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The map label for this gene is nadB [H]

Identifier: 29348593

GI number: 29348593

Start: 4072734

End: 4074305

Strand: Reverse

Name: nadB [H]

Synonym: BT_3184

Alternate gene names: 29348593

Gene position: 4074305-4072734 (Counterclockwise)

Preceding gene: 29348601

Following gene: 29348592

Centisome position: 65.08

GC content: 50.57

Gene sequence:

>1572_bases
ATGGTAAGAAAATTCGATTTCCTCGTTATCGGCTCCGGAATTGCCGGTATGAGTTTTGCGCTCAAAGTGGCGCACAAAGG
TAAAGTTGCTCTTATCTGTAAAAGCGGGCTGGAAGAAGCCAATACGTACTTCGCACAAGGAGGAGTGGCGTCCGTCACCA
ACCTGCTGGTGGATAATTTCGACAAACACATTGAAGACACGATGATTGCCGGCGACTGGATCAGTGACCGTGGCGCAGTA
GAAAAAGTAGTGCGCGAAGCGCCTGCGCAAATTGAAGAACTGATCAAATGGGGCGTGGACTTCGACAAAAACGAGAAAGG
CGAATTTGACTTGCACCGCGAGGGAGGACACTCCGAGTTCCGCATTCTTCACCATAAAGATAATACGGGAGCAGAGATTC
AGGACAGCCTGATCAAAGCCGTGCAAAGGCATCCGAACATCGAGGTGATCGAAAACCAGTTTGCCGTTGAAATACTGACC
CAACATCATCTGGGAGTAACTGTTACCCGCCAGACACCGGACATCAAATGCTACGGTGCCTACATCCTCGACCCGAAAAC
CGGAAAAGTAGATACCTACCTCGCCAAAGTGACTCTTATGGCCACCGGTGGAGTAGGAGCTGTTTATAAAACGACCACCA
ATCCGCTAGTCGCTACCGGAGACGGTATCGCCATGGTCTACCGTGCCAAAGGTACAGTGAAGGATATGGAGTTTGTGCAA
TTCCACCCCACCGCCCTGTACCATCCGGGCGACCGCCCTTCGTTCCTGATTACGGAAGCGATGCGTGGCTACGGTGGCGT
ACTCCGCACAATGGACGGCAAGGAGTTCATGCAAAAATACGATCCCCGTCTTTCACTCGCTCCGCGCGATATCGTAGCCC
GTGCTATTGACAACGAAATGAAAAATCGGGGTGATGACCATGTTTATCTTGACGTCACCCACAAAGACCCGGAAGAGACA
AAGAAGCACTTTCCCAATATTTACGAGAAATGCCTCAGCTTGGGCATCGACATCACCAAAGATTACATTCCGGTAGCTCC
GGCAGCCCACTATCTTTGCGGCGGTATCCTTGTAGACCTGAACGGACAGTCTTCTATCGAACGCCTCTACGCAGTGGGAG
AGTGCTCCTGCACCGGTCTCCACGGTGGTAACCGTCTGGCTTCCAACTCACTGATCGAAGCTGTCGTATATGCCGACGCT
GCCGCCAAGCATAGCCTGAGAGTTGTAGACCAGTATGCCTACAACGAAGCCATCCCCGAATGGAACGACGAGGGAACCCG
TTCACCGGAAGAGATGGTACTGATCACGCAAAGCATGAAGGAAGTTAACCAGATTATGAGTACTTATGTAGGTATTGTCC
GCAGCGACCTTCGTCTGAAACGTGCCTGGGACCGACTGGATATCATTTACGAAGAAACTGAAAGCCTGTTCAAACGCAGC
GTAGCCTCCAGAGAGATTTGCGAATTGCGTAATATGGTCAACGTAGGATATCTGATTATGCGTCAGGCAATGGAACGCAA
AGAAAGCCGCGGATTGCATTATACGATTGATTATCCACACGCTAAAAAATAA

Upstream 100 bases:

>100_bases
GATTTTTGCGTGCAAGGTAGCAAATTTATGAATTAGTGACAACGAAAAGAACAACGAAGTGACTTATTTTGTAGCTTTGC
GGTGAGAAATAAACAAAATT

Downstream 100 bases:

>100_bases
TCATGAAAACATTAACTAGACTATTTATTCTTTTATTCGTTATGGCAGGTGTAAGTTCCTGCTATACAGGTATCCCATTG
AAACAAGGCAAATCGGAAAA

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 523; Mature: 523

Protein sequence:

>523_residues
MVRKFDFLVIGSGIAGMSFALKVAHKGKVALICKSGLEEANTYFAQGGVASVTNLLVDNFDKHIEDTMIAGDWISDRGAV
EKVVREAPAQIEELIKWGVDFDKNEKGEFDLHREGGHSEFRILHHKDNTGAEIQDSLIKAVQRHPNIEVIENQFAVEILT
QHHLGVTVTRQTPDIKCYGAYILDPKTGKVDTYLAKVTLMATGGVGAVYKTTTNPLVATGDGIAMVYRAKGTVKDMEFVQ
FHPTALYHPGDRPSFLITEAMRGYGGVLRTMDGKEFMQKYDPRLSLAPRDIVARAIDNEMKNRGDDHVYLDVTHKDPEET
KKHFPNIYEKCLSLGIDITKDYIPVAPAAHYLCGGILVDLNGQSSIERLYAVGECSCTGLHGGNRLASNSLIEAVVYADA
AAKHSLRVVDQYAYNEAIPEWNDEGTRSPEEMVLITQSMKEVNQIMSTYVGIVRSDLRLKRAWDRLDIIYEETESLFKRS
VASREICELRNMVNVGYLIMRQAMERKESRGLHYTIDYPHAKK

Sequences:

>Translated_523_residues
MVRKFDFLVIGSGIAGMSFALKVAHKGKVALICKSGLEEANTYFAQGGVASVTNLLVDNFDKHIEDTMIAGDWISDRGAV
EKVVREAPAQIEELIKWGVDFDKNEKGEFDLHREGGHSEFRILHHKDNTGAEIQDSLIKAVQRHPNIEVIENQFAVEILT
QHHLGVTVTRQTPDIKCYGAYILDPKTGKVDTYLAKVTLMATGGVGAVYKTTTNPLVATGDGIAMVYRAKGTVKDMEFVQ
FHPTALYHPGDRPSFLITEAMRGYGGVLRTMDGKEFMQKYDPRLSLAPRDIVARAIDNEMKNRGDDHVYLDVTHKDPEET
KKHFPNIYEKCLSLGIDITKDYIPVAPAAHYLCGGILVDLNGQSSIERLYAVGECSCTGLHGGNRLASNSLIEAVVYADA
AAKHSLRVVDQYAYNEAIPEWNDEGTRSPEEMVLITQSMKEVNQIMSTYVGIVRSDLRLKRAWDRLDIIYEETESLFKRS
VASREICELRNMVNVGYLIMRQAMERKESRGLHYTIDYPHAKK
>Mature_523_residues
MVRKFDFLVIGSGIAGMSFALKVAHKGKVALICKSGLEEANTYFAQGGVASVTNLLVDNFDKHIEDTMIAGDWISDRGAV
EKVVREAPAQIEELIKWGVDFDKNEKGEFDLHREGGHSEFRILHHKDNTGAEIQDSLIKAVQRHPNIEVIENQFAVEILT
QHHLGVTVTRQTPDIKCYGAYILDPKTGKVDTYLAKVTLMATGGVGAVYKTTTNPLVATGDGIAMVYRAKGTVKDMEFVQ
FHPTALYHPGDRPSFLITEAMRGYGGVLRTMDGKEFMQKYDPRLSLAPRDIVARAIDNEMKNRGDDHVYLDVTHKDPEET
KKHFPNIYEKCLSLGIDITKDYIPVAPAAHYLCGGILVDLNGQSSIERLYAVGECSCTGLHGGNRLASNSLIEAVVYADA
AAKHSLRVVDQYAYNEAIPEWNDEGTRSPEEMVLITQSMKEVNQIMSTYVGIVRSDLRLKRAWDRLDIIYEETESLFKRS
VASREICELRNMVNVGYLIMRQAMERKESRGLHYTIDYPHAKK

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=530, Percent_Identity=32.0754716981132, Blast_Score=229, Evalue=6e-60,
Organism=Escherichia coli, GI1788928, Length=519, Percent_Identity=46.8208092485549, Blast_Score=466, Evalue=1e-132,
Organism=Escherichia coli, GI1790597, Length=527, Percent_Identity=32.4478178368121, Blast_Score=231, Evalue=6e-62,
Organism=Escherichia coli, GI1786942, Length=560, Percent_Identity=31.9642857142857, Blast_Score=212, Evalue=5e-56,
Organism=Caenorhabditis elegans, GI17505833, Length=535, Percent_Identity=32.1495327102804, Blast_Score=231, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI17550100, Length=543, Percent_Identity=30.5709023941068, Blast_Score=218, Evalue=7e-57,
Organism=Caenorhabditis elegans, GI71986328, Length=466, Percent_Identity=26.1802575107296, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6322701, Length=562, Percent_Identity=32.9181494661922, Blast_Score=241, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6322416, Length=556, Percent_Identity=30.9352517985612, Blast_Score=220, Evalue=4e-58,
Organism=Saccharomyces cerevisiae, GI6320788, Length=485, Percent_Identity=25.360824742268, Blast_Score=91, Evalue=5e-19,
Organism=Drosophila melanogaster, GI17137288, Length=535, Percent_Identity=30.4672897196262, Blast_Score=218, Evalue=7e-57,
Organism=Drosophila melanogaster, GI24655642, Length=535, Percent_Identity=30.4672897196262, Blast_Score=218, Evalue=7e-57,
Organism=Drosophila melanogaster, GI24655647, Length=535, Percent_Identity=30.4672897196262, Blast_Score=218, Evalue=7e-57,
Organism=Drosophila melanogaster, GI24663005, Length=559, Percent_Identity=30.2325581395349, Blast_Score=211, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 58533; Mature: 58533

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRKFDFLVIGSGIAGMSFALKVAHKGKVALICKSGLEEANTYFAQGGVASVTNLLVDNF
CCEEEEEEEEECCCCCHHHEEEEECCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHH
DKHIEDTMIAGDWISDRGAVEKVVREAPAQIEELIKWGVDFDKNEKGEFDLHREGGHSEF
HHHHHHHEEECCCCCCCHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCEE
RILHHKDNTGAEIQDSLIKAVQRHPNIEVIENQFAVEILTQHHLGVTVTRQTPDIKCYGA
EEEEECCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCEEEECCCCCEEEEEE
YILDPKTGKVDTYLAKVTLMATGGVGAVYKTTTNPLVATGDGIAMVYRAKGTVKDMEFVQ
EEECCCCCCHHHEEEEEEEEEECCCCEEEEECCCCEEEECCCEEEEEECCCCCCCCCEEE
FHPTALYHPGDRPSFLITEAMRGYGGVLRTMDGKEFMQKYDPRLSLAPRDIVARAIDNEM
ECCCEEECCCCCCCHHHHHHHHHCCCCEECCCHHHHHHHCCCCCCCCHHHHHHHHHHHHH
KNRGDDHVYLDVTHKDPEETKKHFPNIYEKCLSLGIDITKDYIPVAPAAHYLCGGILVDL
HCCCCCEEEEEEECCCHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCEEEEE
NGQSSIERLYAVGECSCTGLHGGNRLASNSLIEAVVYADAAAKHSLRVVDQYAYNEAIPE
CCCHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
WNDEGTRSPEEMVLITQSMKEVNQIMSTYVGIVRSDLRLKRAWDRLDIIYEETESLFKRS
CCCCCCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VASREICELRNMVNVGYLIMRQAMERKESRGLHYTIDYPHAKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCC
>Mature Secondary Structure
MVRKFDFLVIGSGIAGMSFALKVAHKGKVALICKSGLEEANTYFAQGGVASVTNLLVDNF
CCEEEEEEEEECCCCCHHHEEEEECCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHH
DKHIEDTMIAGDWISDRGAVEKVVREAPAQIEELIKWGVDFDKNEKGEFDLHREGGHSEF
HHHHHHHEEECCCCCCCHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCEE
RILHHKDNTGAEIQDSLIKAVQRHPNIEVIENQFAVEILTQHHLGVTVTRQTPDIKCYGA
EEEEECCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCEEEECCCCCEEEEEE
YILDPKTGKVDTYLAKVTLMATGGVGAVYKTTTNPLVATGDGIAMVYRAKGTVKDMEFVQ
EEECCCCCCHHHEEEEEEEEEECCCCEEEEECCCCEEEECCCEEEEEECCCCCCCCCEEE
FHPTALYHPGDRPSFLITEAMRGYGGVLRTMDGKEFMQKYDPRLSLAPRDIVARAIDNEM
ECCCEEECCCCCCCHHHHHHHHHCCCCEECCCHHHHHHHCCCCCCCCHHHHHHHHHHHHH
KNRGDDHVYLDVTHKDPEETKKHFPNIYEKCLSLGIDITKDYIPVAPAAHYLCGGILVDL
HCCCCCEEEEEEECCCHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCEEEEE
NGQSSIERLYAVGECSCTGLHGGNRLASNSLIEAVVYADAAAKHSLRVVDQYAYNEAIPE
CCCHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
WNDEGTRSPEEMVLITQSMKEVNQIMSTYVGIVRSDLRLKRAWDRLDIIYEETESLFKRS
CCCCCCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VASREICELRNMVNVGYLIMRQAMERKESRGLHYTIDYPHAKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7737518; 10984043; 7961421 [H]