Click here to switch to the map view.

The map label for this gene is pyrB

Identifier: 29346152

GI number: 29346152

Start: 919435

End: 920376

Strand: Reverse

Name: pyrB

Synonym: BT_0742

Alternate gene names: 29346152

Gene position: 920376-919435 (Counterclockwise)

Preceding gene: 29346158

Following gene: 29346151

Centisome position: 14.7

GC content: 47.66

Gene sequence:

>942_bases
ATGGAAAACAGAAGTTTAGTAACCATTGCCGAACATTCTAAAGAAAAAATCCTCTACATGCTCGAAATGGCGAAGCAGTT
TGAAATGAACCCCAACCGCCGGTTATTGCAAGGTAAGGTAGTAGCAACCCTGTTCTTCGAACCTTCCACCCGTACCCGTT
TGAGTTTCGAAACAGCAGCCAATCGCCTCGGCGCACGCGTCATCGGATTTACTGATCCGAAAGCAACCAGCTCCTCAAAA
GGAGAAACACTCAAAGACACGATCATGATGGTTAGCAGCTATGCGGATATTATCGTCATGCGACACTATCTCGAAGGAGC
TGCCCGATATGCCAGCGAAGTAGCACCCGTACCCATCGTCAATGCAGGAGACGGAGCCAACCAGCACCCGTCACAAACGA
TGCTCGACCTCTACTCCATCTACAAAACTCAGGGAACACTGGAAAACCTGAACATCTTTCTGGTGGGAGACTTGAAATAC
GGACGCACCGTCCACTCACTACTGATGGCTATGCGTCACTTCAATCCTACATTCCACTTCATCGCCCCCGATGAACTGAA
AATGCCGGAAGAATATAAGCTATACTGCAAAGAGCATCAGATCAAATACATTGAACATACCGAATTTACCGAAGAAATTA
TCGCTGATGCCGACATCCTGTACATGACCCGTGTGCAACGCGAACGCTTCACCGACCTGATGGAATACGAACGAGTGAAA
AATGTATATATCCTGCGCAACAAGATGCTGGAGAACACCCGTCCGAACCTCCGCATTCTGCACCCGCTGCCCCGTGTCAA
CGAGATTGCTTACGACGTGGACAACAACCCGAAAGCATATTATTTCCAACAGGCGCAAAACGGTCTGTATGCCCGTGAAG
CCATCCTTTGCGACGTATTAGGTATCACATTAGAGGACGTTAAAAACGATATTTTATTATGA

Upstream 100 bases:

>100_bases
CGAAAAACGAATAAAAAGAGAAAAAAAGTTTGTATTTTACATTTTCTTCGTACCTTTACACTCCGGTAAAACAAGTTCTA
TCCACATAAAAGATAACAAA

Downstream 100 bases:

>100_bases
GCGAAAATAAACAAGCATTGCAAGTAGCTGCCCTGAAAAACGGGACAGTGATTGACCATATCCCATCAGAAAAACTCTTT
ACTGTCGTACAGCTACTGGG

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MENRSLVTIAEHSKEKILYMLEMAKQFEMNPNRRLLQGKVVATLFFEPSTRTRLSFETAANRLGARVIGFTDPKATSSSK
GETLKDTIMMVSSYADIIVMRHYLEGAARYASEVAPVPIVNAGDGANQHPSQTMLDLYSIYKTQGTLENLNIFLVGDLKY
GRTVHSLLMAMRHFNPTFHFIAPDELKMPEEYKLYCKEHQIKYIEHTEFTEEIIADADILYMTRVQRERFTDLMEYERVK
NVYILRNKMLENTRPNLRILHPLPRVNEIAYDVDNNPKAYYFQQAQNGLYAREAILCDVLGITLEDVKNDILL

Sequences:

>Translated_313_residues
MENRSLVTIAEHSKEKILYMLEMAKQFEMNPNRRLLQGKVVATLFFEPSTRTRLSFETAANRLGARVIGFTDPKATSSSK
GETLKDTIMMVSSYADIIVMRHYLEGAARYASEVAPVPIVNAGDGANQHPSQTMLDLYSIYKTQGTLENLNIFLVGDLKY
GRTVHSLLMAMRHFNPTFHFIAPDELKMPEEYKLYCKEHQIKYIEHTEFTEEIIADADILYMTRVQRERFTDLMEYERVK
NVYILRNKMLENTRPNLRILHPLPRVNEIAYDVDNNPKAYYFQQAQNGLYAREAILCDVLGITLEDVKNDILL
>Mature_313_residues
MENRSLVTIAEHSKEKILYMLEMAKQFEMNPNRRLLQGKVVATLFFEPSTRTRLSFETAANRLGARVIGFTDPKATSSSK
GETLKDTIMMVSSYADIIVMRHYLEGAARYASEVAPVPIVNAGDGANQHPSQTMLDLYSIYKTQGTLENLNIFLVGDLKY
GRTVHSLLMAMRHFNPTFHFIAPDELKMPEEYKLYCKEHQIKYIEHTEFTEEIIADADILYMTRVQRERFTDLMEYERVK
NVYILRNKMLENTRPNLRILHPLPRVNEIAYDVDNNPKAYYFQQAQNGLYAREAILCDVLGITLEDVKNDILL

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=304, Percent_Identity=39.1447368421053, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI38788445, Length=313, Percent_Identity=24.6006389776358, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI2367364, Length=300, Percent_Identity=53, Blast_Score=317, Evalue=9e-88,
Organism=Escherichia coli, GI1786469, Length=297, Percent_Identity=24.9158249158249, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790703, Length=300, Percent_Identity=24.6666666666667, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI193204318, Length=321, Percent_Identity=38.6292834890966, Blast_Score=213, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6322331, Length=306, Percent_Identity=40.1960784313725, Blast_Score=251, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6322373, Length=313, Percent_Identity=23.961661341853, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24642586, Length=271, Percent_Identity=42.4354243542435, Blast_Score=235, Evalue=2e-62,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_BACTN (Q8A9S3)

Other databases:

- EMBL:   AE015928
- RefSeq:   NP_809655.1
- ProteinModelPortal:   Q8A9S3
- SMR:   Q8A9S3
- GeneID:   1073579
- GenomeReviews:   AE015928_GR
- KEGG:   bth:BT_0742
- NMPDR:   fig|226186.1.peg.742
- HOGENOM:   HBG579429
- OMA:   AQYIDSH
- PhylomeDB:   Q8A9S3
- ProtClustDB:   PRK00856
- BioCyc:   BTHE226186:BT_0742-MONOMER
- BRENDA:   2.1.3.2
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 36188; Mature: 36188

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENRSLVTIAEHSKEKILYMLEMAKQFEMNPNRRLLQGKVVATLFFEPSTRTRLSFETAA
CCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCEEHHCEEEEEEEECCCCCCEEEHHHHH
NRLGARVIGFTDPKATSSSKGETLKDTIMMVSSYADIIVMRHYLEGAARYASEVAPVPIV
HHHCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
NAGDGANQHPSQTMLDLYSIYKTQGTLENLNIFLVGDLKYGRTVHSLLMAMRHFNPTFHF
ECCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCEEEE
IAPDELKMPEEYKLYCKEHQIKYIEHTEFTEEIIADADILYMTRVQRERFTDLMEYERVK
ECCHHCCCCHHHHHHHHHCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
NVYILRNKMLENTRPNLRILHPLPRVNEIAYDVDNNPKAYYFQQAQNGLYAREAILCDVL
HHHHHHHHHHHCCCCCEEEECCCCCCCCEEEECCCCCCEEEEEHHCCCHHHHHHHHHHHH
GITLEDVKNDILL
HCCHHHHHHHCCC
>Mature Secondary Structure
MENRSLVTIAEHSKEKILYMLEMAKQFEMNPNRRLLQGKVVATLFFEPSTRTRLSFETAA
CCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCEEHHCEEEEEEEECCCCCCEEEHHHHH
NRLGARVIGFTDPKATSSSKGETLKDTIMMVSSYADIIVMRHYLEGAARYASEVAPVPIV
HHHCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
NAGDGANQHPSQTMLDLYSIYKTQGTLENLNIFLVGDLKYGRTVHSLLMAMRHFNPTFHF
ECCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCEEEE
IAPDELKMPEEYKLYCKEHQIKYIEHTEFTEEIIADADILYMTRVQRERFTDLMEYERVK
ECCHHCCCCHHHHHHHHHCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
NVYILRNKMLENTRPNLRILHPLPRVNEIAYDVDNNPKAYYFQQAQNGLYAREAILCDVL
HHHHHHHHHHHCCCCCEEEECCCCCCCCEEEECCCCCCEEEEEHHCCCHHHHHHHHHHHH
GITLEDVKNDILL
HCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928