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The map label for this gene is cysK [H]

Identifier: 29348489

GI number: 29348489

Start: 3905175

End: 3906122

Strand: Reverse

Name: cysK [H]

Synonym: BT_3080

Alternate gene names: 29348489

Gene position: 3906122-3905175 (Counterclockwise)

Preceding gene: 29348490

Following gene: 29348488

Centisome position: 62.39

GC content: 51.05

Gene sequence:

>948_bases
ATGGAAAAGATTGCAAGGAAATTGACGGATTTGGTAGGTAACACTCCGTTGTTGGAACTAAGTAATTATAATAAGAGTAA
CGGCTTGAAGGCTCGTCTGATCGTGAAAATCGAATCATTCAATCCCGCGGGCAGTGTAAAAGACCGCGTGGCATTGGCAA
TGATTGAAGATGCCGAAATGAAAGGCGTCTTGACTCCCGGCGCAACGATCATCGAGCCTACCAGTGGAAATACGGGAGTA
GGATTGGCATTCGTTGCTGCCGCCAAGGGGTACAAACTGATTTTGACGATGCCCGATACGATGAGCGTCGAACGTCGTAA
CCTTTTGAAAGCTCTGGGAGCGGAACTTGTGCTCACTCCCGGTGCCGACGGAATGAAAGGGGCGATTGCCAAAGCGGAAG
AGTTGAAAGCTGCCACTCCGGGCAGCGTTATCTTACAGCAATTTGAAAATCCTGCTAATCCCGCTATGCATCTACGCACT
ACCGGACTGGAAATATGGAGAGATACCGAGGGGAAAGTCGATATCTTCGTTGCCGGTGTAGGTACTGGCGGGACAATTAG
CGGAGTAGGAGAAGCATTGAAGATGCGCGATCCCAGTGTGAAGGCGGTGGCGGTAGAACCTGCCGACTCTCCGGTACTGT
CCGGCGGAAAGCCCGGACCTCACAAGATTCAGGGAATCGGCGCCGGTTTCGTACCGAAGACTTATAATGCTTCTGTCGTA
GATGAAATTATTCAGGTGCAGAATGACGACGCTATCCGTACCAGTCGTGCGCTGGCGGAGGAAGAAGGGCTGTTGGTCGG
CATATCTTCGGGAGCAGCCGTCTATGCGGCTACCGAACTGGCGAAACGTCCCGAAAACGAAGGGAAGATGATCGTAGCCT
TATTGCCCGATACGGGAGAGCGTTATTTATCTACTATCCTGTACGCGTTTGAAGAGTATCCTTTGTAA

Upstream 100 bases:

>100_bases
AATGATGGTATAAATTTACCACATATATGGTATTTCCTGTCAACAAAAACTGCACTATCTTTGTAATATCTTAAAAACAA
AGGAAATTAAAAACAGATAT

Downstream 100 bases:

>100_bases
AGGAACGTTTTTTTGAAAGAAATATAGAGAATAATCCTGTGGTTACTTGGCAAAACCGTATATTTGCCAAGTAACCATTT
TTATTTAATACTATGCAAAA

Product: cysteine synthase A

Products: NA

Alternate protein names: OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MEKIARKLTDLVGNTPLLELSNYNKSNGLKARLIVKIESFNPAGSVKDRVALAMIEDAEMKGVLTPGATIIEPTSGNTGV
GLAFVAAAKGYKLILTMPDTMSVERRNLLKALGAELVLTPGADGMKGAIAKAEELKAATPGSVILQQFENPANPAMHLRT
TGLEIWRDTEGKVDIFVAGVGTGGTISGVGEALKMRDPSVKAVAVEPADSPVLSGGKPGPHKIQGIGAGFVPKTYNASVV
DEIIQVQNDDAIRTSRALAEEEGLLVGISSGAAVYAATELAKRPENEGKMIVALLPDTGERYLSTILYAFEEYPL

Sequences:

>Translated_315_residues
MEKIARKLTDLVGNTPLLELSNYNKSNGLKARLIVKIESFNPAGSVKDRVALAMIEDAEMKGVLTPGATIIEPTSGNTGV
GLAFVAAAKGYKLILTMPDTMSVERRNLLKALGAELVLTPGADGMKGAIAKAEELKAATPGSVILQQFENPANPAMHLRT
TGLEIWRDTEGKVDIFVAGVGTGGTISGVGEALKMRDPSVKAVAVEPADSPVLSGGKPGPHKIQGIGAGFVPKTYNASVV
DEIIQVQNDDAIRTSRALAEEEGLLVGISSGAAVYAATELAKRPENEGKMIVALLPDTGERYLSTILYAFEEYPL
>Mature_315_residues
MEKIARKLTDLVGNTPLLELSNYNKSNGLKARLIVKIESFNPAGSVKDRVALAMIEDAEMKGVLTPGATIIEPTSGNTGV
GLAFVAAAKGYKLILTMPDTMSVERRNLLKALGAELVLTPGADGMKGAIAKAEELKAATPGSVILQQFENPANPAMHLRT
TGLEIWRDTEGKVDIFVAGVGTGGTISGVGEALKMRDPSVKAVAVEPADSPVLSGGKPGPHKIQGIGAGFVPKTYNASVV
DEIIQVQNDDAIRTSRALAEEEGLLVGISSGAAVYAATELAKRPENEGKMIVALLPDTGERYLSTILYAFEEYPL

Specific function: Catalyzes the conversion of O-acetylserine (OAS) to cysteine through the elimination of acetate and addition of hydrogen sulfide [H]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=313, Percent_Identity=41.5335463258786, Blast_Score=242, Evalue=3e-64,
Organism=Homo sapiens, GI295821200, Length=313, Percent_Identity=41.5335463258786, Blast_Score=242, Evalue=3e-64,
Organism=Homo sapiens, GI4557415, Length=313, Percent_Identity=41.5335463258786, Blast_Score=242, Evalue=3e-64,
Organism=Escherichia coli, GI1788754, Length=318, Percent_Identity=56.2893081761006, Blast_Score=312, Evalue=2e-86,
Organism=Escherichia coli, GI2367138, Length=306, Percent_Identity=42.8104575163399, Blast_Score=236, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI17535051, Length=301, Percent_Identity=55.1495016611296, Blast_Score=328, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI17562970, Length=301, Percent_Identity=53.4883720930233, Blast_Score=316, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI32566674, Length=295, Percent_Identity=53.8983050847458, Blast_Score=311, Evalue=4e-85,
Organism=Caenorhabditis elegans, GI115535073, Length=299, Percent_Identity=57.190635451505, Blast_Score=306, Evalue=9e-84,
Organism=Caenorhabditis elegans, GI17561720, Length=301, Percent_Identity=51.1627906976744, Blast_Score=277, Evalue=6e-75,
Organism=Caenorhabditis elegans, GI32566672, Length=213, Percent_Identity=54.9295774647887, Blast_Score=233, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI17534315, Length=313, Percent_Identity=40.8945686900958, Blast_Score=195, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI25147552, Length=309, Percent_Identity=38.5113268608414, Blast_Score=181, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI17561716, Length=117, Percent_Identity=48.7179487179487, Blast_Score=111, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI71996324, Length=341, Percent_Identity=24.9266862170088, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6321594, Length=319, Percent_Identity=40.4388714733542, Blast_Score=222, Evalue=7e-59,
Organism=Saccharomyces cerevisiae, GI6321449, Length=326, Percent_Identity=35.8895705521472, Blast_Score=144, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24643623, Length=314, Percent_Identity=40.4458598726115, Blast_Score=221, Evalue=4e-58,
Organism=Drosophila melanogaster, GI20129101, Length=314, Percent_Identity=40.4458598726115, Blast_Score=221, Evalue=4e-58,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 33095; Mature: 33095

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKIARKLTDLVGNTPLLELSNYNKSNGLKARLIVKIESFNPAGSVKDRVALAMIEDAEM
CHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEEEECCCCCCCCHHHEEEEEEECCCC
KGVLTPGATIIEPTSGNTGVGLAFVAAAKGYKLILTMPDTMSVERRNLLKALGAELVLTP
CEEECCCCEEEECCCCCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEC
GADGMKGAIAKAEELKAATPGSVILQQFENPANPAMHLRTTGLEIWRDTEGKVDIFVAGV
CCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEEEECCEEEEECCCCCEEEEEEEC
GTGGTISGVGEALKMRDPSVKAVAVEPADSPVLSGGKPGPHKIQGIGAGFVPKTYNASVV
CCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHH
DEIIQVQNDDAIRTSRALAEEEGLLVGISSGAAVYAATELAKRPENEGKMIVALLPDTGE
HHHHHCCCCCHHHHHHHHHHCCCEEEEECCCCEEEEHHHHHHCCCCCCCEEEEECCCCCH
RYLSTILYAFEEYPL
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKIARKLTDLVGNTPLLELSNYNKSNGLKARLIVKIESFNPAGSVKDRVALAMIEDAEM
CHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEEEECCCCCCCCHHHEEEEEEECCCC
KGVLTPGATIIEPTSGNTGVGLAFVAAAKGYKLILTMPDTMSVERRNLLKALGAELVLTP
CEEECCCCEEEECCCCCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEC
GADGMKGAIAKAEELKAATPGSVILQQFENPANPAMHLRTTGLEIWRDTEGKVDIFVAGV
CCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEEEECCEEEEECCCCCEEEEEEEC
GTGGTISGVGEALKMRDPSVKAVAVEPADSPVLSGGKPGPHKIQGIGAGFVPKTYNASVV
CCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHH
DEIIQVQNDDAIRTSRALAEEEGLLVGISSGAAVYAATELAKRPENEGKMIVALLPDTGE
HHHHHCCCCCHHHHHHHHHHCCCEEEEECCCCEEEEHHHHHHCCCCCCCEEEEECCCCCH
RYLSTILYAFEEYPL
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]