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The map label for this gene is thiC

Identifier: 29346060

GI number: 29346060

Start: 800221

End: 801918

Strand: Reverse

Name: thiC

Synonym: BT_0650

Alternate gene names: 29346060

Gene position: 801918-800221 (Counterclockwise)

Preceding gene: 29346061

Following gene: 29346059

Centisome position: 12.81

GC content: 48.23

Gene sequence:

>1698_bases
ATGGAACAAAAAATAAAATTCCCCCGTTCTCAGAAAGTGTATCTGCCGGGTAAACTCTATCCGAACATCCGTGTAGCCAT
GCGGAAAGTTGAACAAGTGCCCAGTGTCAGCTTCGAAGGAGAAGAAAAGATAGCTACTCCGAATCCGGAAGTATATGTAT
ATGACACCAGCGGTCCGTTCAGTGACCCGTCAATGAGCATTGATCTTAAGAAAGGACTTCCCCGCTTACGTGAAGAATGG
ATTGTGGGACGCGGTGACGTAGAGCAACTCCCCGAAATCACTTCGGAATACGGACAAATGAGACGTGATGACAAAAGTCT
GGATCATCTCCGCTTTGAACACATTGCCCTTCCCTATCGTGCCAAAAAGGGAGAAGCCATCACTCAGATGGCATACGCCA
AACGAGGAATCATCACTCCGGAAATGGAATATGTGGCGATCCGCGAAAATATGAACTGTGAGGAACTGGGAATCGAGACT
CACATTACTCCCGAATTTGTCCGTAAAGAAATAGCAGAAGGACATGCAGTGCTGCCTGCCAACATCAATCATCCGGAAGC
CGAACCGATGATTATCGGCCGTAACTTCCTAGTGAAAATCAATACGAATATCGGAAACTCTGCCACCACTTCCAGCATTG
ACGAGGAAGTGGAAAAAGCTTTATGGAGCTGCAAATGGGGAGGTGATACGCTGATGGACCTTTCAACAGGAGAAAATATT
CATGAGACACGTGAATGGATCATCCGCAACTGCCCTGTACCGGTAGGCACTGTGCCTATCTATCAGGCACTTGAGAAAGT
GAATGGCGTAGTGGAAGACCTTAACTGGGAAATCTATCGGGACACACTGATCGAACAATGCGAACAGGGAGTAGATTATT
TTACGATTCATGCAGGTATCCGCCGTCATAATGTTCATCTTGCCGATAAACGTCTGTGCGGCATTGTCAGCCGTGGAGGA
AGCATCATGAGTAAATGGTGTCTGGTGCACGATCAGGAAAGTTTCCTGTATGAACACTTTGACGATATCTGTGATATTCT
GGCTCAATACGACGTTGCCGTATCATTGGGAGACGGACTGCGTCCGGGCTCTATCCATGATGCCAACGACGAAGCACAGT
TTGCAGAACTGGACACGATGGGAGAACTGGTACTTCGTGCATGGGAAAAGAACGTGCAGGCATTTATCGAAGGTCCGGGA
CACGTACCGATGCATAAAATCAAGGAGAACATGGAACGTCAGATCGAGAAATGCCATGATGCGCCATTTTATACACTCGG
CCCTTTAGTGACGGATATCGCTCCTGGCTACGATCACATTACCTCTGCCATCGGAGCTGCTCAAATCGGCTGGTTAGGTA
CAGCTATGCTTTGTTATGTCACTCCGAAAGAGCATCTTGCCCTGCCGGATAAAGAAGACGTACGTGTAGGGGTTATCACT
TATAAGATTGCCGCTCATGCTGCCGACCTTGCCAAAGGGCATCCGGGAGCACAGGTACGCGACAATGCCTTGAGCAAAGC
CCGTTACGAGTTCCGCTGGAAAGATCAGTTCGACTTGTCTCTCGATCCGGAACGGGCACAGGGATATTTCCGCGCGGGAC
ATCACATTGACGGAGAATACTGCACGATGTGCGGACCAAATTTCTGCGCCATGAGATTATCCCGTGATCTGAAGAAAAAT
GCCAAGAGTGATAAATAA

Upstream 100 bases:

>100_bases
TAACTGCATTTTTGGAATAAGTAATAGGTGATAAGTAATAGGTAATAAGTAGGGAGTAGTGTTTGTTTCACTATTCTTAA
TGATATGATAATAAAATTCT

Downstream 100 bases:

>100_bases
TGAGTTGTGAATATCCCCAAACCAAGTCACAACCTCATTTCTTTTTAATTTTCAACTTTCAATTAATTAAAATGTTCTCA
GACGAATTAGAAAAAATATC

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 565; Mature: 565

Protein sequence:

>565_residues
MEQKIKFPRSQKVYLPGKLYPNIRVAMRKVEQVPSVSFEGEEKIATPNPEVYVYDTSGPFSDPSMSIDLKKGLPRLREEW
IVGRGDVEQLPEITSEYGQMRRDDKSLDHLRFEHIALPYRAKKGEAITQMAYAKRGIITPEMEYVAIRENMNCEELGIET
HITPEFVRKEIAEGHAVLPANINHPEAEPMIIGRNFLVKINTNIGNSATTSSIDEEVEKALWSCKWGGDTLMDLSTGENI
HETREWIIRNCPVPVGTVPIYQALEKVNGVVEDLNWEIYRDTLIEQCEQGVDYFTIHAGIRRHNVHLADKRLCGIVSRGG
SIMSKWCLVHDQESFLYEHFDDICDILAQYDVAVSLGDGLRPGSIHDANDEAQFAELDTMGELVLRAWEKNVQAFIEGPG
HVPMHKIKENMERQIEKCHDAPFYTLGPLVTDIAPGYDHITSAIGAAQIGWLGTAMLCYVTPKEHLALPDKEDVRVGVIT
YKIAAHAADLAKGHPGAQVRDNALSKARYEFRWKDQFDLSLDPERAQGYFRAGHHIDGEYCTMCGPNFCAMRLSRDLKKN
AKSDK

Sequences:

>Translated_565_residues
MEQKIKFPRSQKVYLPGKLYPNIRVAMRKVEQVPSVSFEGEEKIATPNPEVYVYDTSGPFSDPSMSIDLKKGLPRLREEW
IVGRGDVEQLPEITSEYGQMRRDDKSLDHLRFEHIALPYRAKKGEAITQMAYAKRGIITPEMEYVAIRENMNCEELGIET
HITPEFVRKEIAEGHAVLPANINHPEAEPMIIGRNFLVKINTNIGNSATTSSIDEEVEKALWSCKWGGDTLMDLSTGENI
HETREWIIRNCPVPVGTVPIYQALEKVNGVVEDLNWEIYRDTLIEQCEQGVDYFTIHAGIRRHNVHLADKRLCGIVSRGG
SIMSKWCLVHDQESFLYEHFDDICDILAQYDVAVSLGDGLRPGSIHDANDEAQFAELDTMGELVLRAWEKNVQAFIEGPG
HVPMHKIKENMERQIEKCHDAPFYTLGPLVTDIAPGYDHITSAIGAAQIGWLGTAMLCYVTPKEHLALPDKEDVRVGVIT
YKIAAHAADLAKGHPGAQVRDNALSKARYEFRWKDQFDLSLDPERAQGYFRAGHHIDGEYCTMCGPNFCAMRLSRDLKKN
AKSDK
>Mature_565_residues
MEQKIKFPRSQKVYLPGKLYPNIRVAMRKVEQVPSVSFEGEEKIATPNPEVYVYDTSGPFSDPSMSIDLKKGLPRLREEW
IVGRGDVEQLPEITSEYGQMRRDDKSLDHLRFEHIALPYRAKKGEAITQMAYAKRGIITPEMEYVAIRENMNCEELGIET
HITPEFVRKEIAEGHAVLPANINHPEAEPMIIGRNFLVKINTNIGNSATTSSIDEEVEKALWSCKWGGDTLMDLSTGENI
HETREWIIRNCPVPVGTVPIYQALEKVNGVVEDLNWEIYRDTLIEQCEQGVDYFTIHAGIRRHNVHLADKRLCGIVSRGG
SIMSKWCLVHDQESFLYEHFDDICDILAQYDVAVSLGDGLRPGSIHDANDEAQFAELDTMGELVLRAWEKNVQAFIEGPG
HVPMHKIKENMERQIEKCHDAPFYTLGPLVTDIAPGYDHITSAIGAAQIGWLGTAMLCYVTPKEHLALPDKEDVRVGVIT
YKIAAHAADLAKGHPGAQVRDNALSKARYEFRWKDQFDLSLDPERAQGYFRAGHHIDGEYCTMCGPNFCAMRLSRDLKKN
AKSDK

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=583, Percent_Identity=60.7204116638079, Blast_Score=748, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_BACTN (Q8AA15)

Other databases:

- EMBL:   AE015928
- RefSeq:   NP_809563.1
- GeneID:   1072410
- GenomeReviews:   AE015928_GR
- KEGG:   bth:BT_0650
- NMPDR:   fig|226186.1.peg.650
- HOGENOM:   HBG285234
- OMA:   TWELFRD
- PhylomeDB:   Q8AA15
- ProtClustDB:   PRK09284
- BioCyc:   BTHE226186:BT_0650-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 63833; Mature: 63833

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: BINDING 203-203 BINDING 232-232 BINDING 261-261 BINDING 297-297 BINDING 397-397 BINDING 424-424

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQKIKFPRSQKVYLPGKLYPNIRVAMRKVEQVPSVSFEGEEKIATPNPEVYVYDTSGPF
CCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCC
SDPSMSIDLKKGLPRLREEWIVGRGDVEQLPEITSEYGQMRRDDKSLDHLRFEHIALPYR
CCCCCEEEHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHEECCCC
AKKGEAITQMAYAKRGIITPEMEYVAIRENMNCEELGIETHITPEFVRKEIAEGHAVLPA
CCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHCCCCCCCCHHHHHHHHHCCCEEEEC
NINHPEAEPMIIGRNFLVKINTNIGNSATTSSIDEEVEKALWSCKWGGDTLMDLSTGENI
CCCCCCCCCEEECCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCH
HETREWIIRNCPVPVGTVPIYQALEKVNGVVEDLNWEIYRDTLIEQCEQGVDYFTIHAGI
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEECCC
RRHNVHLADKRLCGIVSRGGSIMSKWCLVHDQESFLYEHFDDICDILAQYDVAVSLGDGL
EECCCEEHHHHHHHHHHCCCHHHHHHHHEECHHHHHHHHHHHHHHHHHHHCEEEEECCCC
RPGSIHDANDEAQFAELDTMGELVLRAWEKNVQAFIEGPGHVPMHKIKENMERQIEKCHD
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHC
APFYTLGPLVTDIAPGYDHITSAIGAAQIGWLGTAMLCYVTPKEHLALPDKEDVRVGVIT
CCCEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCCCCCEEEEEE
YKIAAHAADLAKGHPGAQVRDNALSKARYEFRWKDQFDLSLDPERAQGYFRAGHHIDGEY
EEHHHHHHHHHCCCCCCCHHHHHHHHHHEEEEECCCCCCCCCHHHHCCHHHCCCCCCCCE
CTMCGPNFCAMRLSRDLKKNAKSDK
EECCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MEQKIKFPRSQKVYLPGKLYPNIRVAMRKVEQVPSVSFEGEEKIATPNPEVYVYDTSGPF
CCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCC
SDPSMSIDLKKGLPRLREEWIVGRGDVEQLPEITSEYGQMRRDDKSLDHLRFEHIALPYR
CCCCCEEEHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHEECCCC
AKKGEAITQMAYAKRGIITPEMEYVAIRENMNCEELGIETHITPEFVRKEIAEGHAVLPA
CCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHCCCCCCCCHHHHHHHHHCCCEEEEC
NINHPEAEPMIIGRNFLVKINTNIGNSATTSSIDEEVEKALWSCKWGGDTLMDLSTGENI
CCCCCCCCCEEECCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCH
HETREWIIRNCPVPVGTVPIYQALEKVNGVVEDLNWEIYRDTLIEQCEQGVDYFTIHAGI
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEECCC
RRHNVHLADKRLCGIVSRGGSIMSKWCLVHDQESFLYEHFDDICDILAQYDVAVSLGDGL
EECCCEEHHHHHHHHHHCCCHHHHHHHHEECHHHHHHHHHHHHHHHHHHHCEEEEECCCC
RPGSIHDANDEAQFAELDTMGELVLRAWEKNVQAFIEGPGHVPMHKIKENMERQIEKCHD
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHC
APFYTLGPLVTDIAPGYDHITSAIGAAQIGWLGTAMLCYVTPKEHLALPDKEDVRVGVIT
CCCEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCCCCCEEEEEE
YKIAAHAADLAKGHPGAQVRDNALSKARYEFRWKDQFDLSLDPERAQGYFRAGHHIDGEY
EEHHHHHHHHHCCCCCCCHHHHHHHHHHEEEEECCCCCCCCCHHHHCCHHHCCCCCCCCE
CTMCGPNFCAMRLSRDLKKNAKSDK
EECCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663928