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The map label for this gene is aspA [H]

Identifier: 29348164

GI number: 29348164

Start: 3415335

End: 3416795

Strand: Reverse

Name: aspA [H]

Synonym: BT_2755

Alternate gene names: 29348164

Gene position: 3416795-3415335 (Counterclockwise)

Preceding gene: 29348168

Following gene: 29348163

Centisome position: 54.58

GC content: 45.45

Gene sequence:

>1461_bases
ATGTGTTATTGTTATAAAACAACCGATATGGAACAGAAATTATCAAAAAAAACTCGTACAGAGAGCGACTTGATAGGTAG
TCGTGAAGTGCCGGAAAGTGCATTGTATGGAGTACAGACACTCCGTGGTATTGAAAACTTTCGCATCAGCAAGTTTCATT
TGAATGAGTATCCCCTGTTTATTCAGGCATTGGCCATCACTAAAATGGGAGCTGCCGTTGCCAACCGTGAACTGGATTTA
TTGACTGAGGAACAGACGGATGCCATACTGAAGGCTTGTAAAGAAATACTGGAAGGAAAACATCATGACCAATTCCCTGT
AGATATGATACAGGGTGGAGCCGGAACCACCACCAATATGAATGCCAATGAAGTGATTGCCAACCGTGCACTGGAACTGA
TGGGACATGCACGCGGAGAGTATCACTACTGCTCGCCTAACGATCACGTCAATCGTTCGCAATCTACCAATGATGCGTAT
CCTACAGCCATTCACATTGGTCTGTATTATACCCATCTTAAATTGGTGAAACACTTTGCAACACTGATCGAGGCTTTCCG
AAAGAAAGGAGCGGAGTTTGCACACATCATCAAGATGGGACGTACCCAACTGGAAGATGCCGTACCGATGACACTAGGAC
AAACCTTTAATGGATTCGCAAGTATTTTGGAACATGAACTGAAGAATCTCGATTTTGCCGCACAGGATTTTCTGACGGTC
AACATGGGTGCGACAGCCATCGGAACCGGAATTACCGCCGAACCGGAATATGCGGAAAAGTGCATTGCTGCCCTGCGCAA
GATTACAGGACTGGATATCAAACTGGCAGATGACCTGATAGGCGCTACTTCGGATACTTCCTGCATGGTAGGATATTCTT
CGGCCATGCGGCGTGTAGCTGTCAAGATGAACAAGATTTGTAATGATCTGAGGCTATTGGCTTCCGGTCCTCGTTGCGGA
CTGGGAGAAATCAATCTGCCTGCCATGCAGCCGGGCTCTTCCATTATGCCGGGAAAGGTGAATCCTGTCATTCCGGAAGT
CATGAATCAGGTCTCTTATAAAGTGATAGGAAACGACCTTTGTGTGGCTATGAGTGGAGAGGCTGCGCAGATGGAACTGA
ATGCGATGGAACCTGTCATGGCACAATGCTGTTTTGAATCTGCGGATTTGTTGATGAACGGTTTTGATACATTGCGTACC
TTATGTATTGATGGTATCACAGCCAATGAAGAAAAATGCCGTCGGGATGTACACAACAGTATCGGAGTAGTGACCGCACT
GAATCCCGTAATTGGCTATAAACATTCTACAAAGATAGCGAAGGAGGCTCTGGAAACGGGTAAAGGAGTGTATGAGCTGG
TGCTGGAACATAACATTCTTTCCAAAGAAGACCTGGATACGATCTTGAAACCGGAAAATATGATTAAACCGGTGAAACTG
GATATTCATCCGAATCATTGA

Upstream 100 bases:

>100_bases
TTCTCTGTTTCTAAGATAAACAACAAAAAAGTTATTGAATAGTTTTTGCATATTTATTAAAACAATAACATCTCTGACTG
GTTATTATATCAACTAACGA

Downstream 100 bases:

>100_bases
TTCATAGGGGTAAAATGAGAGGCCAGCCTGTTGATATAGGCTGGTCTCTGTGTTTTGTATCATTAGTGTATTTTATGTAT
TTAATCTTGGATTCTTTTTC

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MCYCYKTTDMEQKLSKKTRTESDLIGSREVPESALYGVQTLRGIENFRISKFHLNEYPLFIQALAITKMGAAVANRELDL
LTEEQTDAILKACKEILEGKHHDQFPVDMIQGGAGTTTNMNANEVIANRALELMGHARGEYHYCSPNDHVNRSQSTNDAY
PTAIHIGLYYTHLKLVKHFATLIEAFRKKGAEFAHIIKMGRTQLEDAVPMTLGQTFNGFASILEHELKNLDFAAQDFLTV
NMGATAIGTGITAEPEYAEKCIAALRKITGLDIKLADDLIGATSDTSCMVGYSSAMRRVAVKMNKICNDLRLLASGPRCG
LGEINLPAMQPGSSIMPGKVNPVIPEVMNQVSYKVIGNDLCVAMSGEAAQMELNAMEPVMAQCCFESADLLMNGFDTLRT
LCIDGITANEEKCRRDVHNSIGVVTALNPVIGYKHSTKIAKEALETGKGVYELVLEHNILSKEDLDTILKPENMIKPVKL
DIHPNH

Sequences:

>Translated_486_residues
MCYCYKTTDMEQKLSKKTRTESDLIGSREVPESALYGVQTLRGIENFRISKFHLNEYPLFIQALAITKMGAAVANRELDL
LTEEQTDAILKACKEILEGKHHDQFPVDMIQGGAGTTTNMNANEVIANRALELMGHARGEYHYCSPNDHVNRSQSTNDAY
PTAIHIGLYYTHLKLVKHFATLIEAFRKKGAEFAHIIKMGRTQLEDAVPMTLGQTFNGFASILEHELKNLDFAAQDFLTV
NMGATAIGTGITAEPEYAEKCIAALRKITGLDIKLADDLIGATSDTSCMVGYSSAMRRVAVKMNKICNDLRLLASGPRCG
LGEINLPAMQPGSSIMPGKVNPVIPEVMNQVSYKVIGNDLCVAMSGEAAQMELNAMEPVMAQCCFESADLLMNGFDTLRT
LCIDGITANEEKCRRDVHNSIGVVTALNPVIGYKHSTKIAKEALETGKGVYELVLEHNILSKEDLDTILKPENMIKPVKL
DIHPNH
>Mature_486_residues
MCYCYKTTDMEQKLSKKTRTESDLIGSREVPESALYGVQTLRGIENFRISKFHLNEYPLFIQALAITKMGAAVANRELDL
LTEEQTDAILKACKEILEGKHHDQFPVDMIQGGAGTTTNMNANEVIANRALELMGHARGEYHYCSPNDHVNRSQSTNDAY
PTAIHIGLYYTHLKLVKHFATLIEAFRKKGAEFAHIIKMGRTQLEDAVPMTLGQTFNGFASILEHELKNLDFAAQDFLTV
NMGATAIGTGITAEPEYAEKCIAALRKITGLDIKLADDLIGATSDTSCMVGYSSAMRRVAVKMNKICNDLRLLASGPRCG
LGEINLPAMQPGSSIMPGKVNPVIPEVMNQVSYKVIGNDLCVAMSGEAAQMELNAMEPVMAQCCFESADLLMNGFDTLRT
LCIDGITANEEKCRRDVHNSIGVVTALNPVIGYKHSTKIAKEALETGKGVYELVLEHNILSKEDLDTILKPENMIKPVKL
DIHPNH

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=460, Percent_Identity=40.8695652173913, Blast_Score=329, Evalue=3e-90,
Organism=Escherichia coli, GI87082375, Length=465, Percent_Identity=53.763440860215, Blast_Score=482, Evalue=1e-137,
Organism=Escherichia coli, GI1787896, Length=462, Percent_Identity=40.6926406926407, Blast_Score=363, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17553882, Length=462, Percent_Identity=39.6103896103896, Blast_Score=337, Evalue=7e-93,
Organism=Caenorhabditis elegans, GI32565146, Length=326, Percent_Identity=38.6503067484663, Blast_Score=241, Evalue=9e-64,
Organism=Saccharomyces cerevisiae, GI6324993, Length=475, Percent_Identity=38.7368421052632, Blast_Score=333, Evalue=4e-92,
Organism=Drosophila melanogaster, GI24640177, Length=465, Percent_Identity=40.4301075268817, Blast_Score=343, Evalue=2e-94,
Organism=Drosophila melanogaster, GI24640179, Length=465, Percent_Identity=40.4301075268817, Blast_Score=343, Evalue=2e-94,
Organism=Drosophila melanogaster, GI24662684, Length=464, Percent_Identity=40.948275862069, Blast_Score=342, Evalue=3e-94,
Organism=Drosophila melanogaster, GI78710009, Length=462, Percent_Identity=39.3939393939394, Blast_Score=316, Evalue=2e-86,
Organism=Drosophila melanogaster, GI24583245, Length=463, Percent_Identity=37.1490280777538, Blast_Score=307, Evalue=1e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 53470; Mature: 53470

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCYCYKTTDMEQKLSKKTRTESDLIGSREVPESALYGVQTLRGIENFRISKFHLNEYPLF
CEEEEECCCHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHCEEEEEECCCCCHH
IQALAITKMGAAVANRELDLLTEEQTDAILKACKEILEGKHHDQFPVDMIQGGAGTTTNM
HHHHHHHHHHHHHHCCCHHHHCHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCC
NANEVIANRALELMGHARGEYHYCSPNDHVNRSQSTNDAYPTAIHIGLYYTHLKLVKHFA
CHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHH
TLIEAFRKKGAEFAHIIKMGRTQLEDAVPMTLGQTFNGFASILEHELKNLDFAAQDFLTV
HHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCEEEE
NMGATAIGTGITAEPEYAEKCIAALRKITGLDIKLADDLIGATSDTSCMVGYSSAMRRVA
ECCCHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCCEEEEHHHHHHHHH
VKMNKICNDLRLLASGPRCGLGEINLPAMQPGSSIMPGKVNPVIPEVMNQVSYKVIGNDL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEECCCE
CVAMSGEAAQMELNAMEPVMAQCCFESADLLMNGFDTLRTLCIDGITANEEKCRRDVHNS
EEEECCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
IGVVTALNPVIGYKHSTKIAKEALETGKGVYELVLEHNILSKEDLDTILKPENMIKPVKL
CCHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEEE
DIHPNH
EECCCC
>Mature Secondary Structure
MCYCYKTTDMEQKLSKKTRTESDLIGSREVPESALYGVQTLRGIENFRISKFHLNEYPLF
CEEEEECCCHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHCEEEEEECCCCCHH
IQALAITKMGAAVANRELDLLTEEQTDAILKACKEILEGKHHDQFPVDMIQGGAGTTTNM
HHHHHHHHHHHHHHCCCHHHHCHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCC
NANEVIANRALELMGHARGEYHYCSPNDHVNRSQSTNDAYPTAIHIGLYYTHLKLVKHFA
CHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHH
TLIEAFRKKGAEFAHIIKMGRTQLEDAVPMTLGQTFNGFASILEHELKNLDFAAQDFLTV
HHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCEEEE
NMGATAIGTGITAEPEYAEKCIAALRKITGLDIKLADDLIGATSDTSCMVGYSSAMRRVA
ECCCHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCCEEEEHHHHHHHHH
VKMNKICNDLRLLASGPRCGLGEINLPAMQPGSSIMPGKVNPVIPEVMNQVSYKVIGNDL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEECCCE
CVAMSGEAAQMELNAMEPVMAQCCFESADLLMNGFDTLRTLCIDGITANEEKCRRDVHNS
EEEECCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
IGVVTALNPVIGYKHSTKIAKEALETGKGVYELVLEHNILSKEDLDTILKPENMIKPVKL
CCHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEEE
DIHPNH
EECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3096982 [H]