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The map label for this gene is amsD [H]

Identifier: 29348275

GI number: 29348275

Start: 3586412

End: 3587551

Strand: Reverse

Name: amsD [H]

Synonym: BT_2866

Alternate gene names: 29348275

Gene position: 3587551-3586412 (Counterclockwise)

Preceding gene: 29348276

Following gene: 29348274

Centisome position: 57.31

GC content: 40.53

Gene sequence:

>1140_bases
ATGAAAATCGTTTACTGTATAGCCGGAACCTACAATTCCGGAGGAATGGAACGGGTATTGGCAAATAAAGCCAATTATCT
GGTCAGTCATGGATATGAAGTTGTTATTATTACTACAGATCAACGTGAAAAGCAATCCTTTTTCGAACTGGACCAACGTA
TCAACTGCTGTGACTTGAGAATCAACTATGAAGAAAATAACGGGAAGTCATTTCTTAATAAGTTAGCCAATTATCCTTTC
AAACAGTGGAAACATCGGAAGAGGCTGACGGCTTGTCTGAAACAACTTAAGGCTGACATTGTGGTTTCTATGTTCTGCAA
TGATGCTTCTTTTCTATGGAAGATTGATGATGGCAGTAAGAAAGTACTTGAAATTCATTTTTCCCGTTATAAGCGTTTGC
AATATGGACGTAAGGGAGTCTGGAAAATTGCAGACCGATGGAGAAGCCGGATGGATGAAAGAACCGTGAGGAAATACGAT
CGTTTTGTAGTACTGACTGCCGAGGATAAGGCATATTGGGGAAATCTGTCCAATATGATAGTGATCCCCAATGCATTGAG
TCATTCTACAACAGAACTTCATCTTTCTCCCCTTACAGCCCGAAAAGTGATTGCAATAGGGCGATATGGGTATCAGAAAG
GGTTTGACTATTTGATAGAAGCATGGGAAATTATTCATTGTGCCCAACCTGCTTGGACACTGGATATAATTGGTGATGGA
GAATGGACGGACCGTCTGCAACGACAAATTAAGAGAAAAAGGCTGAATCATTGTGTCTTTTTGAAACCACCGACCGGACA
GATAGAAGAAGAGTACCGACAAGCCTCTTTGCTGGTACTGTCTTCTCGTTATGAGGGGTTGCCTATGGTATTATTAGAAG
CACAGTCGTTCGGATTACCCATTGTTTCTTTTGCCTGTAAATGTGGCCCTGGAGATGTAATAACTGATGGTAAAAATGGT
TTTTTGGTATCGGTAGGAAATCTTCCGATGTTAGCCGACAGGATAATGAGACTGATGGAAGATGAAGGTTTGCGTAAACG
TATGGGAATGAATGCGTACCATAATTCAAAAACATTCTCGGAGGAACGAATTATGCAATGTTGGATTGATATGTTCGATA
AGTTAGTATCACAAAGATGA

Upstream 100 bases:

>100_bases
TGAGTTTCATTATCTGGATGAAAGTAGCTACTGATCTATTTTTTATTTATGCTTTATTTTATTGTCTGGACTGGAAAATG
GAACCAGTTCTTATAGAAGA

Downstream 100 bases:

>100_bases
GTAGCAAGCGAAAGACGATTGTTGTCTCAGCGGTTAGTTTACGTAAGGGAGGTACATTAACGATATTGCAAGATTGCTTA
TGTTATCTTTCTGCTTTGGC

Product: putative glycosyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MKIVYCIAGTYNSGGMERVLANKANYLVSHGYEVVIITTDQREKQSFFELDQRINCCDLRINYEENNGKSFLNKLANYPF
KQWKHRKRLTACLKQLKADIVVSMFCNDASFLWKIDDGSKKVLEIHFSRYKRLQYGRKGVWKIADRWRSRMDERTVRKYD
RFVVLTAEDKAYWGNLSNMIVIPNALSHSTTELHLSPLTARKVIAIGRYGYQKGFDYLIEAWEIIHCAQPAWTLDIIGDG
EWTDRLQRQIKRKRLNHCVFLKPPTGQIEEEYRQASLLVLSSRYEGLPMVLLEAQSFGLPIVSFACKCGPGDVITDGKNG
FLVSVGNLPMLADRIMRLMEDEGLRKRMGMNAYHNSKTFSEERIMQCWIDMFDKLVSQR

Sequences:

>Translated_379_residues
MKIVYCIAGTYNSGGMERVLANKANYLVSHGYEVVIITTDQREKQSFFELDQRINCCDLRINYEENNGKSFLNKLANYPF
KQWKHRKRLTACLKQLKADIVVSMFCNDASFLWKIDDGSKKVLEIHFSRYKRLQYGRKGVWKIADRWRSRMDERTVRKYD
RFVVLTAEDKAYWGNLSNMIVIPNALSHSTTELHLSPLTARKVIAIGRYGYQKGFDYLIEAWEIIHCAQPAWTLDIIGDG
EWTDRLQRQIKRKRLNHCVFLKPPTGQIEEEYRQASLLVLSSRYEGLPMVLLEAQSFGLPIVSFACKCGPGDVITDGKNG
FLVSVGNLPMLADRIMRLMEDEGLRKRMGMNAYHNSKTFSEERIMQCWIDMFDKLVSQR
>Mature_379_residues
MKIVYCIAGTYNSGGMERVLANKANYLVSHGYEVVIITTDQREKQSFFELDQRINCCDLRINYEENNGKSFLNKLANYPF
KQWKHRKRLTACLKQLKADIVVSMFCNDASFLWKIDDGSKKVLEIHFSRYKRLQYGRKGVWKIADRWRSRMDERTVRKYD
RFVVLTAEDKAYWGNLSNMIVIPNALSHSTTELHLSPLTARKVIAIGRYGYQKGFDYLIEAWEIIHCAQPAWTLDIIGDG
EWTDRLQRQIKRKRLNHCVFLKPPTGQIEEEYRQASLLVLSSRYEGLPMVLLEAQSFGLPIVSFACKCGPGDVITDGKNG
FLVSVGNLPMLADRIMRLMEDEGLRKRMGMNAYHNSKTFSEERIMQCWIDMFDKLVSQR

Specific function: Involved in the biosynthesis of amylovoran which functions as a virulence factor. May be involved in the formation of galactose alpha-1,6 linkages in amylovoran [H]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI226510984, Length=105, Percent_Identity=31.4285714285714, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: 2.4.1.- [C]

Molecular weight: Translated: 44060; Mature: 44060

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVYCIAGTYNSGGMERVLANKANYLVSHGYEVVIITTDQREKQSFFELDQRINCCDLR
CEEEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHCCCCEEEEE
INYEENNGKSFLNKLANYPFKQWKHRKRLTACLKQLKADIVVSMFCNDASFLWKIDDGSK
EEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEHEEECCCEEEEEECCCCC
KVLEIHFSRYKRLQYGRKGVWKIADRWRSRMDERTVRKYDRFVVLTAEDKAYWGNLSNMI
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHCCCCCEE
VIPNALSHSTTELHLSPLTARKVIAIGRYGYQKGFDYLIEAWEIIHCAQPAWTLDIIGDG
EECCCCCCCCCEEEECCCCHHHHHEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
EWTDRLQRQIKRKRLNHCVFLKPPTGQIEEEYRQASLLVLSSRYEGLPMVLLEAQSFGLP
HHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHEEEECCCCCCCEEEEECCCCCCH
IVSFACKCGPGDVITDGKNGFLVSVGNLPMLADRIMRLMEDEGLRKRMGMNAYHNSKTFS
HHHHHCCCCCCCEEECCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCC
EERIMQCWIDMFDKLVSQR
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIVYCIAGTYNSGGMERVLANKANYLVSHGYEVVIITTDQREKQSFFELDQRINCCDLR
CEEEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHCCCCEEEEE
INYEENNGKSFLNKLANYPFKQWKHRKRLTACLKQLKADIVVSMFCNDASFLWKIDDGSK
EEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEHEEECCCEEEEEECCCCC
KVLEIHFSRYKRLQYGRKGVWKIADRWRSRMDERTVRKYDRFVVLTAEDKAYWGNLSNMI
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHCCCCCEE
VIPNALSHSTTELHLSPLTARKVIAIGRYGYQKGFDYLIEAWEIIHCAQPAWTLDIIGDG
EECCCCCCCCCEEEECCCCHHHHHEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
EWTDRLQRQIKRKRLNHCVFLKPPTGQIEEEYRQASLLVLSSRYEGLPMVLLEAQSFGLP
HHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHEEEECCCCCCCEEEEECCCCCCH
IVSFACKCGPGDVITDGKNGFLVSVGNLPMLADRIMRLMEDEGLRKRMGMNAYHNSKTFS
HHHHHCCCCCCCEEECCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCC
EERIMQCWIDMFDKLVSQR
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Glycosyltransferases; Hexosyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7596293 [H]