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The map label for this gene is prs [H]

Identifier: 29346158

GI number: 29346158

Start: 926690

End: 927607

Strand: Reverse

Name: prs [H]

Synonym: BT_0748

Alternate gene names: 29346158

Gene position: 927607-926690 (Counterclockwise)

Preceding gene: 29346169

Following gene: 29346152

Centisome position: 14.82

GC content: 45.75

Gene sequence:

>918_bases
ATGGTATTCTCGGGAACTAACTCGAGATATCTTGCAGAGAAAATCTGCGCAAGTCTCAATTGTCCTCTGGGAAACATGAA
CATCACCCACTTTGCAGATGGTGAGTTTGCCGTTTCTTACGAAGAATCAATTCGTGGCGCACATGTATTCCTTGTGCAGT
CTACTTTCCCTAACTCAGACAACTTAATGGAACTTCTCCTGATGATCGACGCCGCCAAACGTGCATCTGCAAAGAGCGTC
GTAGCTGTTATCCCCTATTTCGGATGGGCACGTCAGGACAGAAAAGACAAACCTCGTGTATCTATCGGAGCTAAATTGGT
AGCCGACCTGCTTTCAGTAGCAGGTATCGACCGACTGATTACCATGGATTTGCATGCAGACCAGATTCAGGGATTCTTCA
ATATCCCCGTAGATCACCTGTATGCATCAGCCGTATTCCTCCCCTATATCCAGTCATTGAAACTGGAAGACCTGGTGATT
GCTACACCGGACGTAGGTGGTTCAAAACGCGCCAGCACTTTCTCCAAATACCTTGGTGTACCTTTGGTACTCTGCAACAA
GTCACGTGAAAAAGCCAATGAAGTAGCTTCCATGCAAATCATCGGTGATGTGAAAGACAAAAACGTAGTATTGATCGATG
ACATCGTAGATACAGCAGGCACCATCACCAAAGCTGCCAATATCATGATGGAAGCCGGAGCCAAATCCGTACGCGCTATT
GCCAGCCACTGTGTAATGTCTGACCCTGCTTCTTTCCGTGTGCAGGAATCCGGATTGACTGAAATGGTATTTACAGACAG
TATCCCTTACGCTAAGAAATGCGCGAAAGTGAAACAACTGAGCATCGCTGATATGTTTGCAGAAACAATCAAGCGGGTAA
TGAATAACGAGTCCATCAGTTCACAATATATCATCTAA

Upstream 100 bases:

>100_bases
TACAATAAGTCTTTCTAAATAATAAGAGAAATTCCTACCTTTGCAGGCAAATTAACAAATAGGTAACATCTATTACAAAA
TGAGCGAAAAAGCACCCTTT

Downstream 100 bases:

>100_bases
ATGAAGAATGATGAGTAAAGGATAAGATAAAGAAAGCCGGCTAAACTTGAATTTAGCCGGCTTTCTTATTTATCTATAAT
TCTTTCTTTTAAAGAAGTCT

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MVFSGTNSRYLAEKICASLNCPLGNMNITHFADGEFAVSYEESIRGAHVFLVQSTFPNSDNLMELLLMIDAAKRASAKSV
VAVIPYFGWARQDRKDKPRVSIGAKLVADLLSVAGIDRLITMDLHADQIQGFFNIPVDHLYASAVFLPYIQSLKLEDLVI
ATPDVGGSKRASTFSKYLGVPLVLCNKSREKANEVASMQIIGDVKDKNVVLIDDIVDTAGTITKAANIMMEAGAKSVRAI
ASHCVMSDPASFRVQESGLTEMVFTDSIPYAKKCAKVKQLSIADMFAETIKRVMNNESISSQYII

Sequences:

>Translated_305_residues
MVFSGTNSRYLAEKICASLNCPLGNMNITHFADGEFAVSYEESIRGAHVFLVQSTFPNSDNLMELLLMIDAAKRASAKSV
VAVIPYFGWARQDRKDKPRVSIGAKLVADLLSVAGIDRLITMDLHADQIQGFFNIPVDHLYASAVFLPYIQSLKLEDLVI
ATPDVGGSKRASTFSKYLGVPLVLCNKSREKANEVASMQIIGDVKDKNVVLIDDIVDTAGTITKAANIMMEAGAKSVRAI
ASHCVMSDPASFRVQESGLTEMVFTDSIPYAKKCAKVKQLSIADMFAETIKRVMNNESISSQYII
>Mature_305_residues
MVFSGTNSRYLAEKICASLNCPLGNMNITHFADGEFAVSYEESIRGAHVFLVQSTFPNSDNLMELLLMIDAAKRASAKSV
VAVIPYFGWARQDRKDKPRVSIGAKLVADLLSVAGIDRLITMDLHADQIQGFFNIPVDHLYASAVFLPYIQSLKLEDLVI
ATPDVGGSKRASTFSKYLGVPLVLCNKSREKANEVASMQIIGDVKDKNVVLIDDIVDTAGTITKAANIMMEAGAKSVRAI
ASHCVMSDPASFRVQESGLTEMVFTDSIPYAKKCAKVKQLSIADMFAETIKRVMNNESISSQYII

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506127, Length=305, Percent_Identity=46.5573770491803, Blast_Score=266, Evalue=2e-71,
Organism=Homo sapiens, GI4506129, Length=306, Percent_Identity=45.0980392156863, Blast_Score=266, Evalue=2e-71,
Organism=Homo sapiens, GI84875539, Length=309, Percent_Identity=44.6601941747573, Blast_Score=260, Evalue=1e-69,
Organism=Homo sapiens, GI28557709, Length=305, Percent_Identity=44.9180327868852, Blast_Score=257, Evalue=8e-69,
Organism=Homo sapiens, GI4506133, Length=334, Percent_Identity=32.3353293413174, Blast_Score=165, Evalue=6e-41,
Organism=Homo sapiens, GI194018537, Length=327, Percent_Identity=32.4159021406728, Blast_Score=157, Evalue=8e-39,
Organism=Homo sapiens, GI310128524, Length=149, Percent_Identity=33.5570469798658, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI310115209, Length=149, Percent_Identity=33.5570469798658, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI310118259, Length=149, Percent_Identity=33.5570469798658, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI310119946, Length=149, Percent_Identity=33.5570469798658, Blast_Score=82, Evalue=7e-16,
Organism=Escherichia coli, GI1787458, Length=308, Percent_Identity=43.8311688311688, Blast_Score=267, Evalue=8e-73,
Organism=Caenorhabditis elegans, GI17554704, Length=305, Percent_Identity=45.5737704918033, Blast_Score=257, Evalue=4e-69,
Organism=Caenorhabditis elegans, GI25149168, Length=305, Percent_Identity=45.5737704918033, Blast_Score=257, Evalue=4e-69,
Organism=Caenorhabditis elegans, GI17554702, Length=305, Percent_Identity=45.5737704918033, Blast_Score=257, Evalue=6e-69,
Organism=Caenorhabditis elegans, GI71989924, Length=305, Percent_Identity=45.5737704918033, Blast_Score=256, Evalue=9e-69,
Organism=Caenorhabditis elegans, GI17570245, Length=336, Percent_Identity=28.8690476190476, Blast_Score=164, Evalue=7e-41,
Organism=Saccharomyces cerevisiae, GI6319403, Length=304, Percent_Identity=42.4342105263158, Blast_Score=250, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6321776, Length=298, Percent_Identity=44.2953020134228, Blast_Score=249, Evalue=4e-67,
Organism=Saccharomyces cerevisiae, GI6320946, Length=303, Percent_Identity=42.2442244224422, Blast_Score=244, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6322667, Length=199, Percent_Identity=32.6633165829146, Blast_Score=129, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6324511, Length=87, Percent_Identity=47.1264367816092, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI21355239, Length=305, Percent_Identity=48.1967213114754, Blast_Score=275, Evalue=3e-74,
Organism=Drosophila melanogaster, GI45551540, Length=328, Percent_Identity=44.8170731707317, Blast_Score=261, Evalue=3e-70,
Organism=Drosophila melanogaster, GI24651458, Length=349, Percent_Identity=29.512893982808, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24651456, Length=349, Percent_Identity=29.512893982808, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI281362873, Length=349, Percent_Identity=29.512893982808, Blast_Score=163, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24651454, Length=349, Percent_Identity=29.512893982808, Blast_Score=163, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24651462, Length=368, Percent_Identity=28.5326086956522, Blast_Score=157, Evalue=6e-39,
Organism=Drosophila melanogaster, GI24651464, Length=368, Percent_Identity=28.5326086956522, Blast_Score=157, Evalue=6e-39,
Organism=Drosophila melanogaster, GI45552010, Length=368, Percent_Identity=28.5326086956522, Blast_Score=157, Evalue=7e-39,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 33299; Mature: 33299

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00114 PRPP_SYNTHETASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFSGTNSRYLAEKICASLNCPLGNMNITHFADGEFAVSYEESIRGAHVFLVQSTFPNSD
CEECCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEHHHHCCCEEEEEEEECCCCCH
NLMELLLMIDAAKRASAKSVVAVIPYFGWARQDRKDKPRVSIGAKLVADLLSVAGIDRLI
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHEE
TMDLHADQIQGFFNIPVDHLYASAVFLPYIQSLKLEDLVIATPDVGGSKRASTFSKYLGV
EEECCHHHHCCEECCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHCC
PLVLCNKSREKANEVASMQIIGDVKDKNVVLIDDIVDTAGTITKAANIMMEAGAKSVRAI
EEEEECCCHHHHHHHHHEEEEECCCCCCEEEEECHHCCHHHHHHHHHHHHHHCHHHHHHH
ASHCVMSDPASFRVQESGLTEMVFTDSIPYAKKCAKVKQLSIADMFAETIKRVMNNESIS
HHHHHCCCCCCEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SQYII
CCCCC
>Mature Secondary Structure
MVFSGTNSRYLAEKICASLNCPLGNMNITHFADGEFAVSYEESIRGAHVFLVQSTFPNSD
CEECCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEHHHHCCCEEEEEEEECCCCCH
NLMELLLMIDAAKRASAKSVVAVIPYFGWARQDRKDKPRVSIGAKLVADLLSVAGIDRLI
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHEE
TMDLHADQIQGFFNIPVDHLYASAVFLPYIQSLKLEDLVIATPDVGGSKRASTFSKYLGV
EEECCHHHHCCEECCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHCC
PLVLCNKSREKANEVASMQIIGDVKDKNVVLIDDIVDTAGTITKAANIMMEAGAKSVRAI
EEEEECCCHHHHHHHHHEEEEECCCCCCEEEEECHHCCHHHHHHHHHHHHHHCHHHHHHH
ASHCVMSDPASFRVQESGLTEMVFTDSIPYAKKCAKVKQLSIADMFAETIKRVMNNESIS
HHHHHCCCCCCEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SQYII
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12949112 [H]