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The map label for this gene is proB

Identifier: 29349127

GI number: 29349127

Start: 4829106

End: 4830188

Strand: Reverse

Name: proB

Synonym: BT_3719

Alternate gene names: 29349127

Gene position: 4830188-4829106 (Counterclockwise)

Preceding gene: 29349129

Following gene: 29349126

Centisome position: 77.16

GC content: 48.66

Gene sequence:

>1083_bases
ATGAAACAAGAGTTTACACGAATAGCAGTAAAAGTAGGCAGTAACGTATTGGCACGCCGTGACGGTACATTGGACGTCAC
CCGTATGTCGGCACTCGTTGACCAGATAGCAGAATTAAATAAATCCGGAGTAGAGATCATTCTCATTTCTTCGGGAGCAG
TTGCTTCGGGACGCAGCGAAATACACCCACAGAAAAAGCTCGACAGTGTAGACCAGCGCCAGTTATTCTCCGCCGTAGGA
CAGGCTAAATTAATAAACCGCTACTACGAACTTTTCCGTGAACACGGCATTGCGGTAGGTCAGGTGCTGACTACCAAAGA
GAATTTCAGTACCCGCCGCCATTATCTGAATCAGAAGAACTGTATGACGGTGATGCTCGAAAACGGCGTCATCCCCATCG
TCAATGAGAATGATACGATTTCGGTCAGCGAGCTTATGTTCACGGACAATGACGAACTTTCCGGGCTGATCGCTTCCATG
ATGGATGCGCAGGCACTGATTATCCTGAGTAACATAGACGGAATCTACAACGGTTCACCCTCCGATCCTGCCTCCGCTGT
CATTCGGGAGATCGAGCACGGAAAGGATTTATCCAACTATATCCAGGCTACCAAATCGAGCTTCGGCCGCGGCGGTATGC
TGACGAAAACGAATATTGCCCGCAAGGTAGCCGACGAAGGGATTACGGTCATCATCGCCAACGGAAAACGGGATAATATT
CTCGTAGACTTGTTGCAACAGCCGGACGATACAGTTTGCACGCGCTTTATCCCTTCCACCGAAGCGGTATCCAGTGTAAA
GAAATGGATTGCCCACAGCGAAGGATTCGCCAAAGGGGAAATTCATATTAACGAATGTGCCACGGATATCCTCAGTTCGG
AGAAGGCAGCCAGCATTCTTCCGGTCGGAATCACCCACATCGAAGGCGAATTTGAGAAAGACGATATTGTACGCATCATG
GACTTTCTGGGCAATCAGGTTGGTGTAGGAAAAGCGAACTGCGATTCCGCACAAGCCAGGGAAGCAATGGGCAAACATGG
CAAAAAGCCTGTAGTACATTACGACTATCTGTATATCGAATAA

Upstream 100 bases:

>100_bases
TTCTTTTATCTGTCAGGCTGCAAAGATAATTCTTTTCAGCCTGTTTTTGTAACATATCGACAAAAAGCTCTATATTTGTG
CTTTCAAATCGTTAGTAAGG

Downstream 100 bases:

>100_bases
AAAATATAAAACGTAAAAAGGAGCAAATGACTACAAATTTAAACGGAACTTTCGCTGCTGTACAAGCTGCCAGCCGGGAA
TTGGCATTGCTGAGTGACGA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKQEFTRIAVKVGSNVLARRDGTLDVTRMSALVDQIAELNKSGVEIILISSGAVASGRSEIHPQKKLDSVDQRQLFSAVG
QAKLINRYYELFREHGIAVGQVLTTKENFSTRRHYLNQKNCMTVMLENGVIPIVNENDTISVSELMFTDNDELSGLIASM
MDAQALIILSNIDGIYNGSPSDPASAVIREIEHGKDLSNYIQATKSSFGRGGMLTKTNIARKVADEGITVIIANGKRDNI
LVDLLQQPDDTVCTRFIPSTEAVSSVKKWIAHSEGFAKGEIHINECATDILSSEKAASILPVGITHIEGEFEKDDIVRIM
DFLGNQVGVGKANCDSAQAREAMGKHGKKPVVHYDYLYIE

Sequences:

>Translated_360_residues
MKQEFTRIAVKVGSNVLARRDGTLDVTRMSALVDQIAELNKSGVEIILISSGAVASGRSEIHPQKKLDSVDQRQLFSAVG
QAKLINRYYELFREHGIAVGQVLTTKENFSTRRHYLNQKNCMTVMLENGVIPIVNENDTISVSELMFTDNDELSGLIASM
MDAQALIILSNIDGIYNGSPSDPASAVIREIEHGKDLSNYIQATKSSFGRGGMLTKTNIARKVADEGITVIIANGKRDNI
LVDLLQQPDDTVCTRFIPSTEAVSSVKKWIAHSEGFAKGEIHINECATDILSSEKAASILPVGITHIEGEFEKDDIVRIM
DFLGNQVGVGKANCDSAQAREAMGKHGKKPVVHYDYLYIE
>Mature_360_residues
MKQEFTRIAVKVGSNVLARRDGTLDVTRMSALVDQIAELNKSGVEIILISSGAVASGRSEIHPQKKLDSVDQRQLFSAVG
QAKLINRYYELFREHGIAVGQVLTTKENFSTRRHYLNQKNCMTVMLENGVIPIVNENDTISVSELMFTDNDELSGLIASM
MDAQALIILSNIDGIYNGSPSDPASAVIREIEHGKDLSNYIQATKSSFGRGGMLTKTNIARKVADEGITVIIANGKRDNI
LVDLLQQPDDTVCTRFIPSTEAVSSVKKWIAHSEGFAKGEIHINECATDILSSEKAASILPVGITHIEGEFEKDDIVRIM
DFLGNQVGVGKANCDSAQAREAMGKHGKKPVVHYDYLYIE

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=265, Percent_Identity=28.6792452830189, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI62912457, Length=263, Percent_Identity=28.5171102661597, Blast_Score=106, Evalue=3e-23,
Organism=Escherichia coli, GI1786437, Length=353, Percent_Identity=31.1614730878187, Blast_Score=187, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17569969, Length=262, Percent_Identity=31.2977099236641, Blast_Score=131, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17569967, Length=262, Percent_Identity=31.2977099236641, Blast_Score=131, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6321822, Length=358, Percent_Identity=28.7709497206704, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6320506, Length=391, Percent_Identity=27.3657289002558, Blast_Score=134, Evalue=2e-32,
Organism=Drosophila melanogaster, GI21357643, Length=296, Percent_Identity=30.4054054054054, Blast_Score=129, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_BACTN (Q8A1E7)

Other databases:

- EMBL:   AE015928
- RefSeq:   NP_812630.1
- ProteinModelPortal:   Q8A1E7
- SMR:   Q8A1E7
- GeneID:   1072190
- GenomeReviews:   AE015928_GR
- KEGG:   bth:BT_3719
- NMPDR:   fig|226186.1.peg.3717
- HOGENOM:   HBG507643
- OMA:   LINTIDP
- ProtClustDB:   PRK05429
- BioCyc:   BTHE226186:BT_3719-MONOMER
- BRENDA:   2.7.2.11
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 39454; Mature: 39454

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQEFTRIAVKVGSNVLARRDGTLDVTRMSALVDQIAELNKSGVEIILISSGAVASGRSE
CCCHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHH
IHPQKKLDSVDQRQLFSAVGQAKLINRYYELFREHGIAVGQVLTTKENFSTRRHYLNQKN
CCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHEEECCHHHHHHHHHCCCCC
CMTVMLENGVIPIVNENDTISVSELMFTDNDELSGLIASMMDAQALIILSNIDGIYNGSP
CEEEEEECCEEEEECCCCCEEHHHEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCC
SDPASAVIREIEHGKDLSNYIQATKSSFGRGGMLTKTNIARKVADEGITVIIANGKRDNI
CCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCH
LVDLLQQPDDTVCTRFIPSTEAVSSVKKWIAHSEGFAKGEIHINECATDILSSEKAASIL
HHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHCCCCCCCEE
PVGITHIEGEFEKDDIVRIMDFLGNQVGVGKANCDSAQAREAMGKHGKKPVVHYDYLYIE
EECEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEEEC
>Mature Secondary Structure
MKQEFTRIAVKVGSNVLARRDGTLDVTRMSALVDQIAELNKSGVEIILISSGAVASGRSE
CCCHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHH
IHPQKKLDSVDQRQLFSAVGQAKLINRYYELFREHGIAVGQVLTTKENFSTRRHYLNQKN
CCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHEEECCHHHHHHHHHCCCCC
CMTVMLENGVIPIVNENDTISVSELMFTDNDELSGLIASMMDAQALIILSNIDGIYNGSP
CEEEEEECCEEEEECCCCCEEHHHEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCC
SDPASAVIREIEHGKDLSNYIQATKSSFGRGGMLTKTNIARKVADEGITVIIANGKRDNI
CCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCH
LVDLLQQPDDTVCTRFIPSTEAVSSVKKWIAHSEGFAKGEIHINECATDILSSEKAASIL
HHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHCCCCCCCEE
PVGITHIEGEFEKDDIVRIMDFLGNQVGVGKANCDSAQAREAMGKHGKKPVVHYDYLYIE
EECEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928