Click here to switch to the map view.

The map label for this gene is asnB [H]

Identifier: 29345961

GI number: 29345961

Start: 676966

End: 678645

Strand: Reverse

Name: asnB [H]

Synonym: BT_0551

Alternate gene names: 29345961

Gene position: 678645-676966 (Counterclockwise)

Preceding gene: 29345962

Following gene: 29345960

Centisome position: 10.84

GC content: 46.43

Gene sequence:

>1680_bases
ATGTGTGGAATAGCAGGTATACTCAACATAAAAACTCAAACCAAAGAGCTAAGAGACAAAGCACTGAAAATGGCACAGAA
AATCCGTCATCGCGGACCGGACTGGAGCGGTATCTACGTAGGTGGAAGCGCCATTCTGGCACACGAACGCCTCTCGATAG
TTGACCCGCAAAGCGGAGGACAGCCCTTATACTCCCCCGACCGGAAACAAGCGCTCGCCGTGAACGGTGAAATCTACAAC
CATCGGGATATCCGCGCCCGCTATGCCGGACAATATGATTTTCAGACCGGAAGCGACTGTGAAGTGATTCTTGCCCTTTA
TAAAGATAAAGGCATCCACTTTCTGGAAGAGATCAGCGGAATTTTCGCCTTTGTACTTTATGATGAAGAAAAAGACGAGT
TCCTCATTGCACGTGATCCTATCGGAGTCATACCTTTATATATAGGAAAAGACAAGGATGGCAAAATCTACTTCGGCAGT
GAACTGAAAGCACTCGAAGGATTCTGTAATGAGTACGAACCATTTCTTCCGGGACATTACTTTTACAGTAAAGAAGGCAA
AATGAAGCGTTGGTATACACGCGAATGGACAGACTATGAGTCTGTAAAAGACAATGATGCCAAAGTCAGTGATGTAAAAG
AAGCATTGGAAGATGCCGTTCATCGCCAGCTGATGAGTGACGTTCCCTATGGAGTACTTCTTTCAGGTGGTCTGGACAGT
TCTGTGATTTCAGCTATCGCCAAAAAATATGCAGCAAAACGTATTGAAACAGACGGAGCAAGTGACGCCTGGTGGCCTCA
ACTACATTCTTTTGCCATCGGTCTGAAGGGGGCGCCCGATTTGATAAAGGCACGTGAAGTGGCAGAATATATCGGTACGG
TTCATCATGAAATCAATTATACGGTTCAGGAAGGGCTGGATGCTATTCGGGATGTTATCTATTTTATCGAGACGTATGAT
GTGACTACGGTACGTGCCTCCACTCCCATGTATTTACTGGCACGTGTGATCAAATCGATGGGGATCAAAATGGTATTGAG
TGGTGAAGGTGCCGATGAAGTTTTCGGCGGTTATCTTTACTTTCACAAAGCTCCCACTCCGAAAGATTTCCATGAGGAAA
CGGTACGTAAGTTATCAAAACTGCATATGTATGATTGCCTTCGTGCCAATAAGAGTTTATCAGCCTGGGGTGTAGAGGGA
CGTGTCCCATTTCTGGACAAGGAATTTCTGGATGTAGCGATGGCGTTGAATCCAAAAGCTAAAATGTGCCCCGGAAAGGA
GATTGAGAAACGAATTGTACGTGAAGCTTTTGCCGATATGCTACCGGAAAGCGTAGCATGGAGACAAAAAGAGCAGTTCA
GTGATGGTGTGGGATATTCGTGGATTGATACCTTAAGAGAGATTACAGCGGCTGCCGTCAGTGATGAACAGATGGAACAC
GCTGCCGAACGTTTCCCGATTAACACTCCGCTCAACAAGGAAGAATATTACTACCGGAGTATCTTTGAAGAGCATTTTCC
GAGTGAAAGCGCAGCCCGCAGCGTACCAAGCGTACCAAGTGTGGCCTGCTCTACAGCAGAAGCGCTGGCATGGGATGTAG
CTTTCCGTAACCTGAATGAACCAAGCGGCAGAGCAGTGAGAGGAGTGCATGAAGAAGCCTATGCCGAAGAGGTAAAATAA

Upstream 100 bases:

>100_bases
AACGAAAGGAAAAGCCAGGGAAAACGAATAAATAGTAACGAACCGACTTTTAAAAAACAGAGAATCAACAAATTATATTA
ACTTCCAAATACTCAAAGTT

Downstream 100 bases:

>100_bases
ATTTGTTGAAAAATTTTAGATGTCTGAAATAAACTCATATCTTTGAATATATATTCATCAGTTTGTAAAGTCAGGAATAA
AAAGTATAGACTTCTATGCA

Product: asparagine synthetase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 559; Mature: 559

Protein sequence:

>559_residues
MCGIAGILNIKTQTKELRDKALKMAQKIRHRGPDWSGIYVGGSAILAHERLSIVDPQSGGQPLYSPDRKQALAVNGEIYN
HRDIRARYAGQYDFQTGSDCEVILALYKDKGIHFLEEISGIFAFVLYDEEKDEFLIARDPIGVIPLYIGKDKDGKIYFGS
ELKALEGFCNEYEPFLPGHYFYSKEGKMKRWYTREWTDYESVKDNDAKVSDVKEALEDAVHRQLMSDVPYGVLLSGGLDS
SVISAIAKKYAAKRIETDGASDAWWPQLHSFAIGLKGAPDLIKAREVAEYIGTVHHEINYTVQEGLDAIRDVIYFIETYD
VTTVRASTPMYLLARVIKSMGIKMVLSGEGADEVFGGYLYFHKAPTPKDFHEETVRKLSKLHMYDCLRANKSLSAWGVEG
RVPFLDKEFLDVAMALNPKAKMCPGKEIEKRIVREAFADMLPESVAWRQKEQFSDGVGYSWIDTLREITAAAVSDEQMEH
AAERFPINTPLNKEEYYYRSIFEEHFPSESAARSVPSVPSVACSTAEALAWDVAFRNLNEPSGRAVRGVHEEAYAEEVK

Sequences:

>Translated_559_residues
MCGIAGILNIKTQTKELRDKALKMAQKIRHRGPDWSGIYVGGSAILAHERLSIVDPQSGGQPLYSPDRKQALAVNGEIYN
HRDIRARYAGQYDFQTGSDCEVILALYKDKGIHFLEEISGIFAFVLYDEEKDEFLIARDPIGVIPLYIGKDKDGKIYFGS
ELKALEGFCNEYEPFLPGHYFYSKEGKMKRWYTREWTDYESVKDNDAKVSDVKEALEDAVHRQLMSDVPYGVLLSGGLDS
SVISAIAKKYAAKRIETDGASDAWWPQLHSFAIGLKGAPDLIKAREVAEYIGTVHHEINYTVQEGLDAIRDVIYFIETYD
VTTVRASTPMYLLARVIKSMGIKMVLSGEGADEVFGGYLYFHKAPTPKDFHEETVRKLSKLHMYDCLRANKSLSAWGVEG
RVPFLDKEFLDVAMALNPKAKMCPGKEIEKRIVREAFADMLPESVAWRQKEQFSDGVGYSWIDTLREITAAAVSDEQMEH
AAERFPINTPLNKEEYYYRSIFEEHFPSESAARSVPSVPSVACSTAEALAWDVAFRNLNEPSGRAVRGVHEEAYAEEVK
>Mature_559_residues
MCGIAGILNIKTQTKELRDKALKMAQKIRHRGPDWSGIYVGGSAILAHERLSIVDPQSGGQPLYSPDRKQALAVNGEIYN
HRDIRARYAGQYDFQTGSDCEVILALYKDKGIHFLEEISGIFAFVLYDEEKDEFLIARDPIGVIPLYIGKDKDGKIYFGS
ELKALEGFCNEYEPFLPGHYFYSKEGKMKRWYTREWTDYESVKDNDAKVSDVKEALEDAVHRQLMSDVPYGVLLSGGLDS
SVISAIAKKYAAKRIETDGASDAWWPQLHSFAIGLKGAPDLIKAREVAEYIGTVHHEINYTVQEGLDAIRDVIYFIETYD
VTTVRASTPMYLLARVIKSMGIKMVLSGEGADEVFGGYLYFHKAPTPKDFHEETVRKLSKLHMYDCLRANKSLSAWGVEG
RVPFLDKEFLDVAMALNPKAKMCPGKEIEKRIVREAFADMLPESVAWRQKEQFSDGVGYSWIDTLREITAAAVSDEQMEH
AAERFPINTPLNKEEYYYRSIFEEHFPSESAARSVPSVPSVACSTAEALAWDVAFRNLNEPSGRAVRGVHEEAYAEEVK

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=545, Percent_Identity=37.2477064220184, Blast_Score=325, Evalue=8e-89,
Organism=Homo sapiens, GI168229252, Length=545, Percent_Identity=37.2477064220184, Blast_Score=325, Evalue=8e-89,
Organism=Homo sapiens, GI168229250, Length=545, Percent_Identity=37.2477064220184, Blast_Score=325, Evalue=8e-89,
Organism=Homo sapiens, GI296010848, Length=519, Percent_Identity=38.150289017341, Blast_Score=322, Evalue=6e-88,
Organism=Homo sapiens, GI296010852, Length=451, Percent_Identity=38.8026607538803, Blast_Score=290, Evalue=2e-78,
Organism=Homo sapiens, GI296010850, Length=451, Percent_Identity=38.8026607538803, Blast_Score=290, Evalue=2e-78,
Organism=Escherichia coli, GI1786889, Length=554, Percent_Identity=65.3429602888087, Blast_Score=771, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25147557, Length=570, Percent_Identity=44.2105263157895, Blast_Score=452, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI71993933, Length=532, Percent_Identity=45.6766917293233, Blast_Score=448, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI17560178, Length=519, Percent_Identity=35.8381502890173, Blast_Score=294, Evalue=7e-80,
Organism=Caenorhabditis elegans, GI25147560, Length=348, Percent_Identity=37.6436781609195, Blast_Score=214, Evalue=8e-56,
Organism=Saccharomyces cerevisiae, GI6321563, Length=584, Percent_Identity=47.0890410958904, Blast_Score=516, Evalue=1e-147,
Organism=Saccharomyces cerevisiae, GI6325403, Length=584, Percent_Identity=46.4041095890411, Blast_Score=508, Evalue=1e-145,
Organism=Drosophila melanogaster, GI45553209, Length=576, Percent_Identity=46.875, Blast_Score=484, Evalue=1e-137,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 63020; Mature: 63020

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIAGILNIKTQTKELRDKALKMAQKIRHRGPDWSGIYVGGSAILAHERLSIVDPQSGG
CCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEECCCEEEECCCCCC
QPLYSPDRKQALAVNGEIYNHRDIRARYAGQYDFQTGSDCEVILALYKDKGIHFLEEISG
CCCCCCCCCCEEEECCEEECCCHHHHHCCCCCCCCCCCCHHEEHEEHHCCCCHHHHHHHH
IFAFVLYDEEKDEFLIARDPIGVIPLYIGKDKDGKIYFGSELKALEGFCNEYEPFLPGHY
HEEEEEEECCCCCEEEEECCCCEEEEEECCCCCCCEEECCHHHHHHHHHHCCCCCCCCCE
FYSKEGKMKRWYTREWTDYESVKDNDAKVSDVKEALEDAVHRQLMSDVPYGVLLSGGLDS
EECCCCCCCHHHCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCH
SVISAIAKKYAAKRIETDGASDAWWPQLHSFAIGLKGAPDLIKAREVAEYIGTVHHEINY
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCH
TVQEGLDAIRDVIYFIETYDVTTVRASTPMYLLARVIKSMGIKMVLSGEGADEVFGGYLY
HHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCEEEEEECCCCHHHHCCEEE
FHKAPTPKDFHEETVRKLSKLHMYDCLRANKSLSAWGVEGRVPFLDKEFLDVAMALNPKA
EECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
KMCPGKEIEKRIVREAFADMLPESVAWRQKEQFSDGVGYSWIDTLREITAAAVSDEQMEH
CCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
AAERFPINTPLNKEEYYYRSIFEEHFPSESAARSVPSVPSVACSTAEALAWDVAFRNLNE
HHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCC
PSGRAVRGVHEEAYAEEVK
CCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MCGIAGILNIKTQTKELRDKALKMAQKIRHRGPDWSGIYVGGSAILAHERLSIVDPQSGG
CCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEECCCEEEECCCCCC
QPLYSPDRKQALAVNGEIYNHRDIRARYAGQYDFQTGSDCEVILALYKDKGIHFLEEISG
CCCCCCCCCCEEEECCEEECCCHHHHHCCCCCCCCCCCCHHEEHEEHHCCCCHHHHHHHH
IFAFVLYDEEKDEFLIARDPIGVIPLYIGKDKDGKIYFGSELKALEGFCNEYEPFLPGHY
HEEEEEEECCCCCEEEEECCCCEEEEEECCCCCCCEEECCHHHHHHHHHHCCCCCCCCCE
FYSKEGKMKRWYTREWTDYESVKDNDAKVSDVKEALEDAVHRQLMSDVPYGVLLSGGLDS
EECCCCCCCHHHCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCH
SVISAIAKKYAAKRIETDGASDAWWPQLHSFAIGLKGAPDLIKAREVAEYIGTVHHEINY
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCH
TVQEGLDAIRDVIYFIETYDVTTVRASTPMYLLARVIKSMGIKMVLSGEGADEVFGGYLY
HHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCEEEEEECCCCHHHHCCEEE
FHKAPTPKDFHEETVRKLSKLHMYDCLRANKSLSAWGVEGRVPFLDKEFLDVAMALNPKA
EECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
KMCPGKEIEKRIVREAFADMLPESVAWRQKEQFSDGVGYSWIDTLREITAAAVSDEQMEH
CCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
AAERFPINTPLNKEEYYYRSIFEEHFPSESAARSVPSVPSVACSTAEALAWDVAFRNLNE
HHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCC
PSGRAVRGVHEEAYAEEVK
CCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1973930; 8905232; 9278503; 10587437 [H]