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The map label for this gene is rfbG [H]

Identifier: 29346760

GI number: 29346760

Start: 1674969

End: 1676069

Strand: Reverse

Name: rfbG [H]

Synonym: BT_1350

Alternate gene names: 29346760

Gene position: 1676069-1674969 (Counterclockwise)

Preceding gene: 29346761

Following gene: 29346759

Centisome position: 26.77

GC content: 35.97

Gene sequence:

>1101_bases
ATGGCATTTAATAACGTATATCAAGGCAAGAAAGTATTAGTAACTGGAAATACAGGTTTTAAAGGTTCATGGTTATCGAC
TTGGTTGTTGATGATGGGGGCTGATGTATATGGATATTCAAGTGATATTCCTACTGATCCCTCAATGTTTGAAACACTTA
AACTCAAAGACAAACTTCATCACTGTTTTGGTGATATTCGTGATAAAGCAAAACTGAATGAGTTTGTGCAAAAAGTAAAG
CCCGACTTTATTTTCCATTTAGCTGCGCAAGCTATTGTTTCTACTTCTTATAAATTTCCTTTTGATACAATAACTACAAA
TGTTGTTGGGACGGCATCCGTATTGGAAGCTATTTGTAATATAACATGGCATTGTACGTGTGTATTGATAACAAGTGATA
AAGCGTATGATAATGTGGAATGGATATGGGGATATCGAGAAACAGATGCTATGGGAGGAAAAGATGTATATTCGGGTTCT
AAAGGTGCGGCAGAACTAGTTATAAAATGTTACTGGAAATCTTTTATTCAGGTTATGCCGAATATAAAGTTGGGAGTAGC
ACGTGCAGGGAATGTTATAGGTGGGGGAGATTGGGCTAAAGATAGAATTATAGTAGATTGTATTAATGCTTTTTCTCAGA
ATAGGATTGTTGAGGTGCGTTGTCCTAAAGCTACTCGCCCATGGCAGCATGTACTCGAGCCGTTGAGTGGATATTTGGTT
TTAGGACAGTATCTATTTGAAGATAAAGTAAAGAATGGAGAGCCTTTTAATTTTGGTCCACGTGCAGAGCAAACTAAAAC
TGTATTTGAGTTGGTGCAGGATTTATCAGAACTTTGGGGATTAGATAAAGATCATGTAGCAAAATTGACAGATAATATTC
CTTTTAAAGAAGCTAGTTTATTGAAGCTCAATTGCGATAAGGCATTAGCTTATTTAAATTGGCATTCTACTCTTCATTAT
GAACAATGTGTGCGTTTCATTGCAGATTGGTATAAAACTTTTTATCTTCAACAGAATATAGATATGTACCAACTTACTGT
TGCACAGATTAAGGAATATCTAGGGGAGGCTCAGAAACAGCGACTTCAATGGACAAAATGA

Upstream 100 bases:

>100_bases
TTTGGAAGCCGATGGATACACTTCGTGATAATGCAGAACTAAATGCAATGTGGGATAAGAGAAATGCTCCTTGGCAAATT
TGGAAATAAAATATATATAT

Downstream 100 bases:

>100_bases
TGTTGGGAGTTAGTATATATCCTTTAAAACAGATTCGTGTTTCAAAGGGGGATGTGTATCATGCACTAAAATCTACAGAT
TTAGGATATGTAGGGTTTGG

Product: CDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MAFNNVYQGKKVLVTGNTGFKGSWLSTWLLMMGADVYGYSSDIPTDPSMFETLKLKDKLHHCFGDIRDKAKLNEFVQKVK
PDFIFHLAAQAIVSTSYKFPFDTITTNVVGTASVLEAICNITWHCTCVLITSDKAYDNVEWIWGYRETDAMGGKDVYSGS
KGAAELVIKCYWKSFIQVMPNIKLGVARAGNVIGGGDWAKDRIIVDCINAFSQNRIVEVRCPKATRPWQHVLEPLSGYLV
LGQYLFEDKVKNGEPFNFGPRAEQTKTVFELVQDLSELWGLDKDHVAKLTDNIPFKEASLLKLNCDKALAYLNWHSTLHY
EQCVRFIADWYKTFYLQQNIDMYQLTVAQIKEYLGEAQKQRLQWTK

Sequences:

>Translated_366_residues
MAFNNVYQGKKVLVTGNTGFKGSWLSTWLLMMGADVYGYSSDIPTDPSMFETLKLKDKLHHCFGDIRDKAKLNEFVQKVK
PDFIFHLAAQAIVSTSYKFPFDTITTNVVGTASVLEAICNITWHCTCVLITSDKAYDNVEWIWGYRETDAMGGKDVYSGS
KGAAELVIKCYWKSFIQVMPNIKLGVARAGNVIGGGDWAKDRIIVDCINAFSQNRIVEVRCPKATRPWQHVLEPLSGYLV
LGQYLFEDKVKNGEPFNFGPRAEQTKTVFELVQDLSELWGLDKDHVAKLTDNIPFKEASLLKLNCDKALAYLNWHSTLHY
EQCVRFIADWYKTFYLQQNIDMYQLTVAQIKEYLGEAQKQRLQWTK
>Mature_365_residues
AFNNVYQGKKVLVTGNTGFKGSWLSTWLLMMGADVYGYSSDIPTDPSMFETLKLKDKLHHCFGDIRDKAKLNEFVQKVKP
DFIFHLAAQAIVSTSYKFPFDTITTNVVGTASVLEAICNITWHCTCVLITSDKAYDNVEWIWGYRETDAMGGKDVYSGSK
GAAELVIKCYWKSFIQVMPNIKLGVARAGNVIGGGDWAKDRIIVDCINAFSQNRIVEVRCPKATRPWQHVLEPLSGYLVL
GQYLFEDKVKNGEPFNFGPRAEQTKTVFELVQDLSELWGLDKDHVAKLTDNIPFKEASLLKLNCDKALAYLNWHSTLHYE
QCVRFIADWYKTFYLQQNIDMYQLTVAQIKEYLGEAQKQRLQWTK

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI48994969, Length=341, Percent_Identity=23.1671554252199, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1788366, Length=119, Percent_Identity=32.7731092436975, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013445
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.45 [H]

Molecular weight: Translated: 41757; Mature: 41626

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFNNVYQGKKVLVTGNTGFKGSWLSTWLLMMGADVYGYSSDIPTDPSMFETLKLKDKLH
CCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
HCFGDIRDKAKLNEFVQKVKPDFIFHLAAQAIVSTSYKFPFDTITTNVVGTASVLEAICN
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHC
ITWHCTCVLITSDKAYDNVEWIWGYRETDAMGGKDVYSGSKGAAELVIKCYWKSFIQVMP
CEEEEEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
NIKLGVARAGNVIGGGDWAKDRIIVDCINAFSQNRIVEVRCPKATRPWQHVLEPLSGYLV
CCEEEHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHH
LGQYLFEDKVKNGEPFNFGPRAEQTKTVFELVQDLSELWGLDKDHVAKLTDNIPFKEASL
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCE
LKLNCDKALAYLNWHSTLHYEQCVRFIADWYKTFYLQQNIDMYQLTVAQIKEYLGEAQKQ
EEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
RLQWTK
HCCCCC
>Mature Secondary Structure 
AFNNVYQGKKVLVTGNTGFKGSWLSTWLLMMGADVYGYSSDIPTDPSMFETLKLKDKLH
CCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
HCFGDIRDKAKLNEFVQKVKPDFIFHLAAQAIVSTSYKFPFDTITTNVVGTASVLEAICN
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHC
ITWHCTCVLITSDKAYDNVEWIWGYRETDAMGGKDVYSGSKGAAELVIKCYWKSFIQVMP
CEEEEEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
NIKLGVARAGNVIGGGDWAKDRIIVDCINAFSQNRIVEVRCPKATRPWQHVLEPLSGYLV
CCEEEHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHH
LGQYLFEDKVKNGEPFNFGPRAEQTKTVFELVQDLSELWGLDKDHVAKLTDNIPFKEASL
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCE
LKLNCDKALAYLNWHSTLHYEQCVRFIADWYKTFYLQQNIDMYQLTVAQIKEYLGEAQKQ
EEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
RLQWTK
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609 [H]