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The map label for this gene is 29346285

Identifier: 29346285

GI number: 29346285

Start: 1076614

End: 1077498

Strand: Reverse

Name: 29346285

Synonym: BT_0875

Alternate gene names: NA

Gene position: 1077498-1076614 (Counterclockwise)

Preceding gene: 29346286

Following gene: 29346280

Centisome position: 17.21

GC content: 49.04

Gene sequence:

>885_bases
ATGAAAAAGATAAAAGTAGGATTAATCCAACAATCCAATACCGCAGATATTCGGGTCAACCTGATGAACCTCGCTAAAAG
CATTGAAGCCTGTGCTGCTCACGGAGCACAACTAATCGTGCTGCAGGAGTTGCACAATTCACTTTATTTCTGCCAGACAG
AAAATACGAATCTGTTTGACCTTGCCGAGCCTATCCCAGGACCATCTACAGGTTTCTACTCCGAACTGGCAGCGGCTAAT
AAGGTAGTGCTTGTTGCTTCACTCTTTGAAAAACGCGCACCGGGCCTATATCATAACACAGCTGTCGTTTTCGACCGCGA
CGGAAGTATTGCCGGAAAATATCGGAAAATGCACATTCCCGATGATCCTGCCTATTACGAAAAGTTTTATTTCACCCCCG
GAGATATCGGTTTCGAACCGATTCAGACATCCTTAGGCAAACTGGGAGTACTGGTATGCTGGGATCAATGGTACCCGGAA
GCTGCCCGTCTGATGGCTCTGAAAGGGGCTGAACTTCTAATTTATCCTACCGCCATCGGCTGGGAAAGCAGCGATACGGA
CGACGAAAAAGCCCGCCAGCTCAATGCGTGGATAATCTCCCAACGTGCTCATGCAGTGGCAAACGGTCTTCCGGTTATCT
CTGTCAACCGTGTAGGGCACGAGCCCGACCCTTCCGGTCAAACGAACGGCATACAGTTTTGGGGAAATAGTTTCGTAGCC
GGACCGCAAGGAGAGTTTTTGGCACAGGCAAGCAACGATCATCCTGAAAACATGGTAGTTGAAATAGACATGGAACGTTC
GGAGAACGTCCGCCGCTGGTGGCCCTTCCTCCGTGACCGTCGAATTGATGAATATGACGGACTCACCAAACGTTTTTTGG
ATTAG

Upstream 100 bases:

>100_bases
AATCAAGCAACACGGTTCTCTGCATTGCGTCACCATGCAGTATCCGTCAGGTGTTATCCAATAACGGACATTTCAGTAGC
ATATTAAATAATTATTCATC

Downstream 100 bases:

>100_bases
CGAGCAGAATCACAATTAATCACCGATAGAAATAATACAAGAGGTCCACAAGAGAAAAGCCTGAATAGCAACTCTTGTGG
ACCTCCGTATTATATCCTTT

Product: beta-ureidopropionase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MKKIKVGLIQQSNTADIRVNLMNLAKSIEACAAHGAQLIVLQELHNSLYFCQTENTNLFDLAEPIPGPSTGFYSELAAAN
KVVLVASLFEKRAPGLYHNTAVVFDRDGSIAGKYRKMHIPDDPAYYEKFYFTPGDIGFEPIQTSLGKLGVLVCWDQWYPE
AARLMALKGAELLIYPTAIGWESSDTDDEKARQLNAWIISQRAHAVANGLPVISVNRVGHEPDPSGQTNGIQFWGNSFVA
GPQGEFLAQASNDHPENMVVEIDMERSENVRRWWPFLRDRRIDEYDGLTKRFLD

Sequences:

>Translated_294_residues
MKKIKVGLIQQSNTADIRVNLMNLAKSIEACAAHGAQLIVLQELHNSLYFCQTENTNLFDLAEPIPGPSTGFYSELAAAN
KVVLVASLFEKRAPGLYHNTAVVFDRDGSIAGKYRKMHIPDDPAYYEKFYFTPGDIGFEPIQTSLGKLGVLVCWDQWYPE
AARLMALKGAELLIYPTAIGWESSDTDDEKARQLNAWIISQRAHAVANGLPVISVNRVGHEPDPSGQTNGIQFWGNSFVA
GPQGEFLAQASNDHPENMVVEIDMERSENVRRWWPFLRDRRIDEYDGLTKRFLD
>Mature_294_residues
MKKIKVGLIQQSNTADIRVNLMNLAKSIEACAAHGAQLIVLQELHNSLYFCQTENTNLFDLAEPIPGPSTGFYSELAAAN
KVVLVASLFEKRAPGLYHNTAVVFDRDGSIAGKYRKMHIPDDPAYYEKFYFTPGDIGFEPIQTSLGKLGVLVCWDQWYPE
AARLMALKGAELLIYPTAIGWESSDTDDEKARQLNAWIISQRAHAVANGLPVISVNRVGHEPDPSGQTNGIQFWGNSFVA
GPQGEFLAQASNDHPENMVVEIDMERSENVRRWWPFLRDRRIDEYDGLTKRFLD

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=292, Percent_Identity=29.1095890410959, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI7706509, Length=271, Percent_Identity=28.4132841328413, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI297632348, Length=295, Percent_Identity=27.4576271186441, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI297632350, Length=295, Percent_Identity=27.4576271186441, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI5031947, Length=295, Percent_Identity=27.4576271186441, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI297632346, Length=185, Percent_Identity=29.1891891891892, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17533173, Length=291, Percent_Identity=28.8659793814433, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17556280, Length=289, Percent_Identity=25.6055363321799, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323383, Length=299, Percent_Identity=31.438127090301, Blast_Score=111, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6322335, Length=206, Percent_Identity=27.1844660194175, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21358471, Length=306, Percent_Identity=31.3725490196078, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI17933642, Length=286, Percent_Identity=26.2237762237762, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI21355835, Length=292, Percent_Identity=27.7397260273973, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 32960; Mature: 32960

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIKVGLIQQSNTADIRVNLMNLAKSIEACAAHGAQLIVLQELHNSLYFCQTENTNLFD
CCCEEEEEEECCCCCEEEEEHHHHHHHHHHHHHCCCEEEEHHHHHCCEEEEECCCCCEEE
LAEPIPGPSTGFYSELAAANKVVLVASLFEKRAPGLYHNTAVVFDRDGSIAGKYRKMHIP
CCCCCCCCCCCHHHHHHHCCCEEEEEHHHHHCCCCCEECEEEEEECCCCCCCCEEEEECC
DDPAYYEKFYFTPGDIGFEPIQTSLGKLGVLVCWDQWYPEAARLMALKGAELLIYPTAIG
CCCHHHHEEEECCCCCCCHHHHHHHHHEEEEEEECCCCCHHHHHHHCCCCEEEEEEEEEC
WESSDTDDEKARQLNAWIISQRAHAVANGLPVISVNRVGHEPDPSGQTNGIQFWGNSFVA
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEEECCEEEC
GPQGEFLAQASNDHPENMVVEIDMERSENVRRWWPFLRDRRIDEYDGLTKRFLD
CCCCCEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCHHCCHHHHHCC
>Mature Secondary Structure
MKKIKVGLIQQSNTADIRVNLMNLAKSIEACAAHGAQLIVLQELHNSLYFCQTENTNLFD
CCCEEEEEEECCCCCEEEEEHHHHHHHHHHHHHCCCEEEEHHHHHCCEEEEECCCCCEEE
LAEPIPGPSTGFYSELAAANKVVLVASLFEKRAPGLYHNTAVVFDRDGSIAGKYRKMHIP
CCCCCCCCCCCHHHHHHHCCCEEEEEHHHHHCCCCCEECEEEEEECCCCCCCCEEEEECC
DDPAYYEKFYFTPGDIGFEPIQTSLGKLGVLVCWDQWYPEAARLMALKGAELLIYPTAIG
CCCHHHHEEEECCCCCCCHHHHHHHHHEEEEEEECCCCCHHHHHHHCCCCEEEEEEEEEC
WESSDTDDEKARQLNAWIISQRAHAVANGLPVISVNRVGHEPDPSGQTNGIQFWGNSFVA
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEEECCEEEC
GPQGEFLAQASNDHPENMVVEIDMERSENVRRWWPFLRDRRIDEYDGLTKRFLD
CCCCCEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCHHCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]