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The map label for this gene is glnB [H]

Identifier: 29345955

GI number: 29345955

Start: 671772

End: 672128

Strand: Reverse

Name: glnB [H]

Synonym: BT_0545

Alternate gene names: 29345955

Gene position: 672128-671772 (Counterclockwise)

Preceding gene: 29345956

Following gene: 29345954

Centisome position: 10.74

GC content: 48.18

Gene sequence:

>357_bases
ATGAAAAAGATTGAAGCTATCATCCGTAAAACCAAATTCGAGGACGTGAAAGACGCTCTGCTCGAAGCGGACATTGAATG
GTTCTCTTACTACGATGTAAGAGGGATCGGTAAAGCACGGCAAGGACGTATCTATCGTGGAGTGGTATATGATACCAGCA
CCATCGAACGTATTCTGGTTTCTATTGTAGTACGCGACAAAAATGCAGAAAAGACCGTACAGGCTATCATCAAAGCCGCA
CAAACCGGAGAGATCGGTGACGGACGCATTTTCGTCATTCCTATCGAGGACGCCATCCGCATCCGCACCGCCGAACGTGG
CGACATCGCTCTCTATAACGCCGAACAGGAACGATAA

Upstream 100 bases:

>100_bases
TAGAGTTTAGATTTTATCGGTTTGTTGTATGGTAACCTGCGATGGCTGCCACCTCCCTATACCTCTTCTTTTTACAGGTC
AACACTAATATTTAAGGTAT

Downstream 100 bases:

>100_bases
CCGGCAACCGGCAAGGTGCCGGGGCCAGATTTCCTTTCGGATGATAATATTATAAAAACAGGTATTAAATAAGATAATCA
TTATGGATAAAACATATAAG

Product: nitrogen regulatory protein P-II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 118; Mature: 118

Protein sequence:

>118_residues
MKKIEAIIRKTKFEDVKDALLEADIEWFSYYDVRGIGKARQGRIYRGVVYDTSTIERILVSIVVRDKNAEKTVQAIIKAA
QTGEIGDGRIFVIPIEDAIRIRTAERGDIALYNAEQER

Sequences:

>Translated_118_residues
MKKIEAIIRKTKFEDVKDALLEADIEWFSYYDVRGIGKARQGRIYRGVVYDTSTIERILVSIVVRDKNAEKTVQAIIKAA
QTGEIGDGRIFVIPIEDAIRIRTAERGDIALYNAEQER
>Mature_118_residues
MKKIEAIIRKTKFEDVKDALLEADIEWFSYYDVRGIGKARQGRIYRGVVYDTSTIERILVSIVVRDKNAEKTVQAIIKAA
QTGEIGDGRIFVIPIEDAIRIRTAERGDIALYNAEQER

Specific function: In nitrogen-limiting conditions, when the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP. P- II-UMP allows the deadenylation of glutamine synthetase (GS), thus activating the enzyme. Conversely, in nitrogen excess P-II is deur

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family [H]

Homologues:

Organism=Escherichia coli, GI1788904, Length=112, Percent_Identity=41.9642857142857, Blast_Score=84, Evalue=2e-18,
Organism=Escherichia coli, GI1786655, Length=112, Percent_Identity=34.8214285714286, Blast_Score=75, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918 [H]

Pfam domain/function: PF00543 P-II [H]

EC number: NA

Molecular weight: Translated: 13474; Mature: 13474

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: PS00638 PII_GLNB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIEAIIRKTKFEDVKDALLEADIEWFSYYDVRGIGKARQGRIYRGVVYDTSTIERILV
CCHHHHHHHHCCHHHHHHHHHHHCHHHHEEECCCCCCCCCCCCEEEEEEECHHHHHHHHH
SIVVRDKNAEKTVQAIIKAAQTGEIGDGRIFVIPIEDAIRIRTAERGDIALYNAEQER
HHHCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEECCCCCEEEECCCCCC
>Mature Secondary Structure
MKKIEAIIRKTKFEDVKDALLEADIEWFSYYDVRGIGKARQGRIYRGVVYDTSTIERILV
CCHHHHHHHHCCHHHHHHHHHHHCHHHHEEECCCCCCCCCCCCEEEEEEECHHHHHHHHH
SIVVRDKNAEKTVQAIIKAAQTGEIGDGRIFVIPIEDAIRIRTAERGDIALYNAEQER
HHHCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEECCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]