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The map label for this gene is rfbF [H]

Identifier: 29346761

GI number: 29346761

Start: 1676081

End: 1676854

Strand: Reverse

Name: rfbF [H]

Synonym: BT_1351

Alternate gene names: 29346761

Gene position: 1676854-1676081 (Counterclockwise)

Preceding gene: 29346762

Following gene: 29346760

Centisome position: 26.79

GC content: 35.4

Gene sequence:

>774_bases
ATGAAAGCTGTGATATTAGCAGGCGGGTATGGTACTCGGTTAAGTGAGGCAACTAATCTGATTCCTAAGCCAATGGTGGA
AATTGGAGGAAAACCCATTCTATGGCATATAATGAAGATATATAGCTATTATGGAATAAATGATTTTATTATTTGCTGTG
GTTATAAACAATATGTTATAAAAGAGTATTTCTCAAATTATTTTCGACATAACTCGGATCTAACTGTGGATTTGTCAACA
AATAGTATACAAATTCATGATAATCATTCTGAACATTGGAAAGTAACGATGGTGGATACTGGTTTGAATACAATGACAGG
TGGTCGCATTAAACGTGTACAGAAATACATTGGTAATGAGGCTTTTTGCCTTACTTATGGTGATGGAGTTGCAGATATTA
ATATTGCAGATACGATTGATCAACATAAAAAATCAGGTAAAGCTCTTTCTATGACAGCTTATCAACCTGGTGGACGATTG
GGAGTGTTGGATATACTGGATGATGGAACTTTAGATTCTTTTGTCGAGAAGCCTCAAGAAAGTGGTACTTGGATTAATGC
AGGTTTTTTCATTTGTGAGCCTTTCATTTTTAATATGTTGAAGGGAGACATGGAAATGTTTGAAAAAGAACCAATGCAAC
GTTTAGTAGCACAGAAACAAGTACATGTGTATAAACACACAGGCTTTTGGAAGCCGATGGATACACTTCGTGATAATGCA
GAACTAAATGCAATGTGGGATAAGAGAAATGCTCCTTGGCAAATTTGGAAATAA

Upstream 100 bases:

>100_bases
ATGGCAAAGGCGGCTATTAAAAATAGTAAGCGTTTTGCAGTGGAGAATATTGTTGCTCAATGGGTAGAATTATTTCAAGA
ACTAACTTTAAATAAATAAA

Downstream 100 bases:

>100_bases
AATATATATATATGGCATTTAATAACGTATATCAAGGCAAGAAAGTATTAGTAACTGGAAATACAGGTTTTAAAGGTTCA
TGGTTATCGACTTGGTTGTT

Product: glucose-1-phosphate cytidylyltransferase

Products: NA

Alternate protein names: CDP-glucose pyrophosphorylase [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MKAVILAGGYGTRLSEATNLIPKPMVEIGGKPILWHIMKIYSYYGINDFIICCGYKQYVIKEYFSNYFRHNSDLTVDLST
NSIQIHDNHSEHWKVTMVDTGLNTMTGGRIKRVQKYIGNEAFCLTYGDGVADINIADTIDQHKKSGKALSMTAYQPGGRL
GVLDILDDGTLDSFVEKPQESGTWINAGFFICEPFIFNMLKGDMEMFEKEPMQRLVAQKQVHVYKHTGFWKPMDTLRDNA
ELNAMWDKRNAPWQIWK

Sequences:

>Translated_257_residues
MKAVILAGGYGTRLSEATNLIPKPMVEIGGKPILWHIMKIYSYYGINDFIICCGYKQYVIKEYFSNYFRHNSDLTVDLST
NSIQIHDNHSEHWKVTMVDTGLNTMTGGRIKRVQKYIGNEAFCLTYGDGVADINIADTIDQHKKSGKALSMTAYQPGGRL
GVLDILDDGTLDSFVEKPQESGTWINAGFFICEPFIFNMLKGDMEMFEKEPMQRLVAQKQVHVYKHTGFWKPMDTLRDNA
ELNAMWDKRNAPWQIWK
>Mature_257_residues
MKAVILAGGYGTRLSEATNLIPKPMVEIGGKPILWHIMKIYSYYGINDFIICCGYKQYVIKEYFSNYFRHNSDLTVDLST
NSIQIHDNHSEHWKVTMVDTGLNTMTGGRIKRVQKYIGNEAFCLTYGDGVADINIADTIDQHKKSGKALSMTAYQPGGRL
GVLDILDDGTLDSFVEKPQESGTWINAGFFICEPFIFNMLKGDMEMFEKEPMQRLVAQKQVHVYKHTGFWKPMDTLRDNA
ELNAMWDKRNAPWQIWK

Specific function: Involved in the biosynthesis of the tyvelose, a 3,6- dideoxyhexose found in the O-antigen of the surface lipopolysaccharides. It catalyzes the transfer of a CMP moiety from CTP to glucose 1-phosphate. This enzyme can utilize either CTP or UTP as the nucle

COG id: COG1208

COG function: function code MJ; Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate cytidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=244, Percent_Identity=25.8196721311475, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI11761619, Length=244, Percent_Identity=25.8196721311475, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI133931050, Length=246, Percent_Identity=28.4552845528455, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320148, Length=238, Percent_Identity=28.1512605042017, Blast_Score=101, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21355443, Length=240, Percent_Identity=25.4166666666667, Blast_Score=84, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24644084, Length=240, Percent_Identity=25.4166666666667, Blast_Score=84, Evalue=6e-17,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013446
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.33 [H]

Molecular weight: Translated: 29410; Mature: 29410

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAVILAGGYGTRLSEATNLIPKPMVEIGGKPILWHIMKIYSYYGINDFIICCGYKQYVI
CCEEEEECCCCCHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHH
KEYFSNYFRHNSDLTVDLSTNSIQIHDNHSEHWKVTMVDTGLNTMTGGRIKRVQKYIGNE
HHHHHHHHHCCCCEEEEEECCEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHCCC
AFCLTYGDGVADINIADTIDQHKKSGKALSMTAYQPGGRLGVLDILDDGTLDSFVEKPQE
EEEEEECCCEEECCHHHHHHHHHHCCCEEEEEEECCCCCEEEEEECCCCCHHHHHHCHHH
SGTWINAGFFICEPFIFNMLKGDMEMFEKEPMQRLVAQKQVHVYKHTGFWKPMDTLRDNA
CCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHCCC
ELNAMWDKRNAPWQIWK
CEEEEECCCCCCEECCC
>Mature Secondary Structure
MKAVILAGGYGTRLSEATNLIPKPMVEIGGKPILWHIMKIYSYYGINDFIICCGYKQYVI
CCEEEEECCCCCHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHH
KEYFSNYFRHNSDLTVDLSTNSIQIHDNHSEHWKVTMVDTGLNTMTGGRIKRVQKYIGNE
HHHHHHHHHCCCCEEEEEECCEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHCCC
AFCLTYGDGVADINIADTIDQHKKSGKALSMTAYQPGGRLGVLDILDDGTLDSFVEKPQE
EEEEEECCCEEECCHHHHHHHHHHCCCEEEEEEECCCCCEEEEEECCCCCHHHHHHCHHH
SGTWINAGFFICEPFIFNMLKGDMEMFEKEPMQRLVAQKQVHVYKHTGFWKPMDTLRDNA
CCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHCCC
ELNAMWDKRNAPWQIWK
CEEEEECCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]