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The map label for this gene is thiG

Identifier: 29346061

GI number: 29346061

Start: 801999

End: 802772

Strand: Reverse

Name: thiG

Synonym: BT_0651

Alternate gene names: 29346061

Gene position: 802772-801999 (Counterclockwise)

Preceding gene: 29346062

Following gene: 29346060

Centisome position: 12.82

GC content: 50.65

Gene sequence:

>774_bases
GTGGAAAAGTTAGTAATTGCAGGCCGTGAGTTCGACTCCCGCCTTTTTCTGGGTACAGGAAAATTCAATTCCAATGAAGT
AATGGAACAAGCCATTCTGGCATCGGGCACTGAAATGGTAACCGTAGCCATGAAGCGCATCGACATGGATGACAAAGAAG
ACGACATGCTGAAACATATTATTCATCCGAATATCCAGTTGCTACCCAACACATCGGGAGTACGCGATGCGGAAGAAGCT
GTTTTCGCCGCACAGATGGCACGTGAAGCTTTTGGTACCAACTGGCTGAAACTGGAAATCCATCCGGATCCGCGCTATCT
GCTCCCCGACTCTATCGAAACCCTAAAGGCTACAGAACAGCTTGTTAAACTGGGCTTTATCGTATTACCCTATTGCCAGG
CCGACCCTGTGCTCTGCAAACGACTGGAAGAAGCCGGTGCCGCTACCGTAATGCCGCTGGGTGCTCCCATCGGAACTAAC
AAAGGACTGCAAACCAAAGAATTCCTGCAGATTATTATCGAACAGGCCGGTATCCCCGTTGTCGTAGATGCCGGAATCGG
TGCACCGAGCCACGCTGCCGAAGCAATGGAACTGGGTGCTTCTGCCGTACTGGTAAATACCGCTATCGCCGTAGCAGGCA
ATCCGGTAGAAATGGCGTTGGCCTTCAAAGCCGCCACCGAAGCCGGAAGACGGGCATACGAAGCAGGACTGGGTCTGCAG
GCTGATAACTTTATAGCAGAAGCAAGTTCACCTTTAACTGCATTTTTGGAATAA

Upstream 100 bases:

>100_bases
AATCGCGCTTTCAGGAACGATTCTTCGGGCGGAGGATCCGGCAGCGGAAACACGAAAGATTTTGAACATGAAACGTATAA
TCAAATAAACTTATTGAAAA

Downstream 100 bases:

>100_bases
GTAATAGGTGATAAGTAATAGGTAATAAGTAGGGAGTAGTGTTTGTTTCACTATTCTTAATGATATGATAATAAAATTCT
ATGGAACAAAAAATAAAATT

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MEKLVIAGREFDSRLFLGTGKFNSNEVMEQAILASGTEMVTVAMKRIDMDDKEDDMLKHIIHPNIQLLPNTSGVRDAEEA
VFAAQMAREAFGTNWLKLEIHPDPRYLLPDSIETLKATEQLVKLGFIVLPYCQADPVLCKRLEEAGAATVMPLGAPIGTN
KGLQTKEFLQIIIEQAGIPVVVDAGIGAPSHAAEAMELGASAVLVNTAIAVAGNPVEMALAFKAATEAGRRAYEAGLGLQ
ADNFIAEASSPLTAFLE

Sequences:

>Translated_257_residues
MEKLVIAGREFDSRLFLGTGKFNSNEVMEQAILASGTEMVTVAMKRIDMDDKEDDMLKHIIHPNIQLLPNTSGVRDAEEA
VFAAQMAREAFGTNWLKLEIHPDPRYLLPDSIETLKATEQLVKLGFIVLPYCQADPVLCKRLEEAGAATVMPLGAPIGTN
KGLQTKEFLQIIIEQAGIPVVVDAGIGAPSHAAEAMELGASAVLVNTAIAVAGNPVEMALAFKAATEAGRRAYEAGLGLQ
ADNFIAEASSPLTAFLE
>Mature_257_residues
MEKLVIAGREFDSRLFLGTGKFNSNEVMEQAILASGTEMVTVAMKRIDMDDKEDDMLKHIIHPNIQLLPNTSGVRDAEEA
VFAAQMAREAFGTNWLKLEIHPDPRYLLPDSIETLKATEQLVKLGFIVLPYCQADPVLCKRLEEAGAATVMPLGAPIGTN
KGLQTKEFLQIIIEQAGIPVVVDAGIGAPSHAAEAMELGASAVLVNTAIAVAGNPVEMALAFKAATEAGRRAYEAGLGLQ
ADNFIAEASSPLTAFLE

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=254, Percent_Identity=64.5669291338583, Blast_Score=335, Evalue=2e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_BACTN (Q8AA14)

Other databases:

- EMBL:   AE015928
- RefSeq:   NP_809564.1
- ProteinModelPortal:   Q8AA14
- SMR:   Q8AA14
- GeneID:   1072392
- GenomeReviews:   AE015928_GR
- KEGG:   bth:BT_0651
- NMPDR:   fig|226186.1.peg.651
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- PhylomeDB:   Q8AA14
- ProtClustDB:   PRK00208
- BioCyc:   BTHE226186:BT_0651-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 27517; Mature: 27517

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: ACT_SITE 97-97 BINDING 158-158

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLVIAGREFDSRLFLGTGKFNSNEVMEQAILASGTEMVTVAMKRIDMDDKEDDMLKHI
CCCEEEECCCCCCEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHH
IHPNIQLLPNTSGVRDAEEAVFAAQMAREAFGTNWLKLEIHPDPRYLLPDSIETLKATEQ
HCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEECCCHHHHHHHHHH
LVKLGFIVLPYCQADPVLCKRLEEAGAATVMPLGAPIGTNKGLQTKEFLQIIIEQAGIPV
HHHHHHHEEECCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCE
VVDAGIGAPSHAAEAMELGASAVLVNTAIAVAGNPVEMALAFKAATEAGRRAYEAGLGLQ
EEECCCCCCHHHHHHHHHCCHHEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
ADNFIAEASSPLTAFLE
CCHHHHHCCCCCEECCC
>Mature Secondary Structure
MEKLVIAGREFDSRLFLGTGKFNSNEVMEQAILASGTEMVTVAMKRIDMDDKEDDMLKHI
CCCEEEECCCCCCEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHH
IHPNIQLLPNTSGVRDAEEAVFAAQMAREAFGTNWLKLEIHPDPRYLLPDSIETLKATEQ
HCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEECCCHHHHHHHHHH
LVKLGFIVLPYCQADPVLCKRLEEAGAATVMPLGAPIGTNKGLQTKEFLQIIIEQAGIPV
HHHHHHHEEECCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCE
VVDAGIGAPSHAAEAMELGASAVLVNTAIAVAGNPVEMALAFKAATEAGRRAYEAGLGLQ
EEECCCCCCHHHHHHHHHCCHHEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
ADNFIAEASSPLTAFLE
CCHHHHHCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928