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The map label for this gene is ykfC [H]

Identifier: 29348025

GI number: 29348025

Start: 3254829

End: 3256121

Strand: Reverse

Name: ykfC [H]

Synonym: BT_2615

Alternate gene names: 29348025

Gene position: 3256121-3254829 (Counterclockwise)

Preceding gene: 29348027

Following gene: 29348019

Centisome position: 52.01

GC content: 43.62

Gene sequence:

>1293_bases
ATGCAAAACGACAACGCAAAACCAATATCAATATCGAAGCAATTGGTTTATGATGCATTTCTGCGTGTGAAAGCAAACCG
TGGAAGTGCAGGGATTGATAAGGTCACTCTTGAAGATTATGAAAAGAATCTGCGAGGTAACCTTTATAAACTATGGAATC
GCATGAGTTCCGGCAGTTACTTTCCTCCATCGGTGAAGCTGGTAGAAATACCGAAGAGTACTGGTGGTAAACGTCCATTG
GGTATCCCGACGGTATCTGATCGTGTAGCTCAAATGGCTGTAGTAATGCTGATAACCCCTTCTATCGAACCTTGTTTTCA
TGAGGATTCTTATGCCTACCGTCCCCATCGCTCGGCGCATGATGCTGTTGGCAAAGCTCGTGAGCGTTGTTGGAAGTATG
CGTGGGTTCTGGACATGGACATCAGTAAATTCTTTGACACCATAGACCATGAACTACTGCTGAAAGCCTTGAAACGGCAT
ACGCAAGAAAAATGGGTATTGATGTATATAGAACGTTGGCTGAAAGTTCCTTATGAGAAATCGGATGGAAGTCAGGTTGA
CAGGGCTCTCGGTGTACCCCAAGGCAGTGTCATAGGTCCGGTTCTTGCGAATCTGTTTCTTCATTATACCTTTGACAAGT
GGATGGAAAAGAACTTTCCCCGTGTTCCATTTGAGAGATATGCTGATGATACAATTTGTCACTGCCATAGTCTTAAACAG
GCTGAGTATATGCAAGCGATGATTCAACAACGGTTTGAGTGCTGCAGGTTGCGGCTCAATGAAGAGAAAACGAAAATCGT
CTATTGCAAAAGTAGCCGTCAAAAGGAGTGCTATCCCAATGTGACTTTTGACTTTCTTGGATTTACGTTTCAACCCCGGG
AATCGGTAGATAAGTATGGCAACCGTTTTACGGGTTTCCTGCCGGCTATCAGCCGAAAATCAATGAAACGAATCAATGAG
ACGATGCGCAGTTGGCACCTAAACAGGCACTCAAATCTCACATTGGAACACCTTGCGTCTGATATAAATCCTATCGTACG
AGGTTGGATGACTTATTATGGTAAGTTTTATCCTACCCGTTTGAAGTGGTTTATGCAGACGTTGAACGGACGTCTAGCCC
GTTGGGTAATGTGTAAGTTCGAGCGTTATAGACATCGTTTCTATCCTGCTCAAGAATGGCTTGCCCGTATTGCAGAGAAA
GAGGGGCTTATCTTTTATCATTGGAAATGTGGCGCTCTGCCAAGATTTACCAATAAAGAAAAGGTGAGTTCCCAATTGAT
AATGGTTAAATAA

Upstream 100 bases:

>100_bases
ACTGACTCGTAGTAGTGTAGAAGTGCCTGTAATGGGTATGGAGCGAAGGGGTCAGCTTATTTAGCGTAAAAGTTAGTACA
ACTGATAAACAATTAGGAAG

Downstream 100 bases:

>100_bases
GTAAAGCCGTGTGAAGCGAGAGTTTCACGCACGGTTTATTGAGAAGCTCGGGGTGAAACTCCCCTTGCTTACTCGGCGGT
CGGAAAACGAAAAAGTAGGA

Product: reverse transcriptase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MQNDNAKPISISKQLVYDAFLRVKANRGSAGIDKVTLEDYEKNLRGNLYKLWNRMSSGSYFPPSVKLVEIPKSTGGKRPL
GIPTVSDRVAQMAVVMLITPSIEPCFHEDSYAYRPHRSAHDAVGKARERCWKYAWVLDMDISKFFDTIDHELLLKALKRH
TQEKWVLMYIERWLKVPYEKSDGSQVDRALGVPQGSVIGPVLANLFLHYTFDKWMEKNFPRVPFERYADDTICHCHSLKQ
AEYMQAMIQQRFECCRLRLNEEKTKIVYCKSSRQKECYPNVTFDFLGFTFQPRESVDKYGNRFTGFLPAISRKSMKRINE
TMRSWHLNRHSNLTLEHLASDINPIVRGWMTYYGKFYPTRLKWFMQTLNGRLARWVMCKFERYRHRFYPAQEWLARIAEK
EGLIFYHWKCGALPRFTNKEKVSSQLIMVK

Sequences:

>Translated_430_residues
MQNDNAKPISISKQLVYDAFLRVKANRGSAGIDKVTLEDYEKNLRGNLYKLWNRMSSGSYFPPSVKLVEIPKSTGGKRPL
GIPTVSDRVAQMAVVMLITPSIEPCFHEDSYAYRPHRSAHDAVGKARERCWKYAWVLDMDISKFFDTIDHELLLKALKRH
TQEKWVLMYIERWLKVPYEKSDGSQVDRALGVPQGSVIGPVLANLFLHYTFDKWMEKNFPRVPFERYADDTICHCHSLKQ
AEYMQAMIQQRFECCRLRLNEEKTKIVYCKSSRQKECYPNVTFDFLGFTFQPRESVDKYGNRFTGFLPAISRKSMKRINE
TMRSWHLNRHSNLTLEHLASDINPIVRGWMTYYGKFYPTRLKWFMQTLNGRLARWVMCKFERYRHRFYPAQEWLARIAEK
EGLIFYHWKCGALPRFTNKEKVSSQLIMVK
>Mature_430_residues
MQNDNAKPISISKQLVYDAFLRVKANRGSAGIDKVTLEDYEKNLRGNLYKLWNRMSSGSYFPPSVKLVEIPKSTGGKRPL
GIPTVSDRVAQMAVVMLITPSIEPCFHEDSYAYRPHRSAHDAVGKARERCWKYAWVLDMDISKFFDTIDHELLLKALKRH
TQEKWVLMYIERWLKVPYEKSDGSQVDRALGVPQGSVIGPVLANLFLHYTFDKWMEKNFPRVPFERYADDTICHCHSLKQ
AEYMQAMIQQRFECCRLRLNEEKTKIVYCKSSRQKECYPNVTFDFLGFTFQPRESVDKYGNRFTGFLPAISRKSMKRINE
TMRSWHLNRHSNLTLEHLASDINPIVRGWMTYYGKFYPTRLKWFMQTLNGRLARWVMCKFERYRHRFYPAQEWLARIAEK
EGLIFYHWKCGALPRFTNKEKVSSQLIMVK

Specific function: Unknown

COG id: COG3344

COG function: function code L; Retron-type reverse transcriptase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 reverse transcriptase domain [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6226520, Length=198, Percent_Identity=29.7979797979798, Blast_Score=106, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6226521, Length=208, Percent_Identity=27.8846153846154, Blast_Score=104, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015706
- InterPro:   IPR000477 [H]

Pfam domain/function: PF00078 RVT_1 [H]

EC number: NA

Molecular weight: Translated: 50758; Mature: 50758

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS50878 RT_POL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHCCCEEEEC
>Mature Secondary Structure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HHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]