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The map label for this gene is proA

Identifier: 29349126

GI number: 29349126

Start: 4827826

End: 4829079

Strand: Reverse

Name: proA

Synonym: BT_3718

Alternate gene names: 29349126

Gene position: 4829079-4827826 (Counterclockwise)

Preceding gene: 29349127

Following gene: 29349125

Centisome position: 77.14

GC content: 48.96

Gene sequence:

>1254_bases
ATGACTACAAATTTAAACGGAACTTTCGCTGCTGTACAAGCTGCCAGCCGGGAATTGGCATTGCTGAGTGACGACACCAT
CAATCAGATACTGAATGCGGTCGCTGATGCTACCATTGCCGAAACATCCTTTATCCTGTCAGAAAATGAAAAGGACCTGG
CACGGATGGACAAGAGTAACCCGAAGTACGACCGGTTAAAACTGACGGAAGAGAGGCTGAAAGGCATCGCTGCCGACACA
CGTAATGTAGCCACTCTCCCCTCCCCGCTGGGAAGGATTTTAAAGGAAACCTCCCGCCCCAATGGCATGAAGCTGACCAA
AGTCAGTGTACCCTTCGGAGTAATAGGCATCATCTATGAAGCGCGTCCTAATGTCAGCTTCGACGTCTTTTCCCTTTGCC
TGAAAAGTGGAAATGCCTGCATCCTGAAAGGTGGAAGTGACGCAGACGATTCCAACCGTGCCATCATCAGCGTCATTCAC
AAGGTACTTGAAAAGTTTCATGTCAACCCACATATAGTAGAACTGCTCCCGGCAGACAGAGAGGCTACGGCAGCATTACT
GAATGCGACAGGCTATGTAGACCTGATTATTCCGAGAGGAAGCAGCAATCTGATAAATTTCGTGCGCGAGAATGCACGGA
TTCCTGTCATTGAAACAGGAGCAGGTATCTGCCATACTTATTTTGATGAGTTCGGAGATACCCGGAAAGGAGCGGATATT
ATCCATAATGCCAAGACACGCCGTGTCAGTGTCTGCAATGCGCTCGACTGTACCATCATTCATGAGAAACGTCTGGGGGA
TTTGCCTGCGCTCTGCGATCAACTGAAAGAAAGCAAGGTTACTATCTATGCAGATACACAGGCTTATCAGGCACTGGAAG
GATATTATCCGGCTGAGTTGCTGCAACCTGCCACTCCGGAAAGCTTCGGAACAGAGTTTCTGGATTACAAGATGGCTGTC
AAAACGGTGAAAAGCTTTGAAGACGCACTGGGGCATATACAGGAAAACAGTTCAAGGCACAGCGAATGTATCGTGACCGA
AAACAAAGAGCGTGCCACCTTGTTTACTAAGATAGTAGATGCGGCATGCGTATATACCAACGTATCTACCGCTTTCACCG
ACGGTGCACAATTCGGCCTGGGAGCCGAAATCGGTATCAGTACTCAAAAGCTGCATGCCCGCGGTCCGATGGGACTGGAG
GAAATCACTTCCTACAAATGGGTGATCGAAGGCGACGGACAGACCAGATGGTAA

Upstream 100 bases:

>100_bases
CACAAGCCAGGGAAGCAATGGGCAAACATGGCAAAAAGCCTGTAGTACATTACGACTATCTGTATATCGAATAAAAAATA
TAAAACGTAAAAAGGAGCAA

Downstream 100 bases:

>100_bases
AGTGAGGAAACGACAAATGATCAATTGGACAATAACGATTAATGATAGATAAAATGAAGAAATTTACTTGTGTACAGGAC
ATCGGCGATTTAAAATCAGC

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MTTNLNGTFAAVQAASRELALLSDDTINQILNAVADATIAETSFILSENEKDLARMDKSNPKYDRLKLTEERLKGIAADT
RNVATLPSPLGRILKETSRPNGMKLTKVSVPFGVIGIIYEARPNVSFDVFSLCLKSGNACILKGGSDADDSNRAIISVIH
KVLEKFHVNPHIVELLPADREATAALLNATGYVDLIIPRGSSNLINFVRENARIPVIETGAGICHTYFDEFGDTRKGADI
IHNAKTRRVSVCNALDCTIIHEKRLGDLPALCDQLKESKVTIYADTQAYQALEGYYPAELLQPATPESFGTEFLDYKMAV
KTVKSFEDALGHIQENSSRHSECIVTENKERATLFTKIVDAACVYTNVSTAFTDGAQFGLGAEIGISTQKLHARGPMGLE
EITSYKWVIEGDGQTRW

Sequences:

>Translated_417_residues
MTTNLNGTFAAVQAASRELALLSDDTINQILNAVADATIAETSFILSENEKDLARMDKSNPKYDRLKLTEERLKGIAADT
RNVATLPSPLGRILKETSRPNGMKLTKVSVPFGVIGIIYEARPNVSFDVFSLCLKSGNACILKGGSDADDSNRAIISVIH
KVLEKFHVNPHIVELLPADREATAALLNATGYVDLIIPRGSSNLINFVRENARIPVIETGAGICHTYFDEFGDTRKGADI
IHNAKTRRVSVCNALDCTIIHEKRLGDLPALCDQLKESKVTIYADTQAYQALEGYYPAELLQPATPESFGTEFLDYKMAV
KTVKSFEDALGHIQENSSRHSECIVTENKERATLFTKIVDAACVYTNVSTAFTDGAQFGLGAEIGISTQKLHARGPMGLE
EITSYKWVIEGDGQTRW
>Mature_416_residues
TTNLNGTFAAVQAASRELALLSDDTINQILNAVADATIAETSFILSENEKDLARMDKSNPKYDRLKLTEERLKGIAADTR
NVATLPSPLGRILKETSRPNGMKLTKVSVPFGVIGIIYEARPNVSFDVFSLCLKSGNACILKGGSDADDSNRAIISVIHK
VLEKFHVNPHIVELLPADREATAALLNATGYVDLIIPRGSSNLINFVRENARIPVIETGAGICHTYFDEFGDTRKGADII
HNAKTRRVSVCNALDCTIIHEKRLGDLPALCDQLKESKVTIYADTQAYQALEGYYPAELLQPATPESFGTEFLDYKMAVK
TVKSFEDALGHIQENSSRHSECIVTENKERATLFTKIVDAACVYTNVSTAFTDGAQFGLGAEIGISTQKLHARGPMGLEE
ITSYKWVIEGDGQTRW

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family

Homologues:

Organism=Homo sapiens, GI62912457, Length=405, Percent_Identity=34.5679012345679, Blast_Score=235, Evalue=5e-62,
Organism=Homo sapiens, GI21361368, Length=405, Percent_Identity=34.5679012345679, Blast_Score=235, Evalue=5e-62,
Organism=Escherichia coli, GI1786438, Length=412, Percent_Identity=43.2038834951456, Blast_Score=316, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI17569969, Length=410, Percent_Identity=35.8536585365854, Blast_Score=244, Evalue=4e-65,
Organism=Caenorhabditis elegans, GI17569967, Length=410, Percent_Identity=35.8536585365854, Blast_Score=244, Evalue=4e-65,
Organism=Saccharomyces cerevisiae, GI6324899, Length=414, Percent_Identity=35.5072463768116, Blast_Score=233, Evalue=4e-62,
Organism=Drosophila melanogaster, GI21357643, Length=409, Percent_Identity=35.4523227383863, Blast_Score=253, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROA_BACTN (Q8A1E8)

Other databases:

- EMBL:   AE015928
- RefSeq:   NP_812629.1
- ProteinModelPortal:   Q8A1E8
- SMR:   Q8A1E8
- GeneID:   1075939
- GenomeReviews:   AE015928_GR
- KEGG:   bth:BT_3718
- NMPDR:   fig|226186.1.peg.3716
- HOGENOM:   HBG318080
- OMA:   QYPAACN
- PhylomeDB:   Q8A1E8
- ProtClustDB:   PRK00197
- BioCyc:   BTHE226186:BT_3718-MONOMER
- BRENDA:   1.2.1.41
- GO:   GO:0005737
- HAMAP:   MF_00412
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134
- Gene3D:   G3DSA:3.40.309.10
- Gene3D:   G3DSA:3.40.605.10
- PANTHER:   PTHR11063:SF1
- PIRSF:   PIRSF000151
- TIGRFAMs:   TIGR00407

Pfam domain/function: SSF53720 Aldehyde_DH/Histidinol_DH

EC number: =1.2.1.41

Molecular weight: Translated: 45645; Mature: 45514

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNLNGTFAAVQAASRELALLSDDTINQILNAVADATIAETSFILSENEKDLARMDKSN
CCCCCCCHHHHHHHCCCCEEEECCHHHHHHHHHHHCCHHHHHHHEECCCCHHHHHHCCCC
PKYDRLKLTEERLKGIAADTRNVATLPSPLGRILKETSRPNGMKLTKVSVPFGVIGIIYE
CCHHHHEECHHHHCCHHCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCEEEEEEEE
ARPNVSFDVFSLCLKSGNACILKGGSDADDSNRAIISVIHKVLEKFHVNPHIVELLPADR
CCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCC
EATAALLNATGYVDLIIPRGSSNLINFVRENARIPVIETGAGICHTYFDEFGDTRKGADI
HHHHHHHCCCCEEEEEEECCCHHHHHHHHCCCCCCEEECCCCHHHHHHHHHCCCCCCHHH
IHNAKTRRVSVCNALDCTIIHEKRLGDLPALCDQLKESKVTIYADTQAYQALEGYYPAEL
HHCCCCCEEECCCCCCEEEEEHHHCCCHHHHHHHHHCCCEEEEECCHHHHHHHCCCCHHH
LQPATPESFGTEFLDYKMAVKTVKSFEDALGHIQENSSRHSECIVTENKERATLFTKIVD
CCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHH
AACVYTNVSTAFTDGAQFGLGAEIGISTQKLHARGPMGLEEITSYKWVIEGDGQTRW
HHHHHCCCHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHCCEEEEEECCCCCCC
>Mature Secondary Structure 
TTNLNGTFAAVQAASRELALLSDDTINQILNAVADATIAETSFILSENEKDLARMDKSN
CCCCCCHHHHHHHCCCCEEEECCHHHHHHHHHHHCCHHHHHHHEECCCCHHHHHHCCCC
PKYDRLKLTEERLKGIAADTRNVATLPSPLGRILKETSRPNGMKLTKVSVPFGVIGIIYE
CCHHHHEECHHHHCCHHCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCEEEEEEEE
ARPNVSFDVFSLCLKSGNACILKGGSDADDSNRAIISVIHKVLEKFHVNPHIVELLPADR
CCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCC
EATAALLNATGYVDLIIPRGSSNLINFVRENARIPVIETGAGICHTYFDEFGDTRKGADI
HHHHHHHCCCCEEEEEEECCCHHHHHHHHCCCCCCEEECCCCHHHHHHHHHCCCCCCHHH
IHNAKTRRVSVCNALDCTIIHEKRLGDLPALCDQLKESKVTIYADTQAYQALEGYYPAEL
HHCCCCCEEECCCCCCEEEEEHHHCCCHHHHHHHHHCCCEEEEECCHHHHHHHCCCCHHH
LQPATPESFGTEFLDYKMAVKTVKSFEDALGHIQENSSRHSECIVTENKERATLFTKIVD
CCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHH
AACVYTNVSTAFTDGAQFGLGAEIGISTQKLHARGPMGLEEITSYKWVIEGDGQTRW
HHHHHCCCHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663928