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The map label for this gene is hisA

Identifier: 29346789

GI number: 29346789

Start: 1707031

End: 1707750

Strand: Reverse

Name: hisA

Synonym: BT_1379

Alternate gene names: 29346789

Gene position: 1707750-1707031 (Counterclockwise)

Preceding gene: 29346790

Following gene: 29346788

Centisome position: 27.28

GC content: 44.58

Gene sequence:

>720_bases
ATGATAGAAATTATTCCAGCCATTGATATTATTGACGGGAAATGTGTACGCCTTTCTCAGGGAGATTACGATAGTAAGAA
GGTCTATAACGAAAATCCGGTGGAAGTAGCCAAGGAGTTCGAAGCGAACGGGGTTCGCAGGCTTCATGTGGTAGATCTGG
ACGGAGCCGCTTCTCACCACGTAGTCAACCATCGGGTATTGGAGCAGATTGCTACGCGTACCTCTTTAATAGTGGATTTC
GGCGGTGGAGTAAAGAGTGACGAAGATTTGAAAATAGCTTTCGAAAGCGGTGCTCAGATGGTGACAGGCGGAAGCGTTGC
CGTGAAAGATCCGGAGCTGTTCTGTCATTGGCTGGAAGTATATGGCAGTGAGAAAATAATCCTCGGAGCAGACGTCAAGG
AACATAAGATTGCCGTAAACGGCTGGAAAGACGAAAGCGCCTGCGAGCTTTTCCCATTTCTCGAAGACTATATAAATAAA
GGTATTCAGAAAGTCATTTGCACTGATATTAGTTGTGATGGAATGCTGAAAGGTCCTTCTATCGATTTGTATAAAGAAAT
GCTGGAGAAGTTTCCGAATCTCTATCTGATGGCTAGTGGCGGAGTGAGCAACGTGGATGACATTATCGCCTTGAATGAAG
CCGGAGTTCCCGGAGTCATCTTCGGTAAAGCATTGTACGAAGGGCATATAACATTGAAAGATTTGCGTATATTTTTATAA

Upstream 100 bases:

>100_bases
TATTTCCGGCTTTTCGGAGAAGAAGAAAAGGACAGGTATAAGAAGATGGAGTGAACAGCTAAAATAGTAAATCGTAAATG
ATCAAATTGTATATAAACAG

Downstream 100 bases:

>100_bases
TTAGGAGTGCTAAAATGAAATGGAGCGCCCTGTTATAATGAAAAAATAAAGAAGCAGGTTCTCGGATTAGTAGAATCTAA
ATTGTCAAATAGTAAATAAG

Product: phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MIEIIPAIDIIDGKCVRLSQGDYDSKKVYNENPVEVAKEFEANGVRRLHVVDLDGAASHHVVNHRVLEQIATRTSLIVDF
GGGVKSDEDLKIAFESGAQMVTGGSVAVKDPELFCHWLEVYGSEKIILGADVKEHKIAVNGWKDESACELFPFLEDYINK
GIQKVICTDISCDGMLKGPSIDLYKEMLEKFPNLYLMASGGVSNVDDIIALNEAGVPGVIFGKALYEGHITLKDLRIFL

Sequences:

>Translated_239_residues
MIEIIPAIDIIDGKCVRLSQGDYDSKKVYNENPVEVAKEFEANGVRRLHVVDLDGAASHHVVNHRVLEQIATRTSLIVDF
GGGVKSDEDLKIAFESGAQMVTGGSVAVKDPELFCHWLEVYGSEKIILGADVKEHKIAVNGWKDESACELFPFLEDYINK
GIQKVICTDISCDGMLKGPSIDLYKEMLEKFPNLYLMASGGVSNVDDIIALNEAGVPGVIFGKALYEGHITLKDLRIFL
>Mature_239_residues
MIEIIPAIDIIDGKCVRLSQGDYDSKKVYNENPVEVAKEFEANGVRRLHVVDLDGAASHHVVNHRVLEQIATRTSLIVDF
GGGVKSDEDLKIAFESGAQMVTGGSVAVKDPELFCHWLEVYGSEKIILGADVKEHKIAVNGWKDESACELFPFLEDYINK
GIQKVICTDISCDGMLKGPSIDLYKEMLEKFPNLYLMASGGVSNVDDIIALNEAGVPGVIFGKALYEGHITLKDLRIFL

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=236, Percent_Identity=38.135593220339, Blast_Score=182, Evalue=1e-47,
Organism=Escherichia coli, GI1788336, Length=248, Percent_Identity=23.7903225806452, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_BACTN (Q8A7Z5)

Other databases:

- EMBL:   AE015928
- RefSeq:   NP_810292.1
- ProteinModelPortal:   Q8A7Z5
- SMR:   Q8A7Z5
- GeneID:   1072956
- GenomeReviews:   AE015928_GR
- KEGG:   bth:BT_1379
- NMPDR:   fig|226186.1.peg.1379
- HOGENOM:   HBG541613
- OMA:   LLAINGW
- PhylomeDB:   Q8A7Z5
- ProtClustDB:   CLSK822682
- BioCyc:   BTHE226186:BT_1379-MONOMER
- BRENDA:   5.3.1.16
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 26268; Mature: 26268

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: ACT_SITE 9-9 ACT_SITE 131-131

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEIIPAIDIIDGKCVRLSQGDYDSKKVYNENPVEVAKEFEANGVRRLHVVDLDGAASHH
CEEEEEEEEEECCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEEECCCCCCHH
VVNHRVLEQIATRTSLIVDFGGGVKSDEDLKIAFESGAQMVTGGSVAVKDPELFCHWLEV
HHHHHHHHHHHHHHEEEEECCCCCCCCCCEEEEECCCCEEEECCEEEEECHHHHHHHHHH
YGSEKIILGADVKEHKIAVNGWKDESACELFPFLEDYINKGIQKVICTDISCDGMLKGPS
HCCCEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHEEHHCCCCCCCEECCCC
IDLYKEMLEKFPNLYLMASGGVSNVDDIIALNEAGVPGVIFGKALYEGHITLKDLRIFL
HHHHHHHHHHCCCEEEEECCCCCCCCCEEEECCCCCCEEEEHHHHHCCCEEEEEEEEEC
>Mature Secondary Structure
MIEIIPAIDIIDGKCVRLSQGDYDSKKVYNENPVEVAKEFEANGVRRLHVVDLDGAASHH
CEEEEEEEEEECCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEEECCCCCCHH
VVNHRVLEQIATRTSLIVDFGGGVKSDEDLKIAFESGAQMVTGGSVAVKDPELFCHWLEV
HHHHHHHHHHHHHHEEEEECCCCCCCCCCEEEEECCCCEEEECCEEEEECHHHHHHHHHH
YGSEKIILGADVKEHKIAVNGWKDESACELFPFLEDYINKGIQKVICTDISCDGMLKGPS
HCCCEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHEEHHCCCCCCCEECCCC
IDLYKEMLEKFPNLYLMASGGVSNVDDIIALNEAGVPGVIFGKALYEGHITLKDLRIFL
HHHHHHHHHHCCCEEEEECCCCCCCCCEEEECCCCCCEEEEHHHHHCCCEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928