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The map label for this gene is gcvH

Identifier: 29347929

GI number: 29347929

Start: 3143795

End: 3144175

Strand: Reverse

Name: gcvH

Synonym: BT_2519

Alternate gene names: 29347929

Gene position: 3144175-3143795 (Counterclockwise)

Preceding gene: 29347930

Following gene: 29347928

Centisome position: 50.22

GC content: 48.29

Gene sequence:

>381_bases
ATGAACTTTCCACAGAACTTGAAGTACACGAATGAACACGAGTGGATTCGCGTAGAAGGAGACATCGCTTACGTAGGTAT
CACAGATTACGCACAGGAACAACTGGGCGATATTGTATTCGTTGATATTCCTACCGTAGGAGAAACGTTGGAAGCCGGAG
AAACTTTCGGAACGATTGAAGTGGTAAAGACCATTTCCGATCTTTTCTTACCGTTGGCAGGCGAGATACTCGAACAGAAC
GAAGCTTTGGAAGAAAATCCGGAGCTGGTAAACAAAGACCCTTACGGCGAAGGCTGGCTGATCAAGATGAAGCCTGCTGA
TGCCAGTGCTGCGGAAGACCTGCTCGATGCCGAAGCATACAAGGCAGTGGTCAACGGCTGA

Upstream 100 bases:

>100_bases
CGGGCTGGTCTGCTCCTTTTTGATACTCCATCCGGCGTACAACCTTATATTCAGAGTATTTTTATTTTAAAAACAATATA
AATCCTAAAAACTAATTATT

Downstream 100 bases:

>100_bases
ATTTGAATAAGCAGTACTTCTGCCTCTCCCCGTAGGGGGAGGTGGTAGGTAAATGAATGTATGTAATAACTTTTAGATTA
GATGAAAATGACTCCAATTG

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 126; Mature: 126

Protein sequence:

>126_residues
MNFPQNLKYTNEHEWIRVEGDIAYVGITDYAQEQLGDIVFVDIPTVGETLEAGETFGTIEVVKTISDLFLPLAGEILEQN
EALEENPELVNKDPYGEGWLIKMKPADASAAEDLLDAEAYKAVVNG

Sequences:

>Translated_126_residues
MNFPQNLKYTNEHEWIRVEGDIAYVGITDYAQEQLGDIVFVDIPTVGETLEAGETFGTIEVVKTISDLFLPLAGEILEQN
EALEENPELVNKDPYGEGWLIKMKPADASAAEDLLDAEAYKAVVNG
>Mature_126_residues
MNFPQNLKYTNEHEWIRVEGDIAYVGITDYAQEQLGDIVFVDIPTVGETLEAGETFGTIEVVKTISDLFLPLAGEILEQN
EALEENPELVNKDPYGEGWLIKMKPADASAAEDLLDAEAYKAVVNG

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=119, Percent_Identity=47.0588235294118, Blast_Score=119, Evalue=5e-28,
Organism=Homo sapiens, GI89057342, Length=119, Percent_Identity=46.218487394958, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI1789271, Length=124, Percent_Identity=53.2258064516129, Blast_Score=137, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17507493, Length=112, Percent_Identity=44.6428571428571, Blast_Score=112, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17551294, Length=121, Percent_Identity=42.1487603305785, Blast_Score=110, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6319272, Length=125, Percent_Identity=44.8, Blast_Score=113, Evalue=8e-27,
Organism=Drosophila melanogaster, GI17865652, Length=120, Percent_Identity=40, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_BACTN (Q8A4S8)

Other databases:

- EMBL:   AE015928
- RefSeq:   NP_811432.1
- ProteinModelPortal:   Q8A4S8
- SMR:   Q8A4S8
- GeneID:   1075649
- GenomeReviews:   AE015928_GR
- KEGG:   bth:BT_2519
- NMPDR:   fig|226186.1.peg.2519
- HOGENOM:   HBG693789
- OMA:   TSDHEWL
- PhylomeDB:   Q8A4S8
- ProtClustDB:   PRK01202
- BioCyc:   BTHE226186:BT_2519-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 13918; Mature: 13918

Theoretical pI: Translated: 3.71; Mature: 3.71

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFPQNLKYTNEHEWIRVEGDIAYVGITDYAQEQLGDIVFVDIPTVGETLEAGETFGTIE
CCCCCCCCCCCCCCEEEEECCEEEEECCHHHHHHHCCEEEEECCCCCCHHHCCCCCCHHH
VVKTISDLFLPLAGEILEQNEALEENPELVNKDPYGEGWLIKMKPADASAAEDLLDAEAY
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHH
KAVVNG
HHHCCC
>Mature Secondary Structure
MNFPQNLKYTNEHEWIRVEGDIAYVGITDYAQEQLGDIVFVDIPTVGETLEAGETFGTIE
CCCCCCCCCCCCCCEEEEECCEEEEECCHHHHHHHCCEEEEECCCCCCHHHCCCCCCHHH
VVKTISDLFLPLAGEILEQNEALEENPELVNKDPYGEGWLIKMKPADASAAEDLLDAEAY
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHH
KAVVNG
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928