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The map label for this gene is pcoS [H]

Identifier: 29346399

GI number: 29346399

Start: 1220329

End: 1221696

Strand: Reverse

Name: pcoS [H]

Synonym: BT_0989

Alternate gene names: 29346399

Gene position: 1221696-1220329 (Counterclockwise)

Preceding gene: 29346400

Following gene: 29346398

Centisome position: 19.51

GC content: 43.06

Gene sequence:

>1368_bases
ATGAAAATCCGGACAACGCTGACTCTGCAATATGCGGGCATTACAGCTGCGGTATTCTTCGCATTTGTCATAGCTGTCTA
TTACGTAAGCGAGCATTCGCGCAGCAATGCTTTCTTTCGCAACCTGCAAAGTGAAGCTATAACCAAAGCCCACTTATTTC
TTAATAATCAGGTAGATGCAAAAACGATGCAGTCTATTTACCTGAACAATCAGAAGTTTATTAATGAAGTAGAGGTAGCC
GTCTATACCACAGATTTTAAAATACTATATCATGATGCCCTTCAAAACGACATAGTCAAGGAAACACCCGAAATGGTAAA
ACGTATTCTGAAACGAAAGAATATCAGTTTCTATGTGGACGAATATCAGGCAATAGGACTTGTGTATCCGTTTGAAGGAC
AATACTACATTGTCACTGCCGCTGCCTATGATGGCTATGGATACGCTAACCGGGATGCACTGAGAAATATGCTCATTCTG
TTATTTATAGGAGGATTGAGCGTGTTGGCGATTGTCGGTTATATCCTCTCCCGAAGCACCTTAAAGCCCATACGCAATAT
CGTGAGAGAAGCGGAGAAAATCACCGCCTCACATATCGACAAAAGACTGCCTGTAAAAAACGAACAGGACGAACTGGGAG
AACTGAGTATTACATTCAATGCACTGCTGGAACGTTTGGAGAAGTCCTTCAACTCACAAAAGATGTTTGTCAGCAATGTA
TCTCATGAATTACGTACACCTATGGCAGCCCTTACAGCCGAACTGGATCTGGCCCTGTTGAAAGAACGAACCTCCGGACA
ATATCAGGCAGCTATCGGCAATGCACTACAAGATTCGCGACGGATCATAAACCTGATCGACGGATTGCTGAATCTTGCAA
AAGCCGACTATCAGTCGGAACAGATTACAATGGAAGAGGTCCGGTTGGATGAACTGCTGTTGGATGCCAGAGAATTAGTC
CTAAAAGCCCATCCGGATTATCATATCGAATTAGTATTTGAGCAGGAAGCTGAGGAAGATAATGTGCTGACCGTAATCGG
AAACAGCTATCTGTTGACTACTGCCTTTGTGAATCTGGTAGAGAACAACTGCAAGTACTCTTCCAACCGGACTTCATCCG
TCCTGATAGCTTATTGGGAACAATGGGCTATTATCCGCTTGTCTGATAATGGCGTAGGAATGTCCGATGCAGACAAAGAA
AACCTGTTTAAACTGTTTTATCGGGGAGAAAACAAGAATATAGCTCCCGGAAATGGTATAGGCATGGTTTTGACACAGAA
AATCATTCACCTTCACAAAGGCGAACTTACCGTATCTTCTCATAAGGACGAGGGAACTACTTTTGTGGTAAAACTCCCGC
ATATCTAA

Upstream 100 bases:

>100_bases
ATGTATATATCAACTACCTTCGCAAAAAGATAGACCGTAACTTTGAACCGAAACTTATTCATACCAAAGCAGGGATGGGT
TTCATACTGACTGACAAATT

Downstream 100 bases:

>100_bases
GAAACTCTAATAGTTTTCTAATACCACTCTAATCATTCTCTAACGGCTTCCTCCCGAAGTCCTCCTTACTTTTGCATCGA
ATTAATAAATGCAGTTAGTA

Product: two-component system sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MKIRTTLTLQYAGITAAVFFAFVIAVYYVSEHSRSNAFFRNLQSEAITKAHLFLNNQVDAKTMQSIYLNNQKFINEVEVA
VYTTDFKILYHDALQNDIVKETPEMVKRILKRKNISFYVDEYQAIGLVYPFEGQYYIVTAAAYDGYGYANRDALRNMLIL
LFIGGLSVLAIVGYILSRSTLKPIRNIVREAEKITASHIDKRLPVKNEQDELGELSITFNALLERLEKSFNSQKMFVSNV
SHELRTPMAALTAELDLALLKERTSGQYQAAIGNALQDSRRIINLIDGLLNLAKADYQSEQITMEEVRLDELLLDARELV
LKAHPDYHIELVFEQEAEEDNVLTVIGNSYLLTTAFVNLVENNCKYSSNRTSSVLIAYWEQWAIIRLSDNGVGMSDADKE
NLFKLFYRGENKNIAPGNGIGMVLTQKIIHLHKGELTVSSHKDEGTTFVVKLPHI

Sequences:

>Translated_455_residues
MKIRTTLTLQYAGITAAVFFAFVIAVYYVSEHSRSNAFFRNLQSEAITKAHLFLNNQVDAKTMQSIYLNNQKFINEVEVA
VYTTDFKILYHDALQNDIVKETPEMVKRILKRKNISFYVDEYQAIGLVYPFEGQYYIVTAAAYDGYGYANRDALRNMLIL
LFIGGLSVLAIVGYILSRSTLKPIRNIVREAEKITASHIDKRLPVKNEQDELGELSITFNALLERLEKSFNSQKMFVSNV
SHELRTPMAALTAELDLALLKERTSGQYQAAIGNALQDSRRIINLIDGLLNLAKADYQSEQITMEEVRLDELLLDARELV
LKAHPDYHIELVFEQEAEEDNVLTVIGNSYLLTTAFVNLVENNCKYSSNRTSSVLIAYWEQWAIIRLSDNGVGMSDADKE
NLFKLFYRGENKNIAPGNGIGMVLTQKIIHLHKGELTVSSHKDEGTTFVVKLPHI
>Mature_455_residues
MKIRTTLTLQYAGITAAVFFAFVIAVYYVSEHSRSNAFFRNLQSEAITKAHLFLNNQVDAKTMQSIYLNNQKFINEVEVA
VYTTDFKILYHDALQNDIVKETPEMVKRILKRKNISFYVDEYQAIGLVYPFEGQYYIVTAAAYDGYGYANRDALRNMLIL
LFIGGLSVLAIVGYILSRSTLKPIRNIVREAEKITASHIDKRLPVKNEQDELGELSITFNALLERLEKSFNSQKMFVSNV
SHELRTPMAALTAELDLALLKERTSGQYQAAIGNALQDSRRIINLIDGLLNLAKADYQSEQITMEEVRLDELLLDARELV
LKAHPDYHIELVFEQEAEEDNVLTVIGNSYLLTTAFVNLVENNCKYSSNRTSSVLIAYWEQWAIIRLSDNGVGMSDADKE
NLFKLFYRGENKNIAPGNGIGMVLTQKIIHLHKGELTVSSHKDEGTTFVVKLPHI

Specific function: Probable member of a two-component regulatory system pcoS/pcoR. May activate pcoR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=279, Percent_Identity=28.3154121863799, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1788393, Length=285, Percent_Identity=28.4210526315789, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1788279, Length=299, Percent_Identity=26.4214046822742, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790346, Length=283, Percent_Identity=26.1484098939929, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI145693157, Length=237, Percent_Identity=26.1603375527426, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1789149, Length=350, Percent_Identity=24, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790551, Length=294, Percent_Identity=24.8299319727891, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786912, Length=224, Percent_Identity=24.5535714285714, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1788549, Length=224, Percent_Identity=27.2321428571429, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1786600, Length=228, Percent_Identity=25.8771929824561, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1788713, Length=231, Percent_Identity=22.0779220779221, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI48994928, Length=232, Percent_Identity=22.8448275862069, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1789403, Length=320, Percent_Identity=24.6875, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51630; Mature: 51630

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRTTLTLQYAGITAAVFFAFVIAVYYVSEHSRSNAFFRNLQSEAITKAHLFLNNQVDA
CEEEEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEECCCCCH
KTMQSIYLNNQKFINEVEVAVYTTDFKILYHDALQNDIVKETPEMVKRILKRKNISFYVD
HHHHHHHCCCHHHHCEEEEEEEEECEEEEEHHHHHHHHHHCCHHHHHHHHHHCCCEEEEE
EYQAIGLVYPFEGQYYIVTAAAYDGYGYANRDALRNMLILLFIGGLSVLAIVGYILSRST
CEEEEEEEEECCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
LKPIRNIVREAEKITASHIDKRLPVKNEQDELGELSITFNALLERLEKSFNSQKMFVSNV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCHHHHHHH
SHELRTPMAALTAELDLALLKERTSGQYQAAIGNALQDSRRIINLIDGLLNLAKADYQSE
HHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
QITMEEVRLDELLLDARELVLKAHPDYHIELVFEQEAEEDNVLTVIGNSYLLTTAFVNLV
CEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEEECCCHHHHHHHHHHH
ENNCKYSSNRTSSVLIAYWEQWAIIRLSDNGVGMSDADKENLFKLFYRGENKNIAPGNGI
HCCCCCCCCCCCEEEEEEECCEEEEEECCCCCCCCCCCHHHHEEEEEECCCCCCCCCCCC
GMVLTQKIIHLHKGELTVSSHKDEGTTFVVKLPHI
HHHHHHHHHHHHCCCEEEECCCCCCCEEEEECCCC
>Mature Secondary Structure
MKIRTTLTLQYAGITAAVFFAFVIAVYYVSEHSRSNAFFRNLQSEAITKAHLFLNNQVDA
CEEEEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEECCCCCH
KTMQSIYLNNQKFINEVEVAVYTTDFKILYHDALQNDIVKETPEMVKRILKRKNISFYVD
HHHHHHHCCCHHHHCEEEEEEEEECEEEEEHHHHHHHHHHCCHHHHHHHHHHCCCEEEEE
EYQAIGLVYPFEGQYYIVTAAAYDGYGYANRDALRNMLILLFIGGLSVLAIVGYILSRST
CEEEEEEEEECCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
LKPIRNIVREAEKITASHIDKRLPVKNEQDELGELSITFNALLERLEKSFNSQKMFVSNV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCHHHHHHH
SHELRTPMAALTAELDLALLKERTSGQYQAAIGNALQDSRRIINLIDGLLNLAKADYQSE
HHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
QITMEEVRLDELLLDARELVLKAHPDYHIELVFEQEAEEDNVLTVIGNSYLLTTAFVNLV
CEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEEECCCHHHHHHHHHHH
ENNCKYSSNRTSSVLIAYWEQWAIIRLSDNGVGMSDADKENLFKLFYRGENKNIAPGNGI
HCCCCCCCCCCCEEEEEEECCEEEEEECCCCCCCCCCCHHHHEEEEEECCCCCCCCCCCC
GMVLTQKIIHLHKGELTVSSHKDEGTTFVVKLPHI
HHHHHHHHHHHHCCCEEEECCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8594334 [H]