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The map label for this gene is gltX [H]

Identifier: 29348157

GI number: 29348157

Start: 3404799

End: 3406313

Strand: Reverse

Name: gltX [H]

Synonym: BT_2748

Alternate gene names: 29348157

Gene position: 3406313-3404799 (Counterclockwise)

Preceding gene: 29348159

Following gene: 29348156

Centisome position: 54.41

GC content: 46.6

Gene sequence:

>1515_bases
ATGGCAGAAAGAAAAGTAAGAGTTCGTTTTGCTCCGAGTCCTACGGGAGCATTGCACATCGGTGGTGTGCGTACAGCTTT
GTATAATTATCTATTTGCGCGTCAACACGGCGGAGACCTCATTTTCCGTATTGAAGATACTGACTCCAACCGGTTCGTGC
CGGGAGCGGAAGAGTATATACTTGAGTCATTCAAGTGGTTGGGTATCCACTTTGACGAAGGAGTGAGCTTCGGTGGAGAA
CACGGCCCGTACCGTCAGTCCGAACGTCGCGAAATCTATAAGAAATACGTGCAGATTTTGTTGGATAATGACAAGGCATA
CATCGCTTTTGATACTCCCGAGGAACTGGACGCTAAGCGTGCGGAAATTGCTAACTTCCAATATGATGCATCGACCCGCG
GGATGATGCGTAATTCTCTGACAATGTCGAAAGAAGAAGTGGACGCATTGATTGCTGAAGGAAAGCAATATGTGGTTCGC
TTTAAGATCGAGCCGAACGAGGATGTTCACGTGAATGACTTGATTCGTGGCGAAGTCGTTATTAATTCATCTATTCTGGA
TGACAAGGTGCTTTATAAGTCGGCTGACGAACTGCCTACTTATCATCTCGCAAACATTGTGGATGACCATTTGATGGAAG
TGTCTCATGTAATCCGTGGCGAAGAATGGTTGCCTTCGGCACCGCTGCACGTACTTTTGTACCGTGCATTCGGCTGGGAA
GACACGATGCCGGAATTTGCCCACTTGCCTTTGCTGTTGAAGCCGGAAGGAAACGGTAAACTAAGCAAGCGTGACGGCGA
CCGTTTGGGATTCCCTGTATTCCCGTTGGAATGGCGTCCGGAATCGGGAGAAGTGTCTTCCGGATATCGTGAATCGGGTT
ATTTGCCCGAAGCGGTGATTAACTTCCTGGCTTTGTTGGGATGGAATCCGGGCAACGATCAGGAAGTGATGTCTATGGAC
GAGATGATAAAACTGTTCGACATTCATCGTTGCAGCAAGTCCGGAGCTAAATTCGACTATAAAAAAGGTATTTGGTTCAA
TCATCAGTATATCCAGCTTAAACCGAATGAAGAAATTGCCGAACTGTTTTTGCCTGTGTTGAAAGAGCATGGGGTAGAGG
CTCCTTTCGAGAAGGTCGTTACGGTAGTCGGCATGATGAAAGACCGTGTAAGTTTTATCAAGGAACTGTGGGATGTATGT
AGTTTCTTCTTTGTGGCTCCTGCTGAATATGACGAAAAGACAGTGAAGAAACGCTGGAAAGAAGACTCGGCAAAATGTAT
GACTGAGTTGGCGGAAGTAATTGCCGGCATCGAGGATTTCAGCATTGAAGGTCAGGAGAAAGTAGTGATGGACTGGATTG
CTGAAAAAGGTTACCATACAGGTAATATAATGAATGCTTTCCGTCTGACACTGGTAGGCGAAGGTAAAGGTCCGCATATG
TTCGATATTTCCTGGGTGTTGGGCAAGGAAGAGACATTGGCTCGTATGAAGCGCGCCGTAGAGGTACTTAAATAA

Upstream 100 bases:

>100_bases
TTCCATACGCATTGAAATTTTTGCGAAAAGTACAAAAAAAAACAATAGTGTGGAAAAAAAGGTTTAATTTTGCCACGATT
TACCTATACTAACCCAAATT

Downstream 100 bases:

>100_bases
TTAGGATTTGTAAATTATGCTTTACAATCTGGCAATAGTTATTTATGACCTTTTGGTTCATTTTGCTGCTCCGTTCAGCC
GTAAGCCCCGAAAAATGATG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 504; Mature: 503

Protein sequence:

>504_residues
MAERKVRVRFAPSPTGALHIGGVRTALYNYLFARQHGGDLIFRIEDTDSNRFVPGAEEYILESFKWLGIHFDEGVSFGGE
HGPYRQSERREIYKKYVQILLDNDKAYIAFDTPEELDAKRAEIANFQYDASTRGMMRNSLTMSKEEVDALIAEGKQYVVR
FKIEPNEDVHVNDLIRGEVVINSSILDDKVLYKSADELPTYHLANIVDDHLMEVSHVIRGEEWLPSAPLHVLLYRAFGWE
DTMPEFAHLPLLLKPEGNGKLSKRDGDRLGFPVFPLEWRPESGEVSSGYRESGYLPEAVINFLALLGWNPGNDQEVMSMD
EMIKLFDIHRCSKSGAKFDYKKGIWFNHQYIQLKPNEEIAELFLPVLKEHGVEAPFEKVVTVVGMMKDRVSFIKELWDVC
SFFFVAPAEYDEKTVKKRWKEDSAKCMTELAEVIAGIEDFSIEGQEKVVMDWIAEKGYHTGNIMNAFRLTLVGEGKGPHM
FDISWVLGKEETLARMKRAVEVLK

Sequences:

>Translated_504_residues
MAERKVRVRFAPSPTGALHIGGVRTALYNYLFARQHGGDLIFRIEDTDSNRFVPGAEEYILESFKWLGIHFDEGVSFGGE
HGPYRQSERREIYKKYVQILLDNDKAYIAFDTPEELDAKRAEIANFQYDASTRGMMRNSLTMSKEEVDALIAEGKQYVVR
FKIEPNEDVHVNDLIRGEVVINSSILDDKVLYKSADELPTYHLANIVDDHLMEVSHVIRGEEWLPSAPLHVLLYRAFGWE
DTMPEFAHLPLLLKPEGNGKLSKRDGDRLGFPVFPLEWRPESGEVSSGYRESGYLPEAVINFLALLGWNPGNDQEVMSMD
EMIKLFDIHRCSKSGAKFDYKKGIWFNHQYIQLKPNEEIAELFLPVLKEHGVEAPFEKVVTVVGMMKDRVSFIKELWDVC
SFFFVAPAEYDEKTVKKRWKEDSAKCMTELAEVIAGIEDFSIEGQEKVVMDWIAEKGYHTGNIMNAFRLTLVGEGKGPHM
FDISWVLGKEETLARMKRAVEVLK
>Mature_503_residues
AERKVRVRFAPSPTGALHIGGVRTALYNYLFARQHGGDLIFRIEDTDSNRFVPGAEEYILESFKWLGIHFDEGVSFGGEH
GPYRQSERREIYKKYVQILLDNDKAYIAFDTPEELDAKRAEIANFQYDASTRGMMRNSLTMSKEEVDALIAEGKQYVVRF
KIEPNEDVHVNDLIRGEVVINSSILDDKVLYKSADELPTYHLANIVDDHLMEVSHVIRGEEWLPSAPLHVLLYRAFGWED
TMPEFAHLPLLLKPEGNGKLSKRDGDRLGFPVFPLEWRPESGEVSSGYRESGYLPEAVINFLALLGWNPGNDQEVMSMDE
MIKLFDIHRCSKSGAKFDYKKGIWFNHQYIQLKPNEEIAELFLPVLKEHGVEAPFEKVVTVVGMMKDRVSFIKELWDVCS
FFFVAPAEYDEKTVKKRWKEDSAKCMTELAEVIAGIEDFSIEGQEKVVMDWIAEKGYHTGNIMNAFRLTLVGEGKGPHMF
DISWVLGKEETLARMKRAVEVLK

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=519, Percent_Identity=34.2967244701349, Blast_Score=251, Evalue=1e-66,
Organism=Escherichia coli, GI1788743, Length=500, Percent_Identity=31.6, Blast_Score=255, Evalue=5e-69,
Organism=Escherichia coli, GI226510925, Length=267, Percent_Identity=31.0861423220974, Blast_Score=113, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71993394, Length=518, Percent_Identity=34.5559845559846, Blast_Score=247, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6324540, Length=518, Percent_Identity=36.6795366795367, Blast_Score=296, Evalue=5e-81,
Organism=Saccharomyces cerevisiae, GI6321192, Length=220, Percent_Identity=29.5454545454545, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24665321, Length=518, Percent_Identity=36.1003861003861, Blast_Score=297, Evalue=1e-80,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 57735; Mature: 57603

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAERKVRVRFAPSPTGALHIGGVRTALYNYLFARQHGGDLIFRIEDTDSNRFVPGAEEYI
CCCCEEEEEECCCCCCEEEECHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHH
LESFKWLGIHFDEGVSFGGEHGPYRQSERREIYKKYVQILLDNDKAYIAFDTPEELDAKR
HHHHHHEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHEECCCCEEEEECCCHHHHHHH
AEIANFQYDASTRGMMRNSLTMSKEEVDALIAEGKQYVVRFKIEPNEDVHVNDLIRGEVV
HHHCCCEECCCHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCHHHEEECEEE
INSSILDDKVLYKSADELPTYHLANIVDDHLMEVSHVIRGEEWLPSAPLHVLLYRAFGWE
EECCCCCCHHHHHCHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCC
DTMPEFAHLPLLLKPEGNGKLSKRDGDRLGFPVFPLEWRPESGEVSSGYRESGYLPEAVI
CCCCHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHH
NFLALLGWNPGNDQEVMSMDEMIKLFDIHRCSKSGAKFDYKKGIWFNHQYIQLKPNEEIA
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCEEEEECCCHHHH
ELFLPVLKEHGVEAPFEKVVTVVGMMKDRVSFIKELWDVCSFFFVAPAEYDEKTVKKRWK
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHH
EDSAKCMTELAEVIAGIEDFSIEGQEKVVMDWIAEKGYHTGNIMNAFRLTLVGEGKGPHM
HHHHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHCCCCCCCHHEEEEEEEEECCCCCEE
FDISWVLGKEETLARMKRAVEVLK
EEEEEECCCHHHHHHHHHHHHHHC
>Mature Secondary Structure 
AERKVRVRFAPSPTGALHIGGVRTALYNYLFARQHGGDLIFRIEDTDSNRFVPGAEEYI
CCCEEEEEECCCCCCEEEECHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHH
LESFKWLGIHFDEGVSFGGEHGPYRQSERREIYKKYVQILLDNDKAYIAFDTPEELDAKR
HHHHHHEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHEECCCCEEEEECCCHHHHHHH
AEIANFQYDASTRGMMRNSLTMSKEEVDALIAEGKQYVVRFKIEPNEDVHVNDLIRGEVV
HHHCCCEECCCHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCHHHEEECEEE
INSSILDDKVLYKSADELPTYHLANIVDDHLMEVSHVIRGEEWLPSAPLHVLLYRAFGWE
EECCCCCCHHHHHCHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCC
DTMPEFAHLPLLLKPEGNGKLSKRDGDRLGFPVFPLEWRPESGEVSSGYRESGYLPEAVI
CCCCHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHH
NFLALLGWNPGNDQEVMSMDEMIKLFDIHRCSKSGAKFDYKKGIWFNHQYIQLKPNEEIA
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCEEEEECCCHHHH
ELFLPVLKEHGVEAPFEKVVTVVGMMKDRVSFIKELWDVCSFFFVAPAEYDEKTVKKRWK
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHH
EDSAKCMTELAEVIAGIEDFSIEGQEKVVMDWIAEKGYHTGNIMNAFRLTLVGEGKGPHM
HHHHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHCCCCCCCHHEEEEEEEEECCCCCEE
FDISWVLGKEETLARMKRAVEVLK
EEEEEECCCHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA