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The map label for this gene is bglA [H]

Identifier: 29347960

GI number: 29347960

Start: 3182742

End: 3183548

Strand: Reverse

Name: bglA [H]

Synonym: BT_2550

Alternate gene names: 29347960

Gene position: 3183548-3182742 (Counterclockwise)

Preceding gene: 29347963

Following gene: 29347959

Centisome position: 50.85

GC content: 47.34

Gene sequence:

>807_bases
ATGAAACAGTTCAGAAATCTATTATTTATTAGCACATTACTCCTTTGTTGGGTACTCGTTTCCTGCAAAACGACAAGAGT
TTCTTCCTCCGGCTGGTCGCTGGTATGGGAAGAGAATTTTAATCAGAAAAAAGGCTTTGACCCGCAGGTCTGGAGCAAGA
TTCCGCGAGGGAAGGCCGACTGGAATAATTATATGACAGACTTTGATTCCTGCTTTGCCATGCGCAAAGGCAAGCTGGTG
TTAAGAGGCATTGCCAATCAGACGCTGCCCAATGATACGGCACCTTATCTGACAGGAGGCGTTTATACCAAAGGTAAAAA
GGCATTCCTTGACGGACGCATCGAGATATGCGCCAAGCTGAATGCCGCAAAAGGAGCATGGCCGGCTATCTGGCTGTTGC
CCGAAAATGCCAAATGGCCGTCGGGAGGCGAAATAGATGTGATGGAGCGTCTGAATGACGACTCTATCGCTTATCAGACA
GTTCATTCTCATTATACGTACACTTTGGGCATCAAAGAGCACCCCAAGTCACACTTCACAGGAGTCATCCATCCCGATCG
CTATAACGTTTTCGCTGTAGAAATGTATCCGGACAGTCTTTCGTTCTATGTCAACGACATACATACGTTTACTTATCCCC
GCATACAGACGCAGAAGGAAGGACAATTCCCGTTCGACCAGCCGTTTTATCTGCTGATCGATATGCAGTTGGGCGGTTCG
TGGGTCGGAGTAGTCGATCCCAAAGACCTTCCGGTAGAAATGGAAGTGGACTGGGTGCGTTTTTATCAGCGAAAGTCACT
CAAATAA

Upstream 100 bases:

>100_bases
AACATTTTGTTATATTTTAGTTGTTTTAGTGTGTAGATATACACCGAACCGTTTTTTCCTGTATTTTTGCAAATGAATCC
CCCTTAAACTTTAAAACAAA

Downstream 100 bases:

>100_bases
AACATTAAATATGAATATTCAACTTCCTTCTTTTTTACAGAAAGGTGACAAAGTAGTGATTGTGTCACCTTCCAGCAAGA
TAGACAAAGAGTTTCTGAAA

Product: beta-glucanase

Products: NA

Alternate protein names: 1,3-1,4-beta-D-glucan 4-glucanohydrolase; Endo-beta-1,3-1,4 glucanase; Lichenase [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MKQFRNLLFISTLLLCWVLVSCKTTRVSSSGWSLVWEENFNQKKGFDPQVWSKIPRGKADWNNYMTDFDSCFAMRKGKLV
LRGIANQTLPNDTAPYLTGGVYTKGKKAFLDGRIEICAKLNAAKGAWPAIWLLPENAKWPSGGEIDVMERLNDDSIAYQT
VHSHYTYTLGIKEHPKSHFTGVIHPDRYNVFAVEMYPDSLSFYVNDIHTFTYPRIQTQKEGQFPFDQPFYLLIDMQLGGS
WVGVVDPKDLPVEMEVDWVRFYQRKSLK

Sequences:

>Translated_268_residues
MKQFRNLLFISTLLLCWVLVSCKTTRVSSSGWSLVWEENFNQKKGFDPQVWSKIPRGKADWNNYMTDFDSCFAMRKGKLV
LRGIANQTLPNDTAPYLTGGVYTKGKKAFLDGRIEICAKLNAAKGAWPAIWLLPENAKWPSGGEIDVMERLNDDSIAYQT
VHSHYTYTLGIKEHPKSHFTGVIHPDRYNVFAVEMYPDSLSFYVNDIHTFTYPRIQTQKEGQFPFDQPFYLLIDMQLGGS
WVGVVDPKDLPVEMEVDWVRFYQRKSLK
>Mature_268_residues
MKQFRNLLFISTLLLCWVLVSCKTTRVSSSGWSLVWEENFNQKKGFDPQVWSKIPRGKADWNNYMTDFDSCFAMRKGKLV
LRGIANQTLPNDTAPYLTGGVYTKGKKAFLDGRIEICAKLNAAKGAWPAIWLLPENAKWPSGGEIDVMERLNDDSIAYQT
VHSHYTYTLGIKEHPKSHFTGVIHPDRYNVFAVEMYPDSLSFYVNDIHTFTYPRIQTQKEGQFPFDQPFYLLIDMQLGGS
WVGVVDPKDLPVEMEVDWVRFYQRKSLK

Specific function: Shows activity on lichenan, beta-glucan and laminarin but not on CMC cellulose or xylan [H]

COG id: COG2273

COG function: function code G; Beta-glucanase/Beta-glucan synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 16 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008985
- InterPro:   IPR013320
- InterPro:   IPR000757
- InterPro:   IPR008263 [H]

Pfam domain/function: PF00722 Glyco_hydro_16 [H]

EC number: =3.2.1.73 [H]

Molecular weight: Translated: 30912; Mature: 30912

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00284 SERPIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQFRNLLFISTLLLCWVLVSCKTTRVSSSGWSLVWEENFNQKKGFDPQVWSKIPRGKAD
CCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEECCCCCCCCCCHHHHHHCCCCCCC
WNNYMTDFDSCFAMRKGKLVLRGIANQTLPNDTAPYLTGGVYTKGKKAFLDGRIEICAKL
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCEECCEEECCCEEECCCCEEEEEEC
NAAKGAWPAIWLLPENAKWPSGGEIDVMERLNDDSIAYQTVHSHYTYTLGIKEHPKSHFT
CCCCCCCCEEEEEECCCCCCCCCCCHHHHHCCCCCEEEEEEECCEEEEEEECCCCHHHCC
GVIHPDRYNVFAVEMYPDSLSFYVNDIHTFTYPRIQTQKEGQFPFDQPFYLLIDMQLGGS
EEECCCCCCEEEEEEECCCEEEEEEEEEEEECCCEECCCCCCCCCCCCEEEEEEEEECCE
WVGVVDPKDLPVEMEVDWVRFYQRKSLK
EEEEECCCCCCEEEEHHHHHHHHHHCCC
>Mature Secondary Structure
MKQFRNLLFISTLLLCWVLVSCKTTRVSSSGWSLVWEENFNQKKGFDPQVWSKIPRGKAD
CCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEECCCCCCCCCCHHHHHHCCCCCCC
WNNYMTDFDSCFAMRKGKLVLRGIANQTLPNDTAPYLTGGVYTKGKKAFLDGRIEICAKL
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCEECCEEECCCEEECCCCEEEEEEC
NAAKGAWPAIWLLPENAKWPSGGEIDVMERLNDDSIAYQTVHSHYTYTLGIKEHPKSHFT
CCCCCCCCEEEEEECCCCCCCCCCCHHHHHCCCCCEEEEEEECCEEEEEEECCCCHHHCC
GVIHPDRYNVFAVEMYPDSLSFYVNDIHTFTYPRIQTQKEGQFPFDQPFYLLIDMQLGGS
EEECCCCCCEEEEEEECCCEEEEEEEEEEEECCCEECCCCCCCCCCCCEEEEEEEEECCE
WVGVVDPKDLPVEMEVDWVRFYQRKSLK
EEEEECCCCCCEEEEHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7925416 [H]