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The map label for this gene is leuA [H]

Identifier: 29347271

GI number: 29347271

Start: 2334703

End: 2336199

Strand: Reverse

Name: leuA [H]

Synonym: BT_1861

Alternate gene names: 29347271

Gene position: 2336199-2334703 (Counterclockwise)

Preceding gene: 29347272

Following gene: 29347270

Centisome position: 37.32

GC content: 43.82

Gene sequence:

>1497_bases
ATGGACAATAGGTTATTTATTTTTGATACCACCCTTCGGGATGGTGAACAGGTTCCGGGATGCCAGTTGAACACAGTGGA
GAAGATTCAGGTAGCGAAGGCACTCGAAGCTTTGGGGGTCGATGTAATTGAAGCCGGCTTCCCCATCTCCAGCCCGGGAG
ATTTTAATTCTGTGATTGAAATTTCTAAAGCGGTGACTTGGCCCACTATTTGTGCTTTGACCCGTGCTGTTCAGAAGGAT
ATTGATGTAGCGGTAGACGCTTTGAAATTCGCCAAGCATAAACGTATCCATACAGGTATCGGTACTTCTGATTCTCATAT
TAAATATAAATTCAACTCCAATCGGGAAGAGATTATCGAACGCGCGGTAGCAGCAGTGAAGTATGCACGTCGTTTTGTGG
ATGATGTGGAATTTTATGCAGAGGATGCAGGACGTACCGACAATGAATATCTGGCACGTGTGGTAGAAGCGGTTATCAAA
GCGGGAGCCACAGTGGTGAATATACCGGATACAACGGGTTACTGTCTGCCTTCCGAATATGGTGCAAAGATTAAATATTT
GATTGATCATGTAGACGGCATTGATAACGCGATTCTTTCTACTCACTGTCATAACGATTTGGGAATGGCAACAGCAAATA
CGATTGCTGGTGTTCTGAATGGAGCCCGTCAGGTAGAGGTGACCATTAACGGTATCGGCGAACGTGCTGGAAATACCGCT
CTCGAAGAAATCGCAATGATTATCAAGAGTCATCATGAGATCGATATTCAGACCAATATCAATACACAGAAGATTTATCC
GACCAGCCGTATGGTATCCAGTTTGATGAATATGCCTGTCCAGCCGAATAAAGCAATCGTAGGTCGCAATGCTTTTGCCC
ATTCATCGGGTATCCATCAGGATGGTGTGTTGAAGAATGTAGAAACTTACGAGATTATCGATCCGCATGATGTAGGCATC
GATGATAACTCTATCGTACTGACTGCCCGTAGCGGACGTGCGGCATTGAAGAATCGCCTGTCGTTGTTGGGAGTTAACCT
GGATCAGGAAAAACTGGATAAGGTATATGAGGAGTTTCTGAAACTGGCCGATAAGAAAAAAGATATCAATGATGATGATG
TATTGGTATTGGCAGGAGCAGACCGCAGCCAGAATCATCGTATTAAGTTGGAGTATTTGCAAGTGACAAGTGGTGTCGGT
GTACGTTCGGTTGCTAGTCTGGGACTGAATATTTCCGGTGAGAAGTTTGAAGCTTGTGCGAGTGGTAATGGTCCGGTGGA
TGCTGCCATCAAGGCTCTGAAAAAGATCGTAGATCGTCACATGACATTGAAAGAATTCACTATTCAGGCTATTAGCAAGG
GAAGTGATGATGTAGGTAAGGTTCATATGCAGGTGGAATATGATAATCAGATTTATTATGGCTTCGGAGCAAATACGGAT
ATTATAGCCGCATCGGTAGAAGCTTATATTGACTGTATTAATAAATTCAAGGCATAA

Upstream 100 bases:

>100_bases
CTGCGCGTAAATGTGTATACTGAAAATATACGAAAGCCTTTCTCACTTCCAAGTGTGAAAGGTTTTTTTTTGAGCCAATA
ATAACATAAAATGATAGAAG

Downstream 100 bases:

>100_bases
AGGAACAGCGAAAGTGGTATTGAAATACGTACCCTGCATTAATTATAAATCATACATAGTAAAAATAGATATAACAGATG
AATACATTATTTGATAAAAT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MDNRLFIFDTTLRDGEQVPGCQLNTVEKIQVAKALEALGVDVIEAGFPISSPGDFNSVIEISKAVTWPTICALTRAVQKD
IDVAVDALKFAKHKRIHTGIGTSDSHIKYKFNSNREEIIERAVAAVKYARRFVDDVEFYAEDAGRTDNEYLARVVEAVIK
AGATVVNIPDTTGYCLPSEYGAKIKYLIDHVDGIDNAILSTHCHNDLGMATANTIAGVLNGARQVEVTINGIGERAGNTA
LEEIAMIIKSHHEIDIQTNINTQKIYPTSRMVSSLMNMPVQPNKAIVGRNAFAHSSGIHQDGVLKNVETYEIIDPHDVGI
DDNSIVLTARSGRAALKNRLSLLGVNLDQEKLDKVYEEFLKLADKKKDINDDDVLVLAGADRSQNHRIKLEYLQVTSGVG
VRSVASLGLNISGEKFEACASGNGPVDAAIKALKKIVDRHMTLKEFTIQAISKGSDDVGKVHMQVEYDNQIYYGFGANTD
IIAASVEAYIDCINKFKA

Sequences:

>Translated_498_residues
MDNRLFIFDTTLRDGEQVPGCQLNTVEKIQVAKALEALGVDVIEAGFPISSPGDFNSVIEISKAVTWPTICALTRAVQKD
IDVAVDALKFAKHKRIHTGIGTSDSHIKYKFNSNREEIIERAVAAVKYARRFVDDVEFYAEDAGRTDNEYLARVVEAVIK
AGATVVNIPDTTGYCLPSEYGAKIKYLIDHVDGIDNAILSTHCHNDLGMATANTIAGVLNGARQVEVTINGIGERAGNTA
LEEIAMIIKSHHEIDIQTNINTQKIYPTSRMVSSLMNMPVQPNKAIVGRNAFAHSSGIHQDGVLKNVETYEIIDPHDVGI
DDNSIVLTARSGRAALKNRLSLLGVNLDQEKLDKVYEEFLKLADKKKDINDDDVLVLAGADRSQNHRIKLEYLQVTSGVG
VRSVASLGLNISGEKFEACASGNGPVDAAIKALKKIVDRHMTLKEFTIQAISKGSDDVGKVHMQVEYDNQIYYGFGANTD
IIAASVEAYIDCINKFKA
>Mature_498_residues
MDNRLFIFDTTLRDGEQVPGCQLNTVEKIQVAKALEALGVDVIEAGFPISSPGDFNSVIEISKAVTWPTICALTRAVQKD
IDVAVDALKFAKHKRIHTGIGTSDSHIKYKFNSNREEIIERAVAAVKYARRFVDDVEFYAEDAGRTDNEYLARVVEAVIK
AGATVVNIPDTTGYCLPSEYGAKIKYLIDHVDGIDNAILSTHCHNDLGMATANTIAGVLNGARQVEVTINGIGERAGNTA
LEEIAMIIKSHHEIDIQTNINTQKIYPTSRMVSSLMNMPVQPNKAIVGRNAFAHSSGIHQDGVLKNVETYEIIDPHDVGI
DDNSIVLTARSGRAALKNRLSLLGVNLDQEKLDKVYEEFLKLADKKKDINDDDVLVLAGADRSQNHRIKLEYLQVTSGVG
VRSVASLGLNISGEKFEACASGNGPVDAAIKALKKIVDRHMTLKEFTIQAISKGSDDVGKVHMQVEYDNQIYYGFGANTD
IIAASVEAYIDCINKFKA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=497, Percent_Identity=48.8933601609658, Blast_Score=464, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6320019, Length=374, Percent_Identity=30.4812834224599, Blast_Score=150, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6320071, Length=370, Percent_Identity=30.5405405405405, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6324682, Length=551, Percent_Identity=25.5898366606171, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6324225, Length=542, Percent_Identity=26.9372693726937, Blast_Score=146, Evalue=6e-36,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 54416; Mature: 54416

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS01181 RIBOSOMAL_S21 ; PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNRLFIFDTTLRDGEQVPGCQLNTVEKIQVAKALEALGVDVIEAGFPISSPGDFNSVIE
CCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCHHHHH
ISKAVTWPTICALTRAVQKDIDVAVDALKFAKHKRIHTGIGTSDSHIKYKFNSNREEIIE
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHH
RAVAAVKYARRFVDDVEFYAEDAGRTDNEYLARVVEAVIKAGATVVNIPDTTGYCLPSEY
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHH
GAKIKYLIDHVDGIDNAILSTHCHNDLGMATANTIAGVLNGARQVEVTINGIGERAGNTA
CCHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCHH
LEEIAMIIKSHHEIDIQTNINTQKIYPTSRMVSSLMNMPVQPNKAIVGRNAFAHSSGIHQ
HHHHHHHHHCCCCEEEEECCCCEEECCHHHHHHHHHCCCCCCCCEEECCCCHHCCCCCCC
DGVLKNVETYEIIDPHDVGIDDNSIVLTARSGRAALKNRLSLLGVNLDQEKLDKVYEEFL
CCCCCCCCEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHEECCCCHHHHHHHHHHHH
KLADKKKDINDDDVLVLAGADRSQNHRIKLEYLQVTSGVGVRSVASLGLNISGEKFEACA
HHHHHCCCCCCCCEEEEECCCCCCCCEEEEEEEEEECCCCHHHHHHHCCCCCCCHHHHHC
SGNGPVDAAIKALKKIVDRHMTLKEFTIQAISKGSDDVGKVHMQVEYDNQIYYGFGANTD
CCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEEEEECCEEEEEECCCCC
IIAASVEAYIDCINKFKA
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDNRLFIFDTTLRDGEQVPGCQLNTVEKIQVAKALEALGVDVIEAGFPISSPGDFNSVIE
CCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCHHHHH
ISKAVTWPTICALTRAVQKDIDVAVDALKFAKHKRIHTGIGTSDSHIKYKFNSNREEIIE
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHH
RAVAAVKYARRFVDDVEFYAEDAGRTDNEYLARVVEAVIKAGATVVNIPDTTGYCLPSEY
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHH
GAKIKYLIDHVDGIDNAILSTHCHNDLGMATANTIAGVLNGARQVEVTINGIGERAGNTA
CCHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCHH
LEEIAMIIKSHHEIDIQTNINTQKIYPTSRMVSSLMNMPVQPNKAIVGRNAFAHSSGIHQ
HHHHHHHHHCCCCEEEEECCCCEEECCHHHHHHHHHCCCCCCCCEEECCCCHHCCCCCCC
DGVLKNVETYEIIDPHDVGIDDNSIVLTARSGRAALKNRLSLLGVNLDQEKLDKVYEEFL
CCCCCCCCEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHEECCCCHHHHHHHHHHHH
KLADKKKDINDDDVLVLAGADRSQNHRIKLEYLQVTSGVGVRSVASLGLNISGEKFEACA
HHHHHCCCCCCCCEEEEECCCCCCCCEEEEEEEEEECCCCHHHHHHHCCCCCCCHHHHHC
SGNGPVDAAIKALKKIVDRHMTLKEFTIQAISKGSDDVGKVHMQVEYDNQIYYGFGANTD
CCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEEEEECCEEEEEECCCCC
IIAASVEAYIDCINKFKA
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA