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The map label for this gene is gltB [H]

Identifier: 29345962

GI number: 29345962

Start: 678852

End: 680192

Strand: Reverse

Name: gltB [H]

Synonym: BT_0552

Alternate gene names: 29345962

Gene position: 680192-678852 (Counterclockwise)

Preceding gene: 29345963

Following gene: 29345961

Centisome position: 10.87

GC content: 48.92

Gene sequence:

>1341_bases
ATGGGAGATCCTAAAGCTTTTTTAAATATACCTCGGCAAGAGGCGGGATACCGCCCCGTTAGCGAGCGCATCACTGACTA
TAGTCAGGTAGAACAAACATTGAATACGAACAGCCGTAAATTACAGGCATCACGGTGTATGGATTGCGGAGTACCTTTTT
GTCACTGGGCTTGTCCAATAGGCAATAAACAGCCGGAATGGCAGGACGCTTTATTCAAAGGAAAATGGAAAGAAGCGTAT
GAAGTGCTGTCATCCACTTGCGACTTTCCGGAATTTACAGGACGTATCTGCCCTGCCCTCTGTGAGAAATCCTGTGTACT
GAAACTATCCTGTGACCAACCGGTGACTATCCGTGAAAATGAAGCTGCCATTGTAGAAGCCGCCTTCCGTGAAGGATATA
TACAGGTGCAGCATCCCCAAAGAAACGGCAAAAAAGTAGCAGTTGTCGGTGCCGGTCCGGCCGGATTGGTAGTTGCCAAC
CAACTGAATCTAAAAGGATACACCGTAACGTTATTCGATAAAGATGAAGCTGCGGGAGGATTATTACGTTTCGGTATTCC
AAACTTCAAACTGGACAAAAACGTAATCGACCGCCGCATGAAAATACTTGCCGCAGAAGGTATTCAGTTTGAAATGGGAG
TAGAAATAGACGTTAACCATTTGCCGGAAGGATTCGATGCATATTGCATTTGCACGGGTACACCGACTGCCCGTGATTTA
AGCATTCCGGGACGGGAGTTGAAAGGAATCCATTTCGCACTGGAAATGCTGGCGCAACAGAACAGGATTCTGGAAGGACA
AACTTTCCCCAAAGACAAACTGGTGAATGCCAAAGGCAAAAAAGTGCTTGTGATCGGCGGTGGAGACACCGGTTCGGACT
GTATCGGAACCAGTGTACGACAGGGAGCAACCAGCGTCACGCAAATTGAAATTATGCCCAAACCTCCCGTCGGCCACAAT
CCTTCTACGCCCTGGCCGCAATGGCCGGTAGTTTTCAAAACGACCTCCAGCCATGAAGAAGGTTGTACCCGTCGCTGGTG
CCTGACTTCCAATCAGTTTTTGGGTAAGAACGGTAAAGTAACCGGCGTCGAGGTGGAAGAAGTAGAATGGACACCGGCAA
CAGATGGCGGACGTCCGACCATGAACCTCACAGGGAAGAAAGAAGTAATCGAAGCAGATATGGTGCTGCTGGCCATGGGA
TTCCTGAAACCGGAACAACCGAAATTTGCAGAAAACGTATTCCTTGCCGGAGATGCAGCTACCGGAGCAAGCCTCGTAGT
ACGGGCGATGGCAGGAGGACGAAAGGCTGCTGCGGACATAGATCACTATTTGGTGAAGTAA

Upstream 100 bases:

>100_bases
ATTAAGTATTAGGTATTAACCGTAGCTTCAAACATCTTTAATCCATATAGTTAATACTTATTGCTTAATACCTATTACCT
AATACTTATTACTTAACAAG

Downstream 100 bases:

>100_bases
CCCTTACAGCAAGGAACACCATGAAGTTTATAGACTATTATTTATTCGTTTGACCTAGGTTGAACGAAACAACAACCTAG
GTTGAACTAAAAGATGACCC

Product: glutamate synthase small subunit

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MGDPKAFLNIPRQEAGYRPVSERITDYSQVEQTLNTNSRKLQASRCMDCGVPFCHWACPIGNKQPEWQDALFKGKWKEAY
EVLSSTCDFPEFTGRICPALCEKSCVLKLSCDQPVTIRENEAAIVEAAFREGYIQVQHPQRNGKKVAVVGAGPAGLVVAN
QLNLKGYTVTLFDKDEAAGGLLRFGIPNFKLDKNVIDRRMKILAAEGIQFEMGVEIDVNHLPEGFDAYCICTGTPTARDL
SIPGRELKGIHFALEMLAQQNRILEGQTFPKDKLVNAKGKKVLVIGGGDTGSDCIGTSVRQGATSVTQIEIMPKPPVGHN
PSTPWPQWPVVFKTTSSHEEGCTRRWCLTSNQFLGKNGKVTGVEVEEVEWTPATDGGRPTMNLTGKKEVIEADMVLLAMG
FLKPEQPKFAENVFLAGDAATGASLVVRAMAGGRKAAADIDHYLVK

Sequences:

>Translated_446_residues
MGDPKAFLNIPRQEAGYRPVSERITDYSQVEQTLNTNSRKLQASRCMDCGVPFCHWACPIGNKQPEWQDALFKGKWKEAY
EVLSSTCDFPEFTGRICPALCEKSCVLKLSCDQPVTIRENEAAIVEAAFREGYIQVQHPQRNGKKVAVVGAGPAGLVVAN
QLNLKGYTVTLFDKDEAAGGLLRFGIPNFKLDKNVIDRRMKILAAEGIQFEMGVEIDVNHLPEGFDAYCICTGTPTARDL
SIPGRELKGIHFALEMLAQQNRILEGQTFPKDKLVNAKGKKVLVIGGGDTGSDCIGTSVRQGATSVTQIEIMPKPPVGHN
PSTPWPQWPVVFKTTSSHEEGCTRRWCLTSNQFLGKNGKVTGVEVEEVEWTPATDGGRPTMNLTGKKEVIEADMVLLAMG
FLKPEQPKFAENVFLAGDAATGASLVVRAMAGGRKAAADIDHYLVK
>Mature_445_residues
GDPKAFLNIPRQEAGYRPVSERITDYSQVEQTLNTNSRKLQASRCMDCGVPFCHWACPIGNKQPEWQDALFKGKWKEAYE
VLSSTCDFPEFTGRICPALCEKSCVLKLSCDQPVTIRENEAAIVEAAFREGYIQVQHPQRNGKKVAVVGAGPAGLVVANQ
LNLKGYTVTLFDKDEAAGGLLRFGIPNFKLDKNVIDRRMKILAAEGIQFEMGVEIDVNHLPEGFDAYCICTGTPTARDLS
IPGRELKGIHFALEMLAQQNRILEGQTFPKDKLVNAKGKKVLVIGGGDTGSDCIGTSVRQGATSVTQIEIMPKPPVGHNP
STPWPQWPVVFKTTSSHEEGCTRRWCLTSNQFLGKNGKVTGVEVEEVEWTPATDGGRPTMNLTGKKEVIEADMVLLAMGF
LKPEQPKFAENVFLAGDAATGASLVVRAMAGGRKAAADIDHYLVK

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=297, Percent_Identity=25.2525252525253, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1789606, Length=483, Percent_Identity=33.1262939958592, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI1788811, Length=470, Percent_Identity=31.063829787234, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI87082180, Length=470, Percent_Identity=32.7659574468085, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1788468, Length=427, Percent_Identity=26.463700234192, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1789243, Length=258, Percent_Identity=31.7829457364341, Blast_Score=98, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17570289, Length=485, Percent_Identity=39.3814432989691, Blast_Score=347, Evalue=8e-96,
Organism=Saccharomyces cerevisiae, GI6320030, Length=481, Percent_Identity=38.6694386694387, Blast_Score=345, Evalue=9e-96,
Organism=Drosophila melanogaster, GI28574881, Length=479, Percent_Identity=43.4237995824635, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24665539, Length=479, Percent_Identity=43.4237995824635, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24665547, Length=479, Percent_Identity=43.4237995824635, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24665543, Length=479, Percent_Identity=43.4237995824635, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24640763, Length=310, Percent_Identity=24.5161290322581, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI18858217, Length=310, Percent_Identity=24.5161290322581, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 48741; Mature: 48610

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDPKAFLNIPRQEAGYRPVSERITDYSQVEQTLNTNSRKLQASRCMDCGVPFCHWACPI
CCCCHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHEEECCC
GNKQPEWQDALFKGKWKEAYEVLSSTCDFPEFTGRICPALCEKSCVLKLSCDQPVTIREN
CCCCCCHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCEEEECC
EAAIVEAAFREGYIQVQHPQRNGKKVAVVGAGPAGLVVANQLNLKGYTVTLFDKDEAAGG
CHHHHHHHHHCCEEEEECCCCCCCEEEEEECCCCCEEEEEEECCCEEEEEEEECCCCCCC
LLRFGIPNFKLDKNVIDRRMKILAAEGIQFEMGVEIDVNHLPEGFDAYCICTGTPTARDL
EEEECCCCCEECHHHHHHHHHHHHHCCEEEEECCEEECCCCCCCCCEEEEECCCCCCCCC
SIPGRELKGIHFALEMLAQQNRILEGQTFPKDKLVNAKGKKVLVIGGGDTGSDCIGTSVR
CCCCHHHHHHHHHHHHHHHHCCEECCCCCCHHHEECCCCCEEEEEECCCCCCHHHCCHHH
QGATSVTQIEIMPKPPVGHNPSTPWPQWPVVFKTTSSHEEGCTRRWCLTSNQFLGKNGKV
CCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCEECCCCCE
TGVEVEEVEWTPATDGGRPTMNLTGKKEVIEADMVLLAMGFLKPEQPKFAENVFLAGDAA
EEEEEEEEEECCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCC
TGASLVVRAMAGGRKAAADIDHYLVK
CCHHHHHHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
GDPKAFLNIPRQEAGYRPVSERITDYSQVEQTLNTNSRKLQASRCMDCGVPFCHWACPI
CCCHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHEEECCC
GNKQPEWQDALFKGKWKEAYEVLSSTCDFPEFTGRICPALCEKSCVLKLSCDQPVTIREN
CCCCCCHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCEEEECC
EAAIVEAAFREGYIQVQHPQRNGKKVAVVGAGPAGLVVANQLNLKGYTVTLFDKDEAAGG
CHHHHHHHHHCCEEEEECCCCCCCEEEEEECCCCCEEEEEEECCCEEEEEEEECCCCCCC
LLRFGIPNFKLDKNVIDRRMKILAAEGIQFEMGVEIDVNHLPEGFDAYCICTGTPTARDL
EEEECCCCCEECHHHHHHHHHHHHHCCEEEEECCEEECCCCCCCCCEEEEECCCCCCCCC
SIPGRELKGIHFALEMLAQQNRILEGQTFPKDKLVNAKGKKVLVIGGGDTGSDCIGTSVR
CCCCHHHHHHHHHHHHHHHHCCEECCCCCCHHHEECCCCCEEEEEECCCCCCHHHCCHHH
QGATSVTQIEIMPKPPVGHNPSTPWPQWPVVFKTTSSHEEGCTRRWCLTSNQFLGKNGKV
CCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCEECCCCCE
TGVEVEEVEWTPATDGGRPTMNLTGKKEVIEADMVLLAMGFLKPEQPKFAENVFLAGDAA
EEEEEEEEEECCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCC
TGASLVVRAMAGGRKAAADIDHYLVK
CCHHHHHHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]