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The map label for this gene is lacZ [H]

Identifier: 29348922

GI number: 29348922

Start: 4533444

End: 4535258

Strand: Reverse

Name: lacZ [H]

Synonym: BT_3513

Alternate gene names: 29348922

Gene position: 4535258-4533444 (Counterclockwise)

Preceding gene: 29348924

Following gene: 29348921

Centisome position: 72.44

GC content: 46.28

Gene sequence:

>1815_bases
ATGAAAAAGAATTTTCTAGCAGTGCTATTCGCATTGGCTCTTTGTAGCCCGACATTCGCTCAATGGAAGCCCGCCGGTGA
TAAGATAAAGACTCCGTGGGGTGAGCAGTTGAATCCTAAAAACGTATTACCGGAATACCCACGCCCCATTATGGAACGCC
AGGATTGGAAGAATCTGAATGGTAGCTGGAATTATGCGATTACTAAAAAAGGAGAACCGGCACCAAACAATTACCAAGGA
GAGATTTTAGTACCTTTTGCAGTCGAATCTTCCCTGTCAGGAGTAGGTAAAACCATTAACGAGCATCAGGAATTATGGTA
TCAGCGTACTTTTGATGTACCGTCAGCATGGAAAGGCAAACAAATATTACTGCACTTCGGAGCTGTGGACTGGAAAGCCG
ATGTATGGGTAAATGACGTAAAAGTAGGACAACATACAGGAGGCTTCACTCCATTCTACTTTGATATTACTTCCGCATTG
AATAAAGGCAATAACCAGTTGGTTGTGAAAGTGTGGGACCCCTCTGACCGTGGCGAACAGCCGAGAGGAAAACAAGTAGA
AAGACCGGAAGGAATCTGGTATACACCAGTCACAGGTATCTGGCAGACTGTATGGCTGGAACCGGTAGCTGCCCAGCATA
TCGCCCAACTGAAAACAACTCCGGATATCGACAAGAAGACTGTCAAAGTGGAAGTAGCCACCAACGTATGCTCTCCAGAC
AAAGTGGAAGTGAAAGTATTCGACGGCAAAAACCTCGTAGCCAAAGGAGCAGCGCTCAATGGCGTACCGGTAGAATTAAC
AATGCCGGAAGACGTTAAATTATGGACACCCGAGTCTCCTTCACTTTATAATATGGAAGTCACTCTTTATAAAGACGGCA
AAGCTGTAGACCAAGTGAAGAGTTACACCGCTCTCCGCAAATTCTCAACTCATAAAGACAAGAATGGTATTACACGCCTG
CAACTCAACAACAAAGACTATTTCCAGTTTGGTCCGCTCGACCAGGGTTGGTGGCCGGACGGCTTATACACAGCGCCAAC
AGACGAAGCATTGGTATATGACTTGAAGAAGATCAAAGATTTCGGCTACAACATGGTGCGCAAGCACGTAAAAGTAGAAC
CGGCACGCTGGTATACTTATTGCGACCAACTCGGACTGATCGTATGGCAAGATATGCCGAACGGAGGTCGCGGTCCTGCC
GAATGGCAGATGCACAAGTACTATGACGGCGCAGACGCCATCCGCTCCGCAGCATCAGAAGCTAACTATCGCAAAGAATG
GAAAGAAATTATCGACTACCTGTATTCTTATCCGAGCATCGGCGTATGGGTACCTTTCAACGAAGCATGGGGACAATTCA
AGACTCCTGAAATCACTAAATGGACGAAAGAATACGACCCCAGCCGTCTGGTAAACCCTGCCAGTGGAGGTAACCACTAC
ACGTGCGGAGATATTCTCGACCTTCATAACTATCCCGGACCGAACCTCTATCTCTACGATCCGATACGCGCTACCGTACT
GGGCGAATATGGCGGTATAGGTATGGCACTGAAAGGACATCTGTGGCTGGCAGACAAGAACTGGGGCTACGTGAAGTTCA
ACACGCCGGAAGAAGTGACTAATGAATACATAAAATATGCAGACCATTTATTGGAACTGATCGAGAAAGGCTTCTCGGCT
GCCGTATACACACAAATCACAGATGTGGAAGAAGAAGTGAACGGTCTGGTTACTTACGACCGGAAAGTTATTAAAGTAGA
CGAGCCTAAAATCAAAGCCATCAACCAGAAAATTTGTAACTCGCTAAATAAATAA

Upstream 100 bases:

>100_bases
AACAGCAAATTTCCTTTATTCTTCTCTGATTAGCTTTTCTTTGCAATATTCCTTTTATGAATATTAATTCTTCTATTTAA
TACATATAAAGATAATTACC

Downstream 100 bases:

>100_bases
AAGTTTCATATTAATGAATAAACTATTCTCTACAATCATGTCAGTTTGCCTCGCATGCAATGTGCAGGCAACTGACCTGT
TTAAAACGAGTCAGGATATC

Product: beta-galactosidase

Products: NA

Alternate protein names: Beta-gal; Lactase [H]

Number of amino acids: Translated: 604; Mature: 604

Protein sequence:

>604_residues
MKKNFLAVLFALALCSPTFAQWKPAGDKIKTPWGEQLNPKNVLPEYPRPIMERQDWKNLNGSWNYAITKKGEPAPNNYQG
EILVPFAVESSLSGVGKTINEHQELWYQRTFDVPSAWKGKQILLHFGAVDWKADVWVNDVKVGQHTGGFTPFYFDITSAL
NKGNNQLVVKVWDPSDRGEQPRGKQVERPEGIWYTPVTGIWQTVWLEPVAAQHIAQLKTTPDIDKKTVKVEVATNVCSPD
KVEVKVFDGKNLVAKGAALNGVPVELTMPEDVKLWTPESPSLYNMEVTLYKDGKAVDQVKSYTALRKFSTHKDKNGITRL
QLNNKDYFQFGPLDQGWWPDGLYTAPTDEALVYDLKKIKDFGYNMVRKHVKVEPARWYTYCDQLGLIVWQDMPNGGRGPA
EWQMHKYYDGADAIRSAASEANYRKEWKEIIDYLYSYPSIGVWVPFNEAWGQFKTPEITKWTKEYDPSRLVNPASGGNHY
TCGDILDLHNYPGPNLYLYDPIRATVLGEYGGIGMALKGHLWLADKNWGYVKFNTPEEVTNEYIKYADHLLELIEKGFSA
AVYTQITDVEEEVNGLVTYDRKVIKVDEPKIKAINQKICNSLNK

Sequences:

>Translated_604_residues
MKKNFLAVLFALALCSPTFAQWKPAGDKIKTPWGEQLNPKNVLPEYPRPIMERQDWKNLNGSWNYAITKKGEPAPNNYQG
EILVPFAVESSLSGVGKTINEHQELWYQRTFDVPSAWKGKQILLHFGAVDWKADVWVNDVKVGQHTGGFTPFYFDITSAL
NKGNNQLVVKVWDPSDRGEQPRGKQVERPEGIWYTPVTGIWQTVWLEPVAAQHIAQLKTTPDIDKKTVKVEVATNVCSPD
KVEVKVFDGKNLVAKGAALNGVPVELTMPEDVKLWTPESPSLYNMEVTLYKDGKAVDQVKSYTALRKFSTHKDKNGITRL
QLNNKDYFQFGPLDQGWWPDGLYTAPTDEALVYDLKKIKDFGYNMVRKHVKVEPARWYTYCDQLGLIVWQDMPNGGRGPA
EWQMHKYYDGADAIRSAASEANYRKEWKEIIDYLYSYPSIGVWVPFNEAWGQFKTPEITKWTKEYDPSRLVNPASGGNHY
TCGDILDLHNYPGPNLYLYDPIRATVLGEYGGIGMALKGHLWLADKNWGYVKFNTPEEVTNEYIKYADHLLELIEKGFSA
AVYTQITDVEEEVNGLVTYDRKVIKVDEPKIKAINQKICNSLNK
>Mature_604_residues
MKKNFLAVLFALALCSPTFAQWKPAGDKIKTPWGEQLNPKNVLPEYPRPIMERQDWKNLNGSWNYAITKKGEPAPNNYQG
EILVPFAVESSLSGVGKTINEHQELWYQRTFDVPSAWKGKQILLHFGAVDWKADVWVNDVKVGQHTGGFTPFYFDITSAL
NKGNNQLVVKVWDPSDRGEQPRGKQVERPEGIWYTPVTGIWQTVWLEPVAAQHIAQLKTTPDIDKKTVKVEVATNVCSPD
KVEVKVFDGKNLVAKGAALNGVPVELTMPEDVKLWTPESPSLYNMEVTLYKDGKAVDQVKSYTALRKFSTHKDKNGITRL
QLNNKDYFQFGPLDQGWWPDGLYTAPTDEALVYDLKKIKDFGYNMVRKHVKVEPARWYTYCDQLGLIVWQDMPNGGRGPA
EWQMHKYYDGADAIRSAASEANYRKEWKEIIDYLYSYPSIGVWVPFNEAWGQFKTPEITKWTKEYDPSRLVNPASGGNHY
TCGDILDLHNYPGPNLYLYDPIRATVLGEYGGIGMALKGHLWLADKNWGYVKFNTPEEVTNEYIKYADHLLELIEKGFSA
AVYTQITDVEEEVNGLVTYDRKVIKVDEPKIKAINQKICNSLNK

Specific function: The Wild-Type Enzyme Is An Ineffective Lactase. Two Classes Of Point Mutations Dramatically Improve Activity Of The Enzyme. [C]

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Escherichia coli, GI48994920, Length=433, Percent_Identity=24.4803695150115, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1787903, Length=264, Percent_Identity=26.1363636363636, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24655438, Length=215, Percent_Identity=27.4418604651163, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19922582, Length=215, Percent_Identity=27.4418604651163, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI62471735, Length=215, Percent_Identity=27.4418604651163, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003344
- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 68823; Mature: 68823

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNFLAVLFALALCSPTFAQWKPAGDKIKTPWGEQLNPKNVLPEYPRPIMERQDWKNLN
CCCHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHCCCC
GSWNYAITKKGEPAPNNYQGEILVPFAVESSLSGVGKTINEHQELWYQRTFDVPSAWKGK
CCEEEEEEECCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
QILLHFGAVDWKADVWVNDVKVGQHTGGFTPFYFDITSALNKGNNQLVVKVWDPSDRGEQ
EEEEEECCCCEEECEEEEEEEECCCCCCCCEEEEEHHHHHCCCCCEEEEEEECCCCCCCC
PRGKQVERPEGIWYTPVTGIWQTVWLEPVAAQHIAQLKTTPDIDKKTVKVEVATNVCSPD
CCCCCCCCCCCEEECCHHHHHHHHHHCHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCC
KVEVKVFDGKNLVAKGAALNGVPVELTMPEDVKLWTPESPSLYNMEVTLYKDGKAVDQVK
EEEEEEECCCCEEECCCCCCCEEEEEECCCCCEEECCCCCCEEEEEEEEEECCCHHHHHH
SYTALRKFSTHKDKNGITRLQLNNKDYFQFGPLDQGWWPDGLYTAPTDEALVYDLKKIKD
HHHHHHHHHCCCCCCCEEEEEECCCCCEEECCCCCCCCCCCCEECCCCCHHHHHHHHHHH
FGYNMVRKHVKVEPARWYTYCDQLGLIVWQDMPNGGRGPAEWQMHKYYDGADAIRSAASE
HCHHHHHHHHCCCCHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHEECCCHHHHHHHHHH
ANYRKEWKEIIDYLYSYPSIGVWVPFNEAWGQFKTPEITKWTKEYDPSRLVNPASGGNHY
HHHHHHHHHHHHHHHCCCCCEEEEECHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCE
TCGDILDLHNYPGPNLYLYDPIRATVLGEYGGIGMALKGHLWLADKNWGYVKFNTPEEVT
ECCCHHHHCCCCCCCEEEECCCEEEEEECCCCCEEEEECEEEEEECCCEEEEECCHHHHH
NEYIKYADHLLELIEKGFSAAVYTQITDVEEEVNGLVTYDRKVIKVDEPKIKAINQKICN
HHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCEEEECEEEEEECCCHHHHHHHHHHH
SLNK
HHCC
>Mature Secondary Structure
MKKNFLAVLFALALCSPTFAQWKPAGDKIKTPWGEQLNPKNVLPEYPRPIMERQDWKNLN
CCCHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHCCCC
GSWNYAITKKGEPAPNNYQGEILVPFAVESSLSGVGKTINEHQELWYQRTFDVPSAWKGK
CCEEEEEEECCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
QILLHFGAVDWKADVWVNDVKVGQHTGGFTPFYFDITSALNKGNNQLVVKVWDPSDRGEQ
EEEEEECCCCEEECEEEEEEEECCCCCCCCEEEEEHHHHHCCCCCEEEEEEECCCCCCCC
PRGKQVERPEGIWYTPVTGIWQTVWLEPVAAQHIAQLKTTPDIDKKTVKVEVATNVCSPD
CCCCCCCCCCCEEECCHHHHHHHHHHCHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCC
KVEVKVFDGKNLVAKGAALNGVPVELTMPEDVKLWTPESPSLYNMEVTLYKDGKAVDQVK
EEEEEEECCCCEEECCCCCCCEEEEEECCCCCEEECCCCCCEEEEEEEEEECCCHHHHHH
SYTALRKFSTHKDKNGITRLQLNNKDYFQFGPLDQGWWPDGLYTAPTDEALVYDLKKIKD
HHHHHHHHHCCCCCCCEEEEEECCCCCEEECCCCCCCCCCCCEECCCCCHHHHHHHHHHH
FGYNMVRKHVKVEPARWYTYCDQLGLIVWQDMPNGGRGPAEWQMHKYYDGADAIRSAASE
HCHHHHHHHHCCCCHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHEECCCHHHHHHHHHH
ANYRKEWKEIIDYLYSYPSIGVWVPFNEAWGQFKTPEITKWTKEYDPSRLVNPASGGNHY
HHHHHHHHHHHHHHHCCCCCEEEEECHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCE
TCGDILDLHNYPGPNLYLYDPIRATVLGEYGGIGMALKGHLWLADKNWGYVKFNTPEEVT
ECCCHHHHCCCCCCCEEEECCCEEEEEECCCCCEEEEECEEEEEECCCEEEEECCHHHHH
NEYIKYADHLLELIEKGFSAAVYTQITDVEEEVNGLVTYDRKVIKVDEPKIKAINQKICN
HHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCEEEECEEEEEECCCHHHHHHHHHHH
SLNK
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA