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The map label for this gene is amtB [C]

Identifier: 29345954

GI number: 29345954

Start: 670232

End: 671689

Strand: Reverse

Name: amtB [C]

Synonym: BT_0544

Alternate gene names: 29345954

Gene position: 671689-670232 (Counterclockwise)

Preceding gene: 29345955

Following gene: 29345953

Centisome position: 10.73

GC content: 47.6

Gene sequence:

>1458_bases
ATGGATAAAACATATAAGAGACACAGCTTCACAAAGCTGTGGATAGCCACTGCTATGTTCATTTTCTGCTGTTTCGCAAC
CAGTGCTTTTGCACAGGAAGCAGTTGATGCAGATACTTTTATGACAACTACGGAGACAATTACTGAAGTCAAAACTACAC
CTGAAATTGCTGAAACCGCCACTGCTTCTGCCGAAACAACTCCCGATACAATCGGGGAACTGGCATTAGGATTGAATACA
GTCTGGATGCTGCTTGCGGCAATGCTCGTATTTTTTATGCAGCCGGGGTTTGCATTGGTTGAGGCGGGTTTTACCAGAGT
CAAGAATACCGCCAACATTTTGATGAAGAACTTTGTGGATTTCATGTTTGGTTCCTTGCTCTACTGGTTCATCGGTTTCG
GTCTGATGTTTGGTGCGGGAGGTCTCATCGGAATGCCACACTTCTTTGATCTGTCATTCCTCGACAGTGATTTACCCAGA
GAAGGATTTCTGGTTTTTCAGACAGTATTCTGCGCAACGGCAGCCACGATTGTATCGGGAGCGATGGCGGAACGCACCAA
GTTCTCCATGTATCTGGTCTATACTATCTTTATCAGTGTACTAATTTACCCAATTTCCGGTCACTGGACATGGGGTGGCG
GCTGGCTGATGAACGGAGAAGAAGGTTCGTTCATGATGAATCTTTTCGGAACGACATTCCATGACTTTGCAGGTTCAACG
ATTGTCCACTCCGTTGGTGGTTGGATTGCTTTGGTAGGCGCTGCTATTCTTGGTCCCCGTATCGGTAAATATGGCAAAGA
CGGTAAGTCAAGAGCTATTCCGGGACACAACCTGACAGTTGCCGCACTGGGTGTATTTATCCTTTGGTTCGGATGGTTCG
GATTCAACCCCGGATCACAGTTGGCAGCAAGTACCGAAGCGGATGCAATGGCCATCTCACATGTATTTCTGACAACAAAT
CTGGCGGCTTGTGCCGGTGGCTTCTTTGCACTGGCGATGAGCTGGATGAAATATGGCAAACCGTCTTTATCACTGACACT
GAATGGTGTTTTGGCAGGTTTGGTAGGTATCACAGCCGGATGTGACGCTGTAGCTCCCTCAGGAGCGGTATTAATCGGTG
CCATTTGTGGTGTAGTCATGATATTTGCAGTCGACTTTATTGACAAGGTATTGAAGATTGACGATCCTGTCGGTGCTTCT
TCCGTACACGGGGTTTGTGGTTTCTTGGGAACAGTGCTCACAGGATTGTTTTCTACCAGTGAAGGGTTGTTCTACGGACA
TGGAGCCGGCTTTTTGGGTGCCCAGCTTTTCGGTGCGGTTGTTGTCGGAGTCTGGGCGGCAGGTATGGGATTCATTATCT
TCAAAGTACTCGACAAGATTCACGGACTTCGCGTTCCGGCACGTGTCGAAGAAGAAGGTCTCGACATCTACGAACACGGA
GAATCTGCTTATAACTAA

Upstream 100 bases:

>100_bases
GCCGAACAGGAACGATAACCGGCAACCGGCAAGGTGCCGGGGCCAGATTTCCTTTCGGATGATAATATTATAAAAACAGG
TATTAAATAAGATAATCATT

Downstream 100 bases:

>100_bases
GCTAAAAAAGGAATTTTTACTCTAACATATTTAACAACAAATCAAATTAGGTATTATGTCAAAACTAAGATTCAGAGTCG
TAGAGACAGCTTTCAAGAAG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MDKTYKRHSFTKLWIATAMFIFCCFATSAFAQEAVDADTFMTTTETITEVKTTPEIAETATASAETTPDTIGELALGLNT
VWMLLAAMLVFFMQPGFALVEAGFTRVKNTANILMKNFVDFMFGSLLYWFIGFGLMFGAGGLIGMPHFFDLSFLDSDLPR
EGFLVFQTVFCATAATIVSGAMAERTKFSMYLVYTIFISVLIYPISGHWTWGGGWLMNGEEGSFMMNLFGTTFHDFAGST
IVHSVGGWIALVGAAILGPRIGKYGKDGKSRAIPGHNLTVAALGVFILWFGWFGFNPGSQLAASTEADAMAISHVFLTTN
LAACAGGFFALAMSWMKYGKPSLSLTLNGVLAGLVGITAGCDAVAPSGAVLIGAICGVVMIFAVDFIDKVLKIDDPVGAS
SVHGVCGFLGTVLTGLFSTSEGLFYGHGAGFLGAQLFGAVVVGVWAAGMGFIIFKVLDKIHGLRVPARVEEEGLDIYEHG
ESAYN

Sequences:

>Translated_485_residues
MDKTYKRHSFTKLWIATAMFIFCCFATSAFAQEAVDADTFMTTTETITEVKTTPEIAETATASAETTPDTIGELALGLNT
VWMLLAAMLVFFMQPGFALVEAGFTRVKNTANILMKNFVDFMFGSLLYWFIGFGLMFGAGGLIGMPHFFDLSFLDSDLPR
EGFLVFQTVFCATAATIVSGAMAERTKFSMYLVYTIFISVLIYPISGHWTWGGGWLMNGEEGSFMMNLFGTTFHDFAGST
IVHSVGGWIALVGAAILGPRIGKYGKDGKSRAIPGHNLTVAALGVFILWFGWFGFNPGSQLAASTEADAMAISHVFLTTN
LAACAGGFFALAMSWMKYGKPSLSLTLNGVLAGLVGITAGCDAVAPSGAVLIGAICGVVMIFAVDFIDKVLKIDDPVGAS
SVHGVCGFLGTVLTGLFSTSEGLFYGHGAGFLGAQLFGAVVVGVWAAGMGFIIFKVLDKIHGLRVPARVEEEGLDIYEHG
ESAYN
>Mature_485_residues
MDKTYKRHSFTKLWIATAMFIFCCFATSAFAQEAVDADTFMTTTETITEVKTTPEIAETATASAETTPDTIGELALGLNT
VWMLLAAMLVFFMQPGFALVEAGFTRVKNTANILMKNFVDFMFGSLLYWFIGFGLMFGAGGLIGMPHFFDLSFLDSDLPR
EGFLVFQTVFCATAATIVSGAMAERTKFSMYLVYTIFISVLIYPISGHWTWGGGWLMNGEEGSFMMNLFGTTFHDFAGST
IVHSVGGWIALVGAAILGPRIGKYGKDGKSRAIPGHNLTVAALGVFILWFGWFGFNPGSQLAASTEADAMAISHVFLTTN
LAACAGGFFALAMSWMKYGKPSLSLTLNGVLAGLVGITAGCDAVAPSGAVLIGAICGVVMIFAVDFIDKVLKIDDPVGAS
SVHGVCGFLGTVLTGLFSTSEGLFYGHGAGFLGAQLFGAVVVGVWAAGMGFIIFKVLDKIHGLRVPARVEEEGLDIYEHG
ESAYN

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=421, Percent_Identity=35.1543942992874, Blast_Score=206, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17550180, Length=467, Percent_Identity=36.4025695931477, Blast_Score=241, Evalue=4e-64,
Organism=Caenorhabditis elegans, GI17550182, Length=432, Percent_Identity=37.5, Blast_Score=228, Evalue=4e-60,
Organism=Caenorhabditis elegans, GI32564042, Length=432, Percent_Identity=32.4074074074074, Blast_Score=196, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI133931100, Length=455, Percent_Identity=33.4065934065934, Blast_Score=190, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6324187, Length=434, Percent_Identity=30.4147465437788, Blast_Score=147, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6325396, Length=430, Percent_Identity=28.8372093023256, Blast_Score=127, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6321559, Length=448, Percent_Identity=26.7857142857143, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI161078314, Length=418, Percent_Identity=36.3636363636364, Blast_Score=212, Evalue=5e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 51719; Mature: 51719

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKTYKRHSFTKLWIATAMFIFCCFATSAFAQEAVDADTFMTTTETITEVKTTPEIAETA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHH
TASAETTPDTIGELALGLNTVWMLLAAMLVFFMQPGFALVEAGFTRVKNTANILMKNFVD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
FMFGSLLYWFIGFGLMFGAGGLIGMPHFFDLSFLDSDLPREGFLVFQTVFCATAATIVSG
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
AMAERTKFSMYLVYTIFISVLIYPISGHWTWGGGWLMNGEEGSFMMNLFGTTFHDFAGST
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEECCCCCCEEHHHHHHHHHHHCCCH
IVHSVGGWIALVGAAILGPRIGKYGKDGKSRAIPGHNLTVAALGVFILWFGWFGFNPGSQ
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
LAASTEADAMAISHVFLTTNLAACAGGFFALAMSWMKYGKPSLSLTLNGVLAGLVGITAG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCC
CDAVAPSGAVLIGAICGVVMIFAVDFIDKVLKIDDPVGASSVHGVCGFLGTVLTGLFSTS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCC
EGLFYGHGAGFLGAQLFGAVVVGVWAAGMGFIIFKVLDKIHGLRVPARVEEEGLDIYEHG
CCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCC
ESAYN
CCCCC
>Mature Secondary Structure
MDKTYKRHSFTKLWIATAMFIFCCFATSAFAQEAVDADTFMTTTETITEVKTTPEIAETA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHH
TASAETTPDTIGELALGLNTVWMLLAAMLVFFMQPGFALVEAGFTRVKNTANILMKNFVD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
FMFGSLLYWFIGFGLMFGAGGLIGMPHFFDLSFLDSDLPREGFLVFQTVFCATAATIVSG
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
AMAERTKFSMYLVYTIFISVLIYPISGHWTWGGGWLMNGEEGSFMMNLFGTTFHDFAGST
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEECCCCCCEEHHHHHHHHHHHCCCH
IVHSVGGWIALVGAAILGPRIGKYGKDGKSRAIPGHNLTVAALGVFILWFGWFGFNPGSQ
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
LAASTEADAMAISHVFLTTNLAACAGGFFALAMSWMKYGKPSLSLTLNGVLAGLVGITAG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCC
CDAVAPSGAVLIGAICGVVMIFAVDFIDKVLKIDDPVGASSVHGVCGFLGTVLTGLFSTS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCC
EGLFYGHGAGFLGAQLFGAVVVGVWAAGMGFIIFKVLDKIHGLRVPARVEEEGLDIYEHG
CCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCC
ESAYN
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]