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The map label for this gene is cobB

Identifier: 29348384

GI number: 29348384

Start: 3753745

End: 3754449

Strand: Reverse

Name: cobB

Synonym: BT_2975

Alternate gene names: 29348384

Gene position: 3754449-3753745 (Counterclockwise)

Preceding gene: 29348409

Following gene: 29348380

Centisome position: 59.97

GC content: 48.37

Gene sequence:

>705_bases
ATGAAGAATCTGGTAGTATTGACAGGTGCGGGCATGAGTGCCGAAAGTGGAATCAGCACATTTCGTGATGCGGGCGGTTT
GTGGGATAAATATCCTGTGGAGCAGGTAGCTACTCCCGAAGGATATCAGCGTGATCCTGCATTGGTGATTAACTTCTATA
ATGCACGAAGGAAACAATTGCTGGAAGTGAAGCCGAATCGCGGACATGAACTGCTGGCGGAGCTGGAAAAGAATTTCAAT
GTGACGGTTATCACACAGAATGTAGATAATCTGCACGAACGGGCAGGAAGCAGCCATATCGTTCACTTGCATGGTGAGTT
GACAAAGGTCTGTTCCAGCAGAGACCCGTATAACCCTCATTATATAAAGGAACTGAAGCCGGAAGAGTATGAAGTGAAAA
TGGGGGATAAGGCTGGTGACGGAACTCAGTTGCGTCCTTTTATTGTCTGGTTCGGAGAGGCGGTGCCGGAGATTGAGACA
GCTGTCCGATATGTAGAAAAGGCGGATATATTCGTTATCATCGGCACATCGCTCAATGTATATCCTGCGGCAGGTTTGCT
GCATTATGTACCGAGAGGAGCAGAGGTGTATCTGATAGACCCGAAGCCGGTGGATACACATACTTCCCGTTCGATACACG
TCCTTCGGAAAGGAGCTTCTGAAGGGGTGGAGGAACTGAAACAACTGCTTATTCCAGCTCCGTAG

Upstream 100 bases:

>100_bases
TTCTTGTATACCTTGGATTAGATTATGCGGGCGGAAGTCTTCTTTTATTCAATGATTCTGATTACATTTGTACATCTTTT
AATAATAAGGAGGAGCGGTT

Downstream 100 bases:

>100_bases
CAAAGATATGACTATTCCGGAGTGTTTCCGAAATCTCGAGTGCGGTGATATTGCTGCACTCGATTCTTAATACCTTCTCC
CAGTCCTCGAAATCTACATT

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MKNLVVLTGAGMSAESGISTFRDAGGLWDKYPVEQVATPEGYQRDPALVINFYNARRKQLLEVKPNRGHELLAELEKNFN
VTVITQNVDNLHERAGSSHIVHLHGELTKVCSSRDPYNPHYIKELKPEEYEVKMGDKAGDGTQLRPFIVWFGEAVPEIET
AVRYVEKADIFVIIGTSLNVYPAAGLLHYVPRGAEVYLIDPKPVDTHTSRSIHVLRKGASEGVEELKQLLIPAP

Sequences:

>Translated_234_residues
MKNLVVLTGAGMSAESGISTFRDAGGLWDKYPVEQVATPEGYQRDPALVINFYNARRKQLLEVKPNRGHELLAELEKNFN
VTVITQNVDNLHERAGSSHIVHLHGELTKVCSSRDPYNPHYIKELKPEEYEVKMGDKAGDGTQLRPFIVWFGEAVPEIET
AVRYVEKADIFVIIGTSLNVYPAAGLLHYVPRGAEVYLIDPKPVDTHTSRSIHVLRKGASEGVEELKQLLIPAP
>Mature_234_residues
MKNLVVLTGAGMSAESGISTFRDAGGLWDKYPVEQVATPEGYQRDPALVINFYNARRKQLLEVKPNRGHELLAELEKNFN
VTVITQNVDNLHERAGSSHIVHLHGELTKVCSSRDPYNPHYIKELKPEEYEVKMGDKAGDGTQLRPFIVWFGEAVPEIET
AVRYVEKADIFVIIGTSLNVYPAAGLLHYVPRGAEVYLIDPKPVDTHTSRSIHVLRKGASEGVEELKQLLIPAP

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain

Homologues:

Organism=Homo sapiens, GI300795542, Length=191, Percent_Identity=40.8376963350785, Blast_Score=140, Evalue=9e-34,
Organism=Homo sapiens, GI6912664, Length=208, Percent_Identity=37.9807692307692, Blast_Score=135, Evalue=2e-32,
Organism=Homo sapiens, GI13787215, Length=208, Percent_Identity=37.9807692307692, Blast_Score=135, Evalue=3e-32,
Organism=Escherichia coli, GI308199517, Length=189, Percent_Identity=48.6772486772487, Blast_Score=171, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17541892, Length=213, Percent_Identity=30.5164319248826, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NPD_BACTN (Q8A3H9)

Other databases:

- EMBL:   AE015928
- RefSeq:   NP_811887.1
- ProteinModelPortal:   Q8A3H9
- SMR:   Q8A3H9
- GeneID:   1076000
- GenomeReviews:   AE015928_GR
- KEGG:   bth:BT_2975
- NMPDR:   fig|226186.1.peg.2974
- HOGENOM:   HBG641281
- OMA:   DADGLWE
- PhylomeDB:   Q8A3H9
- ProtClustDB:   PRK00481
- BioCyc:   BTHE226186:BT_2975-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01121
- InterPro:   IPR003000
- PANTHER:   PTHR11085

Pfam domain/function: PF02146 SIR2

EC number: 3.5.1.- [C]

Molecular weight: Translated: 26080; Mature: 26080

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS50305 SIRTUIN

Important sites: ACT_SITE 104-104

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLVVLTGAGMSAESGISTFRDAGGLWDKYPVEQVATPEGYQRDPALVINFYNARRKQL
CCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCEEEEEECCHHHHHE
LEVKPNRGHELLAELEKNFNVTVITQNVDNLHERAGSSHIVHLHGELTKVCSSRDPYNPH
EEECCCCCHHHHHHHHHCCCEEEEECCHHHHHHHCCCCCEEEECCHHHHHHCCCCCCCCC
YIKELKPEEYEVKMGDKAGDGTQLRPFIVWFGEAVPEIETAVRYVEKADIFVIIGTSLNV
HHHHCCCCCEEEEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCEEEEECCCCCC
YPAAGLLHYVPRGAEVYLIDPKPVDTHTSRSIHVLRKGASEGVEELKQLLIPAP
CCHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKNLVVLTGAGMSAESGISTFRDAGGLWDKYPVEQVATPEGYQRDPALVINFYNARRKQL
CCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCEEEEEECCHHHHHE
LEVKPNRGHELLAELEKNFNVTVITQNVDNLHERAGSSHIVHLHGELTKVCSSRDPYNPH
EEECCCCCHHHHHHHHHCCCEEEEECCHHHHHHHCCCCCEEEECCHHHHHHCCCCCCCCC
YIKELKPEEYEVKMGDKAGDGTQLRPFIVWFGEAVPEIETAVRYVEKADIFVIIGTSLNV
HHHHCCCCCEEEEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCEEEEECCCCCC
YPAAGLLHYVPRGAEVYLIDPKPVDTHTSRSIHVLRKGASEGVEELKQLLIPAP
CCHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928