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The map label for this gene is prtC [H]

Identifier: 29348481

GI number: 29348481

Start: 3895716

End: 3896999

Strand: Reverse

Name: prtC [H]

Synonym: BT_3072

Alternate gene names: 29348481

Gene position: 3896999-3895716 (Counterclockwise)

Preceding gene: 29348483

Following gene: 29348480

Centisome position: 62.25

GC content: 46.81

Gene sequence:

>1284_bases
ATGAATATAAAAGATTTTGAAATAATGGCGCCTGTCGGTTCGCGTGAATCCCTTGCGGCAGCCATTCAGGCTGGAGCCGA
TTCTATCTATTTTGGTATAGAAAACCTTAACATGCGTGCCCGTTCTGCCAATACGTTTACCGTTGATGACTTGCGTGAGA
TAGCTCAGACGTGTGATGAACATGGAATGAAAAGCTATCTGACCGTTAATACCATTATCTATGATAAGGATATTCCTTTG
ATGCGTACCATTGTGGACGCGGCTAAAGCGGCAGGAATATCAGCGGTGATCGCTGCCGATGTAGCGGTAATGAACTATGC
CCGTCAGATCGGTCAGGAAGTACACCTGTCTACTCAATTGAATATATCGAATGCGGAAGCCTTGAAGTTCTACGCCCAGT
TTGCCGATGTCGTAGTGCTGGCCCGTGAGTTGAATCTGGAACAGGTGGCCGAGATTTATCGTCAGATTCGGGAAGAGAAT
ATCTGCGGTCCGAGCGGAGAGCAGATTCGTATTGAAATGTTCTGTCACGGTGCCTTGTGCATGGCAGTCTCCGGTAAGTG
CTATTTGTCTCTGCACGAAATGAATCACTCGGCCAATCGGGGTGCCTGTATGCAGGTATGCCGTCGTTCTTATACGGTTC
GCGATAAGGAGACGGATGTGGAACTGGATATCGACAATGAATATATCATGTCACCGAAAGACTTGAAGACCATTCATTTT
ATGAATAAGATGATGGATGCGGGTGTGCGTGTATTCAAGATTGAGGGACGTGCCCGCGGTCCTGAGTATGTGCGTACGGT
GGTGGAATGTTATAAAGAAGCGATAAAGGCCTATCTGGATGATACGTTTACAGACGAAAAGATTGCCGCTTGGGATGAAC
GTCTGAAGGCAGTCTTTAACCGTGGCTTTTGGGATGGCTACTATCTGGGACAACGCTTGGGCGAATGGACACGGAATTAT
GGTTCGGCCGCTACGGAACGTAAAATCTATGTGGGCAAAGGTATCAAATACTTCTCCAATATCGGTGTCTCTGAGTTCTT
GGTAGAAGCTGCCGAAGTCAGTGTAGGTGACAAGCTGCTGATAACGGGACCTACCACCGGCGCAGTCTTTATGACACTGG
ACGAAGCGCGTGTCGATCTGAAGCCGGTAGAGACGGTAAAGAAAGGTCAGCGTTTCTCAATGAAATCAGAGAAGATACGT
CCCAGCGACAAATTATATAAATTGGTATCTACCGAAGAATTGAAGAAATTCAAGGGACTTGATGTAGAACAGAAAAGAGG
ATAA

Upstream 100 bases:

>100_bases
AAAACTGAAAGAACTATTTGTTGAGGTAAGTTTCACGGATACGGATTAATTCGTACCTTTGCATTCTCCTATCACATTTT
AATGATTATCAGATAGTAAA

Downstream 100 bases:

>100_bases
ACGATGAGCCAGTATATCTACGAATTGGAGATGAAGGTGCGTGATTACGAATGTGACCTTCAGGGGATTGTCAACAATGC
CAATTATCAGCATTATCTGG

Product: collagenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MNIKDFEIMAPVGSRESLAAAIQAGADSIYFGIENLNMRARSANTFTVDDLREIAQTCDEHGMKSYLTVNTIIYDKDIPL
MRTIVDAAKAAGISAVIAADVAVMNYARQIGQEVHLSTQLNISNAEALKFYAQFADVVVLARELNLEQVAEIYRQIREEN
ICGPSGEQIRIEMFCHGALCMAVSGKCYLSLHEMNHSANRGACMQVCRRSYTVRDKETDVELDIDNEYIMSPKDLKTIHF
MNKMMDAGVRVFKIEGRARGPEYVRTVVECYKEAIKAYLDDTFTDEKIAAWDERLKAVFNRGFWDGYYLGQRLGEWTRNY
GSAATERKIYVGKGIKYFSNIGVSEFLVEAAEVSVGDKLLITGPTTGAVFMTLDEARVDLKPVETVKKGQRFSMKSEKIR
PSDKLYKLVSTEELKKFKGLDVEQKRG

Sequences:

>Translated_427_residues
MNIKDFEIMAPVGSRESLAAAIQAGADSIYFGIENLNMRARSANTFTVDDLREIAQTCDEHGMKSYLTVNTIIYDKDIPL
MRTIVDAAKAAGISAVIAADVAVMNYARQIGQEVHLSTQLNISNAEALKFYAQFADVVVLARELNLEQVAEIYRQIREEN
ICGPSGEQIRIEMFCHGALCMAVSGKCYLSLHEMNHSANRGACMQVCRRSYTVRDKETDVELDIDNEYIMSPKDLKTIHF
MNKMMDAGVRVFKIEGRARGPEYVRTVVECYKEAIKAYLDDTFTDEKIAAWDERLKAVFNRGFWDGYYLGQRLGEWTRNY
GSAATERKIYVGKGIKYFSNIGVSEFLVEAAEVSVGDKLLITGPTTGAVFMTLDEARVDLKPVETVKKGQRFSMKSEKIR
PSDKLYKLVSTEELKKFKGLDVEQKRG
>Mature_427_residues
MNIKDFEIMAPVGSRESLAAAIQAGADSIYFGIENLNMRARSANTFTVDDLREIAQTCDEHGMKSYLTVNTIIYDKDIPL
MRTIVDAAKAAGISAVIAADVAVMNYARQIGQEVHLSTQLNISNAEALKFYAQFADVVVLARELNLEQVAEIYRQIREEN
ICGPSGEQIRIEMFCHGALCMAVSGKCYLSLHEMNHSANRGACMQVCRRSYTVRDKETDVELDIDNEYIMSPKDLKTIHF
MNKMMDAGVRVFKIEGRARGPEYVRTVVECYKEAIKAYLDDTFTDEKIAAWDERLKAVFNRGFWDGYYLGQRLGEWTRNY
GSAATERKIYVGKGIKYFSNIGVSEFLVEAAEVSVGDKLLITGPTTGAVFMTLDEARVDLKPVETVKKGQRFSMKSEKIR
PSDKLYKLVSTEELKKFKGLDVEQKRG

Specific function: Expresses collagenase activity. Hydrolyzes type I collagen. May play a role in virulence [H]

COG id: COG0826

COG function: function code O; Collagenase and related proteases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U32 family [H]

Homologues:

Organism=Escherichia coli, GI87081910, Length=319, Percent_Identity=31.3479623824451, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1789548, Length=326, Percent_Identity=29.7546012269939, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1788397, Length=420, Percent_Identity=26.6666666666667, Blast_Score=116, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001539 [H]

Pfam domain/function: PF01136 Peptidase_U32 [H]

EC number: 3.4.-.- [C]

Molecular weight: Translated: 48071; Mature: 48071

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS01276 PEPTIDASE_U32

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIKDFEIMAPVGSRESLAAAIQAGADSIYFGIENLNMRARSANTFTVDDLREIAQTCDE
CCCCCEEEECCCCCHHHHHHHHHCCCCEEEEEEECCCEEECCCCCEEHHHHHHHHHHHHH
HGMKSYLTVNTIIYDKDIPLMRTIVDAAKAAGISAVIAADVAVMNYARQIGQEVHLSTQL
CCCCCEEEEEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHEEEEEEE
NISNAEALKFYAQFADVVVLARELNLEQVAEIYRQIREENICGPSGEQIRIEMFCHGALC
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCEEE
MAVSGKCYLSLHEMNHSANRGACMQVCRRSYTVRDKETDVELDIDNEYIMSPKDLKTIHF
EEECCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCEECCCHHHHHHHH
MNKMMDAGVRVFKIEGRARGPEYVRTVVECYKEAIKAYLDDTFTDEKIAAWDERLKAVFN
HHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
RGFWDGYYLGQRLGEWTRNYGSAATERKIYVGKGIKYFSNIGVSEFLVEAAEVSVGDKLL
CCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHCCHHHHHHHHHHCCCCCEEE
ITGPTTGAVFMTLDEARVDLKPVETVKKGQRFSMKSEKIRPSDKLYKLVSTEELKKFKGL
EECCCCCEEEEEEECCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
DVEQKRG
CCHHCCC
>Mature Secondary Structure
MNIKDFEIMAPVGSRESLAAAIQAGADSIYFGIENLNMRARSANTFTVDDLREIAQTCDE
CCCCCEEEECCCCCHHHHHHHHHCCCCEEEEEEECCCEEECCCCCEEHHHHHHHHHHHHH
HGMKSYLTVNTIIYDKDIPLMRTIVDAAKAAGISAVIAADVAVMNYARQIGQEVHLSTQL
CCCCCEEEEEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHEEEEEEE
NISNAEALKFYAQFADVVVLARELNLEQVAEIYRQIREENICGPSGEQIRIEMFCHGALC
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCEEE
MAVSGKCYLSLHEMNHSANRGACMQVCRRSYTVRDKETDVELDIDNEYIMSPKDLKTIHF
EEECCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCEECCCHHHHHHHH
MNKMMDAGVRVFKIEGRARGPEYVRTVVECYKEAIKAYLDDTFTDEKIAAWDERLKAVFN
HHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
RGFWDGYYLGQRLGEWTRNYGSAATERKIYVGKGIKYFSNIGVSEFLVEAAEVSVGDKLL
CCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHCCHHHHHHHHHHCCCCCEEE
ITGPTTGAVFMTLDEARVDLKPVETVKKGQRFSMKSEKIRPSDKLYKLVSTEELKKFKGL
EECCCCCEEEEEEECCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
DVEQKRG
CCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12949112 [H]