Click here to switch to the map view.

The map label for this gene is aprF [H]

Identifier: 29349313

GI number: 29349313

Start: 5076523

End: 5077986

Strand: Reverse

Name: aprF [H]

Synonym: BT_3905

Alternate gene names: 29349313

Gene position: 5077986-5076523 (Counterclockwise)

Preceding gene: 29349319

Following gene: 29349312

Centisome position: 81.11

GC content: 46.17

Gene sequence:

>1464_bases
ATGAAAAAACTATTTCTTCTGACAATTCTGTTGAGCCTGACTTTTATAGTAAAAGCGCAAAGCTTCCTAAGCCTAGATAG
TTGCCGCGCATTAGCTCTTGCCAACAACAAAGATCTGTTGATAAGCAATGAAAAAATAAATGCCGCTCATTATCAGCACA
AAGCTGCATTTACCAACTACCTGCCCAGTTTCTCGGCTACGGGAACTTACATGAGAAATCAAAAAGAATTTTCATTATTG
AACAATGACCAGAAAGCGGCGCTTTCGGGATTGGGGACCAATTTGGCCGGTCCGATACAACAGACGGCTACAGAGATTGC
TACCGCACACCCGGAGTTGAGACCGCTTATCGCTTCTTTAAGTGAAAAGTTAGGAGCTGCCTTACCCGCCCTCGACCAGG
CAGGAAACTCACTGGTGGATGCGCTCCGCACAGATACAAGAAATGTATATGCAGGTGCCATCACGCTGACACAGCCATTA
TACATGGGCGGCAAAATCCGTGCATATAATAAAATCACAAAGTATGCCGAAGAATTAGCGCAACAGCAACATCAAGGAGG
CATGCAGGAGGTTATCATGAGCACTGACCAGGCTTACTGGCAAGTCATATCCTTAGTGAACAAGAAGAAACTGGCCGAAG
GCTATCTCAAACTTTTGCAGCAGCTGGACAGCGATGTGGAAAAGATGATTGCCGAAGGAGTTGCCACAAAAGCCGACGGT
TTGTCCGTGCGTGTGAAAGTCAACGAAGCGGAAATGACACTGACTAAAGTGGAAGACGGATTAAGCCTGGCCCGAATGTT
GTTGTGTCAACTATGTGGCATTGATCTCAGCTCTCCTATCACGTTGGCAGACGAAAATATGGAGGACATTCCACTGCTCA
CACCGGAGACCCACTTCGACATGTCCACCGCTTACGCCAACCGTCCGGAGATACGCAGCCTTGAACTGGCTACTCAGATT
TATAAACAGAAAATAAATGTTACCCGCGCCGAACATCTTCCGTCCATTGCGTTGATGGGAAATTATATGGTGACCAATCC
TTCCGTATTCAACAGCTTTGAGAATAAATTCAAAGGAATGTGGAATGTGGGCGTTATGGTACAGATACCTATCTGGCATT
GGGGGGAAGGCATTTACAAGACCAAAGCCGCCAAGGCAGAAGCACGAATCGCACAATATCAGCTTCAGGATGCGAGAGAA
AAGATCGAATTGCAAGTTAACCAATCCGCTTTTAAAGTAAAGGAAGCCAGCAAAAAACTGGTAATGGCAACCAAGAATAT
GGAAAAGGCTGACGAAAATCTCCGTTATGCCACCTTAGGCTTCAAAGAAGGGGTCATTGCCACCAGCAATGTCCTGGAAG
CACAAACGGCATGGCTGTCCGCCCAATCGGAAAAGATTGACGCACAAATCGACGTGAAGCTGACGGAGATTTATCTGAAG
AAGTCTTTGGGAACGCTGAAATAA

Upstream 100 bases:

>100_bases
AGTCAACAAGTATAAAAAAATGAACTATCTCAATAAACAATTTATATGATAACGGGGTTATTTAAAAAAGAAGTATCACC
TTATTAATATGTAAGATGCG

Downstream 100 bases:

>100_bases
TGGACAATGGACAATTGACAATTAAAGACTGAGGTATTCAATCAATTTATTATTCGTAATTTAAAATTCATCAATAATAT
GGCACCTATAAAATCACAAA

Product: putative alkaline protease aprF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MKKLFLLTILLSLTFIVKAQSFLSLDSCRALALANNKDLLISNEKINAAHYQHKAAFTNYLPSFSATGTYMRNQKEFSLL
NNDQKAALSGLGTNLAGPIQQTATEIATAHPELRPLIASLSEKLGAALPALDQAGNSLVDALRTDTRNVYAGAITLTQPL
YMGGKIRAYNKITKYAEELAQQQHQGGMQEVIMSTDQAYWQVISLVNKKKLAEGYLKLLQQLDSDVEKMIAEGVATKADG
LSVRVKVNEAEMTLTKVEDGLSLARMLLCQLCGIDLSSPITLADENMEDIPLLTPETHFDMSTAYANRPEIRSLELATQI
YKQKINVTRAEHLPSIALMGNYMVTNPSVFNSFENKFKGMWNVGVMVQIPIWHWGEGIYKTKAAKAEARIAQYQLQDARE
KIELQVNQSAFKVKEASKKLVMATKNMEKADENLRYATLGFKEGVIATSNVLEAQTAWLSAQSEKIDAQIDVKLTEIYLK
KSLGTLK

Sequences:

>Translated_487_residues
MKKLFLLTILLSLTFIVKAQSFLSLDSCRALALANNKDLLISNEKINAAHYQHKAAFTNYLPSFSATGTYMRNQKEFSLL
NNDQKAALSGLGTNLAGPIQQTATEIATAHPELRPLIASLSEKLGAALPALDQAGNSLVDALRTDTRNVYAGAITLTQPL
YMGGKIRAYNKITKYAEELAQQQHQGGMQEVIMSTDQAYWQVISLVNKKKLAEGYLKLLQQLDSDVEKMIAEGVATKADG
LSVRVKVNEAEMTLTKVEDGLSLARMLLCQLCGIDLSSPITLADENMEDIPLLTPETHFDMSTAYANRPEIRSLELATQI
YKQKINVTRAEHLPSIALMGNYMVTNPSVFNSFENKFKGMWNVGVMVQIPIWHWGEGIYKTKAAKAEARIAQYQLQDARE
KIELQVNQSAFKVKEASKKLVMATKNMEKADENLRYATLGFKEGVIATSNVLEAQTAWLSAQSEKIDAQIDVKLTEIYLK
KSLGTLK
>Mature_487_residues
MKKLFLLTILLSLTFIVKAQSFLSLDSCRALALANNKDLLISNEKINAAHYQHKAAFTNYLPSFSATGTYMRNQKEFSLL
NNDQKAALSGLGTNLAGPIQQTATEIATAHPELRPLIASLSEKLGAALPALDQAGNSLVDALRTDTRNVYAGAITLTQPL
YMGGKIRAYNKITKYAEELAQQQHQGGMQEVIMSTDQAYWQVISLVNKKKLAEGYLKLLQQLDSDVEKMIAEGVATKADG
LSVRVKVNEAEMTLTKVEDGLSLARMLLCQLCGIDLSSPITLADENMEDIPLLTPETHFDMSTAYANRPEIRSLELATQI
YKQKINVTRAEHLPSIALMGNYMVTNPSVFNSFENKFKGMWNVGVMVQIPIWHWGEGIYKTKAAKAEARIAQYQLQDARE
KIELQVNQSAFKVKEASKKLVMATKNMEKADENLRYATLGFKEGVIATSNVLEAQTAWLSAQSEKIDAQIDVKLTEIYLK
KSLGTLK

Specific function: Involved in the secretion of alkaline protease [H]

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

None

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010130 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 53962; Mature: 53962

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLFLLTILLSLTFIVKAQSFLSLDSCRALALANNKDLLISNEKINAAHYQHKAAFTNY
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHH
LPSFSATGTYMRNQKEFSLLNNDQKAALSGLGTNLAGPIQQTATEIATAHPELRPLIASL
CCCCCCCCHHHCCCHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHH
SEKLGAALPALDQAGNSLVDALRTDTRNVYAGAITLTQPLYMGGKIRAYNKITKYAEELA
HHHHHHHCCHHHHCCHHHHHHHHHCCCCEEEEEEEECCHHHCCCEEHHHHHHHHHHHHHH
QQQHQGGMQEVIMSTDQAYWQVISLVNKKKLAEGYLKLLQQLDSDVEKMIAEGVATKADG
HHHHCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LSVRVKVNEAEMTLTKVEDGLSLARMLLCQLCGIDLSSPITLADENMEDIPLLTPETHFD
EEEEEEECHHHEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCC
MSTAYANRPEIRSLELATQIYKQKINVTRAEHLPSIALMGNYMVTNPSVFNSFENKFKGM
CHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCEEEECCEEECCCHHHHHHHHHHCCC
WNVGVMVQIPIWHWGEGIYKTKAAKAEARIAQYQLQDAREKIELQVNQSAFKVKEASKKL
CCCCEEEEEECEECCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECHHHHHHHHHHHHH
VMATKNMEKADENLRYATLGFKEGVIATSNVLEAQTAWLSAQSEKIDAQIDVKLTEIYLK
EEECCCHHHHHCCCEEEEECCCCCCEECCHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHH
KSLGTLK
HHCCCCC
>Mature Secondary Structure
MKKLFLLTILLSLTFIVKAQSFLSLDSCRALALANNKDLLISNEKINAAHYQHKAAFTNY
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHH
LPSFSATGTYMRNQKEFSLLNNDQKAALSGLGTNLAGPIQQTATEIATAHPELRPLIASL
CCCCCCCCHHHCCCHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHH
SEKLGAALPALDQAGNSLVDALRTDTRNVYAGAITLTQPLYMGGKIRAYNKITKYAEELA
HHHHHHHCCHHHHCCHHHHHHHHHCCCCEEEEEEEECCHHHCCCEEHHHHHHHHHHHHHH
QQQHQGGMQEVIMSTDQAYWQVISLVNKKKLAEGYLKLLQQLDSDVEKMIAEGVATKADG
HHHHCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LSVRVKVNEAEMTLTKVEDGLSLARMLLCQLCGIDLSSPITLADENMEDIPLLTPETHFD
EEEEEEECHHHEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCC
MSTAYANRPEIRSLELATQIYKQKINVTRAEHLPSIALMGNYMVTNPSVFNSFENKFKGM
CHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCEEEECCEEECCCHHHHHHHHHHCCC
WNVGVMVQIPIWHWGEGIYKTKAAKAEARIAQYQLQDAREKIELQVNQSAFKVKEASKKL
CCCCEEEEEECEECCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECHHHHHHHHHHHHH
VMATKNMEKADENLRYATLGFKEGVIATSNVLEAQTAWLSAQSEKIDAQIDVKLTEIYLK
EEECCCHHHHHCCCEEEEECCCCCCEECCHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHH
KSLGTLK
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1427098; 10984043 [H]