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The map label for this gene is vat [H]

Identifier: 29347794

GI number: 29347794

Start: 2972468

End: 2973094

Strand: Reverse

Name: vat [H]

Synonym: BT_2384

Alternate gene names: 29347794

Gene position: 2973094-2972468 (Counterclockwise)

Preceding gene: 29347796

Following gene: 29347791

Centisome position: 47.49

GC content: 40.83

Gene sequence:

>627_bases
ATGAATACAAATATTTATCCGCGCACCAATGACCTTCAGACCGTCTATCTGAATGCAGTTATCAATAATCCTCATATAAA
GGTTGGTGACTACACCATATACAATGATTTTGTGAATGATCCTGTTCAGTTTGAGAAGAACAATGTTTTATATCATTATC
CTGTCAATGGAGACCGGCTGATCATAGGGAAATTCTGTTCGATAGCCTGTGGAGCTAAGTTTCTTTTCAACAGCGCGAAT
CATACGTTGAATTCTTTGTCGAATTATCCGTTTCCTATATTTTTTGAGGAGTGGCAGTTGGATAAGGGAAATATCACTTC
GGCATGGGATAATAAAGGGGATATAGTGATTGGAAACGATGTATGGATTGGTTATGAGGCTGTTGTCATGGCGGGTGTAC
ATATCGGCGACGGAGCGATTATTGCGTCGAGGGCAGTAGTCACCAAAGATGTACCTCCTTATACGATAGTAGGCGGAACG
CCGGCCCAAGAAATACGAAAGCGTTTTGACGAAAGTACAATTGCACAATTGCAGGAATTGCAGTGGTGGGACTGGCCTGT
TGAGAAGATATCCAAAGTTTTGCTATCTATCATGCATGGAAGAGTCGATATTCTAATGAAATTTTAA

Upstream 100 bases:

>100_bases
TGGACAATGGTGAAATCTGTTGTTCGAGTCTTATGTGGCTAAACAGGTCACCCGATATTTTATTATTTTATTTCAGAAAC
TATATAAAGAAGCAATTACA

Downstream 100 bases:

>100_bases
TCCACTATTACTGTGAGTTGCATGACACCCGGGTGTTGGTATGTCTGACACCCGGGTCTCATACATGCTGACACCCGGGT
CTCAGATTACTCTCTGTCGC

Product: acetyltransferase

Products: CoA; acetylmaltose [C]

Alternate protein names: NA

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MNTNIYPRTNDLQTVYLNAVINNPHIKVGDYTIYNDFVNDPVQFEKNNVLYHYPVNGDRLIIGKFCSIACGAKFLFNSAN
HTLNSLSNYPFPIFFEEWQLDKGNITSAWDNKGDIVIGNDVWIGYEAVVMAGVHIGDGAIIASRAVVTKDVPPYTIVGGT
PAQEIRKRFDESTIAQLQELQWWDWPVEKISKVLLSIMHGRVDILMKF

Sequences:

>Translated_208_residues
MNTNIYPRTNDLQTVYLNAVINNPHIKVGDYTIYNDFVNDPVQFEKNNVLYHYPVNGDRLIIGKFCSIACGAKFLFNSAN
HTLNSLSNYPFPIFFEEWQLDKGNITSAWDNKGDIVIGNDVWIGYEAVVMAGVHIGDGAIIASRAVVTKDVPPYTIVGGT
PAQEIRKRFDESTIAQLQELQWWDWPVEKISKVLLSIMHGRVDILMKF
>Mature_208_residues
MNTNIYPRTNDLQTVYLNAVINNPHIKVGDYTIYNDFVNDPVQFEKNNVLYHYPVNGDRLIIGKFCSIACGAKFLFNSAN
HTLNSLSNYPFPIFFEEWQLDKGNITSAWDNKGDIVIGNDVWIGYEAVVMAGVHIGDGAIIASRAVVTKDVPPYTIVGGT
PAQEIRKRFDESTIAQLQELQWWDWPVEKISKVLLSIMHGRVDILMKF

Specific function: Inactivates the A compounds of virginiamycin-like antibiotics, thus providing resistance to these antibiotics [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786664, Length=53, Percent_Identity=58.4905660377358, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00132 Hexapep [H]

EC number: 2.3.1.79 [C]

Molecular weight: Translated: 23583; Mature: 23583

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTNIYPRTNDLQTVYLNAVINNPHIKVGDYTIYNDFVNDPVQFEKNNVLYHYPVNGDRL
CCCCCCCCCCCCEEEEEEEEECCCCEEECCEEEEHHHCCCCEEECCCCEEEEECCCCCEE
IIGKFCSIACGAKFLFNSANHTLNSLSNYPFPIFFEEWQLDKGNITSAWDNKGDIVIGND
EEEEHHHHHHCHHHHHCCCCHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCEEECCC
VWIGYEAVVMAGVHIGDGAIIASRAVVTKDVPPYTIVGGTPAQEIRKRFDESTIAQLQEL
EEECCEEEEEEEEEECCCEEEEECEEEECCCCCEEEECCCCHHHHHHHCCHHHHHHHHHC
QWWDWPVEKISKVLLSIMHGRVDILMKF
CCCCCCHHHHHHHHHHHHCCCEEEEEEC
>Mature Secondary Structure
MNTNIYPRTNDLQTVYLNAVINNPHIKVGDYTIYNDFVNDPVQFEKNNVLYHYPVNGDRL
CCCCCCCCCCCCEEEEEEEEECCCCEEECCEEEEHHHCCCCEEECCCCEEEEECCCCCEE
IIGKFCSIACGAKFLFNSANHTLNSLSNYPFPIFFEEWQLDKGNITSAWDNKGDIVIGND
EEEEHHHHHHCHHHHHCCCCHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCEEECCC
VWIGYEAVVMAGVHIGDGAIIASRAVVTKDVPPYTIVGGTPAQEIRKRFDESTIAQLQEL
EEECCEEEEEEEEEECCCEEEEECEEEECCCCCEEEECCCCHHHHHHHCCHHHHHHHHHC
QWWDWPVEKISKVLLSIMHGRVDILMKF
CCCCCCHHHHHHHHHHHHCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 0.20 {glucose} 0.11 {maltose}

Km value (mM): 0.018 {acetyl-CoA}} 62 {glucose}} 40 {triethanolamine}} 90 {maltose}} [C]

Substrates: Acetyl-CoA; maltose [C]

Specific reaction: Acetyl-CoA + maltose = CoA + acetylmaltose [C]

General reaction: Acyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8344533; 3149758 [H]