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The map label for this gene is leuC [H]

Identifier: 29347270

GI number: 29347270

Start: 2333231

End: 2334625

Strand: Reverse

Name: leuC [H]

Synonym: BT_1860

Alternate gene names: 29347270

Gene position: 2334625-2333231 (Counterclockwise)

Preceding gene: 29347271

Following gene: 29347269

Centisome position: 37.29

GC content: 48.32

Gene sequence:

>1395_bases
ATGAATACATTATTTGATAAAATATGGGATGCCCACGTGGTGACTACGGTGGAAGATGGGCCGACACAACTTTATATTGA
TCGTTTATATTGTCATGAAGTGACCAGTCCGCAGGCTTTTGCCGGATTGCGCGAGCGTGGCATCAAGGTATTGCGTCCGG
AAAAGGTATTCTGTATGCCCGATCACAATACTCCGACACATGATCAGGATAAACCGATTGAAGACCCGATCTCCAAGACT
CAGGTGGATACACTGACGAAAAATGCGAAAGATTTCGGTCTGACACATTTCGGTATGATGCATCCTAAAAACGGAATTAT
CCATGTGGTAGGTCCGGAACGTGCACTGACACTGCCGGGCATGACGATTGTTTGCGGTGACTCGCATACTTCTACTCACG
GTGCAATGGGAGCTATCGCTTTCGGCATCGGTACCAGTGAAGTGGAAATGGTACTGGCTTCACAATGTATCCTTCAGTCA
AGACCGAAAACAATGCGAATTACCGTAGATGGCGAACTGGGTAAAGGAGTGACTGCGAAAGATGTCGCTCTGTACATGAT
GTCTAAGATGACTACCAGCGGAGCTACCGGTTACTTCGTGGAATATGCAGGTTCGGCCATCCGCAATCTGACTATGGAAG
GACGTCTTACTCTTTGTAATCTGTCCATTGAAATGGGAGCACGTGGTGGTATGGTTGCTCCGGATGAAGTGACTTTCGAA
TATATCAAAGGACGTGAGAATGCTCCGCAGGGGGAAGCATGGGATCAGGCTATGGAATACTGGAAAACGCTGAAGAGCGA
TGATGACGCCGTATTCGATCAGGAGGTCCGTTTTGATGCAGCGGATATCGAGCCGATGATTACGTACGGAACCAATCCGG
GCATGGGAATGGGAATTACGCAAAACATTCCTACTACGGAAGGAATGGGAGAGGCTGCACAGGTTTCTTTCAAAAAATCG
ATGGAATATATGGGATTCCAGCCGGGTGAATCGTTACTGGGTAAAAAGATTGATTACGTATTTCTGGGTGCTTGTACTAA
TGGCCGTATTGAGGACTTCCGCGCATTCGCTTCTCTAGTGAAGGGACGCAGGAAAGCGGATAATGTAATCGCATGGCTGG
TTCCGGGATCATGGATGGTTGATGCACAGATTCGTAAGGAAGGAATTGACAAGATATTGACTGAAGCGGGATTTGCAATC
CGTCAGCCGGGATGTTCAGCATGCCTTGCGATGAATGATGACAAGATTCCTGCAGGAAAATATTCAGTATCAACCAGTAA
CCGTAACTTTGAAGGTCGTCAGGGACCGGGAGCCCGTACTTTACTGGCCAGCCCGTTGGTTGCTGCTGCCGCTGCGGTGA
CAGGAGTGATTACCGACCCGCGTGAGCTGATGTAA

Upstream 100 bases:

>100_bases
GTATTAATAAATTCAAGGCATAAAGGAACAGCGAAAGTGGTATTGAAATACGTACCCTGCATTAATTATAAATCATACAT
AGTAAAAATAGATATAACAG

Downstream 100 bases:

>100_bases
TTTCAATCGTAATTTGTAAATATTTAAATTGTAAACAGAAAGATGGCTAAGACAAAATTTAATATAATAACAAGTACTTG
TGTACCCCTTCCTTTAGAGA

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MNTLFDKIWDAHVVTTVEDGPTQLYIDRLYCHEVTSPQAFAGLRERGIKVLRPEKVFCMPDHNTPTHDQDKPIEDPISKT
QVDTLTKNAKDFGLTHFGMMHPKNGIIHVVGPERALTLPGMTIVCGDSHTSTHGAMGAIAFGIGTSEVEMVLASQCILQS
RPKTMRITVDGELGKGVTAKDVALYMMSKMTTSGATGYFVEYAGSAIRNLTMEGRLTLCNLSIEMGARGGMVAPDEVTFE
YIKGRENAPQGEAWDQAMEYWKTLKSDDDAVFDQEVRFDAADIEPMITYGTNPGMGMGITQNIPTTEGMGEAAQVSFKKS
MEYMGFQPGESLLGKKIDYVFLGACTNGRIEDFRAFASLVKGRRKADNVIAWLVPGSWMVDAQIRKEGIDKILTEAGFAI
RQPGCSACLAMNDDKIPAGKYSVSTSNRNFEGRQGPGARTLLASPLVAAAAAVTGVITDPRELM

Sequences:

>Translated_464_residues
MNTLFDKIWDAHVVTTVEDGPTQLYIDRLYCHEVTSPQAFAGLRERGIKVLRPEKVFCMPDHNTPTHDQDKPIEDPISKT
QVDTLTKNAKDFGLTHFGMMHPKNGIIHVVGPERALTLPGMTIVCGDSHTSTHGAMGAIAFGIGTSEVEMVLASQCILQS
RPKTMRITVDGELGKGVTAKDVALYMMSKMTTSGATGYFVEYAGSAIRNLTMEGRLTLCNLSIEMGARGGMVAPDEVTFE
YIKGRENAPQGEAWDQAMEYWKTLKSDDDAVFDQEVRFDAADIEPMITYGTNPGMGMGITQNIPTTEGMGEAAQVSFKKS
MEYMGFQPGESLLGKKIDYVFLGACTNGRIEDFRAFASLVKGRRKADNVIAWLVPGSWMVDAQIRKEGIDKILTEAGFAI
RQPGCSACLAMNDDKIPAGKYSVSTSNRNFEGRQGPGARTLLASPLVAAAAAVTGVITDPRELM
>Mature_464_residues
MNTLFDKIWDAHVVTTVEDGPTQLYIDRLYCHEVTSPQAFAGLRERGIKVLRPEKVFCMPDHNTPTHDQDKPIEDPISKT
QVDTLTKNAKDFGLTHFGMMHPKNGIIHVVGPERALTLPGMTIVCGDSHTSTHGAMGAIAFGIGTSEVEMVLASQCILQS
RPKTMRITVDGELGKGVTAKDVALYMMSKMTTSGATGYFVEYAGSAIRNLTMEGRLTLCNLSIEMGARGGMVAPDEVTFE
YIKGRENAPQGEAWDQAMEYWKTLKSDDDAVFDQEVRFDAADIEPMITYGTNPGMGMGITQNIPTTEGMGEAAQVSFKKS
MEYMGFQPGESLLGKKIDYVFLGACTNGRIEDFRAFASLVKGRRKADNVIAWLVPGSWMVDAQIRKEGIDKILTEAGFAI
RQPGCSACLAMNDDKIPAGKYSVSTSNRNFEGRQGPGARTLLASPLVAAAAAVTGVITDPRELM

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=477, Percent_Identity=25.3668763102725, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI8659555, Length=440, Percent_Identity=23.8636363636364, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI41352693, Length=404, Percent_Identity=25, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1786259, Length=462, Percent_Identity=53.6796536796537, Blast_Score=495, Evalue=1e-141,
Organism=Escherichia coli, GI1787531, Length=402, Percent_Identity=26.1194029850746, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI2367097, Length=401, Percent_Identity=26.9326683291771, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI87081781, Length=364, Percent_Identity=25, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI25149337, Length=471, Percent_Identity=26.3269639065817, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI32564738, Length=366, Percent_Identity=28.1420765027322, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI25149342, Length=302, Percent_Identity=27.1523178807947, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17568399, Length=437, Percent_Identity=24.0274599542334, Blast_Score=91, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6321429, Length=469, Percent_Identity=50.7462686567164, Blast_Score=470, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6322261, Length=447, Percent_Identity=26.8456375838926, Blast_Score=140, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6323335, Length=446, Percent_Identity=28.0269058295964, Blast_Score=136, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6320440, Length=450, Percent_Identity=24.6666666666667, Blast_Score=114, Evalue=4e-26,
Organism=Drosophila melanogaster, GI281365315, Length=445, Percent_Identity=25.3932584269663, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI17864292, Length=445, Percent_Identity=25.3932584269663, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI161076999, Length=418, Percent_Identity=25.5980861244019, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI28571643, Length=471, Percent_Identity=25.2653927813163, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI17137564, Length=443, Percent_Identity=23.9277652370203, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24645686, Length=468, Percent_Identity=24.3589743589744, Blast_Score=88, Evalue=2e-17,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50491; Mature: 50491

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTLFDKIWDAHVVTTVEDGPTQLYIDRLYCHEVTSPQAFAGLRERGIKVLRPEKVFCMP
CCCHHHHHCCCEEEEEECCCCCEEEEEEEEHHCCCCCHHHHHHHHCCCEEECCCEEEEEC
DHNTPTHDQDKPIEDPISKTQVDTLTKNAKDFGLTHFGMMHPKNGIIHVVGPERALTLPG
CCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHCCCCCEECCCCCCCEEEEECCCCEEECCC
MTIVCGDSHTSTHGAMGAIAFGIGTSEVEMVLASQCILQSRPKTMRITVDGELGKGVTAK
EEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHH
DVALYMMSKMTTSGATGYFVEYAGSAIRNLTMEGRLTLCNLSIEMGARGGMVAPDEVTFE
HHHHHHHHHHHCCCCCEEHHHHHHHHHHHEEECCEEEEEEEEEEECCCCCCCCCHHHHHH
YIKGRENAPQGEAWDQAMEYWKTLKSDDDAVFDQEVRFDAADIEPMITYGTNPGMGMGIT
HHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCEEEEECCCCCCCCCCC
QNIPTTEGMGEAAQVSFKKSMEYMGFQPGESLLGKKIDYVFLGACTNGRIEDFRAFASLV
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHCCCCCEEEEEECCCCCHHHHHHHHHHH
KGRRKADNVIAWLVPGSWMVDAQIRKEGIDKILTEAGFAIRQPGCSACLAMNDDKIPAGK
HCCCCCCCEEEEEECCCEEEEHHHHHHHHHHHHHHCCCEECCCCCCEEEEECCCCCCCCC
YSVSTSNRNFEGRQGPGARTLLASPLVAAAAAVTGVITDPRELM
EEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCC
>Mature Secondary Structure
MNTLFDKIWDAHVVTTVEDGPTQLYIDRLYCHEVTSPQAFAGLRERGIKVLRPEKVFCMP
CCCHHHHHCCCEEEEEECCCCCEEEEEEEEHHCCCCCHHHHHHHHCCCEEECCCEEEEEC
DHNTPTHDQDKPIEDPISKTQVDTLTKNAKDFGLTHFGMMHPKNGIIHVVGPERALTLPG
CCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHCCCCCEECCCCCCCEEEEECCCCEEECCC
MTIVCGDSHTSTHGAMGAIAFGIGTSEVEMVLASQCILQSRPKTMRITVDGELGKGVTAK
EEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHH
DVALYMMSKMTTSGATGYFVEYAGSAIRNLTMEGRLTLCNLSIEMGARGGMVAPDEVTFE
HHHHHHHHHHHCCCCCEEHHHHHHHHHHHEEECCEEEEEEEEEEECCCCCCCCCHHHHHH
YIKGRENAPQGEAWDQAMEYWKTLKSDDDAVFDQEVRFDAADIEPMITYGTNPGMGMGIT
HHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCEEEEECCCCCCCCCCC
QNIPTTEGMGEAAQVSFKKSMEYMGFQPGESLLGKKIDYVFLGACTNGRIEDFRAFASLV
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHCCCCCEEEEEECCCCCHHHHHHHHHHH
KGRRKADNVIAWLVPGSWMVDAQIRKEGIDKILTEAGFAIRQPGCSACLAMNDDKIPAGK
HCCCCCCCEEEEEECCCEEEEHHHHHHHHHHHHHHCCCEECCCCCCEEEEECCCCCCCCC
YSVSTSNRNFEGRQGPGARTLLASPLVAAAAAVTGVITDPRELM
EEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA