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The map label for this gene is waaA [H]

Identifier: 29348156

GI number: 29348156

Start: 3403559

End: 3404782

Strand: Reverse

Name: waaA [H]

Synonym: BT_2747

Alternate gene names: 29348156

Gene position: 3404782-3403559 (Counterclockwise)

Preceding gene: 29348157

Following gene: 29348151

Centisome position: 54.39

GC content: 43.87

Gene sequence:

>1224_bases
ATGCTTTACAATCTGGCAATAGTTATTTATGACCTTTTGGTTCATTTTGCTGCTCCGTTCAGCCGTAAGCCCCGAAAAAT
GATGAAAGGGCATTGGGTGGTGTATGAACTTCTACGACAGCAAGTGGAGAAAGGGGAGCAGTATATTTGGTTTCACGCTG
CTTCGCTTGGCGAGTTTGAACAAGGGCGTCCATTAATAGAAATGATCAGGGAAAAATATCCCGATTATAAGATATTGCTT
ACATTTTTCTCTCCGTCCGGTTACGAAGTGCGCAAGCATTACCGGGGAGCGGACATTGTCTGCTATCTGCCGTTTGATAA
ACCGCGGAATGTGAAGAAGTTCCTGGATATTGCAAACCCGTGCATGGCATTCTTTATCAAGTACGAATTTTGGAAGAATT
ACCTGGATGAGCTTCATAAGCGTCGCATCCCGGTGTATAGTGTTTCTTCCATTTTCCGTCGCGATCAGATATTCTTTAAA
TGGTATGGTGGGACTTATCGTAATGTGCTGAAGGATTTCGATCATTTGTTTGTGCAGAATGAGGCTTCCAAACGCTATTT
GTCGAAGATTGGCATTTGTCGTGTGACAGTGGTGGGAGATACCCGTTTCGACCGTGTGTTGCAGATTCGGGAAGAGGCCA
AAGACCTTCCGTTGGTGAAAATGTTTAAAGGAGACAATGCCTTTACGTTTGTGGCAGGCAGTTCTTGGGGACCGGATGAA
GATTTATTCCTGGAGTACTTCAACACCCATCCGGAAATGAAGTTGATCATCGCTCCGCATGTGATTGATGAAAATCATCT
GGTGGAGATTATCAGTAAACTGAAACGTCCTTATGTACGCTATACTCGTGCTGACGAAAAGAATGTACTGAAAGTTGATT
GCTTGATTATCGATTGCTTCGGACTGTTATCTTCTATTTACCGCTATGGAGAAGTGGCTTATATCGGAGGTGGTTTTGGC
GTCGGTATTCATAATACGCTGGAAGCTGCTGTATATGGCATCCCTGTTATCTTCGGACCGAAATATCAGAAGTTTATGGA
AGCAATCCGGTTGCTCGAAGCTAAAGGCGCATACTCAATTAAAGACTATCACGAGTTGAAAACCTTGCTGGACCGTTTTC
AGGCAGATGATGTTTTCATGCGTGAGACCGGTGCGAATGCTGGTTATTATGTGACCAGTAATGCCGGAGCTACGGAGAAG
ATTATGCACATGATTAACTTTTAG

Upstream 100 bases:

>100_bases
CCGCATATGTTCGATATTTCCTGGGTGTTGGGCAAGGAAGAGACATTGGCTCGTATGAAGCGCGCCGTAGAGGTACTTAA
ATAATTAGGATTTGTAAATT

Downstream 100 bases:

>100_bases
AGATGGATAAGGTTGGGCGTCGCCTGAAAAACAGGTAAGCCCTATCATTTCGCTCTGCTGTTCTGAGCTTGTCGTCTCGT
TTTTCTTAAAGAAGAGAGAT

Product: 3-deoxy-D-manno-octulosonic-acid transferase

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MLYNLAIVIYDLLVHFAAPFSRKPRKMMKGHWVVYELLRQQVEKGEQYIWFHAASLGEFEQGRPLIEMIREKYPDYKILL
TFFSPSGYEVRKHYRGADIVCYLPFDKPRNVKKFLDIANPCMAFFIKYEFWKNYLDELHKRRIPVYSVSSIFRRDQIFFK
WYGGTYRNVLKDFDHLFVQNEASKRYLSKIGICRVTVVGDTRFDRVLQIREEAKDLPLVKMFKGDNAFTFVAGSSWGPDE
DLFLEYFNTHPEMKLIIAPHVIDENHLVEIISKLKRPYVRYTRADEKNVLKVDCLIIDCFGLLSSIYRYGEVAYIGGGFG
VGIHNTLEAAVYGIPVIFGPKYQKFMEAIRLLEAKGAYSIKDYHELKTLLDRFQADDVFMRETGANAGYYVTSNAGATEK
IMHMINF

Sequences:

>Translated_407_residues
MLYNLAIVIYDLLVHFAAPFSRKPRKMMKGHWVVYELLRQQVEKGEQYIWFHAASLGEFEQGRPLIEMIREKYPDYKILL
TFFSPSGYEVRKHYRGADIVCYLPFDKPRNVKKFLDIANPCMAFFIKYEFWKNYLDELHKRRIPVYSVSSIFRRDQIFFK
WYGGTYRNVLKDFDHLFVQNEASKRYLSKIGICRVTVVGDTRFDRVLQIREEAKDLPLVKMFKGDNAFTFVAGSSWGPDE
DLFLEYFNTHPEMKLIIAPHVIDENHLVEIISKLKRPYVRYTRADEKNVLKVDCLIIDCFGLLSSIYRYGEVAYIGGGFG
VGIHNTLEAAVYGIPVIFGPKYQKFMEAIRLLEAKGAYSIKDYHELKTLLDRFQADDVFMRETGANAGYYVTSNAGATEK
IMHMINF
>Mature_407_residues
MLYNLAIVIYDLLVHFAAPFSRKPRKMMKGHWVVYELLRQQVEKGEQYIWFHAASLGEFEQGRPLIEMIREKYPDYKILL
TFFSPSGYEVRKHYRGADIVCYLPFDKPRNVKKFLDIANPCMAFFIKYEFWKNYLDELHKRRIPVYSVSSIFRRDQIFFK
WYGGTYRNVLKDFDHLFVQNEASKRYLSKIGICRVTVVGDTRFDRVLQIREEAKDLPLVKMFKGDNAFTFVAGSSWGPDE
DLFLEYFNTHPEMKLIIAPHVIDENHLVEIISKLKRPYVRYTRADEKNVLKVDCLIIDCFGLLSSIYRYGEVAYIGGGFG
VGIHNTLEAAVYGIPVIFGPKYQKFMEAIRLLEAKGAYSIKDYHELKTLLDRFQADDVFMRETGANAGYYVTSNAGATEK
IMHMINF

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=369, Percent_Identity=25.7452574525745, Blast_Score=132, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 47625; Mature: 47625

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYNLAIVIYDLLVHFAAPFSRKPRKMMKGHWVVYELLRQQVEKGEQYIWFHAASLGEFE
CEEHHHHHHHHHHHHHCCCCCCCCHHHHHCHHHHHHHHHHHHHCCCEEEEEEECCCCCCC
QGRPLIEMIREKYPDYKILLTFFSPSGYEVRKHYRGADIVCYLPFDKPRNVKKFLDIANP
CCCHHHHHHHHHCCCEEEEEEEECCCCHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHH
CMAFFIKYEFWKNYLDELHKRRIPVYSVSSIFRRDQIFFKWYGGTYRNVLKDFDHLFVQN
HHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCC
EASKRYLSKIGICRVTVVGDTRFDRVLQIREEAKDLPLVKMFKGDNAFTFVAGSSWGPDE
HHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCCH
DLFLEYFNTHPEMKLIIAPHVIDENHLVEIISKLKRPYVRYTRADEKNVLKVDCLIIDCF
HHHHHHHCCCCCEEEEEECEEECCHHHHHHHHHHCCCCEEHCCCCCCCEEEEHHHHHHHH
GLLSSIYRYGEVAYIGGGFGVGIHNTLEAAVYGIPVIFGPKYQKFMEAIRLLEAKGAYSI
HHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHCCCCCCH
KDYHELKTLLDRFQADDVFMRETGANAGYYVTSNAGATEKIMHMINF
HHHHHHHHHHHHHCCCCEEEECCCCCCCEEEECCCCHHHHHHHHHCC
>Mature Secondary Structure
MLYNLAIVIYDLLVHFAAPFSRKPRKMMKGHWVVYELLRQQVEKGEQYIWFHAASLGEFE
CEEHHHHHHHHHHHHHCCCCCCCCHHHHHCHHHHHHHHHHHHHCCCEEEEEEECCCCCCC
QGRPLIEMIREKYPDYKILLTFFSPSGYEVRKHYRGADIVCYLPFDKPRNVKKFLDIANP
CCCHHHHHHHHHCCCEEEEEEEECCCCHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHH
CMAFFIKYEFWKNYLDELHKRRIPVYSVSSIFRRDQIFFKWYGGTYRNVLKDFDHLFVQN
HHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCC
EASKRYLSKIGICRVTVVGDTRFDRVLQIREEAKDLPLVKMFKGDNAFTFVAGSSWGPDE
HHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCCH
DLFLEYFNTHPEMKLIIAPHVIDENHLVEIISKLKRPYVRYTRADEKNVLKVDCLIIDCF
HHHHHHHCCCCCEEEEEECEEECCHHHHHHHHHHCCCCEEHCCCCCCCEEEEHHHHHHHH
GLLSSIYRYGEVAYIGGGFGVGIHNTLEAAVYGIPVIFGPKYQKFMEAIRLLEAKGAYSI
HHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHCCCCCCH
KDYHELKTLLDRFQADDVFMRETGANAGYYVTSNAGATEKIMHMINF
HHHHHHHHHHHHHCCCCEEEECCCCCCCEEEECCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]