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The map label for this gene is agaA [H]

Identifier: 29348474

GI number: 29348474

Start: 3886637

End: 3888148

Strand: Reverse

Name: agaA [H]

Synonym: BT_3065

Alternate gene names: 29348474

Gene position: 3888148-3886637 (Counterclockwise)

Preceding gene: 29348478

Following gene: 29348473

Centisome position: 62.11

GC content: 47.22

Gene sequence:

>1512_bases
ATGAATCAAACACGTTGTATCGTAATTTTGTCTATTCTTGCTCTTTTTGTAGCAAGCGTATCTGTTGCCGGAAATATCTC
TCTACCGGATGCTTCTCAAAAGGTTTTTGAAGGAAAACCATGTATTAACTCTCCCCGGGCGATTGGCAATTATCCTGCCA
GTCCTTTCCTCTTTTATATTCCTACTACCGGTCAGCGGCCGATGGAATGGAGTGCGGAGAAACTTCCGAAAGGATTGAAA
CTGGATTCGAAAACAGGGATTATCACAGGATCAGTAGCTTCTAAAGGTGAATATACCGTCACTCTTAAAGCTAAGAACGC
ATTGGGGACCAGTACGGAAAAGCTCGTGATCCGCATTGGTGATGACCTGTTGCTGACTCCTCCCATGGGTTGGAACAGTT
GGAATACTTTCGGGCGACATCTCACCGAAGAGTTGGTTTTGCAAACTGCGGATGCGCTGGTAGCCAACGGAATGCGGGAT
TTAGGCTATTCCTATATTAACATCGACGACTTCTGGCAGTTGCCCGAACGGGGAGCTGACGGACATTTGCAGATTAATAA
AGATAAGTTCCCGCGTGGAATAAAGTATGTGGCAGATTATCTGCACGAGCGCGGTTTCAAACTCGGAATTTATTCGGATG
CAACCGATAAGACTTGTGGCGGTGTATGCGGCAGTTACGGCTACGAAGAAGTGGATGCGAAAGATTTCGCTTCGTGGGGT
GTCGATTTGTTGAAGTATGATTATTGCAATGCTCCTGTCGACCGGGTGGAGGCTATGGAGCGCTATGCAAAGATGGGAAA
GGCGTTGCGTGGTACGGGACGTTCCATCGTGTTCTCCATTTGTGAGTGGGGACAGCGCGAACCTTGGAAATGGGCAAAAC
AGGTAGGCGGACATTTGTGGCGCGTTTCGGGAGATATAGGCGATGTCTGGAACCGGGAAGCCAACAAGTTGGGCGGGCTT
CGTGGTATCCTGAATATTCTTGAAATCAATGCTCCTTTGAGTGAATATGGCGGTCCTTCCGGTTGGAATGATCCGGATAT
GCTGGTAGTTGGTATCGGTGGTAAGAGTATGAGTATCGGATATGAATCGGAAGGTTGCACCCACGAACAGTATAAGTCTC
ATTTTGCATTATGGTGTATGATGGCTTCTCCATTACTTTGCGGAAATGACGTGCGAAGTATGAATGACAGTACATTGCAG
GTTCTGTTGGATAGGGACCTGATTGCTATCAACCAGGATGTGTTGGGCAAGCAGGCGGAGCGCAGCATCCGTGCAGATCA
TTATGATATTTGGGTAAAGCCGTTGGCTGACGGTCGCAAAGCGGTAGCTTGTTTTAACCGGGCGGATACTCCACGTACTA
TAGAATTAAATTCAAAAACGGTGGAAGACCTGTCTTTGGAGCAGGTGTATAGCCTTGACAGCCGTTCTATGGAAAATGCT
GCGAATAACATAATGGTGGACTTGGCGCCTTACCAATGCAAGGTTTATATCTGTGGAAAGCCGAAAAAATAG

Upstream 100 bases:

>100_bases
TTTTTAGTTTGCGGGCGGCAAAGGTATAAAAAAAATGTTTATCACCATTCATCCTGCTTCTTTTCCCTTATCTTAGTGGC
GTAATAAAAAACAAACAACT

Downstream 100 bases:

>100_bases
TCAATGACAGTACTGGAATGACAGTAATAAATCTCTGAGGTAAAATAAAGAAAGGTTGGGGCATAAAAAAAGGAGCAACG
CCTTGCTCCAACCTTTGTTA

Product: alpha-galactosidase

Products: NA

Alternate protein names: Alpha-D-galactoside galactohydrolase; Alpha-galactosidase 27A; Melibiase [H]

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MNQTRCIVILSILALFVASVSVAGNISLPDASQKVFEGKPCINSPRAIGNYPASPFLFYIPTTGQRPMEWSAEKLPKGLK
LDSKTGIITGSVASKGEYTVTLKAKNALGTSTEKLVIRIGDDLLLTPPMGWNSWNTFGRHLTEELVLQTADALVANGMRD
LGYSYINIDDFWQLPERGADGHLQINKDKFPRGIKYVADYLHERGFKLGIYSDATDKTCGGVCGSYGYEEVDAKDFASWG
VDLLKYDYCNAPVDRVEAMERYAKMGKALRGTGRSIVFSICEWGQREPWKWAKQVGGHLWRVSGDIGDVWNREANKLGGL
RGILNILEINAPLSEYGGPSGWNDPDMLVVGIGGKSMSIGYESEGCTHEQYKSHFALWCMMASPLLCGNDVRSMNDSTLQ
VLLDRDLIAINQDVLGKQAERSIRADHYDIWVKPLADGRKAVACFNRADTPRTIELNSKTVEDLSLEQVYSLDSRSMENA
ANNIMVDLAPYQCKVYICGKPKK

Sequences:

>Translated_503_residues
MNQTRCIVILSILALFVASVSVAGNISLPDASQKVFEGKPCINSPRAIGNYPASPFLFYIPTTGQRPMEWSAEKLPKGLK
LDSKTGIITGSVASKGEYTVTLKAKNALGTSTEKLVIRIGDDLLLTPPMGWNSWNTFGRHLTEELVLQTADALVANGMRD
LGYSYINIDDFWQLPERGADGHLQINKDKFPRGIKYVADYLHERGFKLGIYSDATDKTCGGVCGSYGYEEVDAKDFASWG
VDLLKYDYCNAPVDRVEAMERYAKMGKALRGTGRSIVFSICEWGQREPWKWAKQVGGHLWRVSGDIGDVWNREANKLGGL
RGILNILEINAPLSEYGGPSGWNDPDMLVVGIGGKSMSIGYESEGCTHEQYKSHFALWCMMASPLLCGNDVRSMNDSTLQ
VLLDRDLIAINQDVLGKQAERSIRADHYDIWVKPLADGRKAVACFNRADTPRTIELNSKTVEDLSLEQVYSLDSRSMENA
ANNIMVDLAPYQCKVYICGKPKK
>Mature_503_residues
MNQTRCIVILSILALFVASVSVAGNISLPDASQKVFEGKPCINSPRAIGNYPASPFLFYIPTTGQRPMEWSAEKLPKGLK
LDSKTGIITGSVASKGEYTVTLKAKNALGTSTEKLVIRIGDDLLLTPPMGWNSWNTFGRHLTEELVLQTADALVANGMRD
LGYSYINIDDFWQLPERGADGHLQINKDKFPRGIKYVADYLHERGFKLGIYSDATDKTCGGVCGSYGYEEVDAKDFASWG
VDLLKYDYCNAPVDRVEAMERYAKMGKALRGTGRSIVFSICEWGQREPWKWAKQVGGHLWRVSGDIGDVWNREANKLGGL
RGILNILEINAPLSEYGGPSGWNDPDMLVVGIGGKSMSIGYESEGCTHEQYKSHFALWCMMASPLLCGNDVRSMNDSTLQ
VLLDRDLIAINQDVLGKQAERSIRADHYDIWVKPLADGRKAVACFNRADTPRTIELNSKTVEDLSLEQVYSLDSRSMENA
ANNIMVDLAPYQCKVYICGKPKK

Specific function: Hydrolyzes galactomannan found in plant cell wall, by cleaving alpha-1,6-D-galactose side-chains from the mannan backbone. Appears to act in synergy with mannanase (ManA) to elicit hydrolysis of galactomannan. Has greater activity against galactomannans w

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 27 family [H]

Homologues:

Organism=Homo sapiens, GI4504009, Length=355, Percent_Identity=38.8732394366197, Blast_Score=238, Evalue=9e-63,
Organism=Homo sapiens, GI4557781, Length=417, Percent_Identity=34.7721822541966, Blast_Score=199, Evalue=7e-51,
Organism=Caenorhabditis elegans, GI17562946, Length=390, Percent_Identity=32.8205128205128, Blast_Score=178, Evalue=7e-45,
Organism=Drosophila melanogaster, GI24583253, Length=368, Percent_Identity=36.6847826086957, Blast_Score=211, Evalue=1e-54,
Organism=Drosophila melanogaster, GI19922382, Length=400, Percent_Identity=31.25, Blast_Score=182, Evalue=7e-46,
Organism=Drosophila melanogaster, GI24654142, Length=400, Percent_Identity=31.25, Blast_Score=182, Evalue=7e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013780
- InterPro:   IPR002241
- InterPro:   IPR017853
- InterPro:   IPR000111 [H]

Pfam domain/function: PF02065 Melibiase [H]

EC number: =3.2.1.22 [H]

Molecular weight: Translated: 55699; Mature: 55699

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQTRCIVILSILALFVASVSVAGNISLPDASQKVFEGKPCINSPRAIGNYPASPFLFYI
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCEEEEE
PTTGQRPMEWSAEKLPKGLKLDSKTGIITGSVASKGEYTVTLKAKNALGTSTEKLVIRIG
CCCCCCCCCCCHHHCCCCCEECCCCCEEECCCCCCCCEEEEEEECCCCCCCCCEEEEEEC
DDLLLTPPMGWNSWNTFGRHLTEELVLQTADALVANGMRDLGYSYINIDDFWQLPERGAD
CCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCEEECHHHHHCCCCCCCC
GHLQINKDKFPRGIKYVADYLHERGFKLGIYSDATDKTCGGVCGSYGYEEVDAKDFASWG
CCEEECCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHCCCCCCCCHHHHHHHC
VDLLKYDYCNAPVDRVEAMERYAKMGKALRGTGRSIVFSICEWGQREPWKWAKQVGGHLW
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHCCEEE
RVSGDIGDVWNREANKLGGLRGILNILEINAPLSEYGGPSGWNDPDMLVVGIGGKSMSIG
EEECCHHHHHCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCEEEEEECCCEEECC
YESEGCTHEQYKSHFALWCMMASPLLCGNDVRSMNDSTLQVLLDRDLIAINQDVLGKQAE
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCEEEEECCCCEEECHHHHHHHHH
RSIRADHYDIWVKPLADGRKAVACFNRADTPRTIELNSKTVEDLSLEQVYSLDSRSMENA
HHHCCCCEEEEEEECCCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHCCHHHHHHH
ANNIMVDLAPYQCKVYICGKPKK
CCCEEEEECCCEEEEEEECCCCC
>Mature Secondary Structure
MNQTRCIVILSILALFVASVSVAGNISLPDASQKVFEGKPCINSPRAIGNYPASPFLFYI
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCEEEEE
PTTGQRPMEWSAEKLPKGLKLDSKTGIITGSVASKGEYTVTLKAKNALGTSTEKLVIRIG
CCCCCCCCCCCHHHCCCCCEECCCCCEEECCCCCCCCEEEEEEECCCCCCCCCEEEEEEC
DDLLLTPPMGWNSWNTFGRHLTEELVLQTADALVANGMRDLGYSYINIDDFWQLPERGAD
CCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCEEECHHHHHCCCCCCCC
GHLQINKDKFPRGIKYVADYLHERGFKLGIYSDATDKTCGGVCGSYGYEEVDAKDFASWG
CCEEECCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHCCCCCCCCHHHHHHHC
VDLLKYDYCNAPVDRVEAMERYAKMGKALRGTGRSIVFSICEWGQREPWKWAKQVGGHLW
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHCCEEE
RVSGDIGDVWNREANKLGGLRGILNILEINAPLSEYGGPSGWNDPDMLVVGIGGKSMSIG
EEECCHHHHHCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCEEEEEECCCEEECC
YESEGCTHEQYKSHFALWCMMASPLLCGNDVRSMNDSTLQVLLDRDLIAINQDVLGKQAE
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCEEEEECCCCEEECHHHHHHHHH
RSIRADHYDIWVKPLADGRKAVACFNRADTPRTIELNSKTVEDLSLEQVYSLDSRSMENA
HHHCCCCEEEEEEECCCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHCCHHHHHHH
ANNIMVDLAPYQCKVYICGKPKK
CCCEEEEECCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA