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The map label for this gene is mtbA [H]

Identifier: 29345753

GI number: 29345753

Start: 411895

End: 412911

Strand: Reverse

Name: mtbA [H]

Synonym: BT_0343

Alternate gene names: 29345753

Gene position: 412911-411895 (Counterclockwise)

Preceding gene: 29345756

Following gene: 29345752

Centisome position: 6.6

GC content: 44.35

Gene sequence:

>1017_bases
ATGGGTAAACTGAATATGAAAGACTGGATCGGTCAGACGATCCTAAATAAAAAGGTCATTTCCATTCCTATCATGACACA
TCCGGGCATCGAGCTTATCGGAAAGACGGTTCATGATGCAGTAACGAACGGACAGGTGCATTATGAAGCAATCAAAGCGC
TCTGCGACAAATATCCTGCTGCTGCCGCAACGGTTATCATGGATTTAACGGTAGAGGCGGAAGCATTCGGAGCCGAAATT
ATATTTCCGGAGAATGAAGTACCAAGCGTATCCGGTCGTTTGCTTGCAGACGAAGCTGCCATTGATGCCTTGGAAATACC
AGCACTGAATAAAGGAAGGATCGCGCAATATCTCAAAGCGAATATGCTGACTGCAAAGAATATCACAGACCGTCCGGTGC
TGGCTGGTTGTATCGGTCCTTATTCATTAGCGGGACGCTTGTATGATATGTCCGAAATCATGATGCTGATTTATATCAAT
CTCGAAGCAGCCAACACTTTGCTCCACAAATGTACCGATTTCATTATTCGATACTGCATGGCCCTGAAAGCGACCGGAGT
GAATGGAGTGGTAATGGCGGAACCTGCTGCCGGATTATTATCCAATGAAGATTGCCTGCAGTATTCTTCTGTATTCGTAA
AAGAAATAGTTGAGAAGGTTCAGGATGATCATTTCACCGTTGTACTTCATAATTGCGGGAATACAGGAAATTGCACCCAG
GCTATGGTATATACGGGTGCGGCAGCCTATCATTTCGGAAATAAAATCAACATGGAAGAAGCATTGAAAGAAGTTCCTGC
CGATGCTCTTGCGATGGGTAATCTTGATCCGGTCAGTCTTTTCAAGATGTCGAGTCCGGAGGAAATGAAAAAAGCGACTC
TACAGTTATTGGAAGCCACTGCCGCATATCCGAACTTCGTTCTGTCGAGCGGTTGCGATATACCGCCATATACGCCATTA
GCTAATATAAATGCTTTTTATACAGCATTGGAAGAGTTCAATGAAACTCGAAATTAG

Upstream 100 bases:

>100_bases
TAGTTTTTTATAATAATCATCTATTTTTTATGCAAACTTCAAGATTACAAAAGAACAATAATATCGTCTTACTATATGAC
GAAGTTAAAAAACAGAAGTT

Downstream 100 bases:

>100_bases
GCTGTCATTCAGATAATGTCAATTAATAATGATTGAGCAAGAACTCACTTATCAAGACTTACGAATCCCATCGTCCGAAA
TTTATGAAGCAATGGGATAC

Product: methylcobamide:CoM methyltransferase mtbA

Products: CO2; Coproporphyrinogen III [C]

Alternate protein names: MT2-A; Methylcobamide:CoM methyltransferase II isozyme A [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MGKLNMKDWIGQTILNKKVISIPIMTHPGIELIGKTVHDAVTNGQVHYEAIKALCDKYPAAAATVIMDLTVEAEAFGAEI
IFPENEVPSVSGRLLADEAAIDALEIPALNKGRIAQYLKANMLTAKNITDRPVLAGCIGPYSLAGRLYDMSEIMMLIYIN
LEAANTLLHKCTDFIIRYCMALKATGVNGVVMAEPAAGLLSNEDCLQYSSVFVKEIVEKVQDDHFTVVLHNCGNTGNCTQ
AMVYTGAAAYHFGNKINMEEALKEVPADALAMGNLDPVSLFKMSSPEEMKKATLQLLEATAAYPNFVLSSGCDIPPYTPL
ANINAFYTALEEFNETRN

Sequences:

>Translated_338_residues
MGKLNMKDWIGQTILNKKVISIPIMTHPGIELIGKTVHDAVTNGQVHYEAIKALCDKYPAAAATVIMDLTVEAEAFGAEI
IFPENEVPSVSGRLLADEAAIDALEIPALNKGRIAQYLKANMLTAKNITDRPVLAGCIGPYSLAGRLYDMSEIMMLIYIN
LEAANTLLHKCTDFIIRYCMALKATGVNGVVMAEPAAGLLSNEDCLQYSSVFVKEIVEKVQDDHFTVVLHNCGNTGNCTQ
AMVYTGAAAYHFGNKINMEEALKEVPADALAMGNLDPVSLFKMSSPEEMKKATLQLLEATAAYPNFVLSSGCDIPPYTPL
ANINAFYTALEEFNETRN
>Mature_337_residues
GKLNMKDWIGQTILNKKVISIPIMTHPGIELIGKTVHDAVTNGQVHYEAIKALCDKYPAAAATVIMDLTVEAEAFGAEII
FPENEVPSVSGRLLADEAAIDALEIPALNKGRIAQYLKANMLTAKNITDRPVLAGCIGPYSLAGRLYDMSEIMMLIYINL
EAANTLLHKCTDFIIRYCMALKATGVNGVVMAEPAAGLLSNEDCLQYSSVFVKEIVEKVQDDHFTVVLHNCGNTGNCTQA
MVYTGAAAYHFGNKINMEEALKEVPADALAMGNLDPVSLFKMSSPEEMKKATLQLLEATAAYPNFVLSSGCDIPPYTPLA
NINAFYTALEEFNETRN

Specific function: Catalyzes the transfer of the methyl group from the methylated corrinoid cofactor of mtmC, mtbC and mttC to coenzyme M [H]

COG id: COG0407

COG function: function code H; Uroporphyrinogen-III decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the uroporphyrinogen decarboxylase family. MtbA/mtaA subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006360
- InterPro:   IPR000257 [H]

Pfam domain/function: PF01208 URO-D [H]

EC number: 4.1.1.37 [C]

Molecular weight: Translated: 36734; Mature: 36603

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKLNMKDWIGQTILNKKVISIPIMTHPGIELIGKTVHDAVTNGQVHYEAIKALCDKYPA
CCCCCHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCH
AAATVIMDLTVEAEAFGAEIIFPENEVPSVSGRLLADEAAIDALEIPALNKGRIAQYLKA
HHHHHHHEEEEEHHHCCCEEEECCCCCCCCCCCEEEHHHHHCHHCCCCCCCHHHHHHHHH
NMLTAKNITDRPVLAGCIGPYSLAGRLYDMSEIMMLIYINLEAANTLLHKCTDFIIRYCM
HHHEECCCCCCCHHHHHCCHHHHHHHHHHHHHEEEEEEEEHHHHHHHHHHHHHHHHHHHH
ALKATGVNGVVMAEPAAGLLSNEDCLQYSSVFVKEIVEKVQDDHFTVVLHNCGNTGNCTQ
HHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHH
AMVYTGAAAYHFGNKINMEEALKEVPADALAMGNLDPVSLFKMSSPEEMKKATLQLLEAT
HHHHCCHHHHHCCCCCCHHHHHHHCCHHHHHCCCCCHHHEEECCCHHHHHHHHHHHHHHH
AAYPNFVLSSGCDIPPYTPLANINAFYTALEEFNETRN
HHCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GKLNMKDWIGQTILNKKVISIPIMTHPGIELIGKTVHDAVTNGQVHYEAIKALCDKYPA
CCCCHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCH
AAATVIMDLTVEAEAFGAEIIFPENEVPSVSGRLLADEAAIDALEIPALNKGRIAQYLKA
HHHHHHHEEEEEHHHCCCEEEECCCCCCCCCCCEEEHHHHHCHHCCCCCCCHHHHHHHHH
NMLTAKNITDRPVLAGCIGPYSLAGRLYDMSEIMMLIYINLEAANTLLHKCTDFIIRYCM
HHHEECCCCCCCHHHHHCCHHHHHHHHHHHHHEEEEEEEEHHHHHHHHHHHHHHHHHHHH
ALKATGVNGVVMAEPAAGLLSNEDCLQYSSVFVKEIVEKVQDDHFTVVLHNCGNTGNCTQ
HHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHH
AMVYTGAAAYHFGNKINMEEALKEVPADALAMGNLDPVSLFKMSSPEEMKKATLQLLEAT
HHHHCCHHHHHCCCCCCHHHHHHHCCHHHHHCCCCCHHHEEECCCHHHHHHHHHHHHHHH
AAYPNFVLSSGCDIPPYTPLANINAFYTALEEFNETRN
HHCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Reducing agents [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.00231 {7-COOH,} 0.00533 {uroporphyrinogen} 0.00256 {uroporphyrinogen} 0.00133 {uroporphyrinogen} 0.001 {uroporphyrinogen} 0.0008 {uroporphyrinogen} 0.0004 {uroporphyrinogen} 0.00035 {uroporphyrinogen} 0.00215 {5-COOH-porphyrinogen}

Substrates: H+; Uroporphyrinogen III [C]

Specific reaction: (4) H+ + Uroporphyrinogen III --> (4) CO2 + Coproporphyrinogen III [C]

General reaction: Decarboxylation [C]

Inhibitor: 5, 5'-Dithiobis (2-nitrobenzoate); 7-COOH-porphyrinogenI; 7-COOH-porphyrinogen III; Butanedione; Diethyl dicarbonate; Fe2+; Iodoacetamide; p-Hydroxy mercuribenzoate; Phenyl glyoxal; Pt2+; Sn2+; UroporphyrinogenI uroporphyrinogen III; Uroporphyrinogen III;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]