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The map label for this gene is trmD [H]

Identifier: 29346303

GI number: 29346303

Start: 1095596

End: 1096273

Strand: Reverse

Name: trmD [H]

Synonym: BT_0893

Alternate gene names: 29346303

Gene position: 1096273-1095596 (Counterclockwise)

Preceding gene: 29346306

Following gene: 29346297

Centisome position: 17.51

GC content: 44.4

Gene sequence:

>678_bases
ATGCGTATTGATATTATAACAGTTTTACCCGAAATGATTGAAGGCTTCTTCAATTGCTCCATCATGAAACGAGCTCAGAA
TAAGGGACTTGCAGAAATACATATCCACAATCTGCGCGATTATACCGAAGACAAATATCGTCGTGTCGATGATTATCCTT
TTGGCGGATTCGCCGGCATGGTCATGAAGATAGAGCCTATCGAACGCTGCATTAACGCTCTGAAGGCTGAACGCGACTAT
GATGAAGTGATTTTTACGACCCCCGACGGAGAACAGTTCAATCAGCCAATGGCCAACAGCCTTTCTTTGGCCCAAAACCT
GATTATCCTTTGCGGACATTTCAAAGGTATTGATTATCGTATCCGTGAACACCTGATCACCAAAGAAATCAGTATCGGAG
ATTATGTGCTGACAGGAGGCGAACTGGCAGCAGCAGTCATGGCGGACGCAATTGTCCGTATCATCCCCGGAGTGATTTCA
GACGAACAGTCGGCACTTTCTGATTCTTTTCAGGATAATCTGTTGGCGGCACCTGTATATACTCGTCCTGCTGACTATAA
AGGCTGGAAAGTACCCGATATTTTGTTATCCGGCCATGAAGCAAAAATAAAAGAATGGGAGCTACAGCAATCTTTAGAAC
GAACCAAAAAACTTCGCCCAGACTTACTGGAAGACTAA

Upstream 100 bases:

>100_bases
CTCTATTTTTTCCTTTATATCCATATTTCTTCGATTGTTTAAGGCAAAAATACAGGTTTATCTTTGAATATTTGTACTTT
TGCGAAAAATTATAAAGATT

Downstream 100 bases:

>100_bases
AAGATATATGTAAATAAAAACGACGGTGAGAAATATGAGAATTTTCACCGTCGTTTTTATTTATATTCTACAAGAAAGAC
AAATCTGTTATTTTACTCTT

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MRIDIITVLPEMIEGFFNCSIMKRAQNKGLAEIHIHNLRDYTEDKYRRVDDYPFGGFAGMVMKIEPIERCINALKAERDY
DEVIFTTPDGEQFNQPMANSLSLAQNLIILCGHFKGIDYRIREHLITKEISIGDYVLTGGELAAAVMADAIVRIIPGVIS
DEQSALSDSFQDNLLAAPVYTRPADYKGWKVPDILLSGHEAKIKEWELQQSLERTKKLRPDLLED

Sequences:

>Translated_225_residues
MRIDIITVLPEMIEGFFNCSIMKRAQNKGLAEIHIHNLRDYTEDKYRRVDDYPFGGFAGMVMKIEPIERCINALKAERDY
DEVIFTTPDGEQFNQPMANSLSLAQNLIILCGHFKGIDYRIREHLITKEISIGDYVLTGGELAAAVMADAIVRIIPGVIS
DEQSALSDSFQDNLLAAPVYTRPADYKGWKVPDILLSGHEAKIKEWELQQSLERTKKLRPDLLED
>Mature_225_residues
MRIDIITVLPEMIEGFFNCSIMKRAQNKGLAEIHIHNLRDYTEDKYRRVDDYPFGGFAGMVMKIEPIERCINALKAERDY
DEVIFTTPDGEQFNQPMANSLSLAQNLIILCGHFKGIDYRIREHLITKEISIGDYVLTGGELAAAVMADAIVRIIPGVIS
DEQSALSDSFQDNLLAAPVYTRPADYKGWKVPDILLSGHEAKIKEWELQQSLERTKKLRPDLLED

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=226, Percent_Identity=43.3628318584071, Blast_Score=189, Evalue=1e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 25559; Mature: 25559

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIDIITVLPEMIEGFFNCSIMKRAQNKGLAEIHIHNLRDYTEDKYRRVDDYPFGGFAGM
CEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCHHHH
VMKIEPIERCINALKAERDYDEVIFTTPDGEQFNQPMANSLSLAQNLIILCGHFKGIDYR
EEEEHHHHHHHHHHHCCCCCCCEEEECCCCHHHCCHHHHHHHHHHHHHHHCCCCCCCHHH
IREHLITKEISIGDYVLTGGELAAAVMADAIVRIIPGVISDEQSALSDSFQDNLLAAPVY
HHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEECCCC
TRPADYKGWKVPDILLSGHEAKIKEWELQQSLERTKKLRPDLLED
CCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRIDIITVLPEMIEGFFNCSIMKRAQNKGLAEIHIHNLRDYTEDKYRRVDDYPFGGFAGM
CEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCHHHH
VMKIEPIERCINALKAERDYDEVIFTTPDGEQFNQPMANSLSLAQNLIILCGHFKGIDYR
EEEEHHHHHHHHHHHCCCCCCCEEEECCCCHHHCCHHHHHHHHHHHHHHHCCCCCCCHHH
IREHLITKEISIGDYVLTGGELAAAVMADAIVRIIPGVISDEQSALSDSFQDNLLAAPVY
HHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEECCCC
TRPADYKGWKVPDILLSGHEAKIKEWELQQSLERTKKLRPDLLED
CCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA