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The map label for this gene is agaA [H]

Identifier: 29349000

GI number: 29349000

Start: 4648534

End: 4650516

Strand: Reverse

Name: agaA [H]

Synonym: BT_3592

Alternate gene names: 29349000

Gene position: 4650516-4648534 (Counterclockwise)

Preceding gene: 29349001

Following gene: 29348999

Centisome position: 74.29

GC content: 43.87

Gene sequence:

>1983_bases
ATGCAGTACATTAAGCAGATAATGTTATTGGTTATACTAACGATTAGTTGGAAATGCCAGGCAGAAGAAGTAAAAGAAAT
TTGGCTGGACGAGTTAGGCGAAAGTTCTTATTACATACAGGATTGGGGACTCCCCCGAATCAACAAAGCAGTCACTATGA
CGCCATTGACTGTGAAAGGCATCGTCTATGAACGCGGCATAGGAACTCATGCAATCAGCCGTATGCTCTTTGATATCGGA
AAAAAAGCCAAGACGCTTTCCGGATTAGCAGGTGCAGATGACAATACCCCATTTGCTTGTAATCTTCAGTTCAAGATATT
AGGAGATCGGAAAGAACTATGGAGAAGCGGAATTATGAGAAAAGGAGATCCTGCCAAACCTTTCAATATCGATTTATCAG
GAATAGACAAAGTTTTATTATTGGTAGAGGAATGCGGAGATGGAATGATGTACGACCGCGCTGATTGGCTGAATGTCAAA
TTCACAACTCTCGGTGACGTACAGCCCATTCCTGTATGGCCCAAGCCCATTGCCAAAGAAAAATACATATTGACTCCACA
AAGTCCGGACGCTCCACAAATCAATAACCCACTGACTTATGGCGCTCGTCCCGGCAATCCTTTTCTCATGCCAATTATGG
TTAGCGGCAAACGACCTATGACCTATAAAGCCAAAGGACTTCCGAAAGGATTAAAGTTAAACCGGAAAACAGGACTCATC
ACCGGAAGCACGAATACAAATGGTAACTTTAAGGTCCGCCTACAAGCGACAAATGAGAAAGGAACAGATGAAAAGGAGAT
CACACTGAAAATAGGTAGCGAGATTATGCTTACTCCTCCTATGGGCTGGAATAGCTGGAACTGCTGGCGATTTGCAGCTG
ATGACCAAAAGGTACGTGATGCTGCCCGAATCATGCACGAAAAGCTTCAGGCCTACGGATGGACTTATGTGAACATAGAT
GACGGATGGGAAGCCGATGAACGTACACCCGAAGGTGAACTCCCGGCTAACGAAAAATTTCCGGACTTTAAGACACTTAC
TGACTACATTCACTCGTTAGGGCTGAAATTCGGAATCTACTCTTCACCAGGTTGGACGACCTGCGGCAGGCATATAGGCA
GCTGTCAGCACGAATTGACAGATGCAAAAACTTGGGAAAAGTGGGGAGTAGACTATTTGAAATACGATTATTGCGGTTAT
GCTGCCATTGAGAAGAACTCGGAAGAGAAAACGATTCAAGAACCATTTATCGTCATGCGCAACGCTTTAGATCAGATAAA
ACGGGATATCGTATATTGTGTCGGATACGGAGCTCCCAATGTATGGAACTGGGGAGCGGAAGCCGGAGGTAATTTGTGGC
GAACAACCCGTGATATCAACGACCAATGGAATATTGTAATGGCAATCGGATGTTTCCAGGATGTTTGCGCATACGTATCA
GCACCTGGAAAATATAACGATCCGGATATGTTGGTAGTAGGCAAATTAGGACCGGGATGGGGTGCAAAATCCCACGATTC
GGACTTAACCGCTGACGAACAATATGCACATATCTCCCTATGGTCCATCCTTTCCGCCCCATTATTGCTTGGTTGTGACA
TGACTGCCATAGACGATTTCACGTTAGGTCTTCTGACCAATCCGGAAGTGATTGCAGTCAATCAGGACCCACTGGTAGCC
CCTGCTACTAAGCTCACAGTTCCCAACGGACAAATATGGTATAAAAAGTTATACGATGGTTCCTATGCACTCGGCTTCTT
CCAGATGGATCCCTACTTCATTTTATGGGATCAGGATAAAGCAGTAAATATCCAGCAACAAAAATATAACTTTAACTTTG
CCCTTAATCAGTTGGGCATCCAAGGTAAAGTCAAAATCAGAGACTTATGGAGACAAAAAAACTTAGGAATATTCAGTGGT
AGTTATGAAACTTCCATCCCCTACCACGGTGTCTCCCTAATTAAAATAACACCTATAAAATAA

Upstream 100 bases:

>100_bases
AGCAACAGTAGACGGTATTCATTTCACAGACTTAGGAATGATGAGATATGCAGACCTTGTTTGTCCGGTTATAAAGAAAG
TATTAAAAAAATAATTTACT

Downstream 100 bases:

>100_bases
AAGCAAATATGAAACGTATTCTATTTATTCTCTTTCTCTGTTGTATAGGTAGTGGTCTTGCTGCTTGCCAAGACGAAGAC
CACACGGTCCCTACCCTCCC

Product: alpha-galactosidase

Products: NA

Alternate protein names: Alpha-D-galactoside galactohydrolase; Alpha-galactosidase 27A; Melibiase [H]

Number of amino acids: Translated: 660; Mature: 660

Protein sequence:

>660_residues
MQYIKQIMLLVILTISWKCQAEEVKEIWLDELGESSYYIQDWGLPRINKAVTMTPLTVKGIVYERGIGTHAISRMLFDIG
KKAKTLSGLAGADDNTPFACNLQFKILGDRKELWRSGIMRKGDPAKPFNIDLSGIDKVLLLVEECGDGMMYDRADWLNVK
FTTLGDVQPIPVWPKPIAKEKYILTPQSPDAPQINNPLTYGARPGNPFLMPIMVSGKRPMTYKAKGLPKGLKLNRKTGLI
TGSTNTNGNFKVRLQATNEKGTDEKEITLKIGSEIMLTPPMGWNSWNCWRFAADDQKVRDAARIMHEKLQAYGWTYVNID
DGWEADERTPEGELPANEKFPDFKTLTDYIHSLGLKFGIYSSPGWTTCGRHIGSCQHELTDAKTWEKWGVDYLKYDYCGY
AAIEKNSEEKTIQEPFIVMRNALDQIKRDIVYCVGYGAPNVWNWGAEAGGNLWRTTRDINDQWNIVMAIGCFQDVCAYVS
APGKYNDPDMLVVGKLGPGWGAKSHDSDLTADEQYAHISLWSILSAPLLLGCDMTAIDDFTLGLLTNPEVIAVNQDPLVA
PATKLTVPNGQIWYKKLYDGSYALGFFQMDPYFILWDQDKAVNIQQQKYNFNFALNQLGIQGKVKIRDLWRQKNLGIFSG
SYETSIPYHGVSLIKITPIK

Sequences:

>Translated_660_residues
MQYIKQIMLLVILTISWKCQAEEVKEIWLDELGESSYYIQDWGLPRINKAVTMTPLTVKGIVYERGIGTHAISRMLFDIG
KKAKTLSGLAGADDNTPFACNLQFKILGDRKELWRSGIMRKGDPAKPFNIDLSGIDKVLLLVEECGDGMMYDRADWLNVK
FTTLGDVQPIPVWPKPIAKEKYILTPQSPDAPQINNPLTYGARPGNPFLMPIMVSGKRPMTYKAKGLPKGLKLNRKTGLI
TGSTNTNGNFKVRLQATNEKGTDEKEITLKIGSEIMLTPPMGWNSWNCWRFAADDQKVRDAARIMHEKLQAYGWTYVNID
DGWEADERTPEGELPANEKFPDFKTLTDYIHSLGLKFGIYSSPGWTTCGRHIGSCQHELTDAKTWEKWGVDYLKYDYCGY
AAIEKNSEEKTIQEPFIVMRNALDQIKRDIVYCVGYGAPNVWNWGAEAGGNLWRTTRDINDQWNIVMAIGCFQDVCAYVS
APGKYNDPDMLVVGKLGPGWGAKSHDSDLTADEQYAHISLWSILSAPLLLGCDMTAIDDFTLGLLTNPEVIAVNQDPLVA
PATKLTVPNGQIWYKKLYDGSYALGFFQMDPYFILWDQDKAVNIQQQKYNFNFALNQLGIQGKVKIRDLWRQKNLGIFSG
SYETSIPYHGVSLIKITPIK
>Mature_660_residues
MQYIKQIMLLVILTISWKCQAEEVKEIWLDELGESSYYIQDWGLPRINKAVTMTPLTVKGIVYERGIGTHAISRMLFDIG
KKAKTLSGLAGADDNTPFACNLQFKILGDRKELWRSGIMRKGDPAKPFNIDLSGIDKVLLLVEECGDGMMYDRADWLNVK
FTTLGDVQPIPVWPKPIAKEKYILTPQSPDAPQINNPLTYGARPGNPFLMPIMVSGKRPMTYKAKGLPKGLKLNRKTGLI
TGSTNTNGNFKVRLQATNEKGTDEKEITLKIGSEIMLTPPMGWNSWNCWRFAADDQKVRDAARIMHEKLQAYGWTYVNID
DGWEADERTPEGELPANEKFPDFKTLTDYIHSLGLKFGIYSSPGWTTCGRHIGSCQHELTDAKTWEKWGVDYLKYDYCGY
AAIEKNSEEKTIQEPFIVMRNALDQIKRDIVYCVGYGAPNVWNWGAEAGGNLWRTTRDINDQWNIVMAIGCFQDVCAYVS
APGKYNDPDMLVVGKLGPGWGAKSHDSDLTADEQYAHISLWSILSAPLLLGCDMTAIDDFTLGLLTNPEVIAVNQDPLVA
PATKLTVPNGQIWYKKLYDGSYALGFFQMDPYFILWDQDKAVNIQQQKYNFNFALNQLGIQGKVKIRDLWRQKNLGIFSG
SYETSIPYHGVSLIKITPIK

Specific function: Hydrolyzes galactomannan found in plant cell wall, by cleaving alpha-1,6-D-galactose side-chains from the mannan backbone. Appears to act in synergy with mannanase (ManA) to elicit hydrolysis of galactomannan. Has greater activity against galactomannans w

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 27 family [H]

Homologues:

Organism=Homo sapiens, GI4557781, Length=382, Percent_Identity=30.3664921465969, Blast_Score=152, Evalue=8e-37,
Organism=Homo sapiens, GI4504009, Length=335, Percent_Identity=31.9402985074627, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17562946, Length=340, Percent_Identity=32.9411764705882, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24583253, Length=342, Percent_Identity=34.2105263157895, Blast_Score=160, Evalue=3e-39,
Organism=Drosophila melanogaster, GI19922382, Length=429, Percent_Identity=27.7389277389277, Blast_Score=140, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24654142, Length=429, Percent_Identity=27.7389277389277, Blast_Score=140, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013780
- InterPro:   IPR002241
- InterPro:   IPR017853
- InterPro:   IPR000111 [H]

Pfam domain/function: PF02065 Melibiase [H]

EC number: =3.2.1.22 [H]

Molecular weight: Translated: 74390; Mature: 74390

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYIKQIMLLVILTISWKCQAEEVKEIWLDELGESSYYIQDWGLPRINKAVTMTPLTVKG
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEECCEEEEE
IVYERGIGTHAISRMLFDIGKKAKTLSGLAGADDNTPFACNLQFKILGDRKELWRSGIMR
EEEECCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCCEEEEEEEEEECCHHHHHHHCCCC
KGDPAKPFNIDLSGIDKVLLLVEECGDGMMYDRADWLNVKFTTLGDVQPIPVWPKPIAKE
CCCCCCCEEEEHHHHHHHHHHHHHCCCCCEECCCCEEEEEEEEECCCCCCCCCCCCCCCC
KYILTPQSPDAPQINNPLTYGARPGNPFLMPIMVSGKRPMTYKAKGLPKGLKLNRKTGLI
EEEECCCCCCCCCCCCCEEECCCCCCCEEEEEEECCCCCCEEECCCCCCCCEECCCCEEE
TGSTNTNGNFKVRLQATNEKGTDEKEITLKIGSEIMLTPPMGWNSWNCWRFAADDQKVRD
ECCCCCCCCEEEEEEECCCCCCCCEEEEEEECCEEEEECCCCCCCCCEEEECCCCHHHHH
AARIMHEKLQAYGWTYVNIDDGWEADERTPEGELPANEKFPDFKTLTDYIHSLGLKFGIY
HHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEE
SSPGWTTCGRHIGSCQHELTDAKTWEKWGVDYLKYDYCGYAAIEKNSEEKTIQEPFIVMR
CCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCEEEECCCCEEEEECCCCHHHHHHHHHHHH
NALDQIKRDIVYCVGYGAPNVWNWGAEAGGNLWRTTRDINDQWNIVMAIGCFQDVCAYVS
HHHHHHHHCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEHHHHHHHHHHC
APGKYNDPDMLVVGKLGPGWGAKSHDSDLTADEQYAHISLWSILSAPLLLGCDMTAIDDF
CCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHCHHHCCCCCCEECCE
TLGLLTNPEVIAVNQDPLVAPATKLTVPNGQIWYKKLYDGSYALGFFQMDPYFILWDQDK
EEEEECCCCEEEECCCCCCCCCEEEECCCCCEEEEEECCCCEEEEEEEECCEEEEECCCC
AVNIQQQKYNFNFALNQLGIQGKVKIRDLWRQKNLGIFSGSYETSIPYHGVSLIKITPIK
CCCEEEEEEEEEEEEECCCCCCEEEHHHHHHHCCCCEEECCCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure
MQYIKQIMLLVILTISWKCQAEEVKEIWLDELGESSYYIQDWGLPRINKAVTMTPLTVKG
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEECCEEEEE
IVYERGIGTHAISRMLFDIGKKAKTLSGLAGADDNTPFACNLQFKILGDRKELWRSGIMR
EEEECCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCCEEEEEEEEEECCHHHHHHHCCCC
KGDPAKPFNIDLSGIDKVLLLVEECGDGMMYDRADWLNVKFTTLGDVQPIPVWPKPIAKE
CCCCCCCEEEEHHHHHHHHHHHHHCCCCCEECCCCEEEEEEEEECCCCCCCCCCCCCCCC
KYILTPQSPDAPQINNPLTYGARPGNPFLMPIMVSGKRPMTYKAKGLPKGLKLNRKTGLI
EEEECCCCCCCCCCCCCEEECCCCCCCEEEEEEECCCCCCEEECCCCCCCCEECCCCEEE
TGSTNTNGNFKVRLQATNEKGTDEKEITLKIGSEIMLTPPMGWNSWNCWRFAADDQKVRD
ECCCCCCCCEEEEEEECCCCCCCCEEEEEEECCEEEEECCCCCCCCCEEEECCCCHHHHH
AARIMHEKLQAYGWTYVNIDDGWEADERTPEGELPANEKFPDFKTLTDYIHSLGLKFGIY
HHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEE
SSPGWTTCGRHIGSCQHELTDAKTWEKWGVDYLKYDYCGYAAIEKNSEEKTIQEPFIVMR
CCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCEEEECCCCEEEEECCCCHHHHHHHHHHHH
NALDQIKRDIVYCVGYGAPNVWNWGAEAGGNLWRTTRDINDQWNIVMAIGCFQDVCAYVS
HHHHHHHHCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEHHHHHHHHHHC
APGKYNDPDMLVVGKLGPGWGAKSHDSDLTADEQYAHISLWSILSAPLLLGCDMTAIDDF
CCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHCHHHCCCCCCEECCE
TLGLLTNPEVIAVNQDPLVAPATKLTVPNGQIWYKKLYDGSYALGFFQMDPYFILWDQDK
EEEEECCCCEEEECCCCCCCCCEEEECCCCCEEEEEECCCCEEEEEEEECCEEEEECCCC
AVNIQQQKYNFNFALNQLGIQGKVKIRDLWRQKNLGIFSGSYETSIPYHGVSLIKITPIK
CCCEEEEEEEEEEEEECCCCCCEEEHHHHHHHCCCCEEECCCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA