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The map label for this gene is hupB [H]

Identifier: 29348239

GI number: 29348239

Start: 3528957

End: 3529229

Strand: Reverse

Name: hupB [H]

Synonym: BT_2830

Alternate gene names: 29348239

Gene position: 3529229-3528957 (Counterclockwise)

Preceding gene: 29348247

Following gene: 29348238

Centisome position: 56.37

GC content: 39.19

Gene sequence:

>273_bases
ATGAATAAGTCTGAACTTATTAGTGCAATGGCTGCTGAAGCTCAAATGAGTAAAGCCGATGCGAAGAAAGCTCTTGATGC
TTTCATTTCTTCAGTTACTAACGCTATGAAAGCTGGTGATAAGGTAGCTTTGGTTGGTTTTGGAACGTTCTCTGTAAGTG
AAAGAGCAGCAAGAACCGGTATTAATCCTTCTACAAAGGCAGCTATTACTATTCCTGCTAAGAAAGTAGCTAAATTTAAA
GCTGGTGCTGAATTGTCTGCAGCTGTTGAATAA

Upstream 100 bases:

>100_bases
ATGTTTTTATTTGATATGTTGTTTTGTTCATCTATATTTGTAAAGTCGTAATGGGAAATCTTTTGTGTAACATATATATT
AATTAATCATTTTACGTATC

Downstream 100 bases:

>100_bases
ACATATAGTTGAAAGAAATTAGAAGAGGGAGGCGCGTGCGTCTCCTTTTTTTGTATCTTTGCGCGCAAGAAACAACTTGA
TTATGAAGATAGAAGATAAA

Product: DNA-binding protein HU-beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 90; Mature: 90

Protein sequence:

>90_residues
MNKSELISAMAAEAQMSKADAKKALDAFISSVTNAMKAGDKVALVGFGTFSVSERAARTGINPSTKAAITIPAKKVAKFK
AGAELSAAVE

Sequences:

>Translated_90_residues
MNKSELISAMAAEAQMSKADAKKALDAFISSVTNAMKAGDKVALVGFGTFSVSERAARTGINPSTKAAITIPAKKVAKFK
AGAELSAAVE
>Mature_90_residues
MNKSELISAMAAEAQMSKADAKKALDAFISSVTNAMKAGDKVALVGFGTFSVSERAARTGINPSTKAAITIPAKKVAKFK
AGAELSAAVE

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions [H]

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family [H]

Homologues:

Organism=Escherichia coli, GI1786644, Length=89, Percent_Identity=62.9213483146067, Blast_Score=102, Evalue=5e-24,
Organism=Escherichia coli, GI1790433, Length=89, Percent_Identity=48.314606741573, Blast_Score=83, Evalue=4e-18,
Organism=Escherichia coli, GI1788005, Length=90, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=3e-13,

Paralogues:

None

Copy number: 860 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). 2040 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000119
- InterPro:   IPR020816
- InterPro:   IPR010992 [H]

Pfam domain/function: PF00216 Bac_DNA_binding [H]

EC number: NA

Molecular weight: Translated: 9190; Mature: 9190

Theoretical pI: Translated: 10.56; Mature: 10.56

Prosite motif: PS00045 HISTONE_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKSELISAMAAEAQMSKADAKKALDAFISSVTNAMKAGDKVALVGFGTFSVSERAARTG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCC
INPSTKAAITIPAKKVAKFKAGAELSAAVE
CCCCCCEEEECCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNKSELISAMAAEAQMSKADAKKALDAFISSVTNAMKAGDKVALVGFGTFSVSERAARTG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCC
INPSTKAAITIPAKKVAKFKAGAELSAAVE
CCCCCCEEEECCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7867950; 10984043; 6793399 [H]