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The map label for this gene is nfrA2 [H]

Identifier: 29347554

GI number: 29347554

Start: 2698955

End: 2699692

Strand: Reverse

Name: nfrA2 [H]

Synonym: BT_2144

Alternate gene names: 29347554

Gene position: 2699692-2698955 (Counterclockwise)

Preceding gene: 29347560

Following gene: 29347550

Centisome position: 43.12

GC content: 43.09

Gene sequence:

>738_bases
ATGTTTGAAACCGTAAAGAACAGAAGAACAATCCGGAAATACCAGCAAAAAGATATTGCTCCTGATTTGTTAAATGATTT
GCTCGAAACCTCTTTCCGCGCTTCTACGATGGGAGGGATGCAACTTTATAGTGTTATAGTGACTCGCGATGCAGAAATGA
AAGAGAAACTTTCTCCTGCTCATTTCAATCAGCCGATGGTAAAAGGGGCACCTGTCGTGCTTACTTTTTGTGCCGATTTC
CGCCGTTTCAGCAAGTGGTGTGAACAGAGAAACGCTGTTCCCGGCTACGATAATTTAATGTCTTTTATGAATGCGTCTAT
GGATACCTTGTTGGTTGCGCAAACCTTCTGCACGTTAGCTGAAGAGGCAGGCTTGGGTATCTGTTATCTGGGAACGACTA
CTTATAATCCGCAAATGATCATCGATACACTTCAACTGCCGAAACTGGTATTCCCTATTACGACGATCACAGTCGGCTAT
CCGGACGGTACTCCGGCCCAGGTGGACCGCTTGCCTCTCGAAGCTGCCGTGCATCAGGAAGTTTACCATGATTATACTCC
GGAAGACATCGATCGCTTGTACGAGTATAAAGAATCACTGCCGGAAAATAAACAGTTTATAGAAGAAAATAAGAAAGAAA
CTCTGGCACAAGTATTTACGGATGTACGTTATACAAAGAAAGACAATGAATTTATGTCTGAGAACTTGTTGAAAGTGCTT
CGTCAACAAGGATTCTAA

Upstream 100 bases:

>100_bases
GCTTTTTGTTCATGGCTTTAAGTTTTCCAAAAAGAAAATTAGATTATTTCATTGATATGGCAAAATAATATTTCTTTTTG
TATCTTTGCAAAAAAAAGAT

Downstream 100 bases:

>100_bases
TGATAAGTAATAGGTAATAGCTATGCGGACTGCACTTGTTTATGCAGCTTCGATATTACCTATTACTTAATACCTATTAC
TTGAATTATGCTTTCTTCTT

Product: putative oxidoreductase

Products: NA

Alternate protein names: NAD(P)H-dependent FMN reductase; NAD(P)H-dependent nitroreductase; NAD(P)H-dependent oxidoreductase [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MFETVKNRRTIRKYQQKDIAPDLLNDLLETSFRASTMGGMQLYSVIVTRDAEMKEKLSPAHFNQPMVKGAPVVLTFCADF
RRFSKWCEQRNAVPGYDNLMSFMNASMDTLLVAQTFCTLAEEAGLGICYLGTTTYNPQMIIDTLQLPKLVFPITTITVGY
PDGTPAQVDRLPLEAAVHQEVYHDYTPEDIDRLYEYKESLPENKQFIEENKKETLAQVFTDVRYTKKDNEFMSENLLKVL
RQQGF

Sequences:

>Translated_245_residues
MFETVKNRRTIRKYQQKDIAPDLLNDLLETSFRASTMGGMQLYSVIVTRDAEMKEKLSPAHFNQPMVKGAPVVLTFCADF
RRFSKWCEQRNAVPGYDNLMSFMNASMDTLLVAQTFCTLAEEAGLGICYLGTTTYNPQMIIDTLQLPKLVFPITTITVGY
PDGTPAQVDRLPLEAAVHQEVYHDYTPEDIDRLYEYKESLPENKQFIEENKKETLAQVFTDVRYTKKDNEFMSENLLKVL
RQQGF
>Mature_245_residues
MFETVKNRRTIRKYQQKDIAPDLLNDLLETSFRASTMGGMQLYSVIVTRDAEMKEKLSPAHFNQPMVKGAPVVLTFCADF
RRFSKWCEQRNAVPGYDNLMSFMNASMDTLLVAQTFCTLAEEAGLGICYLGTTTYNPQMIIDTLQLPKLVFPITTITVGY
PDGTPAQVDRLPLEAAVHQEVYHDYTPEDIDRLYEYKESLPENKQFIEENKKETLAQVFTDVRYTKKDNEFMSENLLKVL
RQQGF

Specific function: Reduces FMNH(2) to FMN, with NADPH as reductant. It also reduces nitroaromatic compounds, quinones, chromates and azo dyes. It could supply the reduced form of FMN to luciferase-like protein and contribute to the degradation of aromatic compounds [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flavin oxidoreductase frp family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=201, Percent_Identity=28.3582089552239, Blast_Score=74, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016446
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 28185; Mature: 28185

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFETVKNRRTIRKYQQKDIAPDLLNDLLETSFRASTMGGMQLYSVIVTRDAEMKEKLSPA
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHCCCH
HFNQPMVKGAPVVLTFCADFRRFSKWCEQRNAVPGYDNLMSFMNASMDTLLVAQTFCTLA
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHH
EEAGLGICYLGTTTYNPQMIIDTLQLPKLVFPITTITVGYPDGTPAQVDRLPLEAAVHQE
HHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHH
VYHDYTPEDIDRLYEYKESLPENKQFIEENKKETLAQVFTDVRYTKKDNEFMSENLLKVL
HHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
RQQGF
HHCCC
>Mature Secondary Structure
MFETVKNRRTIRKYQQKDIAPDLLNDLLETSFRASTMGGMQLYSVIVTRDAEMKEKLSPA
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHCCCH
HFNQPMVKGAPVVLTFCADFRRFSKWCEQRNAVPGYDNLMSFMNASMDTLLVAQTFCTLA
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHH
EEAGLGICYLGTTTYNPQMIIDTLQLPKLVFPITTITVGYPDGTPAQVDRLPLEAAVHQE
HHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHH
VYHDYTPEDIDRLYEYKESLPENKQFIEENKKETLAQVFTDVRYTKKDNEFMSENLLKVL
HHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
RQQGF
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]