACGGTGATACTACTATGATTCCTTTGACTCGCTT
CGCTACTTACAAAGGTATGCCTGTAACTAACTTCATCTCTGAAGAAAAACTGAACGAAGTGGCTGCTGCTACTATGGTAG
GTGGTGCTACTCTGACTAAGTTACTGGGCACTTCTGCATGGTATGCACCGGGTGCAGCAGGAGCATTCGTAGTTGAATCT
ATCCTTCACGACCAAAAGAAGATGATCCCTTGTTCAGTATATCTGGAAGGAGAATACGGTGAATCAGACATTTGCATCGG
TGTTCCTGTAATCCTTGGCAAGAACGGTATCGAAAAGATCGTCGAACTGGATCTGAACGCTGACGAAAAAGCTAAGTTCG
CTGCCAGCGCAAAAGCTGTTCACGGAACAAACGCTGCTTTGAAAGAAGTAGGTGCTCTGTAA

Upstream 100 bases:

>100_bases
AAATTATTACGGAAAAATTTCTTTAATTAGGATTATCGTTGTAATTTTGTCGCGCTAAAAAGCATGAAATAAATTATCAA
CTTTTTAAATATATAGTATT

Downstream 100 bases:

>100_bases
TTAGCAATCCCCTTACATTTATATAAGCTAAGAAAGGCTGTCCTCCGCTATGAGGGACAGCCTTCTTTCTTTTCTATCCA
ATAGCTGTCATTCACTCTTT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MSKVTVVGAGNVGATCANVLAFNEVADEVVMLDVKEGVSEGKAMDMMQTAQLLGFDTTVVGCTNDYAQTANSDVVVITSG
IPRKPGMTREELIGVNAGIVKSVAENILKYSPNAILVVISNPMDTMTYLSLKALGLPKNRIIGMGGALDSSRFKYFLSQA
LGCNANEVEGMVIGGHGDTTMIPLTRFATYKGMPVTNFISEEKLNEVAAATMVGGATLTKLLGTSAWYAPGAAGAFVVES
ILHDQKKMIPCSVYLEGEYGESDICIGVPVILGKNGIEKIVELDLNADEKAKFAASAKAVHGTNAALKEVGAL

Sequences:

>Translated_313_residues
MSKVTVVGAGNVGATCANVLAFNEVADEVVMLDVKEGVSEGKAMDMMQTAQLLGFDTTVVGCTNDYAQTANSDVVVITSG
IPRKPGMTREELIGVNAGIVKSVAENILKYSPNAILVVISNPMDTMTYLSLKALGLPKNRIIGMGGALDSSRFKYFLSQA
LGCNANEVEGMVIGGHGDTTMIPLTRFATYKGMPVTNFISEEKLNEVAAATMVGGATLTKLLGTSAWYAPGAAGAFVVES
ILHDQKKMIPCSVYLEGEYGESDICIGVPVILGKNGIEKIVELDLNADEKAKFAASAKAVHGTNAALKEVGAL
>Mature_312_residues
SKVTVVGAGNVGATCANVLAFNEVADEVVMLDVKEGVSEGKAMDMMQTAQLLGFDTTVVGCTNDYAQTANSDVVVITSGI
PRKPGMTREELIGVNAGIVKSVAENILKYSPNAILVVISNPMDTMTYLSLKALGLPKNRIIGMGGALDSSRFKYFLSQAL
GCNANEVEGMVIGGHGDTTMIPLTRFATYKGMPVTNFISEEKLNEVAAATMVGGATLTKLLGTSAWYAPGAAGAFVVESI
LHDQKKMIPCSVYLEGEYGESDICIGVPVILGKNGIEKIVELDLNADEKAKFAASAKAVHGTNAALKEVGAL

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family [H]

Homologues:

Organism=Homo sapiens, GI47059044, Length=315, Percent_Identity=34.2857142857143, Blast_Score=199, Evalue=4e-51,
Organism=Homo sapiens, GI221136809, Length=315, Percent_Identity=34.2857142857143, Blast_Score=199, Evalue=4e-51,
Organism=Homo sapiens, GI5031857, Length=315, Percent_Identity=35.2380952380952, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI260099723, Length=315, Percent_Identity=35.2380952380952, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI15082234, Length=315, Percent_Identity=32.6984126984127, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI9257228, Length=313, Percent_Identity=32.5878594249201, Blast_Score=185, Evalue=6e-47,
Organism=Homo sapiens, GI4504973, Length=313, Percent_Identity=32.5878594249201, Blast_Score=185, Evalue=6e-47,
Organism=Homo sapiens, GI291575128, Length=323, Percent_Identity=33.1269349845201, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI4557032, Length=323, Percent_Identity=33.1269349845201, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI260099727, Length=180, Percent_Identity=41.1111111111111, Blast_Score=148, Evalue=6e-36,
Organism=Homo sapiens, GI260099725, Length=180, Percent_Identity=41.1111111111111, Blast_Score=148, Evalue=6e-36,
Organism=Homo sapiens, GI103472011, Length=311, Percent_Identity=28.2958199356913, Blast_Score=124, Evalue=8e-29,
Organism=Homo sapiens, GI207028494, Length=199, Percent_Identity=32.1608040201005, Blast_Score=114, Evalue=8e-26,
Organism=Homo sapiens, GI21735621, Length=218, Percent_Identity=33.9449541284404, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI103472015, Length=182, Percent_Identity=30.2197802197802, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1789632, Length=290, Percent_Identity=31.3793103448276, Blast_Score=99, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17535107, Length=299, Percent_Identity=30.1003344481605, Blast_Score=140, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17554310, Length=299, Percent_Identity=32.7759197324415, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6322765, Length=306, Percent_Identity=30.3921568627451, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320125, Length=345, Percent_Identity=28.4057971014493, Blast_Score=87, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324446, Length=170, Percent_Identity=32.9411764705882, Blast_Score=66, Evalue=9e-12,
Organism=Drosophila melanogaster, GI17136226, Length=314, Percent_Identity=32.484076433121, Blast_Score=172, Evalue=2e-43,
Organism=Drosophila melanogaster, GI45550422, Length=307, Percent_Identity=28.6644951140065, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24647881, Length=311, Percent_Identity=33.7620578778135, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24663599, Length=321, Percent_Identity=28.0373831775701, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24663595, Length=279, Percent_Identity=29.0322580645161, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 32763; Mature: 32632

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00356 HTH_LACI_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVTVVGAGNVGATCANVLAFNEVADEVVMLDVKEGVSEGKAMDMMQTAQLLGFDTTVV
CCEEEEEECCCCCHHHHHHHHHHHHHHHEEEEEHHHCCCCCHHHHHHHHHHHHCCCEEEE
GCTNDYAQTANSDVVVITSGIPRKPGMTREELIGVNAGIVKSVAENILKYSPNAILVVIS
ECCCHHHHCCCCCEEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEC
NPMDTMTYLSLKALGLPKNRIIGMGGALDSSRFKYFLSQALGCNANEVEGMVIGGHGDTT
CCCCHHHHHHHHCCCCCCHHEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCE
MIPLTRFATYKGMPVTNFISEEKLNEVAAATMVGGATLTKLLGTSAWYAPGAAGAFVVES
EEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCHHHHHHH
ILHDQKKMIPCSVYLEGEYGESDICIGVPVILGKNGIEKIVELDLNADEKAKFAASAKAV
HHHHHHHCCCEEEEEECCCCCCCEEEECCCEECCCHHHHHHEECCCCCCHHHHHHHHHHH
HGTNAALKEVGAL
CCCCHHHHHHCCC
>Mature Secondary Structure 
SKVTVVGAGNVGATCANVLAFNEVADEVVMLDVKEGVSEGKAMDMMQTAQLLGFDTTVV
CEEEEEECCCCCHHHHHHHHHHHHHHHEEEEEHHHCCCCCHHHHHHHHHHHHCCCEEEE
GCTNDYAQTANSDVVVITSGIPRKPGMTREELIGVNAGIVKSVAENILKYSPNAILVVIS
ECCCHHHHCCCCCEEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEC
NPMDTMTYLSLKALGLPKNRIIGMGGALDSSRFKYFLSQALGCNANEVEGMVIGGHGDTT
CCCCHHHHHHHHCCCCCCHHEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCE
MIPLTRFATYKGMPVTNFISEEKLNEVAAATMVGGATLTKLLGTSAWYAPGAAGAFVVES
EEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCHHHHHHH
ILHDQKKMIPCSVYLEGEYGESDICIGVPVILGKNGIEKIVELDLNADEKAKFAASAKAV
HHHHHHHCCCEEEEEECCCCCCCEEEECCCEECCCHHHHHHEECCCCCCHHHHHHHHHHH
HGTNAALKEVGAL
CCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA