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The map label for this gene is cysS

Identifier: 29348760

GI number: 29348760

Start: 4322214

End: 4323695

Strand: Reverse

Name: cysS

Synonym: BT_3351

Alternate gene names: 29348760

Gene position: 4323695-4322214 (Counterclockwise)

Preceding gene: 29348761

Following gene: 29348756

Centisome position: 69.06

GC content: 47.57

Gene sequence:

>1482_bases
ATGGAACATCAACTTACCATTTACAACACGTTAGATAGAAAGAAAGAATTGTTCGTACCGCTTCACGCACCGCATGTAGG
CATGTATGTATGCGGACCGACAGTATACGGTGATGCTCACTTGGGACATGCCCGTCCCGCCATCACATTTGACGTACTCT
TCCGTTATCTGACTCATTTAGGATATAAAGTACGCTATGTACGCAATATTACCGATGTCGGTCATCTGGAGCATGATGCT
GACGAAGGAGAAGACAAAATAGCCAAGAAAGCCCGTCTCGAAGAATTGGAGCCTATGGAAGTTGTTCAGTATTACCTGAA
CCGCTATCATAAGGCAATGGAAGCACTGAATGTACTTTCTCCCAGCATCGAACCGCACGCTTCGGGTCACATTATCGAAC
AAATCCAGCTCGTACAGAAGATTCTGGACGCCGGATACGCTTACGAAAGCGAAGGTTCCGTCTACTTTGACGTAGCTAAA
TATAACAAGGATTATCATTACGGCAAACTCTCAGGGCGCAACTTGGACGACGTACTGAATACCACCCGCGACCTCGATGG
ACAAAGCGAAAAACGTAATCCCGCCGACTTCGCCCTCTGGAAAAAAGCGCAGCCGGAACACATCATGCGCTGGCCTTCTC
CATGGAGCGACGGTTTCCCCGGATGGCATGCCGAATGTACCGCCATGGGACGCAAGTATCTGGGCGAGCACTTCGATATT
CACGGAGGAGGAATGGACCTGATCTTCCCGCATCATGAATGTGAGATCGCACAGTCGGTAGCTTCGCAAGGCGATGACAT
GGTTCACTACTGGATGCACAACAACATGATTACCATCAACGGTACGAAGATGGGTAAATCACTCGGCAACTTCATCACGC
TGGATGAATTCTTCAACGGTACTCACAAGTTGCTGGCACAAGCCTACACTCCGATGACCATCCGTTTCTTCATCCTGCAA
GCTCACTATCGCAGCACTGTAGACTTCAGTAACGAAGCATTGCAAGCTTCGGAAAAAGGGCTGCAACGTTTGATAGAAGC
AATCGAAGGACTCGACAAGATCACTCCGGCCGCAACAACTTCGGAAGGTATCAACGTGAAAGAGCTGCGTGCTAAATGCT
ACGAAGCTATGAATGACGACTTGAATACTCCTATTGTCATCGCACAGCTTTTCGAAGGTGCCCGCATCATCAATAATATC
AACGCAGGAAATGCAACGATCTCCGCAGAAGATCTGAAAGACCTGAAAGAAACTTTCCACCTGTTCTGCTTCGACATCAT
GGGGCTGAAAGAAGAGAAAGGTTCTTCCGACGGTCGCGAAGCCGCTTATGGCAAAGTCGTTGATATGCTTCTTGAACAAC
GCATGAAAGCAAAAGCCAACAAAGACTGGGCTACTTCCGACGAAATCCGTAATACATTGACTGCACTCGGATTTGAAGTG
AAAGATACAAAAGATGGTTTCGAATGGAGATTAAACAAGTAA

Upstream 100 bases:

>100_bases
ATTCTTCCTTCAGCAGATCAGACAAAATCCCTTGCAAGTTAAGTTGAAATCAATTATTTATTCTAACTTTGCACTTCCAA
TAGAACAAATATAAAATGTT

Downstream 100 bases:

>100_bases
TTCAAGTTCTCCCTAGCAAACTGTCTCATTAACCGCATATAAAACTGAGGGTCGATTCTTCGCTGAACCGACCCTCTTTC
AAGGTATGAAACACAAACAT

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MEHQLTIYNTLDRKKELFVPLHAPHVGMYVCGPTVYGDAHLGHARPAITFDVLFRYLTHLGYKVRYVRNITDVGHLEHDA
DEGEDKIAKKARLEELEPMEVVQYYLNRYHKAMEALNVLSPSIEPHASGHIIEQIQLVQKILDAGYAYESEGSVYFDVAK
YNKDYHYGKLSGRNLDDVLNTTRDLDGQSEKRNPADFALWKKAQPEHIMRWPSPWSDGFPGWHAECTAMGRKYLGEHFDI
HGGGMDLIFPHHECEIAQSVASQGDDMVHYWMHNNMITINGTKMGKSLGNFITLDEFFNGTHKLLAQAYTPMTIRFFILQ
AHYRSTVDFSNEALQASEKGLQRLIEAIEGLDKITPAATTSEGINVKELRAKCYEAMNDDLNTPIVIAQLFEGARIINNI
NAGNATISAEDLKDLKETFHLFCFDIMGLKEEKGSSDGREAAYGKVVDMLLEQRMKAKANKDWATSDEIRNTLTALGFEV
KDTKDGFEWRLNK

Sequences:

>Translated_493_residues
MEHQLTIYNTLDRKKELFVPLHAPHVGMYVCGPTVYGDAHLGHARPAITFDVLFRYLTHLGYKVRYVRNITDVGHLEHDA
DEGEDKIAKKARLEELEPMEVVQYYLNRYHKAMEALNVLSPSIEPHASGHIIEQIQLVQKILDAGYAYESEGSVYFDVAK
YNKDYHYGKLSGRNLDDVLNTTRDLDGQSEKRNPADFALWKKAQPEHIMRWPSPWSDGFPGWHAECTAMGRKYLGEHFDI
HGGGMDLIFPHHECEIAQSVASQGDDMVHYWMHNNMITINGTKMGKSLGNFITLDEFFNGTHKLLAQAYTPMTIRFFILQ
AHYRSTVDFSNEALQASEKGLQRLIEAIEGLDKITPAATTSEGINVKELRAKCYEAMNDDLNTPIVIAQLFEGARIINNI
NAGNATISAEDLKDLKETFHLFCFDIMGLKEEKGSSDGREAAYGKVVDMLLEQRMKAKANKDWATSDEIRNTLTALGFEV
KDTKDGFEWRLNK
>Mature_493_residues
MEHQLTIYNTLDRKKELFVPLHAPHVGMYVCGPTVYGDAHLGHARPAITFDVLFRYLTHLGYKVRYVRNITDVGHLEHDA
DEGEDKIAKKARLEELEPMEVVQYYLNRYHKAMEALNVLSPSIEPHASGHIIEQIQLVQKILDAGYAYESEGSVYFDVAK
YNKDYHYGKLSGRNLDDVLNTTRDLDGQSEKRNPADFALWKKAQPEHIMRWPSPWSDGFPGWHAECTAMGRKYLGEHFDI
HGGGMDLIFPHHECEIAQSVASQGDDMVHYWMHNNMITINGTKMGKSLGNFITLDEFFNGTHKLLAQAYTPMTIRFFILQ
AHYRSTVDFSNEALQASEKGLQRLIEAIEGLDKITPAATTSEGINVKELRAKCYEAMNDDLNTPIVIAQLFEGARIINNI
NAGNATISAEDLKDLKETFHLFCFDIMGLKEEKGSSDGREAAYGKVVDMLLEQRMKAKANKDWATSDEIRNTLTALGFEV
KDTKDGFEWRLNK

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=521, Percent_Identity=31.4779270633397, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI21269877, Length=245, Percent_Identity=40.4081632653061, Blast_Score=186, Evalue=4e-47,
Organism=Homo sapiens, GI10835051, Length=245, Percent_Identity=41.2244897959184, Blast_Score=186, Evalue=4e-47,
Organism=Homo sapiens, GI62240992, Length=248, Percent_Identity=39.9193548387097, Blast_Score=186, Evalue=5e-47,
Organism=Homo sapiens, GI303304987, Length=363, Percent_Identity=32.7823691460055, Blast_Score=186, Evalue=5e-47,
Organism=Escherichia coli, GI1786737, Length=491, Percent_Identity=41.1405295315682, Blast_Score=345, Evalue=3e-96,
Organism=Caenorhabditis elegans, GI17509797, Length=340, Percent_Identity=32.0588235294118, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17509795, Length=340, Percent_Identity=32.0588235294118, Blast_Score=157, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6324082, Length=369, Percent_Identity=32.520325203252, Blast_Score=168, Evalue=2e-42,
Organism=Drosophila melanogaster, GI19922218, Length=523, Percent_Identity=31.9311663479924, Blast_Score=209, Evalue=3e-54,
Organism=Drosophila melanogaster, GI19922364, Length=245, Percent_Identity=37.1428571428571, Blast_Score=166, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_BACTN (Q8A2F4)

Other databases:

- EMBL:   AE015928
- RefSeq:   NP_812263.1
- ProteinModelPortal:   Q8A2F4
- SMR:   Q8A2F4
- GeneID:   1075333
- GenomeReviews:   AE015928_GR
- KEGG:   bth:BT_3351
- NMPDR:   fig|226186.1.peg.3350
- HOGENOM:   HBG327651
- OMA:   PEEMLAG
- ProtClustDB:   PRK00260
- BioCyc:   BTHE226186:BT_3351-MONOMER
- BRENDA:   6.1.1.16
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 55997; Mature: 55997

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 286-286

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHQLTIYNTLDRKKELFVPLHAPHVGMYVCGPTVYGDAHLGHARPAITFDVLFRYLTHL
CCCCEEEHHHHCCCCCEEEEECCCCCCEEEECCEEECCCCCCCCCCHHHHHHHHHHHHHC
GYKVRYVRNITDVGHLEHDADEGEDKIAKKARLEELEPMEVVQYYLNRYHKAMEALNVLS
CCEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC
PSIEPHASGHIIEQIQLVQKILDAGYAYESEGSVYFDVAKYNKDYHYGKLSGRNLDDVLN
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHH
TTRDLDGQSEKRNPADFALWKKAQPEHIMRWPSPWSDGFPGWHAECTAMGRKYLGEHFDI
HHHCCCCCCCCCCCCHHHHHCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEE
HGGGMDLIFPHHECEIAQSVASQGDDMVHYWMHNNMITINGTKMGKSLGNFITLDEFFNG
CCCCEEEECCCHHHHHHHHHHHCCCCEEEEEEECCEEEEECHHHHHHHCCEEEHHHHHCH
THKLLAQAYTPMTIRFFILQAHYRSTVDFSNEALQASEKGLQRLIEAIEGLDKITPAATT
HHHHHHHHCCCCEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SEGINVKELRAKCYEAMNDDLNTPIVIAQLFEGARIINNINAGNATISAEDLKDLKETFH
CCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHCCCCCCCEEEHHHHHHHHHHHH
LFCFDIMGLKEEKGSSDGREAAYGKVVDMLLEQRMKAKANKDWATSDEIRNTLTALGFEV
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCEE
KDTKDGFEWRLNK
CCCCCCCCEECCC
>Mature Secondary Structure
MEHQLTIYNTLDRKKELFVPLHAPHVGMYVCGPTVYGDAHLGHARPAITFDVLFRYLTHL
CCCCEEEHHHHCCCCCEEEEECCCCCCEEEECCEEECCCCCCCCCCHHHHHHHHHHHHHC
GYKVRYVRNITDVGHLEHDADEGEDKIAKKARLEELEPMEVVQYYLNRYHKAMEALNVLS
CCEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC
PSIEPHASGHIIEQIQLVQKILDAGYAYESEGSVYFDVAKYNKDYHYGKLSGRNLDDVLN
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHH
TTRDLDGQSEKRNPADFALWKKAQPEHIMRWPSPWSDGFPGWHAECTAMGRKYLGEHFDI
HHHCCCCCCCCCCCCHHHHHCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEE
HGGGMDLIFPHHECEIAQSVASQGDDMVHYWMHNNMITINGTKMGKSLGNFITLDEFFNG
CCCCEEEECCCHHHHHHHHHHHCCCCEEEEEEECCEEEEECHHHHHHHCCEEEHHHHHCH
THKLLAQAYTPMTIRFFILQAHYRSTVDFSNEALQASEKGLQRLIEAIEGLDKITPAATT
HHHHHHHHCCCCEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SEGINVKELRAKCYEAMNDDLNTPIVIAQLFEGARIINNINAGNATISAEDLKDLKETFH
CCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHCCCCCCCEEEHHHHHHHHHHHH
LFCFDIMGLKEEKGSSDGREAAYGKVVDMLLEQRMKAKANKDWATSDEIRNTLTALGFEV
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCEE
KDTKDGFEWRLNK
CCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663928