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The map label for this gene is btuB [H]

Identifier: 29346370

GI number: 29346370

Start: 1173791

End: 1175632

Strand: Reverse

Name: btuB [H]

Synonym: BT_0960

Alternate gene names: 29346370

Gene position: 1175632-1173791 (Counterclockwise)

Preceding gene: 29346371

Following gene: 29346369

Centisome position: 18.78

GC content: 44.35

Gene sequence:

>1842_bases
ATGGCACAACCCGGAAAAGACAGCACACAAGTCAAAAGAAACTATACTATCGACGAAGTCGTAGTTACCGGAACACGGAA
CGAGACGGATATTCGTCACCTTCCCATGACTATTTCAGTAGTAGGAAGGCAACAAATAGAGAAAAGATATGAACCTTCAC
TTTTGCCGCTATTGACGGAACAGGTTCCCGGATTCTTTACTACCAGCCGTGGTATTATGGGATATGGTGTGTCCACAGGT
GCGGCAGGTGGCATGAGTCTGCGTGGTATCGGAGGCAGTCCGACGGCAGGTTTATTGGTTTTGATCGATGGACATCCGCA
ATATATGGGATTGATGGGACATCCGATTGCGGATGCATATCAGTCTATGTTGACAGAGAAAGTGGAAGTCTTGCGCGGCC
CTGCCTCTGTACTCTATGGCTCGAATGCAATGGGCGGAGTAATCAATATAGTCACCCGCAAACAGCAGGATGAGGGAGTA
AGAACGAATATGCAGGTAGGGTATGGCTCATACAACACTTTGCAGACGGAATTTTCCAACCGTGTAAAGAAAGGACGTTT
CAGCAGTATCGTTACCGGTTCTTACAATCGTACGGACGGACATCGTCCCGATATGGAGTTCAAACAATATGGAGGATACG
CGAAGTTAGGATATGATTTCAGCTCTTCCTGGAAACTCTGGGGAGATGTAAATGTAACCCATTTCAATGCTTCCAATCCG
GGAACGATACAAGTTCCACTTATCGACAATGATTCGCGTATTACACGTGGTATGACCTCGCTTGCCTTGGAAAACCATTA
TGAGAAGACATCAGGAGCTTTGAGCCTTTTCTATAATTGGGGACGGCATAAAATCAATGACGGATATAAAACAGGAGAAC
AACCACAAACATCACACTTTAATTCTAAAGACAAGATGCTCGGCATTTCCTGGTATCAGAGTGCTACGTTCTTCACAGGC
AATCGTCTGACGGTAGGATTTGACTATCAGCACTTCGGAGGGGAATCATGGAACAAAGTCTTGGCTACCGGAGAGCGGAA
ACCGGGAGTAGACAAGCAAATGGATGAATTTGCAGGATATGTCGATTTCCGTCAGGATATAAATAGCTGGTTGTCATTGG
ATGCGGGTGTTCGTGTAGATCATCACTCTCATGTAGGTACTGAATGGATTCCACAAGGAGGACTGGCTTTTCATCTTCCC
AAAAGTGCAGAACTTAAAGCGATGGTGAGCAAAGGATATCGCAACCCTACCATCCGGGAAATGTATATGTTTGCTCCTGC
CAATCCGGAACTGAATCCGGAAAAACTGGTCAGTTATGAACTTTCCTATTCCCAACGGTTATTGGAAGATGCCTTATATT
ACGGACTTAACCTGTATTATATTAATGGTGACAATGTAATTATGAGCAACGGTTTGACGCCTCCGCTGAACGTCAATTCC
GGAGAAATAGAAAACTGGGGAATTGAAGCGAATATCGGTTATCGGTTTAATTCTCATTGGAATGTGAATGCCAACTATAG
CTGGTTGCACATGGAGAATCCGGTTCTCGCCGCTCCTGAACACAAATTGTATGTCGGAGCAGACTATACCGCAGGTCGCT
GGACTGTATCGACAGGAATGCAATATATTAAGGGACTTTACACTTCTGTAATCGTTAATAATAAAGGCACCGAGCAACAG
GATAGTTTTGTTTTATGGAATCTGCGAGGAAGTTATCGTGTTTGTAATTTTGCCAACATCTTTGTGAAAGGTGAAAACCT
GTTGGCACAACGTTACGAAATAAATGCAGGGTATCCCATGCCCAAGGCAACATTCATGGGTGGTATTAATTTGAATTTCT
AA

Upstream 100 bases:

>100_bases
AGTGGTAAAAAGTTGCAGGAGAAATAAAAGCGCGAAACAGAATATGAAAACAAATACAGTTTGTTTGTTTTTGTTCGGCA
GCCTGCTTTCCATTTCAGGA

Downstream 100 bases:

>100_bases
AAGAATAAAGATATGGAAACAACAGTAAAACTCTATTCACTGAATTACAGTAATGTGAAGACCTATTTGGCAGCCTCGTT
ATTCATCGTTGGCAATATCC

Product: putative TonB-dependent outer membrane receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 613; Mature: 612

Protein sequence:

>613_residues
MAQPGKDSTQVKRNYTIDEVVVTGTRNETDIRHLPMTISVVGRQQIEKRYEPSLLPLLTEQVPGFFTTSRGIMGYGVSTG
AAGGMSLRGIGGSPTAGLLVLIDGHPQYMGLMGHPIADAYQSMLTEKVEVLRGPASVLYGSNAMGGVINIVTRKQQDEGV
RTNMQVGYGSYNTLQTEFSNRVKKGRFSSIVTGSYNRTDGHRPDMEFKQYGGYAKLGYDFSSSWKLWGDVNVTHFNASNP
GTIQVPLIDNDSRITRGMTSLALENHYEKTSGALSLFYNWGRHKINDGYKTGEQPQTSHFNSKDKMLGISWYQSATFFTG
NRLTVGFDYQHFGGESWNKVLATGERKPGVDKQMDEFAGYVDFRQDINSWLSLDAGVRVDHHSHVGTEWIPQGGLAFHLP
KSAELKAMVSKGYRNPTIREMYMFAPANPELNPEKLVSYELSYSQRLLEDALYYGLNLYYINGDNVIMSNGLTPPLNVNS
GEIENWGIEANIGYRFNSHWNVNANYSWLHMENPVLAAPEHKLYVGADYTAGRWTVSTGMQYIKGLYTSVIVNNKGTEQQ
DSFVLWNLRGSYRVCNFANIFVKGENLLAQRYEINAGYPMPKATFMGGINLNF

Sequences:

>Translated_613_residues
MAQPGKDSTQVKRNYTIDEVVVTGTRNETDIRHLPMTISVVGRQQIEKRYEPSLLPLLTEQVPGFFTTSRGIMGYGVSTG
AAGGMSLRGIGGSPTAGLLVLIDGHPQYMGLMGHPIADAYQSMLTEKVEVLRGPASVLYGSNAMGGVINIVTRKQQDEGV
RTNMQVGYGSYNTLQTEFSNRVKKGRFSSIVTGSYNRTDGHRPDMEFKQYGGYAKLGYDFSSSWKLWGDVNVTHFNASNP
GTIQVPLIDNDSRITRGMTSLALENHYEKTSGALSLFYNWGRHKINDGYKTGEQPQTSHFNSKDKMLGISWYQSATFFTG
NRLTVGFDYQHFGGESWNKVLATGERKPGVDKQMDEFAGYVDFRQDINSWLSLDAGVRVDHHSHVGTEWIPQGGLAFHLP
KSAELKAMVSKGYRNPTIREMYMFAPANPELNPEKLVSYELSYSQRLLEDALYYGLNLYYINGDNVIMSNGLTPPLNVNS
GEIENWGIEANIGYRFNSHWNVNANYSWLHMENPVLAAPEHKLYVGADYTAGRWTVSTGMQYIKGLYTSVIVNNKGTEQQ
DSFVLWNLRGSYRVCNFANIFVKGENLLAQRYEINAGYPMPKATFMGGINLNF
>Mature_612_residues
AQPGKDSTQVKRNYTIDEVVVTGTRNETDIRHLPMTISVVGRQQIEKRYEPSLLPLLTEQVPGFFTTSRGIMGYGVSTGA
AGGMSLRGIGGSPTAGLLVLIDGHPQYMGLMGHPIADAYQSMLTEKVEVLRGPASVLYGSNAMGGVINIVTRKQQDEGVR
TNMQVGYGSYNTLQTEFSNRVKKGRFSSIVTGSYNRTDGHRPDMEFKQYGGYAKLGYDFSSSWKLWGDVNVTHFNASNPG
TIQVPLIDNDSRITRGMTSLALENHYEKTSGALSLFYNWGRHKINDGYKTGEQPQTSHFNSKDKMLGISWYQSATFFTGN
RLTVGFDYQHFGGESWNKVLATGERKPGVDKQMDEFAGYVDFRQDINSWLSLDAGVRVDHHSHVGTEWIPQGGLAFHLPK
SAELKAMVSKGYRNPTIREMYMFAPANPELNPEKLVSYELSYSQRLLEDALYYGLNLYYINGDNVIMSNGLTPPLNVNSG
EIENWGIEANIGYRFNSHWNVNANYSWLHMENPVLAAPEHKLYVGADYTAGRWTVSTGMQYIKGLYTSVIVNNKGTEQQD
SFVLWNLRGSYRVCNFANIFVKGENLLAQRYEINAGYPMPKATFMGGINLNF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=613, Percent_Identity=22.8384991843393, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788478, Length=139, Percent_Identity=34.5323741007194, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010916
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 68512; Mature: 68381

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQPGKDSTQVKRNYTIDEVVVTGTRNETDIRHLPMTISVVGRQQIEKRYEPSLLPLLTE
CCCCCCCCCCEECCCCEEEEEEECCCCCCCCEECCEEEEECCHHHHHHHCCCCHHHHHHH
QVPGFFTTSRGIMGYGVSTGAAGGMSLRGIGGSPTAGLLVLIDGHPQYMGLMGHPIADAY
HCCCEEEECCCEEEECCCCCCCCCEEEECCCCCCCCCEEEEECCCCCEECCCCCCHHHHH
QSMLTEKVEVLRGPASVLYGSNAMGGVINIVTRKQQDEGVRTNMQVGYGSYNTLQTEFSN
HHHHHHHHHHHCCCHHEEECCCCCCCEEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHH
RVKKGRFSSIVTGSYNRTDGHRPDMEFKQYGGYAKLGYDFSSSWKLWGDVNVTHFNASNP
HHHCCCCCEEEECCCCCCCCCCCCCHHHHCCCEEEEECCCCCCCEEEEEEEEEEECCCCC
GTIQVPLIDNDSRITRGMTSLALENHYEKTSGALSLFYNWGRHKINDGYKTGEQPQTSHF
CEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCC
NSKDKMLGISWYQSATFFTGNRLTVGFDYQHFGGESWNKVLATGERKPGVDKQMDEFAGY
CCCCCEEEEEEECCEEEEECCEEEEEECHHHCCCCCHHHHHCCCCCCCCCCHHHHHHHHH
VDFRQDINSWLSLDAGVRVDHHSHVGTEWIPQGGLAFHLPKSAELKAMVSKGYRNPTIRE
HHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEEECCCCCHHHHHHHCCCCCCCEEE
MYMFAPANPELNPEKLVSYELSYSQRLLEDALYYGLNLYYINGDNVIMSNGLTPPLNVNS
EEEEECCCCCCCHHHHEEEEHHHHHHHHHHHHHHCCEEEEECCCCEEEECCCCCCCCCCC
GEIENWGIEANIGYRFNSHWNVNANYSWLHMENPVLAAPEHKLYVGADYTAGRWTVSTGM
CCCCCCCEEEEEEEEECCEEEECCCEEEEEECCCEEECCCCEEEEECCCCCCCEEHHHHH
QYIKGLYTSVIVNNKGTEQQDSFVLWNLRGSYRVCNFANIFVKGENLLAQRYEINAGYPM
HHHHHHHHEEEECCCCCCCCCCEEEEEECCCEEEEEEEEEEEECCCEEEEEEECCCCCCC
PKATFMGGINLNF
CCHHEECCEEECC
>Mature Secondary Structure 
AQPGKDSTQVKRNYTIDEVVVTGTRNETDIRHLPMTISVVGRQQIEKRYEPSLLPLLTE
CCCCCCCCCEECCCCEEEEEEECCCCCCCCEECCEEEEECCHHHHHHHCCCCHHHHHHH
QVPGFFTTSRGIMGYGVSTGAAGGMSLRGIGGSPTAGLLVLIDGHPQYMGLMGHPIADAY
HCCCEEEECCCEEEECCCCCCCCCEEEECCCCCCCCCEEEEECCCCCEECCCCCCHHHHH
QSMLTEKVEVLRGPASVLYGSNAMGGVINIVTRKQQDEGVRTNMQVGYGSYNTLQTEFSN
HHHHHHHHHHHCCCHHEEECCCCCCCEEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHH
RVKKGRFSSIVTGSYNRTDGHRPDMEFKQYGGYAKLGYDFSSSWKLWGDVNVTHFNASNP
HHHCCCCCEEEECCCCCCCCCCCCCHHHHCCCEEEEECCCCCCCEEEEEEEEEEECCCCC
GTIQVPLIDNDSRITRGMTSLALENHYEKTSGALSLFYNWGRHKINDGYKTGEQPQTSHF
CEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCC
NSKDKMLGISWYQSATFFTGNRLTVGFDYQHFGGESWNKVLATGERKPGVDKQMDEFAGY
CCCCCEEEEEEECCEEEEECCEEEEEECHHHCCCCCHHHHHCCCCCCCCCCHHHHHHHHH
VDFRQDINSWLSLDAGVRVDHHSHVGTEWIPQGGLAFHLPKSAELKAMVSKGYRNPTIRE
HHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEEECCCCCHHHHHHHCCCCCCCEEE
MYMFAPANPELNPEKLVSYELSYSQRLLEDALYYGLNLYYINGDNVIMSNGLTPPLNVNS
EEEEECCCCCCCHHHHEEEEHHHHHHHHHHHHHHCCEEEEECCCCEEEECCCCCCCCCCC
GEIENWGIEANIGYRFNSHWNVNANYSWLHMENPVLAAPEHKLYVGADYTAGRWTVSTGM
CCCCCCCEEEEEEEEECCEEEECCCEEEEEECCCEEECCCCEEEEECCCCCCCEEHHHHH
QYIKGLYTSVIVNNKGTEQQDSFVLWNLRGSYRVCNFANIFVKGENLLAQRYEINAGYPM
HHHHHHHHEEEECCCCCCCCCCEEEEEECCCEEEEEEEEEEEECCCEEEEEEECCCCCCC
PKATFMGGINLNF
CCHHEECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]