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The map label for this gene is hisH

Identifier: 29346790

GI number: 29346790

Start: 1707922

End: 1708512

Strand: Reverse

Name: hisH

Synonym: BT_1380

Alternate gene names: 29346790

Gene position: 1708512-1707922 (Counterclockwise)

Preceding gene: 29346791

Following gene: 29346789

Centisome position: 27.29

GC content: 45.85

Gene sequence:

>591_bases
ATGAAAGTAGCAGTTGTAAAATACAATGCCGGGAATATCCGTTCTGTGGACTATGCTCTGAAGCGGTTGGGAGTAGAAGC
AGTGATTACTGCCGATAAAGAAATACTTCAATCTGCCGATAAAGTGATTTTCCCCGGAGTAGGAGAGGCGGGAACTACCA
TGAATCACCTGAAAGCAACAGGGCTCGACGAGCTGATCAAGAATCTGCGTCAGCCTGTGTTCGGAATTTGCCTCGGTATG
CAGCTGATGTGCCGACATTCTGAAGAAGGTGAGGTGGACTGCCTGAATATCTTTGATGTAGACGTGAAACGTTTTGTTCC
GCAGAAGCATGAAGATAAAGTGCCGCATATGGGCTGGAATACGATTGGTAAGACAAACAGCAAACTGTTCGAAGGATTCA
CCGAAGAAGAGTTTGTCTACTTTGTACACAGTTTCTATGTGCCGGTTTGTGACTTTACTGCTGCTGAAACCGATTATATC
CATCCGTTCAGCGCGGCATTGCATAAAGATAACTTTTATGCCACCCAGTTCCATCCGGAGAAAAGCGGGAAGACAGGTGA
AAGGGTTCTGAGGAACTTTCTGGATCTCTAA

Upstream 100 bases:

>100_bases
AAGGAAAGGACCTGGAAAAAATTGTTCTTTCGCGTGCGGTACAAAAGCATATCGAACGTAAGATTTTGGCTTATAAAAAT
AAAACAGTAATATTTAGCTG

Downstream 100 bases:

>100_bases
TAAACGTTGCAACAGTTTATAATAAACTCCGCTATAGTTTATAATAAACTCGGTCATCGTTTATTATAAACTATTTCCGG
CTTTTCGGAGAAGAAGAAAA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MKVAVVKYNAGNIRSVDYALKRLGVEAVITADKEILQSADKVIFPGVGEAGTTMNHLKATGLDELIKNLRQPVFGICLGM
QLMCRHSEEGEVDCLNIFDVDVKRFVPQKHEDKVPHMGWNTIGKTNSKLFEGFTEEEFVYFVHSFYVPVCDFTAAETDYI
HPFSAALHKDNFYATQFHPEKSGKTGERVLRNFLDL

Sequences:

>Translated_196_residues
MKVAVVKYNAGNIRSVDYALKRLGVEAVITADKEILQSADKVIFPGVGEAGTTMNHLKATGLDELIKNLRQPVFGICLGM
QLMCRHSEEGEVDCLNIFDVDVKRFVPQKHEDKVPHMGWNTIGKTNSKLFEGFTEEEFVYFVHSFYVPVCDFTAAETDYI
HPFSAALHKDNFYATQFHPEKSGKTGERVLRNFLDL
>Mature_196_residues
MKVAVVKYNAGNIRSVDYALKRLGVEAVITADKEILQSADKVIFPGVGEAGTTMNHLKATGLDELIKNLRQPVFGICLGM
QLMCRHSEEGEVDCLNIFDVDVKRFVPQKHEDKVPHMGWNTIGKTNSKLFEGFTEEEFVYFVHSFYVPVCDFTAAETDYI
HPFSAALHKDNFYATQFHPEKSGKTGERVLRNFLDL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=198, Percent_Identity=42.9292929292929, Blast_Score=171, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6319725, Length=209, Percent_Identity=33.4928229665072, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_BACTN (Q8A7Z4)

Other databases:

- EMBL:   AE015928
- RefSeq:   NP_810293.1
- ProteinModelPortal:   Q8A7Z4
- SMR:   Q8A7Z4
- GeneID:   1072955
- GenomeReviews:   AE015928_GR
- KEGG:   bth:BT_1380
- NMPDR:   fig|226186.1.peg.1380
- HOGENOM:   HBG292341
- OMA:   SVRFAFE
- PhylomeDB:   Q8A7Z4
- ProtClustDB:   PRK13181
- BioCyc:   BTHE226186:BT_1380-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 22135; Mature: 22135

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 77-77 ACT_SITE 178-178 ACT_SITE 180-180

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAVVKYNAGNIRSVDYALKRLGVEAVITADKEILQSADKVIFPGVGEAGTTMNHLKAT
CEEEEEEECCCCCHHHHHHHHHCCHHHEEECCHHHHHCCCCEECCCCCCCCCHHHHHHHC
GLDELIKNLRQPVFGICLGMQLMCRHSEEGEVDCLNIFDVDVKRFVPQKHEDKVPHMGWN
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECHHHHHCCCCCCCCCCCCCCC
TIGKTNSKLFEGFTEEEFVYFVHSFYVPVCDFTAAETDYIHPFSAALHKDNFYATQFHPE
CCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCEEEEEECCC
KSGKTGERVLRNFLDL
CCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKVAVVKYNAGNIRSVDYALKRLGVEAVITADKEILQSADKVIFPGVGEAGTTMNHLKAT
CEEEEEEECCCCCHHHHHHHHHCCHHHEEECCHHHHHCCCCEECCCCCCCCCHHHHHHHC
GLDELIKNLRQPVFGICLGMQLMCRHSEEGEVDCLNIFDVDVKRFVPQKHEDKVPHMGWN
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECHHHHHCCCCCCCCCCCCCCC
TIGKTNSKLFEGFTEEEFVYFVHSFYVPVCDFTAAETDYIHPFSAALHKDNFYATQFHPE
CCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCEEEEEECCC
KSGKTGERVLRNFLDL
CCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928