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The map label for this gene is gne [H]

Identifier: 29348296

GI number: 29348296

Start: 3606128

End: 3607108

Strand: Reverse

Name: gne [H]

Synonym: BT_2887

Alternate gene names: 29348296

Gene position: 3607108-3606128 (Counterclockwise)

Preceding gene: 29348297

Following gene: 29348295

Centisome position: 57.62

GC content: 43.83

Gene sequence:

>981_bases
ATGAAAATAGCTATTATCGGTGGTTCCGGATTCGTGGGAAGTCGTTTGATCGGGCTGTTACAGACTGTTCCGAACATTGA
ACTGCTCAATATTGACAAGCAGCAGAGTGAACTTTATCCTCATCTGACACAAATAGCTGATGTGCAGGATGTACAGAAGC
TGACGGAGTTGCTGGCAGGAACTGATCTTGTGGTTTTATTGGCTGCCGAACATAAAGACAACGTAACTCCTGCATCGCTT
TACTATACGGTGAATGTGGAAGGGACACGTAATACGCTTCAGGCTATGGAAAGTAATGGAGTTGCCCGTTTGGTATTCAC
CAGTTCTGTTGCTGTCTATGGATTGAACAAAGATAATCCATCAGAATTGCATCCGGCCGATCCGTTCAATGATTACGGTC
GTAGTAAGTGGCAGGCGGAATGTATGCTGCAGGAGTGGTATGATACGCATCGGGAGTGGAACATTCATATTCTTCGTCCC
ACAGTTATCTTTGGAGAGGGCAACAGGGGGAATGTATATAATTTGTTGCGGCAGATTACTTCCGGGCGATTTCTGATGGT
AGGAGACGGTGAAAACCGGAAGTCGATGGCCTATGTCGGTAACGTAGTTGCTTTTATCGCTTTTCTGATTGAGAATAATA
TGGAGGGATATCATGTATTCAATTATATCGATAAGCCGGATTTCACAATGAATGATTTGGTGTATCATGTCGGGGAGGTG
TTGAATAAACATATTCCTACTACACATTACCCCTATTGGTTAGGAATGTTGGGTGGATATTGCTTTGATGCTTTGGCTAA
GATGACGGGAAGAAAATTGTCGGTGAGTTCTGTTCGTGTGAAGAAGTTCTGTGCGGTGACTCAGTTTGATTCTGTTAAAG
TACAGTCATCGGGTTTTAAGCCGGCTTTCTCAATGGAAGAAGGGTTGAGACGGACGTTGCAATACGAGTTTGGTTCAGGA
GCTGAAGAAGCTATTAATTGA

Upstream 100 bases:

>100_bases
TTTATAGATATCGTACTGTGGATTGCTTTCCATTTATGGTTGGACAGGCGAGAGCTTATCCGGGCAAAACATGGGGTGGC
AAATAATTAAATTTAATGAT

Downstream 100 bases:

>100_bases
TAAACTAGGCTTTTTCCTTTTTACAGGATAGAAAAGAAGGAAAAAAGTTAGATAGATAAGACATAAAATTTTTCAGGTAG
CCGTCATGTATGTGACTGGC

Product: putative UDP-galactose 4-epimerase

Products: NA

Alternate protein names: UDP-GlcNAc 4-epimerase [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MKIAIIGGSGFVGSRLIGLLQTVPNIELLNIDKQQSELYPHLTQIADVQDVQKLTELLAGTDLVVLLAAEHKDNVTPASL
YYTVNVEGTRNTLQAMESNGVARLVFTSSVAVYGLNKDNPSELHPADPFNDYGRSKWQAECMLQEWYDTHREWNIHILRP
TVIFGEGNRGNVYNLLRQITSGRFLMVGDGENRKSMAYVGNVVAFIAFLIENNMEGYHVFNYIDKPDFTMNDLVYHVGEV
LNKHIPTTHYPYWLGMLGGYCFDALAKMTGRKLSVSSVRVKKFCAVTQFDSVKVQSSGFKPAFSMEEGLRRTLQYEFGSG
AEEAIN

Sequences:

>Translated_326_residues
MKIAIIGGSGFVGSRLIGLLQTVPNIELLNIDKQQSELYPHLTQIADVQDVQKLTELLAGTDLVVLLAAEHKDNVTPASL
YYTVNVEGTRNTLQAMESNGVARLVFTSSVAVYGLNKDNPSELHPADPFNDYGRSKWQAECMLQEWYDTHREWNIHILRP
TVIFGEGNRGNVYNLLRQITSGRFLMVGDGENRKSMAYVGNVVAFIAFLIENNMEGYHVFNYIDKPDFTMNDLVYHVGEV
LNKHIPTTHYPYWLGMLGGYCFDALAKMTGRKLSVSSVRVKKFCAVTQFDSVKVQSSGFKPAFSMEEGLRRTLQYEFGSG
AEEAIN
>Mature_326_residues
MKIAIIGGSGFVGSRLIGLLQTVPNIELLNIDKQQSELYPHLTQIADVQDVQKLTELLAGTDLVVLLAAEHKDNVTPASL
YYTVNVEGTRNTLQAMESNGVARLVFTSSVAVYGLNKDNPSELHPADPFNDYGRSKWQAECMLQEWYDTHREWNIHILRP
TVIFGEGNRGNVYNLLRQITSGRFLMVGDGENRKSMAYVGNVVAFIAFLIENNMEGYHVFNYIDKPDFTMNDLVYHVGEV
LNKHIPTTHYPYWLGMLGGYCFDALAKMTGRKLSVSSVRVKKFCAVTQFDSVKVQSSGFKPAFSMEEGLRRTLQYEFGSG
AEEAIN

Specific function: Converts UDP-GlcNAc to UDP-GalNAc. Essential for O antigen synthesis [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI193211614, Length=339, Percent_Identity=23.3038348082596, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI8393516, Length=339, Percent_Identity=23.3038348082596, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1786974, Length=229, Percent_Identity=27.0742358078603, Blast_Score=65, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6321437, Length=342, Percent_Identity=27.4853801169591, Blast_Score=95, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.7 [H]

Molecular weight: Translated: 36565; Mature: 36565

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAIIGGSGFVGSRLIGLLQTVPNIELLNIDKQQSELYPHLTQIADVQDVQKLTELLAG
CEEEEECCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TDLVVLLAAEHKDNVTPASLYYTVNVEGTRNTLQAMESNGVARLVFTSSVAVYGLNKDNP
CCEEEEEEECCCCCCCCEEEEEEEEECCCHHHHHHHHCCCEEEEEEECCEEEEECCCCCC
SELHPADPFNDYGRSKWQAECMLQEWYDTHREWNIHILRPTVIFGEGNRGNVYNLLRQIT
CCCCCCCCCCHHCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHH
SGRFLMVGDGENRKSMAYVGNVVAFIAFLIENNMEGYHVFNYIDKPDFTMNDLVYHVGEV
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHH
LNKHIPTTHYPYWLGMLGGYCFDALAKMTGRKLSVSSVRVKKFCAVTQFDSVKVQSSGFK
HHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCCCCEEEECCCCC
PAFSMEEGLRRTLQYEFGSGAEEAIN
CCCCHHHHHHHHHHHCCCCCHHHHCC
>Mature Secondary Structure
MKIAIIGGSGFVGSRLIGLLQTVPNIELLNIDKQQSELYPHLTQIADVQDVQKLTELLAG
CEEEEECCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TDLVVLLAAEHKDNVTPASLYYTVNVEGTRNTLQAMESNGVARLVFTSSVAVYGLNKDNP
CCEEEEEEECCCCCCCCEEEEEEEEECCCHHHHHHHHCCCEEEEEEECCEEEEECCCCCC
SELHPADPFNDYGRSKWQAECMLQEWYDTHREWNIHILRPTVIFGEGNRGNVYNLLRQIT
CCCCCCCCCCHHCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHH
SGRFLMVGDGENRKSMAYVGNVVAFIAFLIENNMEGYHVFNYIDKPDFTMNDLVYHVGEV
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHH
LNKHIPTTHYPYWLGMLGGYCFDALAKMTGRKLSVSSVRVKKFCAVTQFDSVKVQSSGFK
HHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCCCCEEEECCCCC
PAFSMEEGLRRTLQYEFGSGAEEAIN
CCCCHHHHHHHHHHHCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11976290; 11206551; 11258796 [H]