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The map label for this gene is ahpF [H]

Identifier: 29348220

GI number: 29348220

Start: 3491839

End: 3493410

Strand: Reverse

Name: ahpF [H]

Synonym: BT_2811

Alternate gene names: 29348220

Gene position: 3493410-3491839 (Counterclockwise)

Preceding gene: 29348221

Following gene: 29348219

Centisome position: 55.8

GC content: 47.07

Gene sequence:

>1572_bases
ATGAAAGGAGAAAGAGAGATGTTAGAACCTGCATTAAAAGAACAACTGAAGGGTATTTTTGCCGGACTGGAGGCTGACTT
TACCTTTGATATATCCGTATCAGCCAGTCATGAAAGCCGTGCCGAATTGCTGGAATTGCTGAGCGATGTAGCCGAATGTT
CCGATCACATCACTTGTATGGTAAATGAAGGAAGCGGACTTAAATTTACGATAGGAAAGAATGGTAAACCTACAGAAATT
ACTTTCCGTGCTATCCCCAACGGACATGAGTTTACTTCTTTATTACTCGCCATCCTGAATCTGGACGGTAAGGGAAAGAA
TTTTCCGGATGAGGCTGTCTGCAACCGTGTGAAAGCCTTGAAGGGTCCGATACACTTAGTGACCTATGTCTCACTGACTT
GCACCAACTGTCCGGATGTAGTTCAGGCATTGAATGCAATGACCACACTGAATCCTTCCATTACTCATGAAATGGTTGAC
GGTGCACTGTATCAGGATGAAGTGGATGCCTTGAAGATACAAGGCGTGCCTTCTGTATTTGCCGATGGGAAACTGCTTCA
TGTAGGTCGTGGCGAATTTGGCGAATTGCTTGCTAAACTGGAAGCGCAATACGGTATTGATGAAACCAAGGTGGAGACGG
AAGTGAAGGAATACGATGTTATCGTAGCCGGTGGCGGTCCTGCCGGAGTATCAGCAGCCATCTATTCTGCCCGTAAAGGC
TTGCGCGTGGCGATTGTTGCCGAGCGTGTCGGCGGACAGGTGAAAGAGACGGTAGGCATTGAGAATCTGATTTCGGTTCC
CGAAACAACAGGAAGCGAACTTGCCGATAATCTGAAAACGCATCTTCTGCGTTATCCTGTTGATTTGTTGGAACAGCGTA
AGATAGAAAAGGTAGAACTTGCCGGAAAAGATAAACTGGTAACTACTTCCGTAGGAGAGAAATTCATTGCTCCTGCATTG
ATTATCGCTACAGGTGCAAGCTGGCGAAAACTGAATGTTCCCGGCGAGAACGATTATATCGGGCGTGGAGTCGCTTTCTG
TCCTCATTGCGACGGTCCTTTCTATAAAGGAAAGCAGGTTGCGGTAGTGGGCGGTGGAAACTCCGGCATCGAGGCAGCCA
TTGACTTGGCAGGTATCTGTTCTAAAGTCACCGTCTTTGAATTTATGGATGAACTGAAAGCGGATAATGTCCTTCAGGAA
AGACTTAAATCTCTGCCGAATGTGGAAGTATTCGTGAGTTCGCAAACGACAGAAGTAATTGGTAATGGCGATAAACTGAC
AGCCTTGCGCATCAAAGACCGTAAGACGGAAGAAGAACGTTTGGTAGAACTGGACGGAGTATTCGTGCAGATCGGACTTT
CCGCCAATAGCAGCGTTTTCCGTGATATTGTGGAAACAAACCGACCGGGTGAAATCGTTATCGATGCGCATTGCCGTACC
AATGTGACGGGAATCTATGCAGCCGGAGACGTATCGACCGTACCTTACAAACAGATTATTATTTCTATGGGCGAAGGTGC
CAAAGCCGCCCTTTCTGCCTTTGATGACCGCGTGCGGGGGCTGATTGTATAA

Upstream 100 bases:

>100_bases
CTGAAGCCAAGCATTGACCTTGTCGGTAAGATTTGATAGATCTTGATAATGTGTGTGAAGGGCCGGTAACGGAGACGCTG
CCGGCCCTTCTTTTAAAAGA

Downstream 100 bases:

>100_bases
GTAATGGCCACAGAGATTGTAAAAAGTCTCTTGTGGCTTTTATTTTTTTCTTACCTTTGTGATTCTAATATTTAACCCCC
AATGAGAAACATGAAAAAGA

Product: alkyl hydroperoxide reductase subunit F

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 523; Mature: 523

Protein sequence:

>523_residues
MKGEREMLEPALKEQLKGIFAGLEADFTFDISVSASHESRAELLELLSDVAECSDHITCMVNEGSGLKFTIGKNGKPTEI
TFRAIPNGHEFTSLLLAILNLDGKGKNFPDEAVCNRVKALKGPIHLVTYVSLTCTNCPDVVQALNAMTTLNPSITHEMVD
GALYQDEVDALKIQGVPSVFADGKLLHVGRGEFGELLAKLEAQYGIDETKVETEVKEYDVIVAGGGPAGVSAAIYSARKG
LRVAIVAERVGGQVKETVGIENLISVPETTGSELADNLKTHLLRYPVDLLEQRKIEKVELAGKDKLVTTSVGEKFIAPAL
IIATGASWRKLNVPGENDYIGRGVAFCPHCDGPFYKGKQVAVVGGGNSGIEAAIDLAGICSKVTVFEFMDELKADNVLQE
RLKSLPNVEVFVSSQTTEVIGNGDKLTALRIKDRKTEEERLVELDGVFVQIGLSANSSVFRDIVETNRPGEIVIDAHCRT
NVTGIYAAGDVSTVPYKQIIISMGEGAKAALSAFDDRVRGLIV

Sequences:

>Translated_523_residues
MKGEREMLEPALKEQLKGIFAGLEADFTFDISVSASHESRAELLELLSDVAECSDHITCMVNEGSGLKFTIGKNGKPTEI
TFRAIPNGHEFTSLLLAILNLDGKGKNFPDEAVCNRVKALKGPIHLVTYVSLTCTNCPDVVQALNAMTTLNPSITHEMVD
GALYQDEVDALKIQGVPSVFADGKLLHVGRGEFGELLAKLEAQYGIDETKVETEVKEYDVIVAGGGPAGVSAAIYSARKG
LRVAIVAERVGGQVKETVGIENLISVPETTGSELADNLKTHLLRYPVDLLEQRKIEKVELAGKDKLVTTSVGEKFIAPAL
IIATGASWRKLNVPGENDYIGRGVAFCPHCDGPFYKGKQVAVVGGGNSGIEAAIDLAGICSKVTVFEFMDELKADNVLQE
RLKSLPNVEVFVSSQTTEVIGNGDKLTALRIKDRKTEEERLVELDGVFVQIGLSANSSVFRDIVETNRPGEIVIDAHCRT
NVTGIYAAGDVSTVPYKQIIISMGEGAKAALSAFDDRVRGLIV
>Mature_523_residues
MKGEREMLEPALKEQLKGIFAGLEADFTFDISVSASHESRAELLELLSDVAECSDHITCMVNEGSGLKFTIGKNGKPTEI
TFRAIPNGHEFTSLLLAILNLDGKGKNFPDEAVCNRVKALKGPIHLVTYVSLTCTNCPDVVQALNAMTTLNPSITHEMVD
GALYQDEVDALKIQGVPSVFADGKLLHVGRGEFGELLAKLEAQYGIDETKVETEVKEYDVIVAGGGPAGVSAAIYSARKG
LRVAIVAERVGGQVKETVGIENLISVPETTGSELADNLKTHLLRYPVDLLEQRKIEKVELAGKDKLVTTSVGEKFIAPAL
IIATGASWRKLNVPGENDYIGRGVAFCPHCDGPFYKGKQVAVVGGGNSGIEAAIDLAGICSKVTVFEFMDELKADNVLQE
RLKSLPNVEVFVSSQTTEVIGNGDKLTALRIKDRKTEEERLVELDGVFVQIGLSANSSVFRDIVETNRPGEIVIDAHCRT
NVTGIYAAGDVSTVPYKQIIISMGEGAKAALSAFDDRVRGLIV

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG3634

COG function: function code O; Alkyl hydroperoxide reductase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081763, Length=521, Percent_Identity=46.6410748560461, Blast_Score=438, Evalue=1e-124,
Organism=Escherichia coli, GI1787114, Length=310, Percent_Identity=35.1612903225806, Blast_Score=164, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6321898, Length=310, Percent_Identity=33.2258064516129, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6320560, Length=308, Percent_Identity=34.7402597402597, Blast_Score=163, Evalue=6e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 56216; Mature: 56216

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGEREMLEPALKEQLKGIFAGLEADFTFDISVSASHESRAELLELLSDVAECSDHITCM
CCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEE
VNEGSGLKFTIGKNGKPTEITFRAIPNGHEFTSLLLAILNLDGKGKNFPDEAVCNRVKAL
EECCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
KGPIHLVTYVSLTCTNCPDVVQALNAMTTLNPSITHEMVDGALYQDEVDALKIQGVPSVF
CCCEEEEEEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHCCHHHHCCCCEEEECCCCCHH
ADGKLLHVGRGEFGELLAKLEAQYGIDETKVETEVKEYDVIVAGGGPAGVSAAIYSARKG
CCCEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEEECCCCCHHHHHHHHHCCC
LRVAIVAERVGGQVKETVGIENLISVPETTGSELADNLKTHLLRYPVDLLEQRKIEKVEL
CEEEEEEHHHCCHHHHHHCHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCHHEEE
AGKDKLVTTSVGEKFIAPALIIATGASWRKLNVPGENDYIGRGVAFCPHCDGPFYKGKQV
CCCCCEEEECCCHHHHCCEEEEECCCCCEEECCCCCCCCCCCCEEECCCCCCCCCCCCEE
AVVGGGNSGIEAAIDLAGICSKVTVFEFMDELKADNVLQERLKSLPNVEVFVSSQTTEVI
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEECCCEEEE
GNGDKLTALRIKDRKTEEERLVELDGVFVQIGLSANSSVFRDIVETNRPGEIVIDAHCRT
CCCCEEEEEEECCCCCHHHHHHEECCEEEEEECCCCHHHHHHHHHCCCCCEEEEECCCCC
NVTGIYAAGDVSTVPYKQIIISMGEGAKAALSAFDDRVRGLIV
CCCEEEECCCCCCCCHHHHHEECCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKGEREMLEPALKEQLKGIFAGLEADFTFDISVSASHESRAELLELLSDVAECSDHITCM
CCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEE
VNEGSGLKFTIGKNGKPTEITFRAIPNGHEFTSLLLAILNLDGKGKNFPDEAVCNRVKAL
EECCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
KGPIHLVTYVSLTCTNCPDVVQALNAMTTLNPSITHEMVDGALYQDEVDALKIQGVPSVF
CCCEEEEEEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHCCHHHHCCCCEEEECCCCCHH
ADGKLLHVGRGEFGELLAKLEAQYGIDETKVETEVKEYDVIVAGGGPAGVSAAIYSARKG
CCCEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEEECCCCCHHHHHHHHHCCC
LRVAIVAERVGGQVKETVGIENLISVPETTGSELADNLKTHLLRYPVDLLEQRKIEKVEL
CEEEEEEHHHCCHHHHHHCHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCHHEEE
AGKDKLVTTSVGEKFIAPALIIATGASWRKLNVPGENDYIGRGVAFCPHCDGPFYKGKQV
CCCCCEEEECCCHHHHCCEEEEECCCCCEEECCCCCCCCCCCCEEECCCCCCCCCCCCEE
AVVGGGNSGIEAAIDLAGICSKVTVFEFMDELKADNVLQERLKSLPNVEVFVSSQTTEVI
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEECCCEEEE
GNGDKLTALRIKDRKTEEERLVELDGVFVQIGLSANSSVFRDIVETNRPGEIVIDAHCRT
CCCCEEEEEEECCCCCHHHHHHEECCEEEEEECCCCHHHHHHHHHCCCCCEEEEECCCCC
NVTGIYAAGDVSTVPYKQIIISMGEGAKAALSAFDDRVRGLIV
CCCEEEECCCCCCCCHHHHHEECCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA