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The map label for this gene is cca [H]

Identifier: 29347385

GI number: 29347385

Start: 2484488

End: 2485996

Strand: Reverse

Name: cca [H]

Synonym: BT_1975

Alternate gene names: 29347385

Gene position: 2485996-2484488 (Counterclockwise)

Preceding gene: 29347386

Following gene: 29347382

Centisome position: 39.71

GC content: 48.51

Gene sequence:

>1509_bases
ATGATTATGGACCGCCCGACCGGTGTTTTCATCAACTATTTAACTGTCTGTTGCCAGTCGTTAAGCATTAAAAAGATGAT
AGAGTTGACTCAAGAGGAACTGAAACAGTATTTTAGCGAACCGATCTTCGGACACATTGCCGAAACCGCTGACGCACTCG
GACTGGAGTGTTACGTGGTAGGCGGTTACGTTCGTGATATTTTTCTGCAACGTCCCTCCAAAGATATAGATGTAGTAGTA
GTAGGCAGCGGGATCGCAATGGCGGAAGCACTTGGAAAACGCTTGGGGCGTGGTGCGTACGTCTCTGTTTTCAAGAATTT
CGGTACGGCACAAGTGAAATATAAAGGTACGGAAGTCGAATTTGTAGGTGCCCGCAAAGAATCCTATCAACGGGATTCAC
GGAAGCCCATCGTGGAAGACGGAACACTGGAGGACGATCAGAACCGCCGTGACTTTACTATTAACGCGATGGCTGTCTGC
CTGAACAAAGCACGCTTTGGCGAACTGGTTGACCCGTTTGGCGGCATGGCGGACATGAAGGAAAAGACCATCCGCACACC
CCTCGATCCGGATATCACGTTCAGTGACGACCCGCTGCGCATGATGCGTTGCATTCGCTTTGCCACGCAACTGAATTTTT
ATATCGATGACGATACGTTCGAATCGCTCTGCCGTAACAAAGACCGGATTGAAATCATATCCCGTGAACGGATTGCGGAT
GAGCTGAACAAGATCATTCTTTCGCCTATCCCTTCAAAAGGCTTTGTAGAACTGGAACGAAGCGGACTGCTTCCGTTGAT
TTTTCCGGAACTGGCTGCCTTGCAGGGAGTAGAAACACGAAACGGACGGTCGCACAAGGATAACTTTTACCATACGCTGG
AAGTGCTGGACAATATCAGCAAAAAGACGGATAATCTCTGGTTGCGTTGGGCAGCTTTGCTGCACGATATCGCCAAGCCT
GCAACGAAACGTTGGGAGCCTAAAGCGGGCTGGACGTTCCATAATCATAATTTTATCGGTGAGAAGATGATTCCGAATAT
TTTCCGGAAGATGAAGTTGCCGATGAATGAGAAGATGAAGTATGTGCAGAAAATGGTCAGCTTGCACATGCGTCCCATTG
TGATAGCGGATGATGTAGTGACGGATTCTGCCGTACGCCGTCTGCTTTTTGAGGCGGGGGATGATATCGACGACTTGATG
ACACTTTGCGAAGCGGATATCACTTCGAAAAACATGGAGCGCAAGCAGCGTTTTCTGAATAACTTCCAGCTGGTTCGCCA
AAAGTTGAAAGACCTTGAAGAAAAGGACCGTGTCCGTAATTTCCAGCCTCCGGTGAGTGGAGAAGAGATCATGGAAACTT
TCGGCTTACAGCCCTGTCGTGAAGTCGGTGCTTTGAAAAGTGCGATAAAGGATGCTATTCTGGATGGAGTTATCCCCAAT
GAGTATGAGGCAGCCCATGCTTTTATGCTGGAACGGGCGGCGAAGATGGGACTGAAGCCTGTAAAATAG

Upstream 100 bases:

>100_bases
TCGCCCGTTCATCGTTTACTTTATAGCGACAAAAATACAAATATATCCCTTATTTTTGTAATTTTGCCCCCTCAAAACTT
GTATTGGCAATAAGTGTTTG

Downstream 100 bases:

>100_bases
CTTTTCTTGTTCAAATTGGGGTGACTAACAAGTCGATTCTATCTCAAATCTCCTCCTTCGGGAAGGAGGAGTGGTCGCAG
ACCGAGGTGGTAGGAGAAAT

Product: tRNA nucleotidyltransferase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MIMDRPTGVFINYLTVCCQSLSIKKMIELTQEELKQYFSEPIFGHIAETADALGLECYVVGGYVRDIFLQRPSKDIDVVV
VGSGIAMAEALGKRLGRGAYVSVFKNFGTAQVKYKGTEVEFVGARKESYQRDSRKPIVEDGTLEDDQNRRDFTINAMAVC
LNKARFGELVDPFGGMADMKEKTIRTPLDPDITFSDDPLRMMRCIRFATQLNFYIDDDTFESLCRNKDRIEIISRERIAD
ELNKIILSPIPSKGFVELERSGLLPLIFPELAALQGVETRNGRSHKDNFYHTLEVLDNISKKTDNLWLRWAALLHDIAKP
ATKRWEPKAGWTFHNHNFIGEKMIPNIFRKMKLPMNEKMKYVQKMVSLHMRPIVIADDVVTDSAVRRLLFEAGDDIDDLM
TLCEADITSKNMERKQRFLNNFQLVRQKLKDLEEKDRVRNFQPPVSGEEIMETFGLQPCREVGALKSAIKDAILDGVIPN
EYEAAHAFMLERAAKMGLKPVK

Sequences:

>Translated_502_residues
MIMDRPTGVFINYLTVCCQSLSIKKMIELTQEELKQYFSEPIFGHIAETADALGLECYVVGGYVRDIFLQRPSKDIDVVV
VGSGIAMAEALGKRLGRGAYVSVFKNFGTAQVKYKGTEVEFVGARKESYQRDSRKPIVEDGTLEDDQNRRDFTINAMAVC
LNKARFGELVDPFGGMADMKEKTIRTPLDPDITFSDDPLRMMRCIRFATQLNFYIDDDTFESLCRNKDRIEIISRERIAD
ELNKIILSPIPSKGFVELERSGLLPLIFPELAALQGVETRNGRSHKDNFYHTLEVLDNISKKTDNLWLRWAALLHDIAKP
ATKRWEPKAGWTFHNHNFIGEKMIPNIFRKMKLPMNEKMKYVQKMVSLHMRPIVIADDVVTDSAVRRLLFEAGDDIDDLM
TLCEADITSKNMERKQRFLNNFQLVRQKLKDLEEKDRVRNFQPPVSGEEIMETFGLQPCREVGALKSAIKDAILDGVIPN
EYEAAHAFMLERAAKMGLKPVK
>Mature_502_residues
MIMDRPTGVFINYLTVCCQSLSIKKMIELTQEELKQYFSEPIFGHIAETADALGLECYVVGGYVRDIFLQRPSKDIDVVV
VGSGIAMAEALGKRLGRGAYVSVFKNFGTAQVKYKGTEVEFVGARKESYQRDSRKPIVEDGTLEDDQNRRDFTINAMAVC
LNKARFGELVDPFGGMADMKEKTIRTPLDPDITFSDDPLRMMRCIRFATQLNFYIDDDTFESLCRNKDRIEIISRERIAD
ELNKIILSPIPSKGFVELERSGLLPLIFPELAALQGVETRNGRSHKDNFYHTLEVLDNISKKTDNLWLRWAALLHDIAKP
ATKRWEPKAGWTFHNHNFIGEKMIPNIFRKMKLPMNEKMKYVQKMVSLHMRPIVIADDVVTDSAVRRLLFEAGDDIDDLM
TLCEADITSKNMERKQRFLNNFQLVRQKLKDLEEKDRVRNFQPPVSGEEIMETFGLQPCREVGALKSAIKDAILDGVIPN
EYEAAHAFMLERAAKMGLKPVK

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=329, Percent_Identity=32.5227963525836, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI87081691, Length=304, Percent_Identity=26.9736842105263, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71995920, Length=195, Percent_Identity=29.2307692307692, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6321016, Length=233, Percent_Identity=35.1931330472103, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI281360112, Length=240, Percent_Identity=29.1666666666667, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI21357337, Length=240, Percent_Identity=29.1666666666667, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 57350; Mature: 57350

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMDRPTGVFINYLTVCCQSLSIKKMIELTQEELKQYFSEPIFGHIAETADALGLECYVV
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEE
GGYVRDIFLQRPSKDIDVVVVGSGIAMAEALGKRLGRGAYVSVFKNFGTAQVKYKGTEVE
CHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHCCCCEEEEECCCEEE
FVGARKESYQRDSRKPIVEDGTLEDDQNRRDFTINAMAVCLNKARFGELVDPFGGMADMK
EECCCHHHHHHHCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCHHHHH
EKTIRTPLDPDITFSDDPLRMMRCIRFATQLNFYIDDDTFESLCRNKDRIEIISRERIAD
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCEEECCHHHHHHHCCCCHHHHHHHHHHHH
ELNKIILSPIPSKGFVELERSGLLPLIFPELAALQGVETRNGRSHKDNFYHTLEVLDNIS
HHHHHHHCCCCCCCCCHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
KKTDNLWLRWAALLHDIAKPATKRWEPKAGWTFHNHNFIGEKMIPNIFRKMKLPMNEKMK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCHHHHHHHHHHHHCCCHHHHHH
YVQKMVSLHMRPIVIADDVVTDSAVRRLLFEAGDDIDDLMTLCEADITSKNMERKQRFLN
HHHHHHHHCCCCEEEECHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
NFQLVRQKLKDLEEKDRVRNFQPPVSGEEIMETFGLQPCREVGALKSAIKDAILDGVIPN
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCC
EYEAAHAFMLERAAKMGLKPVK
CHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIMDRPTGVFINYLTVCCQSLSIKKMIELTQEELKQYFSEPIFGHIAETADALGLECYVV
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEE
GGYVRDIFLQRPSKDIDVVVVGSGIAMAEALGKRLGRGAYVSVFKNFGTAQVKYKGTEVE
CHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHCCCCEEEEECCCEEE
FVGARKESYQRDSRKPIVEDGTLEDDQNRRDFTINAMAVCLNKARFGELVDPFGGMADMK
EECCCHHHHHHHCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCHHHHH
EKTIRTPLDPDITFSDDPLRMMRCIRFATQLNFYIDDDTFESLCRNKDRIEIISRERIAD
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCEEECCHHHHHHHCCCCHHHHHHHHHHHH
ELNKIILSPIPSKGFVELERSGLLPLIFPELAALQGVETRNGRSHKDNFYHTLEVLDNIS
HHHHHHHCCCCCCCCCHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
KKTDNLWLRWAALLHDIAKPATKRWEPKAGWTFHNHNFIGEKMIPNIFRKMKLPMNEKMK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCHHHHHHHHHHHHCCCHHHHHH
YVQKMVSLHMRPIVIADDVVTDSAVRRLLFEAGDDIDDLMTLCEADITSKNMERKQRFLN
HHHHHHHHCCCCEEEECHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
NFQLVRQKLKDLEEKDRVRNFQPPVSGEEIMETFGLQPCREVGALKSAIKDAILDGVIPN
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCC
EYEAAHAFMLERAAKMGLKPVK
CHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]